##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938582_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2757312 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.050374785298146 31.0 31.0 34.0 28.0 34.0 2 31.146941296451036 31.0 31.0 34.0 27.0 34.0 3 31.21411106178771 31.0 31.0 34.0 27.0 34.0 4 34.84573671749878 37.0 35.0 37.0 32.0 37.0 5 34.85388958521923 37.0 35.0 37.0 32.0 37.0 6 34.88668348014298 37.0 35.0 37.0 32.0 37.0 7 34.80733700067312 37.0 35.0 37.0 32.0 37.0 8 34.76874107826753 37.0 35.0 37.0 32.0 37.0 9 36.275244513497206 39.0 35.0 39.0 32.0 39.0 10-11 36.11955756185735 39.0 35.0 39.0 31.0 39.0 12-13 35.964181964173804 38.0 35.0 39.0 30.0 39.0 14-15 36.951972428219946 39.0 36.0 41.0 31.0 41.0 16-17 36.79558007943969 39.0 36.0 41.0 30.5 41.0 18-19 36.77999569870947 39.0 36.0 41.0 30.0 41.0 20-21 36.87117544187963 39.0 36.0 41.0 30.0 41.0 22-23 36.785171754230205 39.0 36.0 41.0 30.0 41.0 24-25 36.66843813830281 39.0 36.0 41.0 30.0 41.0 26-27 36.47924173978136 39.0 36.0 41.0 30.0 41.0 28-29 36.292284841178656 39.0 35.0 40.5 29.0 41.0 30-31 36.06899853915698 39.0 35.0 40.0 28.5 41.0 32-33 35.79189895811572 38.0 35.0 40.0 27.0 41.0 34-35 35.54006565089479 38.0 34.5 40.0 27.0 41.0 36-37 35.24598775909291 38.0 34.0 40.0 25.5 41.0 38-39 34.987210551435595 38.0 34.0 40.0 25.0 41.0 40-41 34.92142419863983 38.0 34.0 40.0 25.0 41.0 42-43 34.74350381821136 38.0 33.5 40.0 24.0 41.0 44-45 34.457489032797156 38.0 33.0 40.0 24.0 41.0 46-47 34.100477747893606 38.0 33.0 40.0 23.0 41.0 48-49 33.73658548615464 38.0 33.0 40.0 21.0 41.0 50-51 33.00013219396281 37.0 31.5 39.5 19.0 40.5 52-53 33.06995816940557 37.0 32.0 39.5 19.5 40.0 54-55 33.18861612323886 37.0 32.0 40.0 18.5 41.0 56-57 32.88057009870482 37.0 31.5 40.0 16.5 41.0 58-59 32.53828620047351 37.0 31.0 40.0 13.5 41.0 60-61 32.0797359892533 36.0 31.0 39.0 9.5 41.0 62-63 31.546741536685005 35.5 30.0 39.0 7.5 40.0 64-65 31.046766198384514 35.0 29.5 39.0 6.0 40.0 66-67 30.4959061578813 35.0 29.0 38.0 2.0 40.0 68-69 29.923514821681408 34.5 28.0 38.0 2.0 40.0 70-71 29.2821731817074 34.0 27.5 37.0 2.0 39.0 72-73 28.712377308044935 34.0 26.0 36.5 2.0 39.0 74-75 28.078123911983845 33.5 26.0 36.0 2.0 39.0 76-77 27.32941266711928 33.0 25.5 35.0 2.0 38.0 78-79 26.531997285762365 32.0 23.0 35.0 2.0 37.0 80-81 25.85501531926746 32.0 21.0 35.0 2.0 36.5 82-83 25.181162124561894 31.0 19.0 34.5 2.0 36.0 84-85 24.510214114325837 31.0 16.0 34.0 2.0 35.5 86-87 23.819605108163312 30.5 8.5 34.0 2.0 35.0 88-89 23.017687334621545 30.0 2.0 34.0 2.0 35.0 90-91 22.1722213518093 29.0 2.0 34.0 2.0 35.0 92-93 21.303194016491425 29.0 2.0 34.0 2.0 35.0 94-95 20.328763665482906 28.0 2.0 34.0 2.0 35.0 96-97 19.080376830768515 25.5 2.0 33.0 2.0 35.0 98-99 17.598011940614626 22.5 2.0 32.5 2.0 34.0 100-101 14.203333536429682 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6594479161004791 1101 2 0.7389702038712969 1101 3 0.6596049615352548 1101 4 0.6572675856559655 1101 5 0.5609218695523666 1101 6 0.6712979397454433 1101 7 0.5273682540592333 1101 8 0.6214995271631167 1101 9 0.7157512014292351 1101 10-11 0.7643249005499939 1101 12-13 0.7604108748707432 1101 14-15 0.8858005611511857 1101 16-17 0.8835488385061652 1101 18-19 