##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938583_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2791373 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.779284602953457 31.0 31.0 34.0 30.0 34.0 2 32.003736512461785 31.0 31.0 34.0 30.0 34.0 3 32.06915163254785 33.0 31.0 34.0 30.0 34.0 4 35.74588419390744 37.0 35.0 37.0 33.0 37.0 5 35.64046008899563 37.0 35.0 37.0 33.0 37.0 6 35.63990731442914 37.0 35.0 37.0 33.0 37.0 7 35.578788288057524 37.0 35.0 37.0 33.0 37.0 8 35.552168771425386 37.0 35.0 37.0 33.0 37.0 9 37.13558775555972 39.0 37.0 39.0 33.0 39.0 10-11 37.02818039724537 39.0 37.0 39.0 33.0 39.0 12-13 36.91603146551894 39.0 37.0 39.0 33.0 39.0 14-15 37.96238320711707 40.0 37.0 41.0 33.0 41.0 16-17 37.87119492808736 40.0 37.0 41.0 33.0 41.0 18-19 38.10638044431897 40.0 37.0 41.0 33.0 41.0 20-21 38.07301765117023 40.0 37.0 41.0 33.0 41.0 22-23 37.964989988797626 40.0 37.0 41.0 33.0 41.0 24-25 37.8428162413264 40.0 37.0 41.0 32.0 41.0 26-27 37.6520011836469 40.0 37.0 41.0 32.0 41.0 28-29 37.536468254153064 39.5 36.5 41.0 32.0 41.0 30-31 37.28744313282388 39.0 36.0 41.0 31.0 41.0 32-33 37.09686810039361 39.0 36.0 41.0 31.0 41.0 34-35 37.01003502577405 39.0 36.0 41.0 31.0 41.0 36-37 36.975507572796616 39.0 36.0 41.0 31.0 41.0 38-39 36.815671355995775 39.0 35.0 41.0 30.0 41.0 40-41 36.650325664108664 39.0 35.0 40.5 30.0 41.0 42-43 36.44444812642381 39.0 35.0 40.0 30.0 41.0 44-45 36.16290621138773 38.5 35.0 40.0 30.0 41.0 46-47 35.80304065418703 38.0 35.0 40.0 28.5 41.0 48-49 35.46139731236205 38.0 34.0 40.0 27.5 41.0 50-51 35.086445451754386 38.0 34.0 40.0 26.5 41.0 52-53 34.5921236610084 38.0 33.0 40.0 25.5 41.0 54-55 34.25139492285696 37.5 33.0 40.0 24.0 41.0 56-57 33.91635532048207 37.0 33.0 40.0 24.0 41.0 58-59 33.43496569609293 37.0 32.0 39.0 23.0 41.0 60-61 33.02852592613026 36.0 31.5 39.0 22.0 40.0 62-63 32.53560147640606 35.5 31.0 39.0 20.5 40.0 64-65 31.998648335424896 35.0 30.5 38.5 18.5 40.0 66-67 31.487379866467148 35.0 30.0 38.0 17.5 40.0 68-69 30.985186501409878 34.0 29.0 37.5 13.5 39.5 70-71 30.385022890169104 34.0 29.0 37.0 9.5 39.0 72-73 29.865266662678188 34.0 28.0 36.0 7.5 39.0 74-75 29.253258163634886 33.5 27.5 36.0 7.0 38.5 76-77 28.056642376350275 31.5 26.0 34.5 4.0 36.5 78-79 28.74251918321199 33.0 28.0 35.0 2.0 37.0 80-81 28.346028101582984 33.0 26.5 35.0 2.0 37.0 82-83 27.883560885628686 33.0 26.0 35.0 2.0 36.0 84-85 27.29932223318059 32.5 26.0 35.0 2.0 36.0 86-87 26.652260016844757 32.0 25.0 34.5 2.0 35.0 88-89 25.873704266681663 31.5 23.5 34.0 2.0 35.0 90-91 25.23667761348985 31.0 20.5 34.0 2.0 35.0 92-93 24.373325241735877 31.0 17.5 34.0 2.0 35.0 94-95 23.520198124722135 30.5 6.0 34.0 2.0 35.0 96-97 22.408484104417433 30.0 2.0 34.0 2.0 35.0 98-99 20.99566109581199 29.0 2.0 34.0 2.0 35.0 100-101 17.08444392777318 22.