##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938585_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2932007 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.806960897433054 31.0 31.0 34.0 30.0 34.0 2 32.04363563934192 31.0 31.0 34.0 30.0 34.0 3 32.1176750260146 33.0 31.0 34.0 30.0 34.0 4 35.78239478964409 37.0 35.0 37.0 35.0 37.0 5 35.6523422351993 37.0 35.0 37.0 33.0 37.0 6 35.689010292267376 37.0 35.0 37.0 33.0 37.0 7 35.62048282967947 37.0 35.0 37.0 33.0 37.0 8 35.60148082866105 37.0 35.0 37.0 33.0 37.0 9 37.177353601133966 39.0 37.0 39.0 33.0 39.0 10-11 37.10846972739151 39.0 37.0 39.0 33.0 39.0 12-13 37.002945081645436 39.0 37.0 39.0 33.0 39.0 14-15 38.074333894837224 40.0 37.0 41.0 33.0 41.0 16-17 37.991456705253434 40.0 37.0 41.0 33.0 41.0 18-19 37.970922647865436 40.0 37.0 41.0 33.0 41.0 20-21 37.947874953913825 40.0 37.0 41.0 32.5 41.0 22-23 37.98658461593031 40.0 37.0 41.0 33.0 41.0 24-25 37.92053344347404 40.0 37.0 41.0 33.0 41.0 26-27 37.75516310158878 40.0 37.0 41.0 32.0 41.0 28-29 37.630003952923715 40.0 37.0 41.0 32.0 41.0 30-31 37.40704762983171 39.0 36.0 41.0 31.5 41.0 32-33 37.20969458804157 39.0 36.0 41.0 31.0 41.0 34-35 37.06490093645752 39.0 36.0 41.0 31.0 41.0 36-37 37.099711392230645 39.0 36.0 41.0 31.0 41.0 38-39 36.970164805199985 39.0 36.0 41.0 31.0 41.0 40-41 36.812805358240965 39.0 35.5 41.0 30.0 41.0 42-43 36.60469824935616 39.0 35.0 40.0 30.0 41.0 44-45 36.36149095141997 39.0 35.0 40.0 30.0 41.0 46-47 36.041329710331524 38.5 35.0 40.0 29.5 41.0 48-49 35.695859184510816 38.0 34.5 40.0 28.0 41.0 50-51 35.34288219639312 38.0 34.0 40.0 27.0 41.0 52-53 34.8345425164401 38.0 33.5 40.0 26.0 41.0 54-55 34.515116607838934 38.0 33.0 40.0 25.5 41.0 56-57 34.150735997560716 37.0 33.0 40.0 25.0 41.0 58-59 33.69760611076303 37.0 32.5 39.5 23.5 41.0 60-61 33.28250614681343 36.0 32.0 39.0 22.5 40.5 62-63 32.7998575719635 36.0 31.0 39.0 22.0 40.0 64-65 32.297859282054915 35.0 31.0 38.5 20.0 40.0 66-67 31.803606028225715 35.0 30.5 38.0 18.5 40.0 68-69 31.235246027720944 34.5 29.5 37.5 17.5 40.0 70-71 30.66187461353264 34.0 29.0 37.0 13.0 39.0 72-73 30.075678025325317 34.0 29.0 36.0 8.0 39.0 74-75 29.457387038980468 34.0 28.0 36.0 7.0 38.5 76-77 28.18061672431205 31.5 26.5 34.5 7.0 36.5 78-79 28.807391660388262 33.0 28.0 35.0 2.0 37.0 80-81 28.51079209565325 33.0 27.0 35.0 2.0 37.0 82-83 28.014079945921004 33.0 26.0 35.0 2.0 36.0 84-85 27.430651086440108 33.0 26.0 35.0 2.0 36.0 86-87 26.784816509646802 32.0 25.0 35.0 2.0 35.0 88-89 26.03847995588005 32.0 23.5 34.0 2.0 35.0 90-91 25.49651518567316 31.0 21.5 34.0 2.0 35.0 92-93 24.718194908811608 31.0 18.5 34.0 2.0 35.0 94-95 23.931509713312415 31.0 11.0 34.0 2.0 35.0 96-97 22.95128882707306 30.5 2.0 34.0 2.0 35.0 98-99 21.77210286332877 30.0 2.0 34.0 2.0 35.0 100-101 18.591802986827794 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.306230725433835 1101 2 0.37509782195274965 1101 3 0.3600241256497796 1101 4 0.2713791136699726 1101 5 0.20825640678923918 1101 6 0.2655189766029977 1101 7 0.36235622344161555 1101 8 0.34034152223654246 1101 9 0.41623993490262734 1101 10-11 0.44130132632151486 1101 12-13 0.3798587532882607 1101 14-15 0.5750909678679292 1101 