0.8080713073940871 1101 20-21 0.9574096687174318 1101 22-23 0.9895277239570248 1101 24-25 1.076098693818956 1101 26-27 1.063315835893441 1101 28-29 1.0470842258387094 1101 30-31 1.087479817870161 1101 32-33 0.9051401795147882 1101 34-35 1.007506409674832 1101 36-37 1.0981298968511695 1101 38-39 1.0452587442321786 1101 40-41 1.215849855795625 1101 42-43 1.3392884294709972 1101 44-45 1.3339465230341858 1101 46-47 1.5925185795346621 1101 48-49 1.4999893447729775 1101 50-51 1.384015294349922 1101 52-53 1.5054834884646127 1101 54-55 1.5224239954295982 1101 56-57 1.3411839319270982 1101 58-59 1.4023419428939263 1101 60-61 1.294801400394654 1101 62-63 1.2222572119969257 1101 64-65 1.4451254788003922 1101 66-67 1.3670571113463588 1101 68-69 1.4193937173833255 1101 70-71 1.5189770173972796 1101 72-73 1.4852201681592128 1101 74-75 1.2871122353763162 1101 76-77 1.3182721448930828 1101 78-79 1.2639044897969782 1101 80-81 1.5730317122068698 1101 82-83 1.52604584013935 1101 84-85 1.7690965827627068 1101 86-87 1.6264545440711693 1101 88-89 1.6913909623322354 1101 90-91 1.6309651104699334 1101 92-93 1.5834819205578192 1101 94-95 1.4390793885847266 1101 96-97 1.2211347464950641 1101 98-99 1.473446658981647 1101 100-101 1.4583034818007476 1102 1 0.6586646814180277 1102 2 0.6548663271582384 1102 3 0.5294071265221909 1102 4 0.5434844042983187 1102 5 0.5964984706295269 1102 6 0.5512646650693895 1102 7 0.6269401625538791 1102 8 0.5614088750007937 1102 9 0.6210011347732376 1102 10-11 0.7274094203601607 1102 12-13 0.7232727103104111 1102 14-15 0.8950786499903103 1102 16-17 0.9569532004749064 1102 18-19 0.8396432158887279 1102 20-21 0.8181086502137234 1102 22-23 0.7849443506300915 1102 24-25 0.7446743883744986 1102 26-27 0.6420897920604531 1102 28-29 0.5319920272571395 1102 30-31 0.6035973457323678 1102 32-33 0.6906223956935378 1102 34-35 0.5736536927762046 1102 36-37 0.6585027438623143 1102 38-39 0.7082174175111291 1102 40-41 0.7053527619973039 1102 42-43 0.9129481105886867 1102 44-45 0.813401756863442 1102 46-47 0.9450464950681763 1102 48-49 0.687957563191361 1102 50-51 0.7942171820478876 1102 52-53 0.79302062918876 1102 54-55 0.8215908487330736 1102 56-57 0.8665876539982378 1102 58-59 0.9165568418434304 1102 60-61 0.890393572313819 1102 62-63 0.7894739773144721 1102 64-65 0.7910142432647405 1102 66-67 0.7020467130100947 1102 68-69 0.7720621970932413 1102 70-71 0.819843225470656 1102 72-73 0.8757944762699168 1102 74-75 0.7232451207813178 1102 76-77 0.8302691587042084 1102 78-79 0.9856290138731225 1102 80-81 1.068388615102407 1102 82-83 0.9482231984286891 1102 84-85 0.8557073650375422 1102 86-87 0.9570058158676851 1102 88-89 1.1120391786176285 1102 90-91 1.1749892132801527 1102 92-93 1.2640776652386698 1102 94-95 1.4442713045448379 1102 96-97 1.4425958460524662 1102 98-99 1.6483780299622879 1102 100-101 1.247964656012872 1103 1 0.6525643123833582 1103 2 0.5941284965158076 1103 3 0.597421940587072 1103 4 0.6434163754916327 1103 5 0.6401801254456814 1103 6 0.5351916762918805 1103 7 0.5672657707285254 1103 8 0.5019468417008355 1103 9 0.47219830596013423 1103 10-11 0.5437219709547847 1103 12-13 0.5816820461838148 1103 14-15 0.7671838761089234 1103 16-17 0.6995643790112283 1103 