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19656346280583392 1101 2 0.3286356758666429 1101 3 0.24268977756732824 1101 4 0.20967304529808928 1101 5 0.007628927671902375 1101 6 0.11246747706412918 1101 7 0.17166598519766296 1101 8 0.24394097693133432 1101 9 0.2325364002471062 1101 10-11 0.23240544319175882 1101 12-13 0.3100509991207474 1101 14-15 0.410484954715983 1101 16-17 0.3369079564473836 1101 18-19 0.40115887976403997 1101 20-21 0.2909882869969991 1101 22-23 0.2612167121694142 1101 24-25 0.40312246919025085 1101 26-27 0.2627489176224884 1101 28-29 0.20488817565218653 1101 30-31 0.3123862390263312 1101 32-33 0.2606855229857814 1101 34-35 0.36538930331397523 1101 36-37 0.3595336925697339 1101 38-39 0.3851449026365614 1101 40-41 0.5321076643910843 1101 42-43 0.5288332864082221 1101 44-45 0.5667320906280437 1101 46-47 0.5098206284679137 1101 48-49 0.43341966294514833 1101 50-51 0.412249980933332 1101 52-53 0.4268580164219813 1101 54-55 0.4577625039315265 1101 56-57 0.3776270525145833 1101 58-59 0.5057863124490822 1101 60-61 0.5972734770044923 1101 62-63 0.6077229083130717 1101 64-65 0.5295046844005356 1101 66-67 0.3449438322655176 1101 68-69 0.35761398704116587 1101 70-71 0.4391482264771156 1101 72-73 0.3699673812838 1101 74-75 0.4360649504953642 1101 76-77 0.26474419425262496 1101 78-79 0.5299721394132391 1101 80-81 0.6199723482098385 1101 82-83 0.4079887272972087 1101 84-85 0.8129912383875002 1101 86-87 0.9677847067019449 1101 88-89 0.809383837855858 1101 90-91 0.7249692109716186 1101 92-93 0.983938692557043 1101 94-95 0.682451625177972 1101 96-97 0.783125065371209 1101 98-99 0.9493559716497018 1101 100-101 1.290960302788882 1102 1 0.30606502043823625 1102 2 0.3057109108899425 1102 3 0.31739575722161817 1102 4 0.24130952422180485 1102 5 0.19652218160733526 1102 6 0.22472163304792048 1102 7 0.14854975549512517 1102 8 0.23984729843768804 1102 9 0.26743260403504365 1102 10-11 0.38563036255327177 1102 12-13 0.3331300110580031 1102 14-15 0.4305969526207605 1102 16-17 0.38601238673988547 1102 18-19 0.40332791734019935 1102 20-21 0.39102681427623764 1102 22-23 0.45259618174733873 1102 24-25 0.5517425860815948 1102 26-27 0.4704498935549992 1102 28-29 0.4713784714644973 1102 30-31 0.5406287334436684 1102 32-33 0.5257671263822488 1102 34-35 0.4668288037688626 1102 36-37 0.5753440060260289 1102 38-39 0.5954531208705092 1102 40-41 0.43716659251945345 1102 42-43 0.4782076833425819 1102 44-45 0.5525098040788237 1102 46-47 0.4894474169882983 1102 48-49 0.552928526287026 1102 50-51 0.6870128899841461 1102 52-53 0.5708451418320593 1102 54-55 0.5472448323851964 1102 56-57 0.6183558314961957 1102 58-59 0.7167709291061044 1102 60-61 0.6739247051907427 1102 62-63 0.6320234210911764 1102 64-65 0.7065628957423087 1102 66-67 0.49265176929857546 1102 68-69 0.703086997269164 1102 70-71 0.6984902990931126 1102 72-73 0.9241032399114282 1102 74-75 0.8673956234476989 1102 76-77 0.5892275565331069 1102 78-79 0.7059768123104355 1102 80-81 0.89882218141911 1102 82-83 0.8971212087572482 1102 84-85 0.918208204174725 1102 86-87 0.9203085536404387 1102 88-89 0.9638583092257456 1102 90-91 1.1215158158843614 1102 92-93 1.6075395802696626 1102 94-95 1.5856684117685518 1102 96-97 1.3759991728340637 1102 98-99 1.4900580089777407 1102 100-101 1.6638262774808013 1103 1 0.17320510298253922 1103 2 0.15334179045302676 1103 3 0.10362544610133995 1103 4 0.11478974860825986 1103 5 -0.0027569522145611813 1103 6 