FastQCFastQC Report
Thu 26 May 2016
SRR938584_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938584_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2960104
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51130.17273041757992288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41830.14131260253018138No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA32420.10952317891533542No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA32300.10911778775340326No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57250.033.4490131
GTATCAA84450.032.4098171
ATCAACG101900.026.6624153
TCAACGC102300.026.6041434
TATCAAC106050.025.8917142
CAACGCA105900.025.6549025
GTACATG90000.025.078691
AACGCAG109200.024.9231136
TACATGG90400.024.435892
ACATGGG93600.022.8864443
ACGCAGA123200.021.8981747
CGCAGAG126550.021.2434278
TAAGGTG15250.020.2448145
CATGGGG61250.019.8519954
AGAGTAC107050.019.76623710-11
GAGTACT67750.019.66466312-13
GTAAGGT15500.019.3054164
CTATACG1759.947601E-518.998984
AGTACTT71300.018.95202812-13
GTACTTT70800.018.8510714-15
Produced by FastQC (version 0.11.5)