##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938584_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2960104 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.14890997073076 31.0 31.0 34.0 28.0 34.0 2 31.316669617013456 31.0 31.0 34.0 28.0 34.0 3 31.384354063235616 31.0 31.0 34.0 28.0 34.0 4 35.04640580195831 37.0 35.0 37.0 32.0 37.0 5 35.034246431882124 37.0 35.0 37.0 32.0 37.0 6 35.004778548321276 37.0 35.0 37.0 32.0 37.0 7 34.91768127065806 37.0 35.0 37.0 32.0 37.0 8 34.87130418390705 37.0 35.0 37.0 32.0 37.0 9 36.36974072532587 39.0 35.0 39.0 32.0 39.0 10-11 36.20363541280982 39.0 35.0 39.0 32.0 39.0 12-13 36.03100887671515 38.0 35.0 39.0 30.0 39.0 14-15 37.21741854340253 39.0 36.5 41.0 31.5 41.0 16-17 37.14016162945626 39.0 36.0 41.0 31.0 41.0 18-19 37.11312068765152 39.0 36.0 41.0 31.0 41.0 20-21 37.030877293500495 39.0 36.0 41.0 31.0 41.0 22-23 36.881144547624004 39.0 36.0 41.0 30.0 41.0 24-25 36.75313113998698 39.0 36.0 41.0 30.0 41.0 26-27 36.53899643390908 39.0 36.0 41.0 30.0 41.0 28-29 36.314415135414166 39.0 35.5 40.0 29.5 41.0 30-31 36.05921008180793 38.5 35.0 40.0 29.0 41.0 32-33 35.781416125919904 38.0 35.0 40.0 27.5 41.0 34-35 35.49403585144306 38.0 34.0 40.0 27.0 41.0 36-37 35.3038933767192 38.0 34.0 40.0 26.0 41.0 38-39 35.08079091140041 38.0 34.0 40.0 25.0 41.0 40-41 34.823329518152065 38.0 33.5 40.0 25.0 41.0 42-43 34.434090829241136 38.0 33.0 40.0 24.0 41.0 44-45 33.998600387013425 38.0 33.0 40.0 23.0 41.0 46-47 33.55227383902728 37.5 32.5 40.0 21.0 41.0 48-49 33.09187109642094 37.0 31.5 40.0 18.5 41.0 50-51 32.37288858769827 36.5 30.5 39.5 16.0 40.5 52-53 32.37893229427074 36.5 31.0 39.0 15.5 40.0 54-55 32.360067078724256 37.0 31.0 39.0 13.0 40.5 56-57 31.93871465326894 36.0 30.5 39.0 8.5 40.5 58-59 31.484229439235918 36.0 30.0 39.0 7.5 40.0 60-61 30.934189643336858 35.0 29.0 39.0 2.0 40.0 62-63 30.20077588490134 35.0 28.0 38.0 2.0 40.0 64-65 29.542884135152008 34.0 27.5 38.0 2.0 40.0 66-67 28.849274214689753 34.0 26.0 37.5 2.0 39.5 68-69 27.99945339758333 33.5 25.5 37.0 2.0 39.0 70-71 27.15659365346623 32.5 23.0 36.0 2.0 39.0 72-73 26.251490994910988 32.0 21.0 35.5 2.0 38.5 74-75 25.313222947572115 31.0 17.5 35.0 2.0 37.5 76-77 24.367623907808643 30.5 10.5 34.5 2.0 37.0 78-79 23.384550677949154 30.0 4.5 34.0 2.0 36.0 80-81 22.41366536446017 29.0 2.0 34.0 2.0 35.5 82-83 21.401122561909986 28.5 2.0 34.0 2.0 35.0 84-85 20.41844425060741 26.5 2.0 33.5 2.0 35.0 86-87 19.416595666909 25.5 2.0 33.0 2.0 35.0 88-89 18.317059806006817 24.5 2.0 32.0 2.0 34.0 90-91 17.153536666279294 20.0 2.0 31.5 2.0 34.0 92-93 15.97570794809912 12.0 2.0 31.0 2.0 34.0 94-95 14.702355221303035 2.0 2.0 31.0 2.0 34.0 96-97 13.263506788950657 2.0 2.0 30.0 2.0 34.0 98-99 11.528031447543736 2.0 2.0 29.0 2.0 34.0 100-101 8.13623828757368 2.0 2.0 10.5 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.51269200476845 1101 2 0.47412438255084055 1101 3 0.40952399010419427 1101 4 0.3787480896205153 1101 5 0.37799569355861706 1101 6 0.4896738717327267 1101 7 0.3814394609501477 1101 8 0.4025660091375016 1101 9 0.5352152956530745 1101 10-11 0.6004574537113498 1101 12-13 0.5497435533057171 1101 14-15 0.6687075885079565 1101 16-17 0.7348432294189777 1101 18-19 0.5821882660667086 1101 20-21 0.7943065237218363 1101 22-23 0.9072830481471854 1101 24-25 0.8506501226558356 1101 26-27 0.7917833124178983 1101 28-29 0.9474694652691156 1101 30-31 0.9439786265026768 1101 32-33 