18-19 0.7400937781243826 1103 20-21 0.7390872032373039 1103 22-23 0.7859970892113992 1103 24-25 0.806092918631272 1103 26-27 0.7388055193081513 1103 28-29 0.6890290990470831 1103 30-31 0.8898344143945423 1103 32-33 0.9023393184868098 1103 34-35 0.9454514928900366 1103 36-37 0.9611019134417091 1103 38-39 0.9383356592319174 1103 40-41 0.9174118865616308 1103 42-43 0.9229320478885938 1103 44-45 0.909138206763906 1103 46-47 0.9373573467945917 1103 48-49 0.8469643014590673 1103 50-51 0.9278061275520031 1103 52-53 1.0535863918089703 1103 54-55 1.0140932346798763 1103 56-57 1.0016398567446672 1103 58-59 0.9009048661889381 1103 60-61 1.1937545163709231 1103 62-63 1.20931995153596 1103 64-65 1.1766447731774576 1103 66-67 1.2091288286771729 1103 68-69 1.273523396597902 1103 70-71 1.3107949414798128 1103 72-73 1.205179216828295 1103 74-75 1.2147491335755092 1103 76-77 1.2281802669070494 1103 78-79 1.1567747580806937 1103 80-81 1.131118130015448 1103 82-83 1.077240673971449 1103 84-85 1.1287063900814438 1103 86-87 1.2327165275856338 1103 88-89 1.212978823937739 1103 90-91 1.432919013971798 1103 92-93 1.4737002226526386 1103 94-95 1.4484528332216762 1103 96-97 1.3073656489993937 1103 98-99 1.4736205446329294 1103 100-101 1.40877253204488 1104 1 0.5044492786157839 1104 2 0.5223233712882092 1104 3 0.6042385483999162 1104 4 0.5552901792642189 1104 5 0.5032811941587028 1104 6 0.4467456208823677 1104 7 0.4726942056640695 1104 8 0.4151084680230497 1104 9 0.5377050138850379 1104 10-11 0.5253284706850394 1104 12-13 0.5908328406871348 1104 14-15 0.685620916095047 1104 16-17 0.7305073938949889 1104 18-19 0.7383973797281271 1104 20-21 0.8272804194806085 1104 22-23 0.8437557641770965 1104 24-25 0.8464820952780556 1104 26-27 0.742798700969935 1104 28-29 0.8323354633130506 1104 30-31 0.6718851230563416 1104 32-33 0.5899747977517933 1104 34-35 0.616420019305572 1104 36-37 0.7849280548684447 1104 38-39 0.9471529509550791 1104 40-41 0.7701072159653393 1104 42-43 0.7952857389344388 1104 44-45 0.8447560640907383 1104 46-47 0.8381182069988284 1104 48-49 0.871835915201224 1104 50-51 0.7641955602991146 1104 52-53 0.7521965151457621 1104 54-55 0.8531659971198096 1104 56-57 0.9712608881713933 1104 58-59 0.7313901483405445 1104 60-61 0.900677118302248 1104 62-63 1.0093380143287192 1104 64-65 1.1332181988293613 1104 66-67 1.0838025969364686 1104 68-69 1.079706302540533 1104 70-71 0.9759558207983936 1104 72-73 1.1607497381907734 1104 74-75 0.6994990517471997 1104 76-77 0.9537037517987947 1104 78-79 1.0095280561376754 1104 80-81 1.0927529312268938 1104 82-83 1.0007114029961457 1104 84-85 1.0644747928660188 1104 86-87 1.1297703889168957 1104 88-89 1.0861932596365236 1104 90-91 0.9953252974277298 1104 92-93 1.0342644872607032 1104 94-95 0.8953327819377179 1104 96-97 0.8101056162627458 1104 98-99 0.820061515572597 1104 100-101 0.5044174156756362 1105 1 0.5604006993257009 1105 2 0.5361341803383368 1105 3 0.5250748664982972 1105 4 0.5219981131362275 1105 5 0.4835684225769512 1105 6 0.5923551127730491 1105 7 0.4249439567796074 1105 8 0.3987147934174473 1105 9 0.5449163847148029 1105 10-11 0.681225187169396 1105 12-13 0.7394771996055027 1105 14-15 0.7164672278178585 1105 16-17 0.7881954920989571 1105 