##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938584_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2960104 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.819372562585638 31.0 31.0 34.0 30.0 34.0 2 32.076097326310155 33.0 31.0 34.0 30.0 34.0 3 32.13950286881812 33.0 31.0 34.0 30.0 34.0 4 35.78882431157824 37.0 35.0 37.0 35.0 37.0 5 35.68090715731609 37.0 35.0 37.0 33.0 37.0 6 35.70824268336518 37.0 35.0 37.0 33.0 37.0 7 35.6296292292433 37.0 35.0 37.0 33.0 37.0 8 35.59770974263066 37.0 35.0 37.0 33.0 37.0 9 37.14942076359479 39.0 37.0 39.0 33.0 39.0 10-11 37.07524516030517 39.0 37.0 39.0 33.0 39.0 12-13 36.96039801304279 39.0 37.0 39.0 33.0 39.0 14-15 37.995048653695946 40.0 37.0 41.0 33.0 41.0 16-17 38.08175675584371 40.0 37.0 41.0 33.0 41.0 18-19 38.20085713204671 40.0 37.5 41.0 33.0 41.0 20-21 38.14303196779573 40.0 37.0 41.0 33.0 41.0 22-23 38.02166123217292 40.0 37.0 41.0 33.0 41.0 24-25 37.90408428217387 40.0 37.0 41.0 32.5 41.0 26-27 37.69470819268513 40.0 37.0 41.0 32.0 41.0 28-29 37.561812017415605 39.5 37.0 41.0 32.0 41.0 30-31 37.319964771508026 39.0 36.0 41.0 31.0 41.0 32-33 37.24962805360893 39.0 36.0 41.0 31.0 41.0 34-35 37.13360848132363 39.0 36.0 41.0 31.0 41.0 36-37 37.023018110174505 39.0 36.0 41.0 31.0 41.0 38-39 36.8232545207871 39.0 35.5 41.0 30.0 41.0 40-41 36.61203052325189 39.0 35.0 40.0 30.0 41.0 42-43 36.364826370965346 39.0 35.0 40.0 30.0 41.0 44-45 36.106449807168936 38.0 35.0 40.0 29.5 41.0 46-47 35.73850496469043 38.0 35.0 40.0 28.5 41.0 48-49 35.35792847143209 38.0 34.0 40.0 27.0 41.0 50-51 34.977768179766656 38.0 34.0 40.0 26.5 41.0 52-53 34.38214518814203 38.0 33.0 40.0 24.5 41.0 54-55 33.95427272149898 37.0 33.0 40.0 24.0 41.0 56-57 33.56200356473962 37.0 32.0 39.5 23.0 40.5 58-59 33.01784734590406 36.0 31.5 39.0 21.5 40.0 60-61 32.5107141505839 36.0 31.0 39.0 20.0 40.0 62-63 31.993470330772162 35.0 30.5 38.5 18.0 40.0 64-65 31.333702633420987 35.0 29.5 38.0 14.0 40.0 66-67 30.676794970717246 34.0 29.0 38.0 9.5 40.0 68-69 29.93199377454306 34.0 28.0 37.0 7.5 39.0 70-71 29.153343429825437 33.5 26.5 36.0 4.5 39.0 72-73 28.56284576487853 33.0 26.0 36.0 2.0 38.5 74-75 27.845105273328237 32.5 26.0 35.0 2.0 38.0 76-77 26.710015594046695 31.0 24.5 34.0 2.0 36.0 78-79 27.210995120441716 32.0 26.0 35.0 2.0 36.0 80-81 26.836946945107336 32.0 25.0 35.0 2.0 36.0 82-83 26.146772039090518 31.5 23.5 34.5 2.0 36.0 84-85 25.37268622994327 31.0 20.5 34.0 2.0 35.0 86-87 24.46285789283079 31.0 17.5 34.0 2.0 35.0 88-89 23.48005492374592 30.0 9.0 34.0 2.0 35.0 90-91 22.617366484420817 29.5 2.0 34.0 2.0 35.0 92-93 21.54043320775216 29.0 2.0 34.0 2.0 35.0 94-95 20.357028334139613 27.5 2.0 33.0 2.0 34.5 96-97 18.969631472407727 25.5 2.0 33.0 2.0 34.0 98-99 17.18450247018348 21.0 2.0 32.0 2.0 34.0 100-101 12.897936863029138 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29554941587985084 1101 2 0.30802095699440457 1101 3 0.25508006545062756 1101 4 0.16593068327848925 1101 5 0.16735899400009657 1101 6 0.1889931126136304 1101 7 0.2616887130427017 1101 8 0.2648184748932323 1101 9 0.32245760183200645 1101 10-11 0.32447960019035094 1101 12-13 0.3577189543961623 1101 14-15 0.4508361473637805 1101 16-17 0.35566473858656167 1101 18-19 0.38409850742806384 1101 20-21 