##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938583_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2791373 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.109626696253063 31.0 31.0 34.0 28.0 34.0 2 31.15082613466563 31.0 31.0 34.0 27.0 34.0 3 31.1937652187651 31.0 31.0 34.0 27.0 34.0 4 34.84877728630319 37.0 35.0 37.0 32.0 37.0 5 34.82021392339899 37.0 35.0 37.0 32.0 37.0 6 34.83718263377915 37.0 35.0 37.0 32.0 37.0 7 34.74420867436921 37.0 35.0 37.0 31.0 37.0 8 34.714921653250926 37.0 35.0 37.0 31.0 37.0 9 36.20714143183301 39.0 35.0 39.0 32.0 39.0 10-11 36.0359421689613 39.0 35.0 39.0 30.0 39.0 12-13 35.88670915710656 38.0 35.0 39.0 30.0 39.0 14-15 36.84657156173682 39.0 36.0 41.0 30.5 41.0 16-17 36.66318134480774 39.0 36.0 40.5 30.0 41.0 18-19 36.81783283710203 39.0 36.0 41.0 30.0 41.0 20-21 36.796091027605414 39.0 36.0 41.0 30.0 41.0 22-23 36.69665035808543 39.0 36.0 41.0 30.0 41.0 24-25 36.5557591192578 39.0 36.0 41.0 30.0 41.0 26-27 36.34264643241874 39.0 35.5 41.0 29.0 41.0 28-29 36.12800600278071 39.0 35.0 40.0 28.5 41.0 30-31 35.87296753246521 38.5 35.0 40.0 27.0 41.0 32-33 35.60654487952703 38.0 34.5 40.0 27.0 41.0 34-35 35.34871781736085 38.0 34.0 40.0 26.5 41.0 36-37 35.06241534184073 38.0 34.0 40.0 25.0 41.0 38-39 34.91992435263936 38.0 34.0 40.0 25.0 41.0 40-41 34.81949348940468 38.0 34.0 40.0 24.5 41.0 42-43 34.525089266106676 38.0 33.0 40.0 24.0 41.0 44-45 34.193384939956076 38.0 33.0 40.0 23.5 41.0 46-47 33.80170582720403 38.0 33.0 40.0 21.5 41.0 48-49 33.44079078646959 38.0 32.5 40.0 19.5 41.0 50-51 32.74807254351174 36.5 31.5 39.5 18.0 40.5 52-53 32.77488569245314 37.0 31.5 39.0 18.0 40.0 54-55 32.871363124885136 37.0 31.5 40.0 16.0 41.0 56-57 32.5441693030634 37.0 31.0 40.0 13.5 41.0 58-59 32.15843332295613 36.0 31.0 39.0 8.5 41.0 60-61 31.668744198643466 36.0 30.5 39.0 7.5 40.5 62-63 31.08226166836177 35.0 29.5 39.0 4.5 40.0 64-65 30.555466610875726 35.0 29.0 38.0 2.0 40.0 66-67 29.994858623336974 34.5 28.0 38.0 2.0 40.0 68-69 29.384925088836212 34.0 27.5 37.5 2.0 40.0 70-71 28.727103113772326 34.0 26.0 37.0 2.0 39.0 72-73 28.069675747383098 33.5 26.0 36.0 2.0 39.0 74-75 27.37527768592732 33.0 25.0 36.0 2.0 38.5 76-77 26.59411425846707 32.0 23.0 35.0 2.0 37.5 78-79 25.79100983637801 31.5 20.5 35.0 2.0 37.0 80-81 25.079461254371953 31.0 18.5 35.0 2.0 36.0 82-83 24.320694153020753 31.0 12.5 34.0 2.0 36.0 84-85 23.58926144947307 30.0 7.0 34.0 2.0 35.0 86-87 22.797861661626733 29.5 2.0 34.0 2.0 35.0 88-89 21.956339944536253 29.0 2.0 34.0 2.0 35.0 90-91 21.016891866475746 28.5 2.0 34.0 2.0 35.0 92-93 20.041803800495313 26.5 2.0 33.0 2.0 35.0 94-95 19.010904132124228 25.5 2.0 33.0 2.0 35.0 96-97 17.74634883980034 23.0 2.0 32.0 2.0 34.0 98-99 16.208274028587365 10.0 2.0 32.0 2.0 34.0 100-101 12.739994798258778 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2754270074639251 1101 2 0.16252845909924574 1101 3 0.1916232654175012 1101 4 0.1388888430954509 1101 5 0.16335843663976846 1101 6 0.28967331237710425 1101 7 0.27401428126214 1101 8 0.2945597535694162 1101 9 