FastQCFastQC Report
Thu 26 May 2016
SRR938582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938582_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2757312
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68310.2477412784625026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57250.20762974955318803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42050.15250359770675206No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44100.051.226581
GTATCAA75750.039.805861
ATCAACG98850.030.2002493
TATCAAC102300.029.427992
TCAACGC101650.029.3678344
CAACGCA103400.029.0087155
AACGCAG108450.027.6343446
ACGCAGA121250.024.3645277
CGCAGAG123800.023.9010378
GTACATG94100.023.2492311
TACATGG95550.022.5475852
ACATGGG93800.021.9438363
GCAGAGT135950.021.3806829
GTGGTAT22100.020.6594771
TAAGGTG13400.020.2203985
AGAGTAC124850.020.08605810-11
GAGTACT79550.019.941412-13
AGTACTT83550.019.38461912-13
GTACTTT81950.019.18352314-15
CAGAGTA131350.018.96551910-11
Produced by FastQC (version 0.11.5)