0.11141200810673269 1103 7 0.19091682414097022 1103 8 0.025133807965090682 1103 9 0.19625103019312462 1103 10-11 0.17900358764656943 1103 12-13 0.21586397432341897 1103 14-15 0.355211489829081 1103 16-17 0.20998778028630483 1103 18-19 0.3054831305008818 1103 20-21 0.22677811893606048 1103 22-23 0.31811027357733224 1103 24-25 0.35481240391262503 1103 26-27 0.27414867666027476 1103 28-29 0.2535460705681558 1103 30-31 0.1544370725241606 1103 32-33 0.06507906737660818 1103 34-35 0.2611627988316627 1103 36-37 0.3433303449647269 1103 38-39 0.2539690206399783 1103 40-41 0.2617380528575737 1103 42-43 0.4732284644411848 1103 44-45 0.269577214546608 1103 46-47 0.4293064396052628 1103 48-49 0.481082805936488 1103 50-51 0.43765447753332154 1103 52-53 0.40991946178686334 1103 54-55 0.4830523733082259 1103 56-57 0.48125993228533304 1103 58-59 0.44539389799407303 1103 60-61 0.6100522342112313 1103 62-63 0.7234254765346506 1103 64-65 0.5809939534137456 1103 66-67 0.6865804013447558 1103 68-69 0.6419491287255887 1103 70-71 0.688797197622911 1103 72-73 0.5918376973254844 1103 74-75 0.5105524973774926 1103 76-77 0.43824393218919866 1103 78-79 0.8464083490912095 1103 80-81 0.8212832779564216 1103 82-83 0.8445740068201886 1103 84-85 0.9297038739287444 1103 86-87 0.763048301667606 1103 88-89 0.9653784876778566 1103 90-91 1.0458619283311101 1103 92-93 1.2590474318617915 1103 94-95 1.124059060972435 1103 96-97 1.3056255588672698 1103 98-99 1.5540661022730013 1103 100-101 1.9307861837644396 1104 1 0.19346339442197547 1104 2 0.19408880035783938 1104 3 0.20503566965108178 1104 4 0.15443842352596704 1104 5 0.04420778077685128 1104 6 -0.002962527169160012 1104 7 0.07412585038376562 1104 8 0.13370554106563048 1104 9 0.19441142059944383 1104 10-11 0.20981825376846786 1104 12-13 0.23885884047018635 1104 14-15 0.28137231672711494 1104 16-17 0.31399512958881104 1104 18-19 0.21261545601204546 1104 20-21 0.29480573465078663 1104 22-23 0.20853866990167091 1104 24-25 0.28792106243653137 1104 26-27 0.2832412814246297 1104 28-29 0.35658539796486366 1104 30-31 0.37371744486175373 1104 32-33 0.3253079463795743 1104 34-35 0.4068175491051065 1104 36-37 0.37232798740186723 1104 38-39 0.41784476261246795 1104 40-41 0.40888385378182335 1104 42-43 0.5193705881764359 1104 44-45 0.47107511710236594 1104 46-47 0.4546631827679519 1104 48-49 0.5336463066000974 1104 50-51 0.7275679658888876 1104 52-53 0.6808522852031231 1104 54-55 0.583337742460948 1104 56-57 0.7397892850848393 1104 58-59 0.7690889273176893 1104 60-61 0.7417949527933416 1104 62-63 0.6978404634272124 1104 64-65 0.6522654385768334 1104 66-67 0.839089848091497 1104 68-69 0.994542574751609 1104 70-71 0.8197392584221497 1104 72-73 0.8075999971293193 1104 74-75 0.8699185439044648 1104 76-77 0.861917010039523 1104 78-79 1.0346789029027725 1104 80-81 0.8500452909953395 1104 82-83 0.8870622887938389 1104 84-85 1.045658534619875 1104 86-87 1.2114113308186525 1104 88-89 1.023681600726679 1104 90-91 1.1719482724845314 1104 92-93 1.4902348923681714 1104 94-95 1.5366673274151026 1104 96-97 1.36799692704043 1104 98-99 1.3443730676150558 1104 100-101 1.6199172860836804 1105 1 