18-19 0.7083967461229079 1105 20-21 0.7869592237031 1105 22-23 0.8692125431856681 1105 24-25 0.7568298247084826 1105 26-27 0.7599514269831005 1105 28-29 0.7439416704004813 1105 30-31 0.7769255864627098 1105 32-33 0.8643895383924445 1105 34-35 0.9984723673356157 1105 36-37 0.88111315224414 1105 38-39 0.898396774100938 1105 40-41 0.804027660391597 1105 42-43 0.6822441291743218 1105 44-45 0.8029160005823357 1105 46-47 0.7796671690655117 1105 48-49 0.8427445114647796 1105 50-51 0.539320971425397 1105 52-53 0.6349266422836948 1105 54-55 0.7231885149649742 1105 56-57 0.8666067709676852 1105 58-59 0.9758466444577678 1105 60-61 1.0659614547014868 1105 62-63 1.104620766077474 1105 64-65 1.2691465981245642 1105 66-67 1.2962188753127712 1105 68-69 0.9872203377886173 1105 70-71 0.9845110848785481 1105 72-73 0.8570590578861683 1105 74-75 0.9001358186216528 1105 76-77 0.894322831271122 1105 78-79 0.8131829128874379 1105 80-81 0.9879365243957388 1105 82-83 1.3257827537009739 1105 84-85 1.1777981465983807 1105 86-87 1.0981854235401691 1105 88-89 0.9235860646603911 1105 90-91 0.7671010786903771 1105 92-93 0.8656938177771103 1105 94-95 1.0346924021111477 1105 96-97 1.0657940716823724 1105 98-99 1.1816707249543938 1105 100-101 0.738665728494567 1106 1 0.29597281717838797 1106 2 0.24924104573239703 1106 3 0.20375491377976118 1106 4 0.40334235711182487 1106 5 0.4498288145653291 1106 6 0.09660671311152669 1106 7 0.23065260390232112 1106 8 0.20463296965185407 1106 9 0.2374234623106659 1106 10-11 0.03345875237152285 1106 12-13 0.06016377431132014 1106 14-15 0.3653449737586385 1106 16-17 0.16205354583321707 1106 18-19 -0.09831100942948723 1106 20-21 0.0687442982712625 1106 22-23 0.3274099725584776 1106 24-25 0.28707904584515376 1106 26-27 0.0684821614494453 1106 28-29 0.0033869956572303295 1106 30-31 0.2628974737577323 1106 32-33 0.375404062718502 1106 34-35 0.27391792557059347 1106 36-37 0.09525719934809729 1106 38-39 0.14089621182667145 1106 40-41 0.4543631591112245 1106 42-43 -0.07703780876069288 1106 44-45 0.18292585172841314 1106 46-47 -0.02165006557381588 1106 48-49 0.3552747179480278 1106 50-51 0.1754893017179171 1106 52-53 0.42217737576164893 1106 54-55 0.2676594300045778 1106 56-57 -0.2036137212315836 1106 58-59 -0.6205863556461182 1106 60-61 0.13461003731647025 1106 62-63 0.3552085696173357 1106 64-65 0.4998633011499578 1106 66-67 -0.1622163229557252 1106 68-69 -0.21132759521047717 1106 70-71 -0.30376762716541705 1106 72-73 0.06826969742650846 1106 74-75 0.02160363938778076 1106 76-77 0.06273967115860657 1106 78-79 0.1589021020223811 1106 80-81 0.21089724638615692 1106 82-83 0.2641845357321948 1106 84-85 0.2739885871289225 1106 86-87 0.4360283604171933 1106 88-89 0.19086742569695048 1106 90-91 0.2627906664803028 1106 92-93 0.24673454710987386 1106 94-95 0.13739286927786054 1106 96-97 0.16228701829203374 1106 98-99 -0.10519688038252184 1106 100-101 -0.15538522061431692 1107 1 0.37839437315219726 1107 2 0.32210987122148893 1107 3 0.2505596209109662 1107 4 0.29926166175530255 1107 5 0.3621941411404279 1107 6 0.31367843644173377 1107 7 0.25063915859329455 1107 8 0.21754874110706623 1107 9 0.2991132428965386 1107 10-11 0.29228388893003654 1107 12-13 0.2748159785390101 