0.43083386111680255 1101 22-23 0.391385645538584 1101 24-25 0.3588270109581302 1101 26-27 0.5110274449433518 1101 28-29 0.4604319715461074 1101 30-31 0.5932712623412897 1101 32-33 0.4381995164873942 1101 34-35 0.282760380122987 1101 36-37 0.3318678853193262 1101 38-39 0.41840793731767434 1101 40-41 0.4851836871484281 1101 42-43 0.5939292482511149 1101 44-45 0.6804874537071584 1101 46-47 0.6316106182321803 1101 48-49 0.7037034593603835 1101 50-51 0.7879531751419293 1101 52-53 0.7348133382752522 1101 54-55 0.7980989511867662 1101 56-57 0.9540599203995228 1101 58-59 0.9776065127705564 1101 60-61 1.0120544286740696 1101 62-63 1.0545958347351423 1101 64-65 1.1338321744789361 1101 66-67 1.328161567519306 1101 68-69 1.598442055794127 1101 70-71 1.7315880804648813 1101 72-73 1.7502598139086771 1101 74-75 1.6079594435632174 1101 76-77 1.7400743049681573 1101 78-79 1.8802618488185026 1101 80-81 1.9756962935784017 1101 82-83 2.1452360101660126 1101 84-85 2.267756378185595 1101 86-87 2.221990982504394 1101 88-89 2.147632436621265 1101 90-91 2.3926336710034093 1101 92-93 2.6891909110624965 1101 94-95 2.5129888162549108 1101 96-97 2.536764041550086 1101 98-99 2.4601657039052824 1101 100-101 2.370472948759984 1102 1 0.2752642812703847 1102 2 0.24199972964186145 1102 3 0.16160269222888957 1102 4 0.10623426170393202 1102 5 0.12348648534660356 1102 6 0.1828504359842853 1102 7 0.1773056631640486 1102 8 0.24358805937252725 1102 9 0.2032172005447066 1102 10-11 0.28710954980913783 1102 12-13 0.3600138287918426 1102 14-15 0.41945509477982057 1102 16-17 0.3278893954804616 1102 18-19 0.2842068542443741 1102 20-21 0.28272952396911677 1102 22-23 0.37013885488743625 1102 24-25 0.3179004353937316 1102 26-27 0.27090936493799944 1102 28-29 0.32675842426168344 1102 30-31 0.2094642989761084 1102 32-33 0.17972962752491384 1102 34-35 0.2064347190683975 1102 36-37 0.3743003838508656 1102 38-39 0.5245395165564659 1102 40-41 0.5186108652940646 1102 42-43 0.4856779142670149 1102 44-45 0.5304266418918004 1102 46-47 0.3924710995290681 1102 48-49 0.5441107901410831 1102 50-51 0.7101343731464738 1102 52-53 0.6908487636905463 1102 54-55 0.7252636079534298 1102 56-57 0.8161038641812226 1102 58-59 1.0064372860466264 1102 60-61 0.9737912857469908 1102 62-63 1.190313328157405 1102 64-65 1.2263415890008709 1102 66-67 1.298665952166644 1102 68-69 1.4069631788877537 1102 70-71 1.6170300240302922 1102 72-73 1.655459883838727 1102 74-75 1.5407374125805298 1102 76-77 1.6148563331329093 1102 78-79 1.5767158791150848 1102 80-81 1.8640818140990305 1102 82-83 1.9584739365702895 1102 84-85 1.8047919753630417 1102 86-87 1.7226358563463826 1102 88-89 1.6913642251789796 1102 90-91 1.8404879516627766 1102 92-93 2.212807501166594 1102 94-95 1.921594972220138 1102 96-97 2.0954679417291757 1102 98-99 2.459793199139412 1102 100-101 1.934229931457244 1103 1 0.23369985236491075 1103 2 0.3034186211197323 1103 3 0.26828534453930786 1103 4 0.19221265132355114 1103 5 0.17577071235129438 1103 6 0.19383648342016357 1103 7 0.2697482112274372 1103 8 0.2069260064733882 1103 9 0.31023111922671376 1103 10-11 0.256204824032217 1103 12-13 0.34168074194327147 1103 14-15 0.44557500175967846 1103 16-17 0.34593333967020357 1103 18-19 0.36603193735857786 1103 20-21 0.43051255754492246 1103 22-23 