0.8967054255555951 1101 34-35 1.0095344641106152 1101 36-37 1.0977800987284567 1101 38-39 1.2404687498051459 1101 40-41 1.1923415790082998 1101 42-43 1.283852721597306 1101 44-45 1.279474929026378 1101 46-47 1.4170170524779166 1101 48-49 1.164049733069099 1101 50-51 1.378910633435602 1101 52-53 1.2994035400399824 1101 54-55 1.5373536735482034 1101 56-57 1.6752857618382997 1101 58-59 1.6905475838297797 1101 60-61 1.7213583333389408 1101 62-63 1.6077287447561766 1101 64-65 1.6913110637589277 1101 66-67 1.75969692256567 1101 68-69 1.9949239559350822 1101 70-71 2.3534340735854933 1101 72-73 2.492488043153731 1101 74-75 2.409468540795583 1101 76-77 2.8221090550457895 1101 78-79 2.9127494756989165 1101 80-81 2.963131189298746 1101 82-83 3.0572394620217977 1101 84-85 3.242793435978019 1101 86-87 3.500961627642969 1101 88-89 3.71660774059821 1101 90-91 3.497667235039014 1101 92-93 3.4426743947429 1101 94-95 3.5016484782818136 1101 96-97 3.456122194398233 1101 98-99 3.3319904673385228 1101 100-101 2.5647716515484387 1102 1 0.5515824683401469 1102 2 0.46320971017479096 1102 3 0.43738974984780654 1102 4 0.2969293380463185 1102 5 0.3116232844517839 1102 6 0.47920839628022094 1102 7 0.47056140289453907 1102 8 0.41585342613385023 1102 9 0.4861715443675081 1102 10-11 0.5330915218521639 1102 12-13 0.5708221749014228 1102 14-15 0.6533025961901373 1102 16-17 0.8483040932106363 1102 18-19 0.8232026193986712 1102 20-21 0.698112822657265 1102 22-23 0.6621659588961251 1102 24-25 0.779064060507153 1102 26-27 0.7244127860976519 1102 28-29 0.7121391082040986 1102 30-31 0.5761027212465919 1102 32-33 0.5596550554187445 1102 34-35 0.5654728521521974 1102 36-37 0.799772457315072 1102 38-39 0.9117770591117988 1102 40-41 1.1120404132639479 1102 42-43 1.169164724379435 1102 44-45 1.140917528539461 1102 46-47 1.1028591736015727 1102 48-49 1.217506405298593 1102 50-51 1.2427635579043113 1102 52-53 1.3391624745585489 1102 54-55 1.1974871932988478 1102 56-57 1.4001998277454923 1102 58-59 1.5896023264798735 1102 60-61 1.4869318481322793 1102 62-63 1.5729720002640555 1102 64-65 1.8458376328203911 1102 66-67 2.058741027271317 1102 68-69 2.0893143899372966 1102 70-71 1.9114387321333943 1102 72-73 2.0940010109611684 1102 74-75 2.110506370645492 1102 76-77 2.312277497412751 1102 78-79 2.6703848220423474 1102 80-81 3.036113025155853 1102 82-83 3.2779262703345218 1102 84-85 3.216148978229274 1102 86-87 3.4363053675484103 1102 88-89 3.5825931429351705 1102 90-91 3.495220272201607 1102 92-93 3.7975530408895235 1102 94-95 3.622557434677079 1102 96-97 3.30952454596121 1102 98-99 3.0391523014568005 1102 100-101 2.0981721464779195 1103 1 0.5466409447529266 1103 2 0.49065033290978377 1103 3 0.4882664954063891 1103 4 0.39119419455326465 1103 5 0.5218309131477383 1103 6 0.4172277026766622 1103 7 0.5007235837673463 1103 8 0.43297305244157513 1103 9 0.631397164925346 1103 10-11 0.5546376864652203 1103 12-13 0.5422264695970824 1103 14-15 0.6504745841042947 1103 16-17 0.7395534871099372 1103 18-19 0.8556523010212373 1103 20-21 0.7523726288973407 1103 22-23 0.7421556843731381 1103 24-25 0.8334213922996128 1103 26-27 0.9103604757722508 1103 28-29 0.8435748216950856 1103 30-31 0.7913158603651951 1103 32-33 1.112847460513322 1103 34-35 1.0780399124161164 1103 36-37 1.0729259620766527 1103 38-39 1.1268447111681041 1103 40-41 1.40788669467873 1103 42-43 1.2921682549875726 1103 44-45 1.5820824918666432 1103 46-47 1.6994503350393302 1103 48-49 