0.43808577112701386 1101 10-11 0.42727234052114227 1101 12-13 0.5064414477308219 1101 14-15 0.6051078339568292 1101 16-17 0.48844690007731373 1101 18-19 0.6665449158992942 1101 20-21 0.6773187812486441 1101 22-23 0.6532301210331255 1101 24-25 0.52887669995512 1101 26-27 0.5818458221679705 1101 28-29 0.6288479701507654 1101 30-31 0.6683295539843854 1101 32-33 0.5971371196680906 1101 34-35 0.5438791162367309 1101 36-37 0.5376766931095602 1101 38-39 0.6026281038573273 1101 40-41 0.5245071747006591 1101 42-43 0.7887384979772705 1101 44-45 0.8334899866965344 1101 46-47 0.7564538590653598 1101 48-49 0.7865654967945304 1101 50-51 1.0724276528451568 1101 52-53 0.7446345851305196 1101 54-55 0.7223382509235776 1101 56-57 0.7531212440896766 1101 58-59 0.7015363999422917 1101 60-61 0.7771913558756545 1101 62-63 0.7680173470013294 1101 64-65 0.7441372518806375 1101 66-67 0.7197325929283771 1101 68-69 0.7879858985406223 1101 70-71 0.7516596442804122 1101 72-73 0.942177694294422 1101 74-75 0.7812242519716079 1101 76-77 0.8720913374320496 1101 78-79 1.0648225203325623 1101 80-81 1.0977731430032414 1101 82-83 1.198916652234118 1101 84-85 1.1400033355313042 1101 86-87 1.3101037285348802 1101 88-89 1.3494204225181115 1101 90-91 1.0374506270859634 1101 92-93 1.0282845689004354 1101 94-95 1.0405913598017555 1101 96-97 1.0309073248396103 1101 98-99 0.8892580041919054 1101 100-101 0.8276895598744254 1102 1 0.4804068823019598 1102 2 0.2925747745404834 1102 3 0.32250849685686234 1102 4 0.30860736651506926 1102 5 0.37117871618654164 1102 6 0.41991963884582617 1102 7 0.38925385339568663 1102 8 0.3560308202029958 1102 9 0.6965417852421965 1102 10-11 0.6552565850005081 1102 12-13 0.700073005638906 1102 14-15 0.8215170777469822 1102 16-17 0.8177179832521162 1102 18-19 0.7671262711773252 1102 20-21 0.8043172374006033 1102 22-23 0.8345459068793488 1102 24-25 0.8159132698555922 1102 26-27 0.7545810522288932 1102 28-29 0.6677780972425396 1102 30-31 0.6948498996960737 1102 32-33 0.7281739101182865 1102 34-35 0.5773179688268115 1102 36-37 0.7290748404919043 1102 38-39 0.6189489209940362 1102 40-41 0.6480417596536583 1102 42-43 0.7519210304394406 1102 44-45 0.8583018302165044 1102 46-47 0.8233944209157755 1102 48-49 0.921071810030277 1102 50-51 0.9588433752833438 1102 52-53 0.9045034269688017 1102 54-55 0.6922205325104258 1102 56-57 0.8507344825866312 1102 58-59 0.7706896957821741 1102 60-61 0.9185058322079094 1102 62-63 0.9888162194408885 1102 64-65 1.142909823766061 1102 66-67 0.9504388344854604 1102 68-69 0.8931207095570812 1102 70-71 1.0677526059874687 1102 72-73 1.185436729940772 1102 74-75 1.2958092049669467 1102 76-77 1.2612528349646475 1102 78-79 1.1548637355615199 1102 80-81 1.2187771956043498 1102 82-83 1.3231263950180079 1102 84-85 1.2805233504184095 1102 86-87 1.5348549898035948 1102 88-89 1.550778043889384 1102 90-91 1.6556244754028953 1102 92-93 1.7389011432119084 1102 94-95 1.4681113471201463 1102 96-97 1.7131207480469541 1102 98-99 1.6123262643302994 1102 100-101 1.6771259174957969 1103 1 0.35771165599262744 1103 2 0.33706909935082763 