16-17 0.6047013403578063 1101 18-19 0.680206032003511 1101 20-21 0.5688054995592253 1101 22-23 0.608983459454052 1101 24-25 0.5916194189092749 1101 26-27 0.5591571752225093 1101 28-29 0.588602343409093 1101 30-31 0.7330881996840617 1101 32-33 0.7007644672819495 1101 34-35 0.5518238844549472 1101 36-37 0.706575481930976 1101 38-39 0.8403490496946446 1101 40-41 0.9051380908133027 1101 42-43 0.8192956188675211 1101 44-45 0.9289223565677318 1101 46-47 0.9687385258280159 1101 48-49 1.1042990998128488 1101 50-51 1.0964881622208438 1101 52-53 1.0397230199322607 1101 54-55 1.1294850910157663 1101 56-57 1.3245327654338794 1101 58-59 1.5658175749422156 1101 60-61 1.4494708206566713 1101 62-63 1.4149749552515232 1101 64-65 1.5679009487937208 1101 66-67 1.6962079727568913 1101 68-69 1.5314455173789128 1101 70-71 1.6975075590947029 1101 72-73 1.6688101139877745 1101 74-75 1.79450294015545 1101 76-77 1.7320190638529382 1101 78-79 1.9499733341708385 1101 80-81 2.317862223371165 1101 82-83 2.308266450093331 1101 84-85 2.5660908144489483 1101 86-87 2.5982831447061407 1101 88-89 2.8059043023815633 1101 90-91 3.0525534532049114 1101 92-93 3.402336956166071 1101 94-95 3.4468391247587498 1101 96-97 3.7873247685086433 1101 98-99 4.099265049847144 1101 100-101 4.515436665971539 1102 1 0.3915396097220949 1102 2 0.40355789657849783 1102 3 0.4079775935962573 1102 4 0.3167047275100927 1102 5 0.18361556935567336 1102 6 0.2504142227215951 1102 7 0.27784521928013817 1102 8 0.3339749717805134 1102 9 0.41910056877340196 1102 10-11 0.39912469212810464 1102 12-13 0.428167724211697 1102 14-15 0.5300624230700706 1102 16-17 0.501663193992151 1102 18-19 0.6132157082568099 1102 20-21 0.6465416327025579 1102 22-23 0.618464873220681 1102 24-25 0.552866129013033 1102 26-27 0.517407742085453 1102 28-29 0.5175653800617539 1102 30-31 0.503053814480154 1102 32-33 0.44612085154781056 1102 34-35 0.4971304983040241 1102 36-37 0.7193991614166535 1102 38-39 0.8789541038117576 1102 40-41 0.8150912181938992 1102 42-43 0.8822171961057634 1102 44-45 0.7498528781413825 1102 46-47 0.7701244497025783 1102 48-49 0.9348060948567181 1102 50-51 0.9880550341232635 1102 52-53 0.9850854985348647 1102 54-55 0.9794664196409215 1102 56-57 1.1603334686725404 1102 58-59 1.2189226693894994 1102 60-61 1.2759623647644318 1102 62-63 1.2996496102792037 1102 64-65 1.2410612507621295 1102 66-67 1.3095887463194238 1102 68-69 1.3514264576394766 1102 70-71 1.4979735368623253 1102 72-73 1.555462261793366 1102 74-75 1.6576303506299404 1102 76-77 1.8171674041917711 1102 78-79 1.9913152832233756 1102 80-81 2.206335203651207 1102 82-83 2.1609999094957892 1102 84-85 2.401642964345733 1102 86-87 2.4130090937347823 1102 88-89 2.4722522113369543 1102 90-91 2.665098346658578 1102 92-93 2.8698826412860328 1102 94-95 3.1779087704806166 1102 96-97 3.4783215211831298 1102 98-99 3.8598916313125926 1102 100-101 4.259014758623383 1103 1 0.27270843636646447 1103 2 0.26107032225668547 1103 3 0.309799639304174 1103 4 0.23488500435884418 1103 5 0.1707480247625881 1103 6 0.2151301740408087 1103 7 0.22072727985241158 1103 8 0.14583766223016426 1103 9 0.3635559292341952 1103 10-11 0.38716880909206 1103 12-13 0.3601481682845318 1103 14-15 