1.632423030108633 1103 50-51 1.752403249064237 1103 52-53 1.7681795543504748 1103 54-55 1.895082661886235 1103 56-57 2.058362808053115 1103 58-59 2.1471417155853025 1103 60-61 2.1823002543403973 1103 62-63 2.3415853013757975 1103 64-65 2.4188580587002875 1103 66-67 2.5579738720458955 1103 68-69 2.6890755896938963 1103 70-71 2.733412017457905 1103 72-73 3.071122798398502 1103 74-75 2.68050258866867 1103 76-77 2.7818560496665263 1103 78-79 3.106611695780643 1103 80-81 3.2704597161321765 1103 82-83 3.479826370286766 1103 84-85 3.555980313967627 1103 86-87 3.45452765071774 1103 88-89 3.7192509376763887 1103 90-91 3.7581448116909826 1103 92-93 3.7073147763498593 1103 94-95 3.415070562852671 1103 96-97 3.425344495476857 1103 98-99 3.420020718424782 1103 100-101 2.42783655784711 1104 1 0.5240906663116256 1104 2 0.46446800347152006 1104 3 0.5881908285829986 1104 4 0.3453510985476953 1104 5 0.37251012124802685 1104 6 0.38689195804752075 1104 7 0.47867271505526787 1104 8 0.5720828445458324 1104 9 0.6367126451488332 1104 10-11 0.5732581157250038 1104 12-13 0.6870860490582302 1104 14-15 0.7272403262706177 1104 16-17 0.7933291550853951 1104 18-19 0.7406336212497138 1104 20-21 0.8213129086233053 1104 22-23 0.8385939311374102 1104 24-25 0.824948436178758 1104 26-27 0.994434554918314 1104 28-29 1.0643104440748132 1104 30-31 0.9110687161302238 1104 32-33 0.9696264231800882 1104 34-35 0.9196668165040194 1104 36-37 1.1078792631912648 1104 38-39 1.0943353115053327 1104 40-41 1.112633162998769 1104 42-43 1.1659548632423196 1104 44-45 1.136601894505219 1104 46-47 1.2988530866510786 1104 48-49 1.3204680741490762 1104 50-51 1.375397377223102 1104 52-53 1.602523608201082 1104 54-55 1.6348238404845006 1104 56-57 1.8984319776591754 1104 58-59 1.9961523843682976 1104 60-61 2.0323621289777947 1104 62-63 2.0761679959102928 1104 64-65 1.9786378380296554 1104 66-67 2.292823443749068 1104 68-69 2.5051491271515793 1104 70-71 2.360762096178785 1104 72-73 2.7436027247358403 1104 74-75 2.74119902124205 1104 76-77 2.8418976522902817 1104 78-79 2.9544263353220224 1104 80-81 3.101409088049717 1104 82-83 3.3375236164795687 1104 84-85 3.61729842742265 1104 86-87 3.6041869978528247 1104 88-89 3.6700479934305115 1104 90-91 3.6111080449184776 1104 92-93 3.559700944080669 1104 94-95 3.6635178279024014 1104 96-97 3.548841185243532 1104 98-99 2.996524582953997 1104 100-101 2.1870690080240607 1105 1 0.45427010790824696 1105 2 0.41419443989603266 1105 3 0.3040878508953888 1105 4 0.23242125122132506 1105 5 0.3521021373025235 1105 6 0.36374389271025365 1105 7 0.30749738802636983 1105 8 0.3981826035923106 1105 9 0.37164379269324144 1105 10-11 0.42679012195726074 1105 12-13 0.4160250533506229 1105 14-15 0.5814492199503363 1105 16-17 0.4302658078973636 1105 18-19 0.5371792044571322 1105 20-21 0.5319290372894514 1105 22-23 0.47460824504226906 1105 24-25 0.5798441546119903 1105 26-27 0.3957389570925969 1105 28-29 0.3761893951346309 1105 30-31 0.4657897471832655 1105 32-33 0.5364212287933015 1105 34-35 0.6440132686503972 1105 36-37 0.727269039376985 1105 38-39 0.6897038992624687 1105 40-41 0.7907317588574188 1105 42-43 0.7812631786231279 1105 44-45 0.8082821412851473 1105 46-47 0.9766883438991698 1105 48-49 0.942892128287518 1105 50-51 0.8550002720126599 1105 52-53 0.9604700272928426 1105 54-55 1.0823210776519048 1105 56-57 1.282096929356026 1105 58-59 1.184703300247957 1105 60-61 1.385565994903665 1105 62-63 1.4135736895682491 1105 