1103 3 0.2968890385726759 1103 4 0.2621702856014565 1103 5 0.3116351483904225 1103 6 0.29325779549953523 1103 7 0.20449409557148357 1103 8 0.296177603832497 1103 9 0.4230882556244282 1103 10-11 0.43582357221924184 1103 12-13 0.5095087469800248 1103 14-15 0.555271538512649 1103 16-17 0.6781607404296324 1103 18-19 0.6623682375021716 1103 20-21 0.56525215587682 1103 22-23 0.6953032809734978 1103 24-25 0.7357718950051861 1103 26-27 0.7113562060057959 1103 28-29 0.749343372128827 1103 30-31 0.6989101095588452 1103 32-33 0.7505828793457212 1103 34-35 0.6302132232811388 1103 36-37 0.7175609087230868 1103 38-39 0.747611529196476 1103 40-41 0.7486890943663056 1103 42-43 0.6619075595179353 1103 44-45 0.7221198374224826 1103 46-47 0.4413570998029428 1103 48-49 0.5769186357698288 1103 50-51 0.8262132100872961 1103 52-53 0.8747015644581424 1103 54-55 0.8404882192717551 1103 56-57 0.839380612006309 1103 58-59 0.8786008811588104 1103 60-61 1.0018413140135713 1103 62-63 0.9816820827937498 1103 64-65 1.0833132155593255 1103 66-67 1.0580669586327858 1103 68-69 1.1089285781221392 1103 70-71 1.2105121468159616 1103 72-73 1.1588566359665933 1103 74-75 1.3617528032713722 1103 76-77 1.1649677047564815 1103 78-79 1.2128276390779256 1103 80-81 1.5075689377361066 1103 82-83 1.3587002627193776 1103 84-85 1.4853647448199219 1103 86-87 1.589933897821048 1103 88-89 1.6990524446267372 1103 90-91 1.7044710680332038 1103 92-93 1.7439116492415927 1103 94-95 1.8771086389707747 1103 96-97 1.8503553580025454 1103 98-99 1.8549352509603558 1103 100-101 1.5975628845403786 1104 1 0.2554680377807692 1104 2 0.1745041014190889 1104 3 0.27246275872565207 1104 4 0.16378708139692577 1104 5 0.24949344592043587 1104 6 0.2509452521993083 1104 7 0.31356880925067543 1104 8 0.34663959986854564 1104 9 0.45800175666870757 1104 10-11 0.555082754406321 1104 12-13 0.5166365045545547 1104 14-15 0.5896107484118787 1104 16-17 0.6760042936752768 1104 18-19 0.5710598789394439 1104 20-21 0.6284852145418185 1104 22-23 0.5806012500181126 1104 24-25 0.6365037799642366 1104 26-27 0.6810528950130603 1104 28-29 0.5955661960780176 1104 30-31 0.6230994911363581 1104 32-33 0.6684950277732042 1104 34-35 0.7025645179105098 1104 36-37 0.8614736256049156 1104 38-39 0.8982642365871456 1104 40-41 0.9097414708353426 1104 42-43 0.9267830451784178 1104 44-45 0.9152396366362865 1104 46-47 0.8936263492148271 1104 48-49 0.6281731688125021 1104 50-51 0.7652997750243244 1104 52-53 0.737736932976425 1104 54-55 1.0809129358693568 1104 56-57 1.2022810995066564 1104 58-59 1.1290439710126563 1104 60-61 0.9883623480929167 1104 62-63 1.060467443064372 1104 64-65 1.1691338326875673 1104 66-67 1.2038760036548801 1104 68-69 1.3359894154249226 1104 70-71 1.427670585698234 1104 72-73 1.3959690909533826 1104 74-75 1.265206797805078 1104 76-77 1.2491905591585741 1104 78-79 1.2663894876711268 1104 80-81 1.3300858200685965 1104 82-83 1.385366227277263 1104 84-85 1.5432014207831877 1104 86-87 1.6713991142524272 1104 88-89 1.4422160198999876 1104 90-91 1.4450790096448252 1104 92-93 1.4285291226143322 