0.5095030933599247 1103 16-17 0.4908145898487106 1103 18-19 0.4529478594087948 1103 20-21 0.4549796552778034 1103 22-23 0.3988014697461324 1103 24-25 0.4008058470546487 1103 26-27 0.41160750518466216 1103 28-29 0.37714737792499875 1103 30-31 0.3475153297161242 1103 32-33 0.572096499541118 1103 34-35 0.605324187917553 1103 36-37 0.7113058062512891 1103 38-39 0.7263239245103819 1103 40-41 0.752690159108063 1103 42-43 0.7209933829322281 1103 44-45 0.8070479264505579 1103 46-47 0.7053132735598808 1103 48-49 0.9031157381120138 1103 50-51 0.8713939415926077 1103 52-53 0.9570197237299638 1103 54-55 0.9316208812722877 1103 56-57 0.942903900203568 1103 58-59 1.0885045511249132 1103 60-61 1.1486682594132631 1103 62-63 1.0397965700525162 1103 64-65 1.1165485812154898 1103 66-67 1.1360459764383357 1103 68-69 1.263590732072771 1103 70-71 1.3227444166467919 1103 72-73 1.2224943827521173 1103 74-75 1.4634204263452908 1103 76-77 1.337219197205524 1103 78-79 1.4995858494626937 1103 80-81 1.8353688458845738 1103 82-83 2.0938598477157804 1103 84-85 2.2040126108526046 1103 86-87 2.288118892601375 1103 88-89 2.5443931440757126 1103 90-91 2.73951521074256 1103 92-93 2.873557042066409 1103 94-95 2.8586668529778265 1103 96-97 3.2401485023239722 1103 98-99 3.4952591178417656 1103 100-101 3.9150400512655246 1104 1 0.28818789419298696 1104 2 0.2825039840207495 1104 3 0.17720494288362687 1104 4 0.20589730737289358 1104 5 0.16799155793306397 1104 6 0.1751813958248789 1104 7 0.15986754581063423 1104 8 0.18638469239963484 1104 9 0.25641944714595866 1104 10-11 0.2719917731779091 1104 12-13 0.3480977293701102 1104 14-15 0.45774053799726744 1104 16-17 0.4997426677768644 1104 18-19 0.5494872289631374 1104 20-21 0.5543016552741804 1104 22-23 0.5000834754328167 1104 24-25 0.44109650780026044 1104 26-27 0.4814533641304024 1104 28-29 0.5509904818763118 1104 30-31 0.5669787695221444 1104 32-33 0.5741029994432623 1104 34-35 0.5383924004195464 1104 36-37 0.6151506227299777 1104 38-39 0.5956421166251431 1104 40-41 0.7812300165967869 1104 42-43 0.7389839246164129 1104 44-45 0.6830987749217954 1104 46-47 0.7811105048833653 1104 48-49 0.9621847937392474 1104 50-51 0.8101217059198405 1104 52-53 0.9809824214407925 1104 54-55 0.9928403933016625 1104 56-57 1.0718858094812944 1104 58-59 1.2344564513054124 1104 60-61 1.1303035554177256 1104 62-63 1.266049920354071 1104 64-65 1.3842259523863234 1104 66-67 1.5662122931997153 1104 68-69 1.5331091267913024 1104 70-71 1.3998932080917683 1104 72-73 1.6709209189525893 1104 74-75 1.7884143441548481 1104 76-77 1.7753623010662274 1104 78-79 2.0036666368084752 1104 80-81 2.214500261519497 1104 82-83 2.3289705435817964 1104 84-85 2.5795100026462485 1104 86-87 2.700182368017092 1104 88-89 2.858579847614571 1104 90-91 3.0898041317778357 1104 92-93 3.3945573619194036 1104 94-95 3.606336366003905 1104 96-97 3.969030332952297 1104 98-99 4.372178518964603 1104 100-101 4.595829594337854 1105 1 0.239373460588439 1105 2 0.17498143493344287 1105 3 0.20369763769175364 1105 4 0.18409756732170024 1105 5 0.06251957750156123 1105 6 0.15137113985179695 1105 7 0.1553790132038202 1105 8 0.13516162307546864 1105 9 0.2169369722415695 1105 10-11 0.26332812607883227 1105 12-13 