1107 14-15 0.3879755440630106 1107 16-17 0.4568174646168899 1107 18-19 0.4566208631015414 1107 20-21 0.5077158888131308 1107 22-23 0.5108706266683498 1107 24-25 0.4886414142564135 1107 26-27 0.40099503388842805 1107 28-29 0.47198465873913875 1107 30-31 0.6715937390367159 1107 32-33 0.6172668598519948 1107 34-35 0.6505868380184197 1107 36-37 0.5514532051218453 1107 38-39 0.4879181199441902 1107 40-41 0.4369763082905038 1107 42-43 0.4781401041121427 1107 44-45 0.6580435838680856 1107 46-47 0.6196040204095752 1107 48-49 0.46790549879439425 1107 50-51 0.4835614324223769 1107 52-53 0.455003196826425 1107 54-55 0.43693481794042555 1107 56-57 0.5173853445495666 1107 58-59 0.6201788077834252 1107 60-61 0.6279798009414819 1107 62-63 0.7051480755193609 1107 64-65 0.5287495389507626 1107 66-67 0.6291965508542212 1107 68-69 0.4894201470939663 1107 70-71 0.5095037888714202 1107 72-73 0.563648056060785 1107 74-75 0.5564560494569726 1107 76-77 0.7378984834667897 1107 78-79 0.9073312384868828 1107 80-81 0.6043973991515053 1107 82-83 0.720815947225244 1107 84-85 0.6089020054102363 1107 86-87 0.5471344255931534 1107 88-89 0.6271640313684657 1107 90-91 0.7334125928121544 1107 92-93 0.6584206309702161 1107 94-95 0.6913081422871592 1107 96-97 0.7373753479988245 1107 98-99 0.6628278392049296 1107 100-101 0.31007108262097915 1108 1 0.35255424356297027 1108 2 0.3405194537082181 1108 3 0.40252733596447854 1108 4 0.2608481466474899 1108 5 0.42121084541668097 1108 6 0.2829424276776038 1108 7 0.33158677982570595 1108 8 0.3315582355779867 1108 9 0.24927827253164736 1108 10-11 0.3753092449726978 1108 12-13 0.2929389244467444 1108 14-15 0.4422663145758463 1108 16-17 0.28951557040439724 1108 18-19 0.36426700211033136 1108 20-21 0.41515135169199624 1108 22-23 0.4292964780340043 1108 24-25 0.35217501475829494 1108 26-27 0.3620376232385141 1108 28-29 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64-65 -0.7935402622617431 2316 66-67 -0.8188485761629387 2316 68-69 -0.5557081156442258 2316 70-71 -0.8790569324547874 2316 72-73 -0.6513564173517707 2316 74-75 -0.554574678889562 2316 76-77 -0.4649188767429244 2316 78-79 -0.5142396731094223 2316 80-81 -0.6587830346568175 2316 82-83 -0.392974275230646 2316 84-85 -0.49152053100401005 2316 86-87 -0.7002710307127167 2316 88-89 -0.7657507047476457 2316 90-91 -0.675822744276271 2316 92-93 -0.6414085110052952 2316 94-95 -0.8030771377536858 2316 96-97 -1.186469755909858 2316 98-99 -1.2868466254268753 2316 100-101 -1.1341632212289543 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9474.0 3 3367.0 4 3601.0 5 4032.0 6 4698.0 7 6040.0 8 7562.0 9 9558.0 10 11872.0 11 14683.0 12 17754.0 13 20632.0 14 23563.0 15 26801.0 16 30362.0 17 34446.0 18 33712.0 19 32541.0 20 34406.0 21 37061.0 22 41257.0 23 46029.0 24 51393.0 25 57407.0 26 64712.0 27 73425.0 28 83761.0 29 95319.0 30 110185.0 31 128725.0 32 152187.0 33 181566.0 34 221281.0 35 272216.0 36 325257.0 37 323537.0 38 154535.0 39 8355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.60783997418938 18.342975981304743 14.171894914756667 23.877289129749204 2 15.580287668345052 19.532180632876166 40.184889737777766 24.702641961001024 3 19.453041604773162 23.35543478813009 29.18495049849823 