1104 94-95 1.4144511403221216 1104 96-97 1.412923020563987 1104 98-99 1.7450817040844449 1104 100-101 1.5143071638341752 1105 1 0.244682937203752 1105 2 0.09261906830778344 1105 3 0.1523294928178025 1105 4 0.03621653506946387 1105 5 -0.004813678772862318 1105 6 0.2005350872039955 1105 7 0.15531162125547127 1105 8 0.26280904381346915 1105 9 0.3799288381791186 1105 10-11 0.48681861466660337 1105 12-13 0.3464680575091066 1105 14-15 0.4452177045012249 1105 16-17 0.3741501330682553 1105 18-19 0.40869422200562155 1105 20-21 0.43702445508786525 1105 22-23 0.4924948883270517 1105 24-25 0.573901027507894 1105 26-27 0.573814330693132 1105 28-29 0.3792401568465209 1105 30-31 0.5243964863172081 1105 32-33 0.5721258056565262 1105 34-35 0.4803601511046125 1105 36-37 0.5685018875843042 1105 38-39 0.6446810709123483 1105 40-41 0.7292738211887837 1105 42-43 0.7156430556301743 1105 44-45 0.724749224854051 1105 46-47 0.5864801127517225 1105 48-49 0.6605448240397678 1105 50-51 0.752375987144255 1105 52-53 0.6308498783074796 1105 54-55 0.6284410837966874 1105 56-57 0.5653517067825717 1105 58-59 0.7084759193476842 1105 60-61 0.6478642724951094 1105 62-63 0.8658837089396378 1105 64-65 0.9782706698127797 1105 66-67 1.280154141021832 1105 68-69 1.0938783782029198 1105 70-71 1.0204624956055603 1105 72-73 1.1583267220016111 1105 74-75 1.0831184020287452 1105 76-77 1.2273705296860768 1105 78-79 1.3572064262724979 1105 80-81 1.261283885649135 1105 82-83 1.2572175758070792 1105 84-85 1.3093190887142825 1105 86-87 1.427652003269511 1105 88-89 1.3629559944826717 1105 90-91 1.0095561986296886 1105 92-93 1.0642775636011415 1105 94-95 1.344470366240543 1105 96-97 1.6152183031536111 1105 98-99 1.9143215018562678 1105 100-101 1.735480656928896 1106 1 0.16941372917892394 1106 2 0.09947628413446807 1106 3 0.007357150541700719 1106 4 0.07676072170146142 1106 5 0.211636345718496 1106 6 -0.0547586012715513 1106 7 0.26060445559274825 1106 8 0.17198385780820047 1106 9 0.009724112747015567 1106 10-11 0.054040931591153196 1106 12-13 0.08077944578909069 1106 14-15 0.25112320433991187 1106 16-17 -8.57575378127251E-4 1106 18-19 -0.14724462557165197 1106 20-21 0.08605407527014819 1106 22-23 0.08030278548347525 1106 24-25 -0.02610808330025094 1106 26-27 -0.034035163602496254 1106 28-29 0.18118572631018992 1106 30-31 -0.06316449824614523 1106 32-33 0.01164045885276721 1106 34-35 -0.30945029441269867 1106 36-37 -0.0550252842500214 1106 38-39 -0.06689140005391181 1106 40-41 0.31725975468254575 1106 42-43 -0.3063780220790804 1106 44-45 -0.08974145492248908 1106 46-47 0.17609950995303336 1106 48-49 0.3551750687970312 1106 50-51 0.2522536818141816 1106 52-53 0.1571331059518002 1106 54-55 0.21628829554409634 1106 56-57 -0.6248290228162752 1106 58-59 -0.41123174001906904 1106 60-61 0.3204549635026588 1106 62-63 0.24874585477968836 1106 64-65 0.4545841436439808 1106 66-67 0.032469612930800196 1106 68-69 -0.02512573777427818 1106 70-71 -0.0758228752594583 1106 72-73 0.2851695253052 1106 74-75 0.534615706404729 1106 76-77 0.503083089989147 1106 78-79 0.7725192837914534 