0.31954582064395254 1105 14-15 0.39878138160852217 1105 16-17 0.422370853761187 1105 18-19 0.43099017606737533 1105 20-21 0.4407407188150003 1105 22-23 0.33669392856165103 1105 24-25 0.2783823857206258 1105 26-27 0.41561547899354423 1105 28-29 0.48308533944452847 1105 30-31 0.5551508470104025 1105 32-33 0.493681377664295 1105 34-35 0.500744253181324 1105 36-37 0.6154854898495543 1105 38-39 0.5785140464763998 1105 40-41 0.489067975491956 1105 42-43 0.5850666851646409 1105 44-45 0.6578578374820765 1105 46-47 0.6998983340613023 1105 48-49 0.7187063582515663 1105 50-51 0.80527937416349 1105 52-53 0.9924555051828534 1105 54-55 0.8974861055306462 1105 56-57 0.9168283256006049 1105 58-59 1.1302242999691146 1105 60-61 1.1775831266740084 1105 62-63 1.1929545564361348 1105 64-65 1.352042431635006 1105 66-67 1.364733417131962 1105 68-69 1.420704576231671 1105 70-71 1.3860050320418367 1105 72-73 1.4357689106198706 1105 74-75 1.709329345578034 1105 76-77 1.4782138587400304 1105 78-79 1.693809932577235 1105 80-81 1.9150004172983692 1105 82-83 1.9808216200756235 1105 84-85 2.2056089221745054 1105 86-87 2.4544205983953837 1105 88-89 2.6010797816807703 1105 90-91 2.800333284203983 1105 92-93 2.8729957495976954 1105 94-95 2.823764382978826 1105 96-97 3.250873870558582 1105 98-99 3.584403565427415 1105 100-101 4.110841271460725 1106 1 0.1822131953956827 1106 2 0.2087156092362079 1106 3 0.15821161883027912 1106 4 0.10811294891831125 1106 5 0.06894829218839504 1106 6 0.12834259064996445 1106 7 0.19405299298791334 1106 8 0.15072609103163614 1106 9 0.2051312973041277 1106 10-11 0.25228861154202065 1106 12-13 0.05747175351572764 1106 14-15 0.0240902008478443 1106 16-17 0.08014253872149624 1106 18-19 0.2592052279963255 1106 20-21 0.4090401064510232 1106 22-23 0.30913744139324706 1106 24-25 0.16670312535002552 1106 26-27 0.18274097366867892 1106 28-29 0.39948947448584704 1106 30-31 0.4441282955546413 1106 32-33 0.36384673677368795 1106 34-35 0.28906171523524904 1106 36-37 0.30390109466858917 1106 38-39 0.32301596787362996 1106 40-41 0.28611838547106316 1106 42-43 0.425621754510324 1106 44-45 0.013695470733971149 1106 46-47 -0.18584370251556948 1106 48-49 0.35111867116929574 1106 50-51 0.22627949484772358 1106 52-53 0.5011162270655944 1106 54-55 0.43883760401210736 1106 56-57 0.40505721714628606 1106 58-59 0.9760212652380957 1106 60-61 0.7466507042027715 1106 62-63 0.9856401475197458 1106 64-65 0.6375391709900455 1106 66-67 0.702923097153775 1106 68-69 0.49200795947097475 1106 70-71 0.755386540525329 1106 72-73 1.0753472841783882 1106 74-75 1.420446284695874 1106 76-77 1.3473159593403246 1106 78-79 1.618943488351583 1106 80-81 1.6571955007615031 1106 82-83 1.814911183407066 1106 84-85 1.8343296757824454 1106 86-87 2.1009181291438175 1106 88-89 2.4320024148371573 1106 90-91 2.4551766942369255 1106 92-93 2.048496296149686 1106 94-95 2.63847907980092 1106 96-97 3.1725578866694946 1106 98-99 3.70995369887466 1106 100-101 4.02412210498073 1107 1 0.1494242251067135 1107 2 0.16924165726226192 1107 3 0.09099041410907915 1107 4 0.11982438562974806 1107 5 0.05861556935567336 1107 6 0.024623624430994084 1107 7 0.10282385175877096 1107 8 0.16876129656684213 1107 9 0.14722022689305447 