FastQCFastQC Report
Thu 26 May 2016
SRR938582_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938582_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2757312
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71420.2590203792679247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55260.20041257572592439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37680.13665482905090173No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.045.311971
GTATCAA75950.040.3598981
ATCAACG95350.031.6993143
TCAACGC96400.031.2554454
TATCAAC97050.031.2419822
CAACGCA97350.030.949315
AACGCAG101950.029.640726
GTACATG89000.025.8314671
ACGCAGA117500.025.5930277
CGCAGAG118700.025.2142268
TACATGG91100.024.9879472
ACATGGG89000.024.8833793
GCAGAGT130900.022.42719
AGGTAAG13850.021.9605732
CATGGGG54600.021.8471374
GTAAGGT14200.021.7539674
GGTAAGG15150.021.3309273
GAGTACT78800.020.58511712-13
TAAGGTG16800.020.083845
GTACTTT79250.020.02052314-15
Produced by FastQC (version 0.11.5)