1106 80-81 0.6827583512160338 1106 82-83 0.6224218971893869 1106 84-85 0.5835775543451511 1106 86-87 0.552408043814065 1106 88-89 0.5981850059127396 1106 90-91 0.8445481352010198 1106 92-93 0.7878286876531533 1106 94-95 0.8421794583787978 1106 96-97 0.7502521712501888 1106 98-99 0.9051428472682055 1106 100-101 0.4941580656260598 1107 1 0.149897833430078 1107 2 0.1570719044137192 1107 3 0.1405623914594294 1107 4 0.11832493806893041 1107 5 0.07913135909493718 1107 6 0.22353918759812075 1107 7 0.2285337306180395 1107 8 0.24279036736077586 1107 9 0.1835644273533319 1107 10-11 0.2797931791167443 1107 12-13 0.4060369895343072 1107 14-15 0.5262732166292281 1107 16-17 0.5565733208906423 1107 18-19 0.5160823622241182 1107 20-21 0.6388339997381607 1107 22-23 0.42882907278861637 1107 24-25 0.521090958606294 1107 26-27 0.4844253877148219 1107 28-29 0.5507332315804163 1107 30-31 0.7061181369598799 1107 32-33 0.7041445710447078 1107 34-35 0.5384377168934691 1107 36-37 0.5896165769186013 1107 38-39 0.5723796193915476 1107 40-41 0.6578056717958845 1107 42-43 0.778462228717359 1107 44-45 0.6612526792956714 1107 46-47 0.6022053114523231 1107 48-49 0.6390958074790447 1107 50-51 0.6774124975029068 1107 52-53 0.73708185716891 1107 54-55 0.8033044039925343 1107 56-57 0.84709699554206 1107 58-59 0.8037320301519415 1107 60-61 0.7338091886617057 1107 62-63 0.8724171323006509 1107 64-65 0.7465687371041767 1107 66-67 0.8322414335446986 1107 68-69 0.8376644789902308 1107 70-71 1.0185613239975133 1107 72-73 1.1097386313997504 1107 74-75 1.097052428278758 1107 76-77 1.223097589010731 1107 78-79 1.4282826744118715 1107 80-81 1.4439012687925832 1107 82-83 1.3252834746640225 1107 84-85 1.3231865888781087 1107 86-87 1.4994732031496838 1107 88-89 1.4126128322175298 1107 90-91 1.270727022640397 1107 92-93 1.5132075292439566 1107 94-95 1.4305361059497699 1107 96-97 1.5195979664157626 1107 98-99 1.3601666932548007 1107 100-101 0.8323220428246643 1108 1 0.3339850574384542 1108 2 0.13373098121421023 1108 3 0.14506025579617088 1108 4 0.1556215636283227 1108 5 0.2014045664812727 1108 6 0.26354673885867186 1108 7 0.31135542358286017 1108 8 0.38921185741442343 1108 9 0.3368128591927828 1108 10-11 0.35046695539559636 1108 12-13 0.4407933455646642 1108 14-15 0.514925884123592 1108 16-17 0.4451307290015336 1108 18-19 0.5278096899117699 1108 20-21 0.4095834623478467 1108 22-23 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2316 74-75 -2.3524317576498 2316 76-77 -2.5420956035115516 2316 78-79 -2.53637251361798 2316 80-81 -2.926270729613332 2316 82-83 -3.076341860456619 2316 84-85 -2.938157697767789 2316 86-87 -3.014751762432521 2316 88-89 -3.3703707378136265 2316 90-91 -3.2639258104876383 2316 92-93 -3.1811488579743603 2316 94-95 -3.602313415824014 2316 96-97 -3.6284442950145674 2316 98-99 -3.4558258072886385 2316 100-101 -2.9575391991143256 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10426.0 3 3679.0 4 4130.0 5 4469.0 6 5378.0 7 6727.0 8 8382.0 9 10541.0 10 13224.0 11 15992.0 12 19409.0 13 22556.0 14 25503.0 15 28769.0 16 32611.0 17 37335.0 18 37087.0 19 35787.0 20 38095.0 21 41522.0 22 45904.0 23 50890.0 24 57044.0 25 63681.0 26 70662.0 27 79422.0 28 90499.0 29 102691.0 30 117684.0 31 136404.0 32 158474.0 33 187763.0 34 225300.0 35 270271.0 36 311190.0 37 291941.0 38 124454.0 39 5477.