64-65 1.6127124420933896 1105 66-67 1.6877011808884923 1105 68-69 1.8961606769163026 1105 70-71 1.9188164657953166 1105 72-73 2.107375752481321 1105 74-75 2.2730305661218004 1105 76-77 2.375036789742577 1105 78-79 2.7698533753594283 1105 80-81 2.7866150021066645 1105 82-83 3.145821999261461 1105 84-85 3.23198320299905 1105 86-87 3.183630948047046 1105 88-89 3.3163687058621356 1105 90-91 3.416174188150624 1105 92-93 3.403030004372395 1105 94-95 3.431310495948784 1105 96-97 3.28036598117572 1105 98-99 2.9620887316642133 1105 100-101 2.0003949354493002 1106 1 0.18502097093694303 1106 2 0.2202630393252072 1106 3 0.10144769227974493 1106 4 0.17759640293376577 1106 5 0.17763217417066102 1106 6 -0.13482077041008012 1106 7 0.14253671029756276 1106 8 -0.10994155008852857 1106 9 0.3146674697312193 1106 10-11 0.2616437769845632 1106 12-13 0.4137241877198221 1106 14-15 0.45735844861748376 1106 16-17 -0.17437005674872097 1106 18-19 0.37067757534059353 1106 20-21 0.34552468576763573 1106 22-23 0.3765490519371113 1106 24-25 0.4162203932864941 1106 26-27 0.3000391002589353 1106 28-29 0.4594307196936285 1106 30-31 0.23172167021426304 1106 32-33 0.2762688683855856 1106 34-35 0.23173402593192804 1106 36-37 0.3440088878839376 1106 38-39 0.4796080706783883 1106 40-41 0.6216358904765684 1106 42-43 0.24752342501616198 1106 44-45 0.6802347035339267 1106 46-47 0.8315873774498925 1106 48-49 0.6214715672346856 1106 50-51 0.5085385110973775 1106 52-53 0.7023932725868747 1106 54-55 0.7188777793771521 1106 56-57 1.297696447304883 1106 58-59 1.3056162994040612 1106 60-61 1.5226376566433544 1106 62-63 1.365195765143337 1106 64-65 1.2721020310539686 1106 66-67 1.5241862484256536 1106 68-69 1.6336772461489097 1106 70-71 1.453548179434076 1106 72-73 1.4282628837361706 1106 74-75 1.855923413825682 1106 76-77 2.0963448994061515 1106 78-79 2.1967958914745687 1106 80-81 2.0886535151768406 1106 82-83 2.3312244415586925 1106 84-85 2.5153381524418457 1106 86-87 2.090394664412397 1106 88-89 2.0710835007710067 1106 90-91 1.9074273170442062 1106 92-93 1.887243798749621 1106 94-95 1.9053633072845368 1106 96-97 1.8363724874375897 1106 98-99 1.9324396779600015 1106 100-101 1.5072913077185017 1107 1 0.14364597886857666 1107 2 0.07617648614774453 1107 3 0.07958830986566312 1107 4 0.043651765137042275 1107 5 0.11819919717220273 1107 6 0.02592121544746817 1107 7 0.09282222915010152 1107 8 0.08882857780331932 1107 9 0.22436058435499717 1107 10-11 0.10262919115950098 1107 12-13 0.16211241992932202 1107 14-15 0.2211211796375281 1107 16-17 0.10211042504763412 1107 18-19 0.15790088105387667 1107 20-21 0.2727417219985355 1107 22-23 0.2873718506643783 1107 24-25 0.34396859954009074 1107 26-27 0.3226257985004892 1107 28-29 0.4201699319736747 1107 30-31 0.4098053995306614 1107 32-33 0.5652601801041612 1107 34-35 0.6029802681334928 1107 36-37 0.6931552046727845 1107 38-39 0.7073641786348048 1107 40-41 0.467657343200635 1107 42-43 0.6749936749055152 1107 44-45 0.6854823520789566 1107 46-47 0.7614915912524083 1107 48-49 0.7539614736074824 1107 50-51 0.930387776937394 1107 52-53 0.9871333285133659 1107 54-55 1.0785457322654466 1107 56-57 1.138903947218079 1107 58-59 1.1413721232184422 1107 60-61 1.2723858397505552 1107 62-63 1.526669456663253 1107 64-65 1.6504401513027176 1107 66-67 1.905228706509714 1107 68-69 1.8953215825236 1107 70-71 2.0339055040132834 1107 72-73 2.179964244066195 1107 74-75 2.1908610322763984 1107 76-77 2.3182734017660884 