0.4815732451444106 1103 24-25 0.45619075293785016 1103 26-27 0.5395974127465806 1103 28-29 0.504094104007919 1103 30-31 0.4431222443553864 1103 32-33 0.48559551478080465 1103 34-35 0.5108781297521645 1103 36-37 0.5317029624630933 1103 38-39 0.4771907464086169 1103 40-41 0.6845091557581284 1103 42-43 0.6236914515462715 1103 44-45 0.7521810899498362 1103 46-47 0.745030435586095 1103 48-49 0.8015993102846863 1103 50-51 0.6777333398334804 1103 52-53 0.7461257202339837 1103 54-55 0.8996379220122606 1103 56-57 1.1110527273675288 1103 58-59 1.1202330080173013 1103 60-61 1.083802970498617 1103 62-63 1.240174869345008 1103 64-65 1.4225769653190135 1103 66-67 1.3497529728892204 1103 68-69 1.23156312952975 1103 70-71 1.5865297087892465 1103 72-73 1.4762455780764334 1103 74-75 1.575863094629831 1103 76-77 1.5572059114150214 1103 78-79 1.6033101357299984 1103 80-81 1.844345252135735 1103 82-83 1.9921012506845237 1103 84-85 2.092755399160101 1103 86-87 2.07813801449986 1103 88-89 1.9860002511180284 1103 90-91 2.209687298928376 1103 92-93 2.5384548212971936 1103 94-95 2.218345310926839 1103 96-97 2.4228110846984308 1103 98-99 2.543632780136676 1103 100-101 2.2907421429842127 1104 1 0.2276779165941143 1104 2 0.2499585214029878 1104 3 0.2116397824169738 1104 4 0.14148033950320382 1104 5 0.15245496215310794 1104 6 0.20386358740237398 1104 7 0.21405614672304552 1104 8 0.20616509950619388 1104 9 0.20848608707645866 1104 10-11 0.23965490729615624 1104 12-13 0.27649157814536096 1104 14-15 0.4331351347124297 1104 16-17 0.34516406392190646 1104 18-19 0.36922484471877937 1104 20-21 0.3696210888977518 1104 22-23 0.397755446333818 1104 24-25 0.3711872500051854 1104 26-27 0.44493514088553354 1104 28-29 0.3798337901373259 1104 30-31 0.38750240134502434 1104 32-33 0.522990652323351 1104 34-35 0.32758791194430614 1104 36-37 0.4728372279264903 1104 38-39 0.4741973855691697 1104 40-41 0.4786539869706772 1104 42-43 0.5597733643061886 1104 44-45 0.4503655644045139 1104 46-47 0.5001349029718725 1104 48-49 0.5468856215020494 1104 50-51 0.4868185103316378 1104 52-53 0.5150048752522807 1104 54-55 0.5562346082792615 1104 56-57 0.7609625401655009 1104 58-59 0.7746114782084632 1104 60-61 0.7328714490824737 1104 62-63 0.7756779559036424 1104 64-65 1.054588020370165 1104 66-67 0.9639599352368791 1104 68-69 1.1728201008874315 1104 70-71 1.2090206232274774 1104 72-73 1.3274734304233142 1104 74-75 1.1828829586382241 1104 76-77 1.2666019984338241 1104 78-79 1.4401702581837412 1104 80-81 1.7164403340965322 1104 82-83 1.665258667891738 1104 84-85 1.9802978924612695 1104 86-87 1.8979927194177293 1104 88-89 2.004677522878005 1104 90-91 2.1865047427722253 1104 92-93 2.2933694695116813 1104 94-95 2.2186977779022143 1104 96-97 2.415993027868538 1104 98-99 2.62835250531025 1104 100-101 2.2990829919177163 1105 1 0.13522896165874698 1105 2 0.19567970046957583 1105 3 0.19363568510878082 1105 4 0.14988405772932367 1105 5 0.14630376970252712 1105 6 0.06579842273337277 1105 7 0.12742679202555252 1105 8 0.10885795254337438 1105 9 0.165077528336262 1105 10-11 0.2069649883793474 1105 12-13 0.17499899477528658 1105 14-15 0.33839113279191935 1105 16-17 0.20852359513210672 1105 18-19 0.2222930402661305 1105 20-21 0.2583267129422566 1105 22-23 0.3096983622636884 1105 