28.00657310859852 4 13.072281287419719 15.979026815196487 36.34456983199681 34.60412206538699 5 14.109803192952484 36.91186321848507 33.974744655974135 15.003588932588308 6 33.26646332280324 35.2737974920117 17.09481981205511 14.364919373129942 7 29.50907814515237 31.95782821090974 20.131369621727323 18.401724022210573 8 26.657449343419902 35.045769778305356 19.39421797385289 18.90256290442185 9 26.46899185993693 16.744707652878336 19.796541960726262 36.98975852645847 10-11 25.315354146870295 26.200508165810234 27.189211036841836 21.294926650477635 12-13 26.099146891782166 23.42570078301359 27.543252231091685 22.93190009411256 14-15 22.731617964500252 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28.197814356323402 22.86373735999637 40-41 22.789793226185534 26.345056398876977 28.298870426325777 22.56627994861172 42-43 22.63778545713679 26.23993807179167 28.14417969337339 22.978096777698156 44-45 22.599208871462196 26.59483777113779 28.16842663418928 22.637526723210737 46-47 22.836084241612753 26.81539362462475 27.590330571452977 22.75819156230952 48-49 22.729752345192622 26.779156358144274 27.589450650212203 22.9016406464509 50-51 22.82828268173668 26.929695531859448 27.57209467312687 22.669927113277005 52-53 23.024296100059917 26.76555311657166 27.55272992904056 22.65742085432786 54-55 22.609116550881282 26.872837014907837 27.673768303367325 22.844278130843563 56-57 22.627999191178404 27.125845204966453 27.52356418298591 22.72259142086923 58-59 22.67023449946818 26.613554198021976 27.593698682555672 23.122512619954172 60-61 22.481154518980045 26.850172305167852 27.885925672835267 22.782747503016836 62-63 22.47394319775787 26.882296244646962 27.907884727155018 22.735875830440147 64-65 22.27722691478865 26.78033765804656 27.81733670615386 23.125098721010932 66-67 22.243590441061798 26.926421999049765 27.713993493422702 23.11599406646574 68-69 22.607211887038748 26.55549135736377 27.880984531862797 22.956312223734688 70-71 22.422465705819587 26.70057073685398 27.614326479908314 23.262637077418123 72-73 22.45277468238353 26.75735567240669 27.554280640050145 23.235589005159635 74-75 22.424060612941734 27.057107753962878 27.39970059174302 23.119131041352365 76-77 22.458533446548962 26.769226918865236 27.513591860081732 23.25864777450407 78-79 22.608025782780942 26.827631330315043 27.504552644015334 23.05979024288868 80-81 22.59336990143948 26.97106075737581 27.33821677801097 23.09735256317374 82-83 22.559372137571042 26.97856801797644 27.3561842247715 23.105875619681015 84-85 22.585631563895735 26.845099269161505 27.332028737379975 23.23724042956279 86-87 22.65855526436864 26.78552678324178 27.29330549980568 23.262612452583895 88-89 22.58335194073541 26.863513934873307 27.142652174236385 23.410481950154896 90-91 22.449454193999898 26.859148003155635 27.286744561123072 23.404653241721395 92-93 22.67059937817113 26.430610870143255 27.389744358032974 23.509045393652645 94-95 22.423400652940572 26.39021960468762 27.481386404919654 23.704993337452155 96-97 22.669324038939802 26.079531787790916 27.46471157400874 23.786432599260547 98-99 22.50760678886862 26.21374895347707 27.18565498188445 24.092989275769856 100-101 22.99812290974226 25.675304961353433 27.448636917294557 