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66523416017441 18.29955090115347 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28.123744598076073 23.004249149148826 44-45 22.582022782279985 26.575952905610407 28.201817846017814 22.64020646609179 46-47 22.8724006498062 26.81466334836506 27.534552305171005 22.778383696657738 48-49 22.770668517299704 26.766076090683406 27.532883788103472 22.930371603913414 50-51 22.859323738445127 26.956522050675368 27.488706223516633 22.695447987362865 52-53 23.069370197777538 26.745281100702396 27.539415149741654 22.64593355177842 54-55 22.59919059236951 26.844809674913073 27.690673073805282 22.865326658912128 56-57 22.655094761470536 27.12684367895577 27.47486075554783 22.74320080402586 58-59 22.685528574141557 26.621088501187828 27.59517846073362 23.098204463936995 60-61 22.467911212974872 26.82665416781111 27.894664891456504 22.81076972775751 62-63 22.502847043240124 26.851205109468523 27.93669128879113 22.709256558500222 64-65 22.298410753456743 26.78536528128222 27.784558252518494 23.131665712742546 66-67 22.246645330782343 26.924542447392536 27.65689533324555 23.17191688857957 68-69 22.598958312806403 26.56087370734086 27.885214483873266 22.954953495979467 70-71 22.410101700393888 26.742568223872382 27.582542988256048 23.264787087477675 72-73 22.450229055996882 26.704732772781608 27.54857047843749 23.29646769278402 74-75 22.412219435822053 27.03439265116619 27.369113052751835 23.184274860259922 76-77 22.49626574394067 26.760493228172766 27.491542046639278 23.25169898124729 78-79 22.618008222196348 26.804342179508993 27.48004908239214 23.097600515902517 80-81 22.613806071412643 26.944981394393185 27.26147286042687 23.1797396737673 82-83 22.63756712612241 26.95875359746575 27.28669486719158 23.116984409220258 84-85 22.57483199992548 26.810544066947095 27.326949264819422 23.287674668308004 86-87 22.651411897401502 26.779490189165443 27.25539556488078 23.313702348552273 88-89 22.591961971264134 26.84924727028636 27.08631761835867 23.472473140090834 90-91 22.486691283469344 26.83996623387468 27.22625902187541 23.447083460780565 92-93 22.67997052161793 26.37419304900037 27.3751894584503 23.570646970931396 94-95 22.44734001649794 26.31310134547985 27.454068401393457 23.785490236628757 96-97 22.673105743541168 26.02432161837807 27.41955051544692 23.883022122633843 98-99 22.530856400269688 26.205243729145604 27.09524037327075 24.168659497313957 100-101 23.103741392946286 25.55802891209996 27.448049354636783 23.89018034031697 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 410.0 1 289.0 2 263.0 3 373.5 4 337.0 5 315.5 6 547.0 7 1125.5 8 1807.0 9 2127.5 10 1935.0 11 1674.5 12 1732.5 13 1884.0 14 2060.0 15 2280.0 16 2454.5 17 2609.0 18 2839.5 19 3235.5 20 3706.0 21 4354.5 22 5311.5 23 6666.5 24 8329.5 25 