FastQCFastQC Report
Thu 26 May 2016
SRR938584_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938584_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2960104
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52510.17739241594214258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38260.12925221546269994No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31930.10786783167077912No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA31820.10749622310567467No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31160.10526657171504784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56800.033.761661
GTATCAA88200.033.6409231
ATCAACG105300.028.0283973
TCAACGC106350.027.8857354
TATCAAC106850.027.6218072
CAACGCA107200.027.6198255
AACGCAG111750.026.6599646
GTACATG95450.026.5877631
TACATGG95550.025.9144522
TAAGGTG16250.023.9821535
GTAAGGT14500.023.5993214
ACATGGG99400.023.571953
ACGCAGA125900.023.3553857
CGCAGAG127150.023.01378
GCAGAGT139550.020.5248629
AGGTAAG17000.020.1288322
GTGTAGC19750.020.0473561
AGAGTAC108850.020.0296510-11
CATGGGG64200.019.9877434
AAGGTAA16950.019.9818081
Produced by FastQC (version 0.11.5)