FastQCFastQC Report
Thu 26 May 2016
SRR938581_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938581_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755373
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73200.2656627614482685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55960.20309410014542495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38360.13921890067152431No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27680.10045826826349825No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44750.045.811141
GTATCAA74600.041.92381
ATCAACG93600.032.698133
TATCAAC96650.032.1088142
TCAACGC96100.031.8969574
CAACGCA97500.031.5364345
AACGCAG101900.030.2185976
ACGCAGA117600.025.8958157
CGCAGAG119400.025.4258448
GTACATG88150.024.8248141
TACATGG89750.023.245722
TAAGGTG14500.022.942625
GTAAGGT13350.022.4270534
ACATGGG90100.022.3114873
GCAGAGT131400.022.2710429
GTGGTAT22900.021.6517751
GAGTACT77800.020.7597912-13
GTACTTT80400.019.8528414-15
TGGTATC23350.019.5387732
AGAGTAC119350.019.3439610-11
Produced by FastQC (version 0.11.5)