0.16280789077653068 1105 2 0.1711219744205863 1105 3 0.15114009788820226 1105 4 0.09651156408517636 1105 5 0.015277782188924505 1105 6 0.08408164175340715 1105 7 0.03669432687757279 1105 8 0.0535194612098806 1105 9 0.16583818167788422 1105 10-11 0.19407188864980895 1105 12-13 0.24382813089741262 1105 14-15 0.26692222264925647 1105 16-17 0.1904386726129914 1105 18-19 0.1844378389397221 1105 20-21 0.22046292192185035 1105 22-23 0.09440629227228925 1105 24-25 0.18603322931701172 1105 26-27 0.09625166438610222 1105 28-29 0.10584677511027962 1105 30-31 0.26490079833020275 1105 32-33 0.25408629797129123 1105 34-35 0.15314271936853885 1105 36-37 0.1874982387629558 1105 38-39 0.1208796335767417 1105 40-41 0.14186529527915326 1105 42-43 0.24805564639125777 1105 44-45 0.16692476873065942 1105 46-47 -0.011125604888839291 1105 48-49 0.18802234528849482 1105 50-51 0.33769663350739876 1105 52-53 0.33210927164601145 1105 54-55 0.3320388765849671 1105 56-57 0.33468120114735456 1105 58-59 0.5921786156840199 1105 60-61 0.4223108180984667 1105 62-63 0.4199139419184448 1105 64-65 0.5013360651631587 1105 66-67 0.35714477170075654 1105 68-69 0.5048830533357709 1105 70-71 0.53783075110411 1105 72-73 0.46919679836132033 1105 74-75 0.515142478181513 1105 76-77 0.5235255182023941 1105 78-79 0.7887400613578883 1105 80-81 0.6344645125689219 1105 82-83 0.701497236275582 1105 84-85 0.7404513641850627 1105 86-87 0.7777892064089791 1105 88-89 0.8827291272452875 1105 90-91 0.9277570222687963 1105 92-93 1.0581304448206517 1105 94-95 1.180776568769776 1105 96-97 1.2008729458149645 1105 98-99 1.3923847600631163 1105 100-101 1.753631315935145 1106 1 -0.05673665847047715 1106 2 0.0038609850231736687 1106 3 0.07491122614911205 1106 4 0.00993259621616005 1106 5 -0.04447542879546518 1106 6 -0.06629733515372749 1106 7 -0.02165603773042335 1106 8 0.03907083411295531 1106 9 -0.3101170992772708 1106 10-11 0.00592255426582966 1106 12-13 0.050582195898911664 1106 14-15 0.04063391863709853 1106 16-17 0.11884837741674659 1106 18-19 0.06167490883112947 1106 20-21 0.10591625671388272 1106 22-23 0.15482803303514459 1106 24-25 0.18977181066968996 1106 26-27 0.048281078727114846 1106 28-29 0.06824324719213593 1106 30-31 0.0033841371215430627 1106 32-33 0.04395259565023224 1106 34-35 0.11421115789354985 1106 36-37 0.1472932843490966 1106 38-39 0.12298069255069066 1106 40-41 0.14360616233005175 1106 42-43 0.12186731895996417 1106 44-45 -0.06850263296984593 1106 46-47 -0.7991883381337104 1106 48-49 -0.383149361581431 1106 50-51 0.04613150934174115 1106 52-53 -0.047445723530223916 1106 54-55 0.09420122997455138 1106 56-57 -0.025286586330445004 1106 58-59 -0.31062547783115235 1106 60-61 -0.14284472653704228 1106 62-63 -0.2895654056846908 1106 64-65 -0.2587366783398508 1106 66-67 -0.414153165900629 1106 68-69 -0.5033769482901214 1106 70-71 -0.23417575779291866 1106 72-73 -0.0027888598697280997 1106 74-75 -0.1104504967069424 1106 76-77 -0.1720150804431242 1106 78-79 0.06665799454198407 1106 80-81 -0.42912062920531113 1106 82-83 0.09998102576115286 1106 84-85 0.2907249731421544 1106 86-87 0.5355793411241514 1106 88-89 0.7470675583907784 