24-25 0.25255788693943515 1105 26-27 0.3550964024422285 1105 28-29 0.21709560206386413 1105 30-31 0.3831508027201451 1105 32-33 0.38037807179848215 1105 34-35 0.3248402468189724 1105 36-37 0.3827368245241587 1105 38-39 0.4381572869701742 1105 40-41 0.4316276735072506 1105 42-43 0.4474553412233959 1105 44-45 0.4914038141415631 1105 46-47 0.5633227802953016 1105 48-49 0.6257857395467852 1105 50-51 0.5467570977801941 1105 52-53 0.5315133033953643 1105 54-55 0.6323132636257682 1105 56-57 0.7457782319039907 1105 58-59 0.7447334507950956 1105 60-61 0.8626941413915645 1105 62-63 0.8727713461829687 1105 64-65 1.060519702960054 1105 66-67 1.0520957332313188 1105 68-69 1.1933607217180118 1105 70-71 1.2230552897811755 1105 72-73 1.357998742572402 1105 74-75 1.3062677410661365 1105 76-77 1.4077133658228256 1105 78-79 1.549713837716446 1105 80-81 1.5224353539371478 1105 82-83 1.6053047560712095 1105 84-85 1.9463083831183745 1105 86-87 2.058938269779734 1105 88-89 1.9196244864295338 1105 90-91 1.992977014811732 1105 92-93 2.063632708749182 1105 94-95 2.0128361271692334 1105 96-97 2.2907798857289734 1105 98-99 2.385922771216869 1105 100-101 2.4689144290561487 1106 1 0.011431308851900468 1106 2 0.08109642878722667 1106 3 0.022535794192748426 1106 4 0.08881632056352373 1106 5 0.05300228058898426 1106 6 -0.006697926678199906 1106 7 0.024916840281683506 1106 8 -0.03322519413629976 1106 9 -0.10942457834153174 1106 10-11 0.08708476645622198 1106 12-13 0.09456972027042809 1106 14-15 0.019481339826967314 1106 16-17 0.0321102230088286 1106 18-19 0.09638819944811416 1106 20-21 -0.02203230495505437 1106 22-23 0.1371696652201777 1106 24-25 0.09815008840497086 1106 26-27 -0.16015877139312806 1106 28-29 0.07875396413081148 1106 30-31 0.0972943200812253 1106 32-33 0.0044001681903367285 1106 34-35 -0.10081351719026088 1106 36-37 -0.07450399390154416 1106 38-39 0.0733059993405405 1106 40-41 0.04471443352965565 1106 42-43 -0.03402441497546249 1106 44-45 0.038374004319109645 1106 46-47 -0.35572119921854295 1106 48-49 -0.15321258592753395 1106 50-51 0.06608113431566665 1106 52-53 -0.0259226722735022 1106 54-55 -0.06011157404932277 1106 56-57 0.1047529140145258 1106 58-59 0.25001575786493646 1106 60-61 0.25944157169364956 1106 62-63 0.07192602194642106 1106 64-65 0.2927250869529736 1106 66-67 0.14923531479378482 1106 68-69 0.17301890827099342 1106 70-71 0.17641735162127503 1106 72-73 0.469411078959201 1106 74-75 0.44562485994317313 1106 76-77 0.5554765321763959 1106 78-79 0.6685076588085295 1106 80-81 0.8016688885332997 1106 82-83 0.9161034568596911 1106 84-85 1.0090987592386789 1106 86-87 0.9609753542478678 1106 88-89 1.1086059339911642 1106 90-91 1.0774836726074604 1106 92-93 0.8621938513859142 1106 94-95 0.9526114692939096 1106 96-97 1.2445572625911296 1106 98-99 1.525354330464566 1106 100-101 1.320113281276452 1107 1 -0.02719724287372216 1107 2 -0.0345210473849491 1107 3 0.057557497935562196 1107 4 0.004657398962386594 1107 5 -0.03463279163776889 1107 6 -0.07893984329607662 1107 7 -0.03886766032869815 1107 8 -0.040034825753323844 1107 9 0.011609355481304817 1107 10-11 0.04679685713903581 1107 12-13 0.06569857429604298 1107 14-15 0.1928895956697403 1107 16-17 0.02220695501619474 1107 18-19 0.01937543021654875 1107 20-21 -0.013691718417241816 