FastQCFastQC Report
Thu 26 May 2016
SRR938583_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938583_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791373
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69820.2501278044890453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55860.20011657345686157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37330.13373347094780955No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28490.10206446791596822No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46900.045.4307981
GTATCAA79250.041.228991
ATCAACG96900.033.2751463
TATCAAC99250.032.3914382
TCAACGC99850.032.19684
CAACGCA100050.032.1324355
AACGCAG104300.030.752686
ACGCAGA119350.026.7582117
GTACATG90700.026.690441
CGCAGAG120700.026.3403368
TACATGG92750.025.3295252
ACATGGG95000.023.6783583
GCAGAGT129800.023.614559
CTATGCG1452.3275456E-522.9416529
GAGTACT75350.021.63205112-13
CATGGGG57300.020.91514
GTGGTAT25200.020.5717471
GTACTTT80550.020.2587114-15
AGTACTT78850.020.24997312-13
CAGAGTA125750.020.23710-11
Produced by FastQC (version 0.11.5)