1106 90-91 0.7080423722952638 1106 92-93 0.6507361153537197 1106 94-95 0.7791013916148337 1106 96-97 0.9256755944432022 1106 98-99 1.0704968188840454 1106 100-101 0.922617604047737 1107 1 0.10955601142922333 1107 2 0.10135290243901096 1107 3 0.11370644844381417 1107 4 0.10252793289596696 1107 5 0.05535878369614977 1107 6 0.1349883156597329 1107 7 0.030011527001150284 1107 8 0.09084486680251302 1107 9 0.18965814816324666 1107 10-11 0.17756710017584965 1107 12-13 0.20928154976484592 1107 14-15 0.25034950384454646 1107 16-17 0.29964349572497895 1107 18-19 0.28987320156247876 1107 20-21 0.3129000702756102 1107 22-23 0.2237808066377056 1107 24-25 0.17444698295634709 1107 26-27 0.245959640027138 1107 28-29 0.21594715519246677 1107 30-31 0.09160637037263086 1107 32-33 0.12389656112672753 1107 34-35 0.18407647179085984 1107 36-37 0.21686666806860444 1107 38-39 0.31114862832486523 1107 40-41 0.305109369367095 1107 42-43 0.2755458400833888 1107 44-45 0.440999898155205 1107 46-47 0.4764855059622235 1107 48-49 0.5420828354634608 1107 50-51 0.5235880713602157 1107 52-53 0.4749819113449405 1107 54-55 0.35171047058977223 1107 56-57 0.3771256632748745 1107 58-59 0.3237668630931836 1107 60-61 0.3735405267645291 1107 62-63 0.5834794124807914 1107 64-65 0.4492849319377967 1107 66-67 0.4568404602018674 1107 68-69 0.6573857096984064 1107 70-71 0.6821739274116965 1107 72-73 0.7278443704224706 1107 74-75 0.6397821139204716 1107 76-77 0.5797476673518922 1107 78-79 0.9037808663644888 1107 80-81 0.9536749877652824 1107 82-83 0.8871046656654826 1107 84-85 0.9009550550078558 1107 86-87 1.0159515417613036 1107 88-89 1.0363369825893791 1107 90-91 1.1585000899814695 1107 92-93 1.453178083817459 1107 94-95 1.299483388249456 1107 96-97 1.3108164015672195 1107 98-99 1.1659823293157778 1107 100-101 1.1992379612885031 1108 1 0.2090189685584498 1108 2 0.19541277050042538 1108 3 0.1581697066969241 1108 4 0.1948498528618714 1108 5 0.1066514158895373 1108 6 0.19566894829785753 1108 7 0.16374157175278725 1108 8 0.14543457392478842 1108 9 0.206348055417088 1108 10-11 0.31850952528865406 1108 12-13 0.2574270319800078 1108 14-15 0.31114265258122487 1108 16-17 0.35346452621724467 1108 18-19 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2316 72-73 -1.2297873229330811 2316 74-75 -1.118237685369433 2316 76-77 -1.1776317797896212 2316 78-79 -1.2658730536413074 2316 80-81 -1.2358543035904184 2316 82-83 -1.298876422661447 2316 84-85 -1.325579706965911 2316 86-87 -1.4096824702668123 2316 88-89 -1.5093671330544183 2316 90-91 -1.24392138310019 2316 92-93 -1.7256291584113619 2316 94-95 -1.6988939938243064 2316 96-97 -2.0209403551895377 2316 98-99 -1.9792349556635713 2316 100-101 -2.247173983498108 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 19.0 4 41.0 5 93.0 6 208.0 7 462.0 8 1238.0 9 2180.0 10 3511.0 11 5488.0 12 7721.0 13 10859.0 14 14276.0 15 18388.0 16 23315.0 17 27942.0 18 27631.0 19 25049.0 20 26047.0 21 27593.0 22 30059.0 23 34370.0 24 39552.0 25 46325.0 26 54662.0 27 65406.0 28 77586.0 29 92513.0 30 108361.0 31 128212.0 32 154303.0 33 188639.0 34 235617.0 35 304107.0 36 389833.0 37 423123.0 38 189773.0 39 6862.