1107 22-23 0.002092050943794277 1107 24-25 0.005565477330378599 1107 26-27 0.2028933451258652 1107 28-29 0.06565868755325255 1107 30-31 0.041361937107247115 1107 32-33 0.09859405283106781 1107 34-35 0.1315543118647824 1107 36-37 0.053614933909216234 1107 38-39 0.08350717749241454 1107 40-41 0.17572217201043117 1107 42-43 0.292197385822611 1107 44-45 0.2417940648269976 1107 46-47 0.40397464907514546 1107 48-49 0.45512235368629206 1107 50-51 0.5566069726843494 1107 52-53 0.3345351515895558 1107 54-55 0.4564109663561169 1107 56-57 0.5375257851541164 1107 58-59 0.5603166251765899 1107 60-61 0.5156099881086007 1107 62-63 0.9124903112780451 1107 64-65 0.9518718666698653 1107 66-67 0.9874801749955004 1107 68-69 1.1930248824372107 1107 70-71 1.1419916677348816 1107 72-73 1.3353414306368556 1107 74-75 1.4004564519088163 1107 76-77 1.4162665867260777 1107 78-79 1.4016061958361874 1107 80-81 1.4282154572986627 1107 82-83 1.7926502251630119 1107 84-85 1.8811061389502193 1107 86-87 1.9737208407992135 1107 88-89 2.082378167008393 1107 90-91 2.2844833441679917 1107 92-93 2.3007781655480386 1107 94-95 2.0700153089627733 1107 96-97 1.9808142858281244 1107 98-99 1.7084909592868698 1107 100-101 1.3963933471559002 1108 1 -7.017139613907375E-4 1108 2 0.10164368291955839 1108 3 0.016385235063552273 1108 4 -0.0405267198712167 1108 5 0.005757804386611554 1108 6 0.049927773046185564 1108 7 0.0539203003283788 1108 8 0.029269854635025183 1108 9 0.06547567780202712 1108 10-11 0.12716084885885692 1108 12-13 0.07908089753367875 1108 14-15 0.19119783276873648 1108 16-17 0.13034220575864452 1108 18-19 0.08965374019717132 1108 20-21 0.07736205878298819 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1.1218257719128033 2316 98-99 1.0348777940994438 2316 100-101 0.35078019271685257 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 15.0 4 54.0 5 98.0 6 208.0 7 481.0 8 1149.0 9 2276.0 10 3573.0 11 5621.0 12 8171.0 13 11460.0 14 15652.0 15 20580.0 16 25978.0 17 31768.0 18 33301.0 19 33194.0 20 35174.0 21 38666.0 22 43368.0 23 47803.0 24 53975.0 25 61890.0 26 71754.0 27 84203.0 28 98867.0 29 115617.0 30 134655.0 31 158337.0 32 184825.0 33 221853.0 34 267461.0 35 325684.0 36 377168.0 37 334904.0 38 108263.0 39 2054.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.71066523980038 18.671527563535705 14.494598396498748 24.12320880016517 2 16.205200005676122 19.97458582874457 38.80904774228773 25.01116642329157 3 19.75584163219085 22.955859469751484 29.37913802690904 27.909160871148625 4 13.691275711934445 16.29240053727842 35.64685565101767 34.36946809976947 5 14.222120874294788 36.90689503732982 34.15790007094355 14.713084017431843 6 34.06667468440298 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23.05664138385391 28-29 22.787417378171707 26.80915854658215 27.633827050012016 22.769597025234127 30-31 22.893063892349726 26.261746208916986 27.647001591835963 23.198188306897325 32-33 22.640353812430966 26.256853865892012 27.729675978797335 23.37311634287969 34-35 23.350443660085052 26.397074002360288 27.555120918193765 22.6973614193609 36-37 23.376993233621963 25.974358099468358 27.57228950463113 23.07635916227855 38-39 22.900360113232022 26.348186265558045 27.56512471351174 23.1863289076982 40-41 23.29406660949983 