1107 78-79 2.6254255566219555 1107 80-81 2.723381040120085 1107 82-83 2.8027545664016067 1107 84-85 2.82479208092505 1107 86-87 2.7906736982815694 1107 88-89 3.112853577980218 1107 90-91 3.0010844445996376 1107 92-93 2.815326729914217 1107 94-95 2.9315715195482195 1107 96-97 2.8747805497285484 1107 98-99 2.590401202254345 1107 100-101 1.8446927210058917 1108 1 0.3162542476078585 1108 2 0.3011311370168386 1108 3 0.238990164461228 1108 4 0.18581238804204503 1108 5 0.19787181166780954 1108 6 0.2000359976879693 1108 7 0.14477456712595682 1108 8 0.24051203172389535 1108 9 0.3111226855969065 1108 10-11 0.30465650454894444 1108 12-13 0.22129697620057698 1108 14-15 0.3179276123061854 1108 16-17 0.3876986444311825 1108 18-19 0.33198783696646217 1108 20-21 0.36884364263784875 1108 22-23 0.3495071652322608 1108 24-25 0.4370237318630359 1108 26-27 0.43733681916629763 1108 28-29 0.3783179672597896 1108 30-31 0.42166262595018367 1108 32-33 0.44627898564250046 1108 34-35 0.556929641340588 1108 36-37 0.6567058285468406 1108 38-39 0.6340599987311677 1108 40-41 0.7607706383866102 1108 42-43 0.6516897090505651 1108 44-45 0.6279836237226846 1108 46-47 0.8619189491639787 1108 48-49 0.9310579539078034 1108 50-51 1.031794407542698 1108 52-53 1.033566129488186 1108 54-55 1.1376019566258222 1108 56-57 1.330918452713103 1108 58-59 1.4331538410485756 1108 60-61 1.6081601063516757 1108 62-63 1.5440272475875112 1108 64-65 1.6610575494126323 1108 66-67 1.8614468010951022 1108 68-69 2.094990857859859 1108 70-71 2.149777836042478 1108 72-73 2.116682309657712 1108 74-75 2.2103011276409212 1108 76-77 2.261449802393603 1108 78-79 2.6195262778103228 1108 80-81 2.7495288372284037 1108 82-83 2.77576565529057 1108 84-85 2.7769129919629165 1108 86-87 2.8871020431521295 1108 88-89 3.1931104480670705 1108 90-91 3.2333055615523314 1108 92-93 3.0278961351974853 1108 94-95 2.7410727705654363 1108 96-97 2.636042720828163 1108 98-99 2.195313463701959 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88-89 0.309858099118447 2316 90-91 0.19681910734149355 2316 92-93 0.440181962749838 2316 94-95 0.5111179112880109 2316 96-97 0.36688679906065147 2316 98-99 0.09920432738547014 2316 100-101 0.33191068211786146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8405.0 3 3284.0 4 3473.0 5 4204.0 6 5141.0 7 6625.0 8 8479.0 9 11049.0 10 14106.0 11 17838.0 12 21705.0 13 25687.0 14 29686.0 15 33461.0 16 38701.0 17 44334.0 18 45664.0 19 47350.0 20 52034.0 21 58201.0 22 64603.0 23 72525.0 24 80641.0 25 89049.0 26 98223.0 27 108945.0 28 120230.0 29 134192.0 30 149781.0 31 167569.0 32 188443.0 33 212709.0 34 240166.0 35 262779.0 36 261391.0 37 184111.0 38 44747.0 39 573.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8846434677093 17.87105834602551 13.906215927400414 24.33808225886477 2 15.711826616933747 19.247693860048106 39.883971541668664 25.15650798134948 3 19.726687907301795 22.682274523268497 28.919458161040424 28.671579408389285 4 13.136111633088147 15.646997670705643 36.0154804118917 35.201410284314505 5 14.30943469701231 36.77018205222508 33.962326655561085 14.958056595201525 6 34.115051965101514 34.78825928064129 16.867329790125076 14.229358964132114 7 30.260190132443842 31.462086725538107 19.73555165782919 18.542171484188863 8 27.04457078123405 34.73950415926817 19.23459597273883 18.981329086758947 9 26.60761103852697 16.574924518877545 19.4015379426553 37.415926499940184 10-11 25.4994520095181 25.805113428791483 