10418.5 26 13239.0 27 16723.5 28 19964.5 29 23399.0 30 27850.0 31 34097.5 32 42232.0 33 46679.0 34 48821.5 35 53082.5 36 59149.0 37 65454.0 38 70715.0 39 75525.0 40 80039.0 41 86025.5 42 90529.0 43 94072.0 44 99236.0 45 105080.0 46 107222.0 47 107799.0 48 109383.5 49 108114.5 50 105847.5 51 103115.0 52 100373.5 53 99645.5 54 98566.5 55 92115.0 56 83746.0 57 76719.0 58 70221.0 59 61911.5 60 51084.0 61 42222.0 62 35407.5 63 27689.5 64 21004.5 65 16498.5 66 12502.5 67 9780.0 68 7655.0 69 5624.0 70 4247.5 71 3108.5 72 2366.0 73 1839.0 74 1360.5 75 1125.5 76 813.5 77 515.5 78 347.5 79 215.5 80 127.5 81 75.5 82 47.0 83 39.0 84 26.0 85 17.0 86 15.0 87 9.5 88 6.0 89 7.0 90 5.0 91 2.0 92 4.0 93 4.0 94 1.5 95 3.0 96 3.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008168023406402512 2 0.10457219440039006 3 0.11442397701776151 4 0.11660928152561481 5 0.15752104788575372 6 0.09626087233773487 7 0.025865407453607953 8 0.02898215322710365 9 0.022927785000428105 10-11 0.0523219218642582 12-13 0.05493712234086953 14-15 0.027925325637240168 16-17 0.03209889900059935 18-19 0.03750842327413785 20-21 0.059021134044070785 22-23 0.03209889900059935 24-25 0.04952759806733102 26-27 0.05825090376671265 28-29 0.05407733040335347 30-31 0.06883709199737907 32-33 0.05103223395798411 34-35 0.06376790203244068 36-37 0.05366534676662703 38-39 0.032815392281862726 40-41 0.03736512461788518 42-43 0.03338858690687343 44-45 0.007612741113423394 46-47 0.013864144992446368 48-49 0.00917111400017124 50-51 0.008382971390781526 52-53 0.010120467597845219 54-55 0.011445980168182468 56-57 0.014974709578404605 58-59 0.0201692858675641 60-61 0.025704196465323695 62-63 0.011445980168182468 64-65 0.00969057162908719 66-67 0.010765311550982258 68-69 0.011607191156466728 70-71 0.031257019395114875 72-73 0.028265659945840273 74-75 0.02991359449274604 76-77 0.02351889195747039 78-79 0.005302050281349 80-81 0.005176663957127908 82-83 0.006161842218865053 84-85 0.005516998265728012 86-87 0.01350589835181468 88-89 0.03804579323508538 90-91 0.03591422572332684 92-93 0.030576350777914665 94-95 0.04755724154385674 96-97 0.043759827153160825 98-99 0.026671462395029256 100-101 0.07490937255608619 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2791373.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.53948011430645 #Duplication Level Percentage of deduplicated Percentage of total 1 78.64936731032337 45.25443706355135 2 12.308370649622134 14.164344964668917 3 3.8216154696063294 6.596813019538178 4 1.717340766512422 3.9525957953691724 5 0.9432251696573836 2.713634294640717 6 0.559060044806208 1.9300814598498046 7 0.37760203035004847 1.5208917161513844 8 0.27976525490596305 1.287803785706843 9 0.1915267088654481 0.991831253050981 >10 1.0228734188517914 10.807810031665516 >50 0.08091846826008577 3.1702994667257767 >100 0.043835406512141926 4.428017645259651 >500 0.002624592673926068 1.0920377392845744 >1k 0.00174972844923319 1.6374992816738505 >5k 1.2498060351665642E-4 0.451902482863287 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6982 