26.08990329614786 27.579991337129588 23.03603875722272 42-43 22.974846628046787 26.131932143700542 27.488132444988295 23.405088783264375 44-45 23.09002431857074 26.293363008171898 27.604238191253728 23.012374482003633 46-47 23.396500054221086 26.5244824251048 26.99514172310896 23.083875797565153 48-49 23.132498047365903 26.445844470329423 27.108422541910688 23.313234940393986 50-51 23.21342425806661 26.68559956001546 27.102544370062674 22.99843181185526 52-53 23.487012618475568 26.502480993911025 26.97815684854316 23.03234953907025 54-55 23.00881996037977 26.567208449432854 27.172981084448384 23.25099050573899 56-57 23.1795104461719 26.64268327104103 26.954648714141243 23.223157568645824 58-59 23.17612827116885 26.33405785742663 26.938969036223053 23.55084483518147 60-61 22.83953536767627 26.558340517765593 27.286085218627456 23.31603889593068 62-63 22.884567569247565 26.63794920719002 27.318905011445544 23.158578212116872 64-65 22.593917371766242 26.59629634384221 27.19353927668709 23.616247007704462 66-67 22.65003925553665 26.591149869666214 27.060780543307438 23.698030331489697 68-69 23.057960475697005 26.27402491995564 27.178832310978347 23.48918229336901 70-71 22.846742638134998 26.411146235645553 26.92251366295593 23.81959746326352 72-73 22.83301093409745 26.42096646800207 26.921710024465405 23.82431257343507 74-75 22.804840640734245 26.748570320502253 26.686123190266287 23.760465848497216 76-77 22.78999318942848 26.357435414431386 27.038053392718638 23.8145180034215 78-79 22.84181569296214 26.428513998156824 27.061430949723388 23.66823935915765 80-81 22.951311458629284 26.517017493659843 26.898102463395674 23.6335685843152 82-83 22.794225951260643 26.560181644230273 26.959310449205287 23.68628195530379 84-85 22.76518349521156 26.54238716244682 26.87376201079684 23.818667331544784 86-87 22.869281902494198 26.600580813165458 26.680141946521406 23.84999533781894 88-89 22.678814663268586 26.72547991557053 26.45456038031096 24.141145040849914 90-91 22.711220410494295 26.508383007065216 26.669663221979377 24.11073336046111 92-93 22.769014351913945 26.229023885398195 26.865405917989737 24.136555844698123 94-95 22.445292462697257 26.114420304151476 26.987852453832705 24.452434779318562 96-97 22.539701736780486 25.95693022220338 26.920410046473037 24.582957994543094 98-99 22.383943266858193 26.13879444776265 26.587934072586638 24.889328212792524 100-101 22.694302426065047 25.728454627577023 26.77156057460182 24.805682371756106 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 197.0 1 150.0 2 128.5 3 167.0 4 151.0 5 152.5 6 297.0 7 611.5 8 954.0 9 1049.0 10 930.5 11 849.0 12 911.5 13 1048.0 14 1192.0 15 1382.5 16 1599.5 17 1820.5 18 2073.0 19 2362.5 20 2750.0 21 3261.0 22 3992.0 23 4948.5 24 6182.5 25 7853.5 26 10050.0 27 13149.5 28 16625.0 29 20355.0 30 24905.5 31 31147.0 32 40261.5 33 45576.5 34 47442.5 35 51817.0 36 57739.0 37 64359.0 38 69948.0 39 75172.0 40 81192.5 41 88386.5 42 94540.0 43 99579.0 44 104869.5 45 111655.0 46 116525.5 47 120378.5 48 124022.0 49 123412.5 50 121041.0 51 117538.0 52 114602.0 53 113519.5 54 113395.0 55 107011.0 56 96464.0 57 88405.0 58 80716.5 59 69917.5 60 56561.0 61 46711.0 62 39276.5 63 30116.5 64 21715.0 65 16217.5 66 11900.5 67 8961.5 68 6803.5 69 4939.5 70 3733.5 71 2767.5 72 2114.5 73 1671.5 74 1247.5 75 937.5 76 670.0 77 445.0 78 282.5 79 152.0 80 89.5 81 65.0 82 37.5 83 19.0 84 13.0 85 10.5 86 5.0 87 3.5 88 3.