27.121743782428688 21.573690779261728 12-13 26.39776036636508 22.921742586088158 27.26502872027018 23.415468327276585 14-15 22.868902454478025 24.749103246223328 25.236400008785907 27.145594290512744 16-17 23.064507899564347 27.816681179415077 26.108366810645293 23.010444110375282 18-19 23.133990317739205 26.694915182645257 27.74143602461301 22.42965847500253 20-21 23.385137682235683 25.87466622193903 28.020764319866636 22.71943177595865 22-23 23.19995539236973 25.772869436507627 27.849692562184558 23.177482608938085 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26.424495985522068 27.011023342156783 23.384646522531558 50-51 23.273057768587783 26.695503659105192 26.968433038037126 23.0630055342699 52-53 23.449363886425797 26.412172810446076 26.987192925760933 23.15127037736719 54-55 22.941689015741993 26.546968195052866 27.218099271112695 23.293243518092446 56-57 23.202810965128997 26.601156313480047 26.93296506987292 23.263067651518032 58-59 23.203588262639258 26.281510305461108 26.94614227644474 23.568759155454895 60-61 22.896327089373443 26.453659506459758 27.323823892053127 23.326189512113668 62-63 22.952461107860056 26.45890292473217 27.404970868905345 23.18366509850243 64-65 22.644816571026045 26.49460363901732 27.260432485941084 23.600147304015554 66-67 22.709142282140622 26.46253524295787 27.13096689395922 23.697355580942293 68-69 23.077950908924443 26.10694452937522 27.29281837018278 23.522286191517562 70-71 22.966233892604865 26.184384928015707 27.030725302838793 23.81865587654063 72-73 22.90193981045 26.222992469591265 27.07975658486326 23.795311135095467 74-75 22.856472923859624 26.592625202590863 26.875382418238093 23.67551945531142 76-77 23.000109292183616 26.138247686493937 26.985050957691765 23.876592063630685 78-79 23.02110353274067 26.170492218679392 27.012994910995207 23.79540933758473 80-81 23.182878240252318 26.24469865199422 26.78305629628253 23.78936681147093 82-83 23.02069793662598 26.275810758440056 26.882382376125424 23.821108928808545 84-85 23.059251852681477 26.16133722477107 26.87595560760807 23.903455314939382 86-87 23.15748222359045 26.220252649253005 26.656143133563564 23.966121993592974 88-89 23.016670512833077 26.22033566727496 26.594889205066668 24.1681046148253 90-91 23.07791081280448 26.09363611389042 26.602561828172274 24.225891245132832 92-93 23.161078800434893 25.715418510537514 26.830001696057103 24.293500992970486 94-95 22.949754478559427 25.58754932180668 26.907984156201138 24.554712043432755 96-97 23.04471595535838 25.353852079862953 26.88103339336458 24.72039857141409 98-99 22.957208040448883 25.453538010362237 26.443728176201986 25.145525772986886 100-101 23.879031497013806 24.43475072545045 26.648850187675105 25.037367589860636 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 184.0 1 140.5 2 131.5 3 171.5 4 148.5 5 138.5 6 250.0 7 530.0 8 900.0 9 1055.0 10 940.5 11 863.0 12 911.5 13 989.0 14 1135.5 15 1324.0 16 1498.0 17 1785.5 18 2023.5 19 2271.5 20 2672.5 21 3195.5 22 3887.0 23 4811.5 24 6036.5 25 7720.0 26 9939.0 27 12961.5 28 16534.0 29 20345.0 30 24853.0 31 31090.0 32 39313.5 33 44478.5 34 47190.5 35 51623.5 36 57279.0 37 63057.5 38 68799.5 39 74373.5 40 80121.0 41 87458.5 42 93399.0 43 98247.0 44 103384.5 45 109793.0 46 114845.0 47 118359.5 48 121734.0 49 121808.5 50 120126.0 51 117152.0 52 114265.5 53 113000.0 54 111370.0 55 105410.5 56 96688.0 57 89049.5 58 82035.0 59 71763.0 60 59022.5 61 49324.5 62 41516.0 63 32497.0 64 24456.0 65 18872.5 66 14103.0 67 10591.5 68 8108.5 69 6062.5 70 4635.0 71 3531.5 72 2621.5 73 1998.0 74 1559.0 75 1208.0 76 887.5 77 601.0 78 389.0 79 237.0 80 134.5 81 78.0 82 56.5 83 38.5 84 24.5 85 24.0 86 22.0 87 11.5 88 5.5 89 5.5 90 3.0 91 0.5 92 1.0 93 1.5 94 3.0 95 2.