23.877935211609746 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 396.0 1 288.0 2 259.5 3 390.5 4 351.5 5 313.0 6 554.5 7 1173.0 8 1923.5 9 2238.5 10 1979.0 11 1729.0 12 1835.5 13 1947.5 14 2055.5 15 2206.5 16 2427.5 17 2651.5 18 2872.0 19 3233.0 20 3668.0 21 4352.0 22 5315.0 23 6562.0 24 8083.5 25 10076.5 26 12943.5 27 16589.5 28 19814.0 29 23188.0 30 27537.5 31 33576.5 32 41307.0 33 45754.5 34 48172.5 35 52577.0 36 58681.5 37 65157.0 38 70256.5 39 74393.5 40 79073.0 41 85104.0 42 89815.5 43 94038.5 44 98559.0 45 103908.0 46 106416.5 47 107051.5 48 108749.0 49 107111.0 50 104659.0 51 102051.0 52 98802.0 53 97506.5 54 97012.0 55 91021.5 56 82171.0 57 75452.0 58 69470.0 59 60748.0 60 49670.0 61 41350.5 62 34815.5 63 26977.5 64 20224.5 65 15944.0 66 12234.5 67 9612.5 68 7548.5 69 5585.0 70 4202.0 71 3082.5 72 2321.5 73 1808.0 74 1380.5 75 1073.5 76 767.0 77 493.5 78 314.0 79 205.0 80 130.0 81 75.0 82 43.5 83 29.0 84 21.0 85 14.5 86 6.0 87 5.0 88 6.0 89 4.0 90 4.0 91 3.5 92 2.0 93 2.0 94 1.5 95 0.5 96 0.5 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1797402687835109 2 0.046820961864308425 3 0.044753731170067076 4 0.05120929368892603 5 0.058680337952324586 6 0.06227079126337534 7 0.03507038739177866 8 0.03293062228721305 9 0.03873337511315368 10-11 0.04080060580739503 12-13 0.03764535895828981 14-15 0.03256795023559177 16-17 0.03131673165749832 18-19 0.039204848780261356 20-21 0.04919646380242787 22-23 0.04620441937655224 24-25 0.06611511501056101 26-27 0.05159009934312837 28-29 0.04328490936100086 30-31 0.05240611145927628 32-33 0.048579921314671676 34-35 0.04780017640368591 36-37 0.04564227769653924 38-39 0.024915569946382565 40-41 0.023664351368289118 42-43 0.020726707750156673 44-45 0.004043793375577373 46-47 0.011369768818327413 48-49 0.010481222291855257 50-51 0.00817825476406007 52-53 0.008849198059559455 54-55 0.009846546201518001 56-57 0.007779315507276652 58-59 0.009774011791193743 60-61 0.010336153471206741 62-63 0.004152594991063761 64-65 0.006292360095629366 66-67 0.0036992549265371494 68-69 0.008160121161479005 70-71 0.02221366316180396 72-73 0.019149084325604068 74-75 0.019620557992711743 76-77 0.01200444490866467 78-79 0.004460866234941856 80-81 0.006292360095629366 82-83 0.005349412761414015 84-85 0.004950473504630596 86-87 0.007452910660817491 88-89 0.028741760090987167 90-91 0.03537865863565676 92-93 0.021941659123087993 94-95 0.03115352923426874 96-97 0.034635180929833115 98-99 0.02045470371144071 100-101 0.12024391871503842 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2757312.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.364179388220485 #Duplication Level Percentage of deduplicated Percentage of total 1 78.47280773526988 45.01528220023357 2 12.350692079446114 14.169746320280415 3 3.943296323897638 6.786118731149235 4 1.737095094993208 3.985881385743531 5 0.9227095080698754 2.6465236872068503 6 0.5758354731301797 1.9819397627244266 7 0.3666469844458728 1.4722682371532192 8 0.265752404003399 1.2195734860881458 9 0.18861366703819354 0.9737701407944119 >10 1.0477785287548878 10.998904258709825 >50 0.08059312028929143 3.191809702268046 >100 0.04341316573785624 4.333422896906298 >500 0.002859548954225418 