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023816730763513715 2 0.011418517727755512 3 0.0019593906160053835 4 0.0 5 1.3513038731071612E-4 6 0.0 7 0.0 8 6.756519365535806E-5 9 3.0404337144911126E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015539994540732353 26-27 0.008580779594230472 28-29 1.8580428255223465E-4 30-31 0.0 32-33 1.5202168572455563E-4 34-35 0.009898300870509955 36-37 9.121301143473337E-4 38-39 0.01150297421982471 40-41 0.011688778502376944 42-43 0.0048309113463581 44-45 6.9254323496742E-4 46-47 3.378259682767903E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.6891298413839515E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.756519365535806E-5 66-67 4.053911619321483E-4 68-69 8.952388159334942E-4 70-71 5.067389524151854E-5 72-73 2.0269558096607415E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0134779048303708E-4 82-83 0.001081043098485729 84-85 0.005506563282911682 86-87 0.003918781232010767 88-89 0.0 90-91 0.0013006299778656426 92-93 3.716085651044693E-4 94-95 0.0 96-97 1.6891298413839515E-5 98-99 0.0 100-101 6.756519365535806E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2960104.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.66232907661934 #Duplication Level Percentage of deduplicated Percentage of total 1 74.90995011466322 40.947523442808595 2 13.7314270836513 15.011835718763017 3 4.8308399185440205 7.921948840317667 4 2.200687859271998 4.811788958337876 5 1.2159193672275719 3.3232492291014153 6 0.7077179091359117 2.3211305545562513 7 0.4856459564162878 1.8582577359049663 8 0.3385697759113792 1.4805610005012064 9 0.25329894582304224 1.2461319298205917 >10 1.218821997914175 11.631061104012497 >50 0.06864037515266964 2.53788183793521 >100 0.03358952272670845 3.501681252740668 >500 0.0024146299864031165 0.8946166993292138 >1k 0.002414629985968767 2.339290340683429 >5k 6.191358938381454E-5 0.17304135518748823 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5113 0.17273041757992288 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4183 0.14131260253018138 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3242 0.10952317891533542 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3230 0.10911778775340326 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.378259682767903E-5 0.0 0.0 0.0 3 0.0 3.378259682767903E-5 0.0 0.0 0.0 4 0.0 3.378259682767903E-5 0.0 0.0 0.0 5 0.0 3.378259682767903E-5 0.0 0.0 0.0 6 0.0 3.378259682767903E-5 0.0 0.0 0.0 7 0.0 3.378259682767903E-5 0.0 3.378259682767903E-5 0.0 8 0.0 3.378259682767903E-5 0.0 3.378259682767903E-5 0.0 9 0.0 3.378259682767903E-5 0.0 3.378259682767903E-5 0.0 10-11 0.0 3.378259682767903E-5 0.0 3.378259682767903E-5 0.0 12-13 0.0 3.378259682767903E-5 0.0 3.378259682767903E-5 0.0 14-15 0.0 3.378259682767903E-5 0.0 3.378259682767903E-5 0.0 16-17 0.0 3.378259682767903E-5 0.0 5.0673895241518545E-5 0.0 18-19 0.0 3.378259682767903E-5 0.0 1.182390888968766E-4 0.0 20-21 0.0 3.378259682767903E-5 0.0 2.364781777937532E-4 0.0 22-23 0.0 3.378259682767903E-5 0.0 3.0404337144911126E-4 0.0 24-25 0.0 3.378259682767903E-5 0.0 6.418693397259015E-4 0.0 26-27 