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.20542358502868 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22.45482778546615 26.936564909096706 28.60894978922559 21.999657516211556 20-21 22.81047713795326 26.149783636941393 28.798892158088513 22.24084706701684 22-23 22.64509257630564 26.016587535954528 28.731058156684902 22.60726173105493 24-25 22.60958353821888 26.338079512609752 28.6531473554762 22.39918959369517 26-27 22.163209908432194 26.64583900194872 28.52779841218463 22.663152677434454 28-29 22.264393875479403 26.843794775288615 28.585448827179633 22.306362522052346 30-31 22.44184086538005 26.451419100259965 28.446956350712572 22.65978368364741 32-33 22.14478285580915 26.41257781378572 28.48647764646582 22.956161683939314 34-35 22.811224274092474 26.630546552597618 28.186838109419437 22.371391063890467 36-37 22.869573209634186 26.17907841603125 28.290805032841536 22.660543341493028 38-39 22.323785585068205 26.552032305222227 28.344848180108457 22.779333929601112 40-41 22.735092369647685 26.328708193451245 28.45193650789102 22.484262929010058 42-43 22.572023423230096 26.303020619371075 28.16482026588386 22.960135691514964 44-45 22.539085696986092 26.59439638069357 28.257783327078428 22.608734595241913 46-47 22.78810463524581 26.858574615431184 27.62642255262912 22.726898196693885 48-49 22.693473785122947 26.7969920179066 27.650245954231124 22.859288242739325 50-51 22.82752695999582 26.960479451220515 27.57274416251875 22.63924942626492 52-53 23.104920051888445 26.732131463620235 27.531451368197658 22.631497116293666 54-55 22.561287988163347 26.86889403975882 27.733016160754907 22.836801811322918 56-57 22.66205555473955 27.07995312700954 27.496020775439185 22.761970542811728 58-59 22.714866509289955 26.627139742779377 27.576219490345988 23.081774257584684 60-61 22.444689405536273 26.887305996009847 27.836874541668205 22.831130056785675 62-63 22.45850217297061 26.91767791308834 27.834338309848057 22.789481604092995 64-65 22.275672405475017 26.806447295983453 27.653769650212823 23.264110648328707 66-67 22.16697525512288 26.990986685315725 27.580768012656904 23.26127004690449 68-69 22.53535332724815 26.66635613724087 27.752605983842315 23.045684551668664 70-71 22.385795794574744 26.799058025439475 27.515193701445224 23.299952478540558 72-73 22.437876864473253 26.859574227221277 27.376918478962757 23.32563042934271 74-75 22.414610763466428 27.17923708718525 27.13686518514909 23.26928696419924 76-77 22.418299974229324 26.86386668353648 27.45578573136205 23.26204761087214 78-79 22.448534943811428 26.98178518744545 27.459270250023426 23.110409618719693 80-81 22.44893425080425 27.120331608222838 27.251466284197996 23.179267856774917 82-83 22.534199495984794 27.133965407010912 27.263069131505535 23.068765965498756 84-85 22.448972279466602 27.0687028726657 27.21677288353492 23.265551964332772 86-87 22.55151037874836 27.03123298154698 27.121215812615006 23.29604082708965 88-89 22.400469696086912 27.15874203914433 26.913155524083127 23.52763274068563 90-91 22.28400572839708 27.137752612868972 27.153143245581646 23.42509841315231 92-93 22.42962088101689 