1107 10-11 0.16513964939306192 1107 12-13 0.22314635669032867 1107 14-15 0.26634447435211683 1107 16-17 0.27430208288104296 1107 18-19 0.1791558791969763 1107 20-21 0.20820829936922536 1107 22-23 0.21991786467367547 1107 24-25 0.18585758200448055 1107 26-27 0.14362569080339682 1107 28-29 0.16735170484807327 1107 30-31 0.18426107943742664 1107 32-33 0.1566657233426767 1107 34-35 0.2885834897570163 1107 36-37 0.3715038622713607 1107 38-39 0.3524370097946701 1107 40-41 0.41499506434774247 1107 42-43 0.4628368542254222 1107 44-45 0.5803443311328351 1107 46-47 0.569419321497449 1107 48-49 0.5941971204977463 1107 50-51 0.5439204187386508 1107 52-53 0.4830021652015333 1107 54-55 0.5728424880169953 1107 56-57 0.6977159473050207 1107 58-59 0.7162837805006177 1107 60-61 0.7951931339951983 1107 62-63 0.9186453367749294 1107 64-65 0.963358259309139 1107 66-67 1.1276229343536137 1107 68-69 1.3538516713146862 1107 70-71 1.261873109511896 1107 72-73 1.4314879028190042 1107 74-75 1.3197671027666935 1107 76-77 1.4022101392345085 1107 78-79 1.5806956251037185 1107 80-81 1.8222112720272747 1107 82-83 1.9883823881282687 1107 84-85 2.1902540754568456 1107 86-87 2.402635162110851 1107 88-89 2.5598056301403744 1107 90-91 2.8831859534961843 1107 92-93 3.0570941797475726 1107 94-95 3.016658770480614 1107 96-97 3.3773706664822747 1107 98-99 3.611355306526267 1107 100-101 3.7856173227259475 1108 1 0.16040555362159736 1108 2 0.19100272617097858 1108 3 0.11658933979189356 1108 4 0.1447149871982063 1108 5 0.0997155767308513 1108 6 0.04238150972243915 1108 7 0.13045217980656076 1108 8 0.043487800487973516 1108 9 0.09128812767236383 1108 10-11 0.0986685785176249 1108 12-13 0.25141017899777296 1108 14-15 0.28293554547338573 1108 16-17 0.2541919776618755 1108 18-19 0.2587469257343358 1108 20-21 0.3060086533776243 1108 22-23 0.24566091357108633 1108 24-25 0.2622275207905318 1108 26-27 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-0.0187176685141921 2316 58-59 0.08362787002690908 2316 60-61 0.396705519914363 2316 62-63 0.49548040848287656 2316 64-65 0.511094569609007 2316 66-67 0.5924121564309708 2316 68-69 0.6088000633181636 2316 70-71 0.5920616145097242 2316 72-73 0.6703340566651583 2316 74-75 0.7471334652178037 2316 76-77 0.829893755053714 2316 78-79 0.962639345225405 2316 80-81 1.003933349384944 2316 82-83 1.2547910148123194 2316 84-85 1.3640230889292475 2316 86-87 1.4545359661091126 2316 88-89 1.5541803585193392 2316 90-91 1.8562862359820578 2316 92-93 1.9828074565837284 2316 94-95 2.5121156370603615 2316 96-97 2.528740179737383 2316 98-99 2.885735467325919 2316 100-101 3.3778543935647143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 19.0 4 40.0 5 95.0 6 168.0 7 450.0 8 1201.0 9 1938.0 10 3241.0 11 4938.0 12 7187.0 13 10013.0 14 13155.0 15 17001.0 16 21469.0 17 26196.0 18 26459.0 19 24656.0 20 26046.0 21 27864.0 22 31272.0 23 35367.0 24 41120.0 25 47576.0 26 56703.0 27 68106.0 28 81082.0 29 96290.0 30 114301.0 31 135829.0 32 161783.0 33 196397.0 34 244906.0 35 310346.0 36 396150.0 37 445705.0 38 243738.0 39 13193.