1.1483955637420797 >1k 0.001779274904804043 1.6145849639351335 >5k 1.2709106462886023E-4 0.4617786630648245 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7142 0.2590203792679247 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5526 0.20041257572592439 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3768 0.13665482905090173 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.6267205162128914E-5 0.0 5 0.0 0.0 0.0 3.6267205162128914E-5 0.0 6 0.0 0.0 0.0 3.6267205162128914E-5 0.0 7 0.0 0.0 0.0 3.6267205162128914E-5 0.0 8 0.0 0.0 0.0 3.6267205162128914E-5 0.0 9 0.0 0.0 0.0 7.253441032425783E-5 0.0 10-11 0.0 0.0 0.0 7.253441032425783E-5 0.0 12-13 0.0 0.0 0.0 7.253441032425783E-5 0.0 14-15 0.0 0.0 0.0 7.253441032425783E-5 0.0 16-17 0.0 0.0 0.0 7.253441032425783E-5 0.0 18-19 0.0 0.0 0.0 9.066801290532228E-5 0.0 20-21 0.0 0.0 0.0 2.1760323097277348E-4 0.0 22-23 0.0 0.0 0.0 3.445384490402247E-4 0.0 24-25 0.0 0.0 0.0 6.346760903372559E-4 0.0 26-27 0.0 0.0 0.0 0.0011968177703502543 0.0 28-29 0.0 0.0 0.0 0.0031008460413620223 0.0 30-31 0.0 0.0 0.0 0.009628942970545225 0.0 32-33 0.0 0.0 0.0 0.025713448459949398 0.0 34-35 0.0 0.0 0.0 0.049667937469535545 0.0 36-37 0.0 0.0 0.0 0.09037787526402526 0.0 38-39 0.0 0.0 0.0 0.1542988243622775 0.0 40-41 0.0 0.0 0.0 0.22463906877422649 0.0 42-43 0.0 0.0 0.0 0.2979532240094701 0.0 44-45 0.0 0.0 0.0 0.38802282802961724 0.0 46-47 0.0 0.0 0.0 0.4940318687185201 0.0 48-49 0.0 0.0 0.0 0.612444293572871 0.0 50-51 0.0 0.0 0.0 0.734846110995056 0.0 52-53 0.0 0.0 0.0 0.8540382807603928 0.0 54-55 0.0 0.0 0.0 0.989713895271917 0.0 56-57 0.0 0.0 0.0 1.1515744319104984 0.0 58-59 3.6267205162128914E-5 0.0 0.0 1.3283589234732958 0.0 60-61 3.6267205162128914E-5 0.0 0.0 1.5206839124480653 0.0 62-63 3.6267205162128914E-5 0.0 0.0 1.7075506870459347 0.0 64-65 3.6267205162128914E-5 0.0 0.0 1.9040101374091871 0.0 66-67 3.6267205162128914E-5 0.0 0.0 2.13336394285449 0.0 68-69 3.6267205162128914E-5 0.0 0.0 2.3931096662256577 0.0 70-71 3.6267205162128914E-5 0.0 0.0 2.6692300327275262 0.0 72-73 3.6267205162128914E-5 0.0 0.0 2.9484875124759187 0.0 74-75 3.6267205162128914E-5 0.0 0.0 3.2247892150035975 0.0 76-77 3.6267205162128914E-5 0.0 0.0 3.5290166655061164 0.0 78-79 3.6267205162128914E-5 0.0 0.0 3.8737908513798947 0.0 80-81 3.6267205162128914E-5 0.0 0.0 4.2417397813522735 0.0 82-83 3.6267205162128914E-5 0.0 0.0 4.623361447670775 0.0 84-85 3.6267205162128914E-5 0.0 0.0 5.007322348722234 0.0 86-87 3.6267205162128914E-5 0.0 0.0 5.4070957512243805 0.0 88-89 3.6267205162128914E-5 0.0 0.0 5.838874962282107 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4580 0.0 45.31197 1 GTATCAA 7595 0.0 40.359898 1 ATCAACG 9535 0.0 31.699314 3 TCAACGC 9640 0.0 31.255445 4 TATCAAC 9705 0.0 31.241982 2 CAACGCA 9735 0.0 30.94931 5 AACGCAG 10195 0.0 29.64072 6 GTACATG 8900 0.0 25.831467 1 ACGCAGA 11750 0.0 25.593027 7 CGCAGAG 11870 0.0 25.214226 8 TACATGG 9110 0.0 24.987947 2 ACATGGG 8900 0.0 24.883379 3 GCAGAGT 13090 0.0 22.4271 9 AGGTAAG 1385 0.0 21.960573 2 CATGGGG 5460 0.0 21.847137 4 GTAAGGT 1420 0.0 21.753967 4 GGTAAGG 1515 0.0 21.330927 3 GAGTACT 7880 0.0 20.585117 12-13 TAAGGTG 1680 0.0 20.08384 5 GTACTTT 7925 0.0 20.020523 14-15 >>END_MODULE