0.0 3.378259682767903E-5 0.0 9.459127111750128E-4 0.0 28-29 0.0 3.378259682767903E-5 0.0 0.00314178150497415 0.0 30-31 1.6891298413839515E-5 3.378259682767903E-5 0.0 0.011621213308721585 0.0 32-33 3.378259682767903E-5 3.378259682767903E-5 0.0 0.028715207303527175 0.0 34-35 3.378259682767903E-5 3.378259682767903E-5 0.0 0.05712637123560524 0.0 36-37 3.378259682767903E-5 3.378259682767903E-5 0.0 0.104303767705459 0.0 38-39 3.378259682767903E-5 3.378259682767903E-5 0.0 0.18055108874553055 0.0 40-41 3.378259682767903E-5 3.378259682767903E-5 0.0 0.2599570825889901 0.0 42-43 3.378259682767903E-5 3.378259682767903E-5 0.0 0.3427920100104591 0.0 44-45 3.378259682767903E-5 3.378259682767903E-5 0.0 0.4467072778523998 0.0 46-47 3.378259682767903E-5 3.378259682767903E-5 0.0 0.571939364292606 0.0 48-49 3.378259682767903E-5 3.378259682767903E-5 0.0 0.7015462970219966 0.0 50-51 3.378259682767903E-5 3.378259682767903E-5 0.0 0.8429940299394886 0.0 52-53 3.378259682767903E-5 3.378259682767903E-5 0.0 0.9851005234951205 0.0 54-55 3.378259682767903E-5 3.378259682767903E-5 0.0 1.140584925394513 0.0 56-57 3.378259682767903E-5 3.378259682767903E-5 0.0 1.3246831868069502 0.0 58-59 3.378259682767903E-5 3.378259682767903E-5 0.0 1.5228181172012873 0.0 60-61 3.378259682767903E-5 3.378259682767903E-5 0.0 1.735158629561664 0.0 62-63 3.378259682767903E-5 3.378259682767903E-5 0.0 1.9417898830581628 1.6891298413839515E-5 64-65 3.378259682767903E-5 3.378259682767903E-5 0.0 2.1636739790223585 3.378259682767903E-5 66-67 3.378259682767903E-5 3.378259682767903E-5 0.0 2.4193913457094753 3.378259682767903E-5 68-69 6.756519365535806E-5 3.378259682767903E-5 0.0 2.707827157424199 3.378259682767903E-5 70-71 6.756519365535806E-5 3.378259682767903E-5 0.0 3.005519400669706 3.378259682767903E-5 72-73 6.756519365535806E-5 3.378259682767903E-5 0.0 3.317062508614562 3.378259682767903E-5 74-75 6.756519365535806E-5 3.378259682767903E-5 0.0 3.6306156810706653 3.378259682767903E-5 76-77 6.756519365535806E-5 3.378259682767903E-5 0.0 3.972529343563604 3.378259682767903E-5 78-79 6.756519365535806E-5 3.378259682767903E-5 0.0 4.352161275414648 3.378259682767903E-5 80-81 6.756519365535806E-5 3.378259682767903E-5 0.0 4.755559264133963 3.378259682767903E-5 82-83 6.756519365535806E-5 3.378259682767903E-5 0.0 5.169294727482548 3.378259682767903E-5 84-85 6.756519365535806E-5 3.378259682767903E-5 0.0 5.593789947920749 3.378259682767903E-5 86-87 6.756519365535806E-5 3.378259682767903E-5 0.0 6.0342305540616135 5.0673895241518545E-5 88-89 6.756519365535806E-5 3.378259682767903E-5 0.0 6.5042478237251125 6.756519365535806E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5725 0.0 33.449013 1 GTATCAA 8445 0.0 32.409817 1 ATCAACG 10190 0.0 26.662415 3 TCAACGC 10230 0.0 26.604143 4 TATCAAC 10605 0.0 25.891714 2 CAACGCA 10590 0.0 25.654902 5 GTACATG 9000 0.0 25.07869 1 AACGCAG 10920 0.0 24.923113 6 TACATGG 9040 0.0 24.43589 2 ACATGGG 9360 0.0 22.886444 3 ACGCAGA 12320 0.0 21.898174 7 CGCAGAG 12655 0.0 21.243427 8 TAAGGTG 1525 0.0 20.244814 5 CATGGGG 6125 0.0 19.851995 4 AGAGTAC 10705 0.0 19.766237 10-11 GAGTACT 6775 0.0 19.664663 12-13 GTAAGGT 1550 0.0 19.305416 4 CTATACG 175 9.947601E-5 18.99898 4 AGTACTT 7130 0.0 18.952028 12-13 GTACTTT 7080 0.0 18.85107 14-15 >>END_MODULE