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69708179314776 18.698836223391087 14.46596630429815 24.138115679163004 2 16.172646533260586 19.993032502590633 38.816740146126364 25.01758081802241 3 19.753349165535187 22.914127869757117 29.394518893082843 27.938004071624857 4 13.614565040260818 16.345322504346 35.64060385940416 34.399508595989026 5 14.209146127250435 36.87992972448681 34.12937263126557 14.78155151699719 6 34.04886141131313 35.28334004659607 16.543957773634236 14.123840768456555 7 30.069914567052535 31.584235644730725 19.758308898989668 18.58754088922707 8 27.031893170787107 35.08265839747313 19.038494792133854 18.84695363960591 9 26.329950781154345 16.495219827237793 19.396099668247725 37.778729723360144 10-11 25.463360080654653 25.98902390069328 27.08992509226615 21.45769092638592 12-13 26.272754464774472 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23.078924920350637 38-39 22.87171872684222 26.344210634571493 27.590161940923824 23.19390869766246 40-41 23.233409714012453 26.045840004420484 27.651300938738782 23.06944934282828 42-43 22.923896145180095 26.11497403356638 27.531190076476996 23.42993974477653 44-45 23.068896073764368 26.24143034768389 27.667402075185617 23.022271503366127 46-47 23.34880056480122 26.502802689627064 27.05082358317943 23.097573162392287 48-49 23.146670522955777 26.4377097326166 27.12442364564614 23.291196098781484 50-51 23.249535215980043 26.726709724772146 27.09161335562978 22.932141703618033 52-53 23.482208603185466 26.451130573699178 27.03020490742348 23.036455915691878 54-55 22.984409655229335 26.5370614735913 27.215419335629143 23.26310953555022 56-57 23.148802168616925 26.625106965979278 26.997104713597203 23.228986151806595 58-59 23.165650695922622 26.380701001054906 26.97367366448989 23.479974638532582 60-61 22.858864252370477 26.55491613764906 27.313969577835252 23.272250032145216 62-63 22.859716910634933 26.60817317284713 27.38045986929772 23.151650047220215 64-65 22.581341040454543 26.59655996728521 27.223212632166295 23.598886360093957 66-67 22.65379127534553 26.595126230324233 27.086377684322464 23.664704810007773 68-69 23.040922428848447 26.282892027400422 27.222097828494796 23.454087715256332 70-71 22.835279724775553 26.424169519377 26.94754821526688 23.793002540580567 72-73 22.811630874624598 26.438055304447655 26.969432025474703 23.780881795453045 74-75 22.72929430250337 26.810713616986593 26.726488033623387 23.733504046886654 76-77 22.762155752015598 26.406911716104357 27.05283445776221 23.778098074117832 78-79 22.829464595411945 26.455189227038 27.114873873084207 23.60047230446585 80-81 22.9716997461294 26.520891587686314 26.938404447058506 23.569004219125784 82-83 22.820792451510968 26.577199177005962 27.007591292907264 23.59441707857581 84-85 22.782945206308785 26.562176242236426 26.95230141620759 23.702577135247203 86-87 22.838082530886243 26.68592335048563 26.726408089580755 23.74958602904737 88-89 22.65351776224196 26.732602184306582 26.580914227324225 24.03296582612723 90-91 22.67601824430769 26.566149940159956 26.743640737343977 24.01419107818838 92-93 22.728995875343173 26.321644105570886 26.907387325688614 24.041972693397327 94-95 22.478084124628626 26.203433347874 27.043472270018455 24.27501025747892 96-97 22.56007959054661 26.00597918183333 27.031369132367207 24.402572095252857 98-99 