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.386371559918165 2 0.028039555366973595 3 0.03364746644036831 4 0.042836332777497 5 0.06188971738830798 6 0.05337650298773287 7 0.037633812866034434 8 0.039525638288384464 9 0.036687900154859426 10-11 0.0375324650755514 12-13 0.029390859240080756 14-15 0.027667946801869125 16-17 0.021519514179231543 18-19 0.026164621243037405 20-21 0.0334278795609884 22-23 0.03273533632602098 24-25 0.04074181177418091 26-27 0.035269031088096904 28-29 0.03048879363698032 30-31 0.03332653177050536 32-33 0.027971990173318237 34-35 0.029830032998840583 36-37 0.031890771405329005 38-39 0.014256255861280551 40-41 0.01217862615637829 42-43 0.012533343423068917 44-45 0.012415104334172042 46-47 0.02124925340461011 48-49 0.01912094980446633 50-51 0.012296865245275165 52-53 0.009948974765751474 54-55 0.008445649206919757 56-57 0.00900306205457646 58-59 0.004053911619321483 60-61 0.006874758454432682 62-63 0.01283738679451803 64-65 0.008074040641815288 66-67 0.003901889933596928 68-69 0.0059795196384991875 70-71 0.012195517454792129 72-73 0.010286800734028264 74-75 0.010844213581684968 76-77 0.005016715628910336 78-79 0.009847626975268437 80-81 0.012212408753205969 82-83 0.014762994813695736 84-85 0.009205757635542534 86-87 0.00241545567317905 88-89 0.015100820781972524 90-91 0.024762643474688727 92-93 0.01006721385464835 94-95 0.01981349303943375 96-97 0.018039906705980602 98-99 0.01077664838802961 100-101 0.12381321737344364 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2960104.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.20225083699049 #Duplication Level Percentage of deduplicated Percentage of total 1 76.8079189928016 43.935858484934826 2 12.889348679426071 14.745995125719244 3 4.425673284006681 7.594754199429526 4 1.9969944396168022 4.5693030742014225 5 1.1030128198726619 3.15474079993861 6 0.6455544710363686 2.215630126869784 7 0.43679634243302246 1.749001376117362 8 0.28902562456266934 1.3226333019641319 9 0.232824673852547 1.1986285855278502 >10 1.0815099383822757 10.840675782256737 >50 0.057544413591143706 2.258154198533414 >100 0.02934440283416736 3.1830272259140666 >500 0.00225563824282053 0.9035665927846446 >1k 0.0021369204400007425 2.149735376765648 >5k 5.935890111113175E-5 0.1782957490427789 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5251 0.17739241594214258 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3826 0.12925221546269994 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3193 0.10786783167077912 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3182 0.10749622310567467 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3116 0.10526657171504784 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.378259682767903E-5 0.0 4 0.0 0.0 0.0 3.378259682767903E-5 0.0 5 0.0 0.0 0.0 6.756519365535806E-5 0.0 6 0.0 0.0 0.0 6.756519365535806E-5 0.0 7 0.0 0.0 0.0 6.756519365535806E-5 0.0 8 0.0 0.0 0.0 6.756519365535806E-5 0.0 9 0.0 0.0 0.0 1.0134779048303708E-4 0.0 10-11 0.0 0.0 0.0 1.8580428255223465E-4 0.0 12-13 0.0 0.0 0.0 2.364781777937532E-4 0.0 14-15 0.0 0.0 0.0 2.7026077462143224E-4 0.0 16-17 0.0 0.0 0.0 