FastQCFastQC Report
Thu 26 May 2016
SRR938583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938583_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791373
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68990.24715435737180233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58360.20907273947265376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40380.14465999348707606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46850.051.529071
GTATCAA76000.039.768731
ATCAACG96850.031.1453553
TATCAAC99250.030.4526312
TCAACGC99150.030.374964
CAACGCA100850.029.8629385
AACGCAG106450.028.3238776
GGACCGA8150.025.6341976
ACGCAGA122350.024.4490077
CGCAGAG123050.024.2327488
GTACATG87600.023.9239391
GTGGTAT21850.023.0543381
TACATGG89750.022.8742832
ACATGGG89000.022.3103033
GCAGAGT136650.021.3345459
TAAGGTG15450.020.907385
GAGTACT81150.020.71282812-13
TGGTATC23550.020.1793562
GTAAGGT14150.020.1425274
CAGAGTA129750.019.92577210-11
Produced by FastQC (version 0.11.5)