22.381085720463833 26.20300701874177 26.71037279242512 24.70553446836928 100-101 22.533406980834993 25.854502300404388 26.95189896126668 24.660191757493944 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 199.0 1 141.0 2 122.5 3 173.5 4 159.5 5 162.5 6 300.5 7 610.0 8 1014.0 9 1205.5 10 1079.5 11 946.5 12 1008.5 13 1176.5 14 1291.5 15 1440.5 16 1638.0 17 1819.5 18 2046.0 19 2352.5 20 2775.5 21 3282.5 22 3997.5 23 4821.5 24 5915.5 25 7654.5 26 9982.5 27 13051.5 28 16466.5 29 20244.0 30 24722.5 31 30981.0 32 40215.0 33 45324.5 34 47008.5 35 51466.0 36 57575.5 37 63812.5 38 69077.0 39 74205.0 40 80456.0 41 88254.0 42 94105.0 43 98977.0 44 104416.0 45 110857.5 46 115217.0 47 119619.5 48 123485.5 49 122233.0 50 120275.0 51 116868.0 52 113199.0 53 112478.5 54 112334.5 55 105790.0 56 95650.5 57 87056.0 58 79517.5 59 68945.5 60 55142.0 61 45676.5 62 38697.5 63 29304.5 64 20982.0 65 15717.0 66 11421.5 67 8704.0 68 6703.0 69 4800.5 70 3629.0 71 2686.0 72 2003.5 73 1572.5 74 1206.5 75 941.0 76 681.0 77 418.5 78 250.0 79 154.0 80 84.5 81 47.0 82 32.5 83 18.5 84 11.0 85 8.0 86 4.0 87 3.0 88 2.5 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.410633057833764E-5 2 0.04283755120639207 3 2.7285064462670113E-4 4 0.0 5 0.10109116383419274 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.038177603259474E-4 26-27 0.01156204606605646 28-29 0.0 30-31 0.0 32-33 4.945417933858958E-4 34-35 0.014938572793311884 36-37 0.003205995074363738 38-39 0.011988375198285679 40-41 0.006855372446245866 42-43 0.004945417933858958 44-45 0.0026091342892428293 46-47 6.821266115667528E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.8758481818085702E-4 68-69 2.8990380991586993E-4 70-71 0.0 72-73 1.705316528916882E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.1159495867506455E-5 82-83 0.0010061367520609603 84-85 0.004348557148738048 86-87 0.0012960405619768303 88-89 3.410633057833764E-5 90-91 8.185519338801033E-4 92-93 1.8758481818085702E-4 94-95 0.0 96-97 1.705316528916882E-5 98-99 0.0 100-101 7.673924380125968E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2932007.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.61857350714999 #Duplication Level Percentage of deduplicated Percentage of total 1 74.66397237641772 40.78039663577188 2 13.878319109909807 15.160279849205866 3 4.907087387847526 8.040543395974762 4 2.2328843483015275 4.878278316426867 5 1.2119609378965832 3.309778878714949 6 0.7486438903739023 2.4533916814239434 7 0.4917769262235915 1.8802107935844456 8 0.3325935869597913 1.4532629821896035 9 0.2336419412944095 1.1485070590447735 >10 1.1927738385638385 11.474517279961463 >50 0.06785624405628177 2.516136196156301 >100 0.033673675322227925 3.5149404868651724 >500 0.0024391394349788354 0.9336151622541993 >1k 0.002314055361095815 2.276291155141669 >5k 6.254203678637337E-5 0.1798501272839547 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5265 0.17956983049494765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4277 0.14587277588355008 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3187 0.10869687555316206 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3172 0.10818528059448698 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2963 