0.2501278044890453 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5586 0.20011657345686157 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3733 0.13373347094780955 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2849 0.10206446791596822 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.5824664063168916E-5 0.0 18-19 0.0 0.0 0.0 7.164932812633783E-5 0.0 20-21 0.0 0.0 0.0 8.956166015792228E-5 0.0 22-23 0.0 0.0 0.0 2.1494798437901347E-4 0.0 24-25 0.0 3.5824664063168916E-5 0.0 4.119836367264425E-4 0.0 26-27 0.0 3.5824664063168916E-5 0.0 0.0010747399218950673 0.0 28-29 0.0 3.5824664063168916E-5 0.0 0.0028838854570850973 0.0 30-31 0.0 3.5824664063168916E-5 0.0 0.009439798980645009 0.0 32-33 0.0 3.5824664063168916E-5 0.0 0.02299943432855444 0.0 34-35 0.0 3.5824664063168916E-5 0.0 0.04526446304381392 0.0 36-37 0.0 3.5824664063168916E-5 0.0 0.08601501841566855 0.0 38-39 0.0 3.5824664063168916E-5 0.0 0.14915598882700376 0.0 40-41 0.0 3.5824664063168916E-5 0.0 0.2157361269884032 0.0 42-43 0.0 3.5824664063168916E-5 0.0 0.28582708222799313 0.0 44-45 0.0 3.5824664063168916E-5 0.0 0.3737408078390097 0.0 46-47 0.0 3.5824664063168916E-5 0.0 0.4789578461925368 0.0 48-49 0.0 3.5824664063168916E-5 0.0 0.5963194456634782 0.0 50-51 0.0 3.5824664063168916E-5 0.0 0.7176754951774629 0.0 52-53 0.0 3.5824664063168916E-5 0.0 0.8393181420039529 0.0 54-55 0.0 3.5824664063168916E-5 0.0 0.9728366649673834 0.0 56-57 0.0 3.5824664063168916E-5 0.0 1.1323638940406746 0.0 58-59 0.0 3.5824664063168916E-5 0.0 1.3074569396494127 0.0 60-61 0.0 3.5824664063168916E-5 0.0 1.497667993492808 0.0 62-63 0.0 3.5824664063168916E-5 0.0 1.6841174576095708 0.0 64-65 0.0 3.5824664063168916E-5 0.0 1.8774452572264617 0.0 66-67 0.0 3.5824664063168916E-5 0.0 2.1099652393284596 0.0 68-69 0.0 3.5824664063168916E-5 0.0 2.3631202279308425 0.0 70-71 0.0 3.5824664063168916E-5 0.0 2.6285272516428293 0.0 72-73 0.0 3.5824664063168916E-5 0.0 2.9046637622417357 0.0 74-75 0.0 3.5824664063168916E-5 0.0 3.190580406129887 0.0 76-77 0.0 3.5824664063168916E-5 0.0 3.4988874650575186 0.0 78-79 0.0 3.5824664063168916E-5 0.0 3.835424359266927 0.0 80-81 0.0 3.5824664063168916E-5 0.0 4.200674721722965 0.0 82-83 0.0 3.5824664063168916E-5 0.0 4.574558828218228 0.0 84-85 0.0 3.5824664063168916E-5 0.0 4.947421931787691 0.0 86-87 0.0 3.5824664063168916E-5 0.0 5.335008972287115 0.0 88-89 0.0 5.3736996094753374E-5 0.0 5.753423136212896 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4690 0.0 45.430798 1 GTATCAA 7925 0.0 41.22899 1 ATCAACG 9690 0.0 33.275146 3 TATCAAC 9925 0.0 32.391438 2 TCAACGC 9985 0.0 32.1968 4 CAACGCA 10005 0.0 32.132435 5 AACGCAG 10430 0.0 30.75268 6 ACGCAGA 11935 0.0 26.758211 7 GTACATG 9070 0.0 26.69044 1 CGCAGAG 12070 0.0 26.340336 8 TACATGG 9275 0.0 25.329525 2 ACATGGG 9500 0.0 23.678358 3 GCAGAGT 12980 0.0 23.61455 9 CTATGCG 145 2.3275456E-5 22.941652 9 GAGTACT 7535 0.0 21.632051 12-13 CATGGGG 5730 0.0 20.9151 4 GTGGTAT 2520 0.0 20.571747 1 GTACTTT 8055 0.0 20.25871 14-15 AGTACTT 7885 0.0 20.249973 12-13 CAGAGTA 12575 0.0 20.237 10-11 >>END_MODULE