26.783212999429896 27.218532794736245 23.56863332481697 94-95 22.22356400199953 26.748129023472984 27.24702623577619 23.7812807387513 96-97 22.370682850599362 26.495521423109984 27.28567616951271 23.848119556777945 98-99 22.144302530243365 26.728358034552734 26.960534024490755 24.166805410713145 100-101 22.295201262350485 26.543452058485467 27.260958858954403 23.900387820209644 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 392.0 1 287.5 2 265.0 3 369.0 4 339.0 5 320.0 6 553.0 7 1170.5 8 1837.0 9 2116.5 10 1929.0 11 1638.5 12 1618.0 13 1751.0 14 1983.5 15 2179.5 16 2351.5 17 2528.0 18 2748.0 19 3163.5 20 3656.0 21 4325.5 22 5290.5 23 6502.5 24 8011.5 25 10144.0 26 13141.0 27 16651.0 28 19969.0 29 23650.5 30 28153.5 31 34389.0 32 43058.0 33 47468.0 34 49317.5 35 54033.0 36 60133.0 37 66635.0 38 71684.5 39 75796.5 40 80531.5 41 86933.5 42 91594.5 43 95398.5 44 100651.0 45 105832.0 46 108085.0 47 109333.5 48 110348.0 49 108727.5 50 107033.5 51 104068.0 52 100408.5 53 99727.5 54 99844.5 55 93438.5 56 84023.0 57 76202.0 58 69183.5 59 60798.5 60 49405.5 61 40375.0 62 33919.0 63 25985.5 64 19181.5 65 14804.0 66 10991.5 67 8576.5 68 6597.5 69 4762.5 70 3531.0 71 2493.0 72 1916.5 73 1528.5 74 1183.0 75 975.0 76 669.5 77 400.0 78 236.5 79 140.0 80 79.5 81 48.5 82 27.5 83 12.5 84 6.5 85 4.0 86 2.5 87 2.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.224219765685202E-4 2 0.05069189964938401 3 0.0 4 0.0 5 0.04367026549300291 6 3.582466406316891E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006466351863401988 26-27 0.001916619527379537 28-29 1.253863242210912E-4 30-31 0.001594197550811017 32-33 4.8363296485278036E-4 34-35 0.011284769179898207 36-37 0.022336678043385818 38-39 0.004352696683675023 40-41 0.001218038578147743 42-43 0.0014867235586215099 44-45 0.008257585066560436 46-47 0.0 48-49 0.0 50-51 5.373699609475337E-4 52-53 0.0 54-55 0.0015404605547162632 56-57 0.0 58-59 0.022103817726975217 60-61 0.0 62-63 0.009421886648613424 64-65 0.014491076613551824 66-67 0.0060543682266755465 68-69 0.015780764519825907 70-71 8.956166015792228E-5 72-73 5.3736996094753374E-5 74-75 0.021799308082438285 76-77 0.0494380364071731 78-79 0.029179188879451083 80-81 0.02616991709814489 82-83 0.035143995445968704 84-85 0.01660473179327879 86-87 0.01021002925800314 88-89 0.083668502919531 90-91 0.02559672247313419 92-93 0.023859226266070496 94-95 0.02572210879735528 96-97 0.03702479030928507 98-99 0.0524293958564477 100-101 0.013935794320572707 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2791373.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.903078521924265 #Duplication Level Percentage of deduplicated Percentage of total 1 76.64611449393625 42.08107642460979 2 13.271263053818988 14.572663950578727 3 4.308808067791077 7.097004830455028 4 1.897449830626952 4.167033481692937 5 1.025700998272297 2.815707122408002 6 0.6359451431685443 2.0949207678611375 7 0.41410794442952165 1.5915060692696672 8 0.28456435538437963 1.2498767318567483 9 0.22137972431088945 1.0938985548302438 >10 1.1489953559550818 11.602451337922506 >50 0.09217460192500755 3.511334946560083 >100 