0.10105705750361443 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2946 0.10047724988378269 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.4106330578337635E-5 0.0 8 0.0 0.0 0.0 3.4106330578337635E-5 0.0 9 0.0 0.0 0.0 3.4106330578337635E-5 0.0 10-11 0.0 0.0 0.0 1.0231899173501291E-4 0.0 12-13 0.0 0.0 0.0 1.3642532231335057E-4 0.0 14-15 0.0 0.0 0.0 2.0463798347002582E-4 0.0 16-17 0.0 0.0 0.0 2.3874431404836345E-4 0.0 18-19 0.0 0.0 0.0 2.557974793375323E-4 0.0 20-21 0.0 1.7053165289168817E-5 0.0 2.728506446267011E-4 0.0 22-23 0.0 3.4106330578337635E-5 0.0 3.9222280165088284E-4 0.0 24-25 0.0 3.4106330578337635E-5 0.0 5.968607851209087E-4 0.0 26-27 0.0 3.4106330578337635E-5 0.0 9.549772561934538E-4 0.0 28-29 0.0 3.4106330578337635E-5 0.0 0.003240101404942075 0.0 30-31 0.0 3.4106330578337635E-5 0.0 0.011800790380104823 0.0 32-33 0.0 3.4106330578337635E-5 0.0 0.02914385947918951 0.0 34-35 0.0 3.4106330578337635E-5 0.0 0.05651418976830546 0.0 36-37 0.0 3.4106330578337635E-5 0.0 0.10521802983417161 0.0 38-39 0.0 3.4106330578337635E-5 0.0 0.18064417990816528 0.0 40-41 0.0 3.4106330578337635E-5 0.0 0.2607428972713912 0.0 42-43 0.0 3.4106330578337635E-5 0.0 0.3474411896015255 0.0 44-45 0.0 3.4106330578337635E-5 0.0 0.4555582575348558 0.0 46-47 0.0 3.4106330578337635E-5 0.0 0.580131629972234 0.0 48-49 0.0 3.4106330578337635E-5 0.0 0.7118332255004848 0.0 50-51 0.0 3.4106330578337635E-5 0.0 0.8533915505658751 0.0 52-53 0.0 3.4106330578337635E-5 0.0 0.9922043160197094 0.0 54-55 0.0 3.4106330578337635E-5 0.0 1.1474563328123022 0.0 56-57 0.0 3.4106330578337635E-5 0.0 1.328339257034516 0.0 58-59 0.0 3.4106330578337635E-5 0.0 1.5253885819508617 0.0 60-61 0.0 3.4106330578337635E-5 0.0 1.7422877912638 0.0 62-63 0.0 3.4106330578337635E-5 0.0 1.9568336637668327 0.0 64-65 0.0 3.4106330578337635E-5 0.0 2.179462736616932 0.0 66-67 0.0 3.4106330578337635E-5 0.0 2.439915730078407 0.0 68-69 0.0 3.4106330578337635E-5 0.0 2.727363884192637 0.0 70-71 0.0 3.4106330578337635E-5 0.0 3.0266298818522603 0.0 72-73 0.0 3.4106330578337635E-5 0.0 3.3387198598093386 0.0 74-75 0.0 3.4106330578337635E-5 0.0 3.6574094127333256 0.0 76-77 0.0 3.4106330578337635E-5 0.0 4.0002633008720645 0.0 78-79 0.0 3.4106330578337635E-5 0.0 4.388580927671728 0.0 80-81 0.0 3.4106330578337635E-5 0.0 4.798573127553924 0.0 82-83 0.0 3.4106330578337635E-5 0.0 5.220110320336889 0.0 84-85 0.0 3.4106330578337635E-5 0.0 5.654795503557802 0.0 86-87 0.0 3.4106330578337635E-5 0.0 6.101810125282784 0.0 88-89 0.0 3.4106330578337635E-5 0.0 6.578974743239017 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5360 0.0 36.55877 1 GTATCAA 7970 0.0 32.64286 1 ATCAACG 9700 0.0 26.124714 3 TCAACGC 9855 0.0 25.66514 4 TATCAAC 10160 0.0 25.09171 2 CAACGCA 10085 0.0 25.079817 5 AACGCAG 10575 0.0 24.0729 6 GTACATG 8965 0.0 23.714634 1 TACATGG 9080 0.0 22.73333 2 ACATGGG 9180 0.0 21.389637 3 ACGCAGA 12075 0.0 21.003815 7 CGCAGAG 12175 0.0 20.714266 8 AGAGTAC 10955 0.0 19.747858 10-11 TAAGGTA 895 0.0 19.65474 4 TAAGGTG 1620 0.0 19.369461 5 GTGTAGC 1770 0.0 19.347212 1 CATGGGG 6020 0.0 18.954107 4 GAGTACT 6905 0.0 18.674551 12-13 GTAAGGT 1555 0.0 18.344652 4 AGTACTT 7280 0.0 18.169285 12-13 >>END_MODULE