FastQCFastQC Report
Thu 26 May 2016
SRR938581_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938581_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755373
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69570.2524885015567765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59130.21459889459612183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40460.14684037333602382No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45050.048.462781
GTATCAA74300.039.8191571
ATCAACG93600.030.9322093
TCAACGC96450.029.9689044
TATCAAC97250.029.7864062
CAACGCA98100.029.3679165
AACGCAG103750.027.7876746
GTACATG90900.025.2216591
TACATGG92450.024.2328472
ACGCAGA119850.023.9359427
CGCAGAG121350.023.6009318
GTGGTAT24400.023.1978421
ACATGGG93200.023.0564293
TAAGGTG16050.022.2154835
TGGTATC25050.022.0262582
CTATACG1100.00286395421.6096084
GCAGAGT134450.021.0187959
GTAAGGT14950.020.3520574
GAGTACT78750.020.14408312-13
AGAGTAC122300.019.45641910-11
Produced by FastQC (version 0.11.5)