0.048736053783686444 4.8185858228457095 >500 0.002347582979502524 0.9001859776849199 >1k 0.0022823723411418773 1.9478072596466265 >5k 1.3042127663667868E-4 0.4559467217778066 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6899 0.24715435737180233 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5836 0.20907273947265376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4038 0.14465999348707606 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 7.164932812633783E-5 0.0 6 0.0 0.0 0.0 7.164932812633783E-5 0.0 7 0.0 0.0 0.0 7.164932812633783E-5 0.0 8 0.0 0.0 0.0 7.164932812633783E-5 0.0 9 0.0 0.0 0.0 7.164932812633783E-5 0.0 10-11 0.0 0.0 0.0 7.164932812633783E-5 0.0 12-13 0.0 0.0 0.0 7.164932812633783E-5 0.0 14-15 0.0 0.0 0.0 1.0747399218950673E-4 0.0 16-17 0.0 0.0 0.0 1.4329865625267566E-4 0.0 18-19 0.0 0.0 0.0 1.7912332031584457E-4 0.0 20-21 0.0 0.0 0.0 1.9703565234742903E-4 0.0 22-23 0.0 0.0 0.0 3.224219765685202E-4 0.0 24-25 0.0 0.0 0.0 5.194576289159492E-4 0.0 26-27 0.0 0.0 0.0 0.0011463892500214053 0.0 28-29 0.0 0.0 0.0 0.0029734471172430197 0.0 30-31 0.0 0.0 0.0 0.00972639629315036 0.0 32-33 0.0 0.0 0.0 0.023536804289501976 0.0 34-35 0.0 0.0 0.0 0.04608843031726681 0.0 36-37 0.0 0.0 0.0 0.08692854734927938 0.0 38-39 0.0 0.0 0.0 0.15053523839343577 0.0 40-41 0.0 0.0 0.0 0.21774230817594065 0.0 42-43 0.0 0.0 0.0 0.2884422827046045 0.0 44-45 0.0 0.0 0.0 0.37714415092501075 0.0 46-47 0.0 0.0 0.0 0.48291647157151696 0.0 48-49 0.0 0.0 0.0 0.6002959833744899 0.0 50-51 0.0 0.0 0.0 0.7219386302009799 0.0 52-53 0.0 0.0 0.0 0.8436887510196596 0.0 54-55 0.0 0.0 0.0 0.9773505726393428 0.0 56-57 0.0 0.0 0.0 1.1371823113571708 0.0 58-59 0.0 0.0 0.0 1.3118096363330878 0.0 60-61 0.0 0.0 0.0 1.5022356381608621 0.0 62-63 0.0 0.0 0.0 1.6891508229104457 0.0 64-65 0.0 0.0 0.0 1.8819591648984209 0.0 66-67 0.0 0.0 0.0 2.1143000236801033 0.0 68-69 0.0 0.0 0.0 2.3682610672239073 0.0 70-71 0.0 0.0 0.0 2.6353339378148313 3.5824664063168916E-5 72-73 0.0 0.0 0.0 2.9141035612223805 3.5824664063168916E-5 74-75 0.0 0.0 0.0 3.2031011262199645 3.5824664063168916E-5 76-77 0.0 0.0 0.0 3.516602761436755 3.5824664063168916E-5 78-79 0.0 0.0 0.0 3.860035903478324 3.5824664063168916E-5 80-81 0.0 0.0 0.0 4.232970656375912 3.5824664063168916E-5 82-83 0.0 0.0 0.0 4.615990052207283 3.5824664063168916E-5 84-85 0.0 0.0 0.0 4.9970390915151786 3.5824664063168916E-5 86-87 0.0 0.0 0.0 5.395481005225744 3.5824664063168916E-5 88-89 0.0 0.0 0.0 5.829031089718214 3.5824664063168916E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4685 0.0 51.52907 1 GTATCAA 7600 0.0 39.76873 1 ATCAACG 9685 0.0 31.145355 3 TATCAAC 9925 0.0 30.452631 2 TCAACGC 9915 0.0 30.37496 4 CAACGCA 10085 0.0 29.862938 5 AACGCAG 10645 0.0 28.323877 6 GGACCGA 815 0.0 25.634197 6 ACGCAGA 12235 0.0 24.449007 7 CGCAGAG 12305 0.0 24.232748 8 GTACATG 8760 0.0 23.923939 1 GTGGTAT 2185 0.0 23.054338 1 TACATGG 8975 0.0 22.874283 2 ACATGGG 8900 0.0 22.310303 3 GCAGAGT 13665 0.0 21.334545 9 TAAGGTG 1545 0.0 20.90738 5 GAGTACT 8115 0.0 20.712828 12-13 TGGTATC 2355 0.0 20.179356 2 GTAAGGT 1415 0.0 20.142527 4 CAGAGTA 12975 0.0 19.925772 10-11 >>END_MODULE