2.7026077462143224E-4 0.0 18-19 0.0 0.0 0.0 2.871520730352718E-4 3.378259682767903E-5 20-21 0.0 0.0 0.0 3.884998635183088E-4 3.378259682767903E-5 22-23 0.0 0.0 0.0 4.391737587598274E-4 3.378259682767903E-5 24-25 0.0 0.0 0.0 7.601084286227781E-4 3.378259682767903E-5 26-27 0.0 0.0 0.0 0.0011148256953134078 3.378259682767903E-5 28-29 0.0 0.0 0.0 0.003310694489112545 3.378259682767903E-5 30-31 0.0 0.0 0.0 0.01179012629285998 3.378259682767903E-5 32-33 0.0 0.0 0.0 0.028884120287665568 3.378259682767903E-5 34-35 0.0 0.0 0.0 0.057261501622915954 3.378259682767903E-5 36-37 0.0 0.0 0.0 0.10459091977849427 3.378259682767903E-5 38-39 0.0 0.0 0.0 0.18041595835821983 3.378259682767903E-5 40-41 0.0 0.0 0.0 0.2591800828619535 3.378259682767903E-5 42-43 0.0 0.0 0.0 0.34122111925797205 3.378259682767903E-5 44-45 0.0 0.0 0.0 0.44484923502687745 3.378259682767903E-5 46-47 0.0 0.0 0.0 0.5694563434257716 3.378259682767903E-5 48-49 1.6891298413839515E-5 0.0 0.0 0.6987085588884714 3.378259682767903E-5 50-51 3.378259682767903E-5 0.0 0.0 0.8406123568631372 3.378259682767903E-5 52-53 3.378259682767903E-5 0.0 0.0 0.9831411328791151 3.378259682767903E-5 54-55 3.378259682767903E-5 1.6891298413839515E-5 0.0 1.1379161002451266 3.378259682767903E-5 56-57 3.378259682767903E-5 3.378259682767903E-5 0.0 1.3224028615210817 3.378259682767903E-5 58-59 3.378259682767903E-5 3.378259682767903E-5 0.0 1.5211289873599037 3.378259682767903E-5 60-61 6.756519365535806E-5 3.378259682767903E-5 0.0 1.733368151929797 3.378259682767903E-5 62-63 6.756519365535806E-5 3.378259682767903E-5 0.0 1.9400669706199511 3.378259682767903E-5 64-65 6.756519365535806E-5 3.378259682767903E-5 0.0 2.161782153600009 3.378259682767903E-5 66-67 6.756519365535806E-5 3.378259682767903E-5 0.0 2.4172630421093313 3.378259682767903E-5 68-69 6.756519365535806E-5 3.378259682767903E-5 0.0 2.7045164629350857 3.378259682767903E-5 70-71 1.0134779048303708E-4 3.378259682767903E-5 0.0 2.9986446422152735 3.378259682767903E-5 72-73 1.0134779048303708E-4 3.378259682767903E-5 0.0 3.3052723823217023 3.378259682767903E-5 74-75 1.0134779048303708E-4 3.378259682767903E-5 0.0 3.6110217749106113 3.378259682767903E-5 76-77 1.182390888968766E-4 3.378259682767903E-5 0.0 3.943763462364836 3.378259682767903E-5 78-79 1.3513038731071612E-4 5.0673895241518545E-5 0.0 4.3112167680595 3.378259682767903E-5 80-81 1.3513038731071612E-4 6.756519365535806E-5 0.0 4.700831457273123 3.378259682767903E-5 82-83 1.3513038731071612E-4 6.756519365535806E-5 0.0 5.0976418396110414 3.378259682767903E-5 84-85 1.3513038731071612E-4 6.756519365535806E-5 0.0 5.503252588422569 3.378259682767903E-5 86-87 1.3513038731071612E-4 6.756519365535806E-5 0.0 5.9219878761016504 3.378259682767903E-5 88-89 1.3513038731071612E-4 6.756519365535806E-5 0.0 6.361330547845617 3.378259682767903E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5680 0.0 33.76166 1 GTATCAA 8820 0.0 33.640923 1 ATCAACG 10530 0.0 28.028397 3 TCAACGC 10635 0.0 27.885735 4 TATCAAC 10685 0.0 27.621807 2 CAACGCA 10720 0.0 27.619825 5 AACGCAG 11175 0.0 26.659964 6 GTACATG 9545 0.0 26.587763 1 TACATGG 9555 0.0 25.914452 2 TAAGGTG 1625 0.0 23.982153 5 GTAAGGT 1450 0.0 23.599321 4 ACATGGG 9940 0.0 23.57195 3 ACGCAGA 12590 0.0 23.355385 7 CGCAGAG 12715 0.0 23.0137 8 GCAGAGT 13955 0.0 20.524862 9 AGGTAAG 1700 0.0 20.128832 2 GTGTAGC 1975 0.0 20.047356 1 AGAGTAC 10885 0.0 20.02965 10-11 CATGGGG 6420 0.0 19.987743 4 AAGGTAA 1695 0.0 19.981808 1 >>END_MODULE