##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938581_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2755373 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78467815428256 31.0 31.0 34.0 30.0 34.0 2 32.01664928849923 31.0 31.0 34.0 30.0 34.0 3 32.08640064339746 33.0 31.0 34.0 30.0 34.0 4 35.76099497236853 37.0 35.0 37.0 35.0 37.0 5 35.62956449090559 37.0 35.0 37.0 33.0 37.0 6 35.66855812262079 37.0 35.0 37.0 33.0 37.0 7 35.601965686678355 37.0 35.0 37.0 33.0 37.0 8 35.58627271153488 37.0 35.0 37.0 33.0 37.0 9 37.14998332349195 39.0 37.0 39.0 33.0 39.0 10-11 37.078779170732965 39.0 37.0 39.0 33.0 39.0 12-13 36.96742600729556 39.0 37.0 39.0 33.0 39.0 14-15 38.02098971718166 40.0 37.0 41.0 33.0 41.0 16-17 37.938164270318396 40.0 37.0 41.0 33.0 41.0 18-19 37.92759655407816 40.0 37.0 41.0 33.0 41.0 20-21 37.91243399713941 40.0 37.0 41.0 32.5 41.0 22-23 37.94801266471001 40.0 37.0 41.0 33.0 41.0 24-25 37.875489452789154 40.0 37.0 41.0 32.0 41.0 26-27 37.70657058046225 40.0 37.0 41.0 32.0 41.0 28-29 37.57114009609588 39.5 37.0 41.0 32.0 41.0 30-31 37.329800901729094 39.0 36.0 41.0 31.5 41.0 32-33 37.11712878800801 39.0 36.0 41.0 31.0 41.0 34-35 36.952715657734906 39.0 36.0 40.5 30.5 41.0 36-37 36.97350213564552 39.0 36.0 41.0 31.0 41.0 38-39 36.82547299403747 39.0 35.0 41.0 30.0 41.0 40-41 36.6497446988121 39.0 35.0 40.5 30.0 41.0 42-43 36.43343569092097 39.0 35.0 40.0 30.0 41.0 44-45 36.165711865507866 38.5 35.0 40.0 30.0 41.0 46-47 35.81678161178178 38.0 35.0 40.0 28.5 41.0 48-49 35.440969335186196 38.0 34.0 40.0 27.5 41.0 50-51 35.07293132363567 38.0 34.0 40.0 26.5 41.0 52-53 34.548828416334196 38.0 33.0 40.0 24.5 41.0 54-55 34.22388094098331 37.5 33.0 40.0 24.0 41.0 56-57 33.852828636993976 37.0 33.0 40.0 23.0 41.0 58-59 33.38346840881434 36.5 32.0 39.5 22.5 41.0 60-61 32.969321939352675 36.0 31.0 39.0 22.0 40.0 62-63 32.47672583711897 35.5 31.0 39.0 20.0 40.0 64-65 31.971180489901005 35.0 30.5 38.5 18.0 40.0 66-67 31.468495372495845 35.0 30.0 38.0 16.5 40.0 68-69 30.89697456569401 34.5 29.0 37.5 12.5 40.0 70-71 30.313073039475963 34.0 29.0 37.0 8.0 39.0 72-73 29.70750021866368 34.0 28.0 36.0 7.0 39.0 74-75 29.07501597787305 33.5 27.5 36.0 4.0 38.5 76-77 27.812959443240537 31.5 26.0 34.5 2.0 36.5 78-79 28.428973681603182 33.0 27.0 35.0 2.0 37.0 80-81 28.10965774869682 33.0 26.0 35.0 2.0 37.0 82-83 27.600349027155307 33.0 26.0 35.0 2.0 36.0 84-85 26.9872935533592 32.0 25.5 35.0 2.0 36.0 86-87 26.325533602891515 32.0 24.5 34.5 2.0 35.0 88-89 25.557633939216217 31.0 21.0 34.0 2.0 35.0 90-91 24.991723988004527 31.0 19.5 34.0 2.0 35.0 92-93 24.192811644739205 31.0 15.0 34.0 2.0 35.0 94-95 23.378405210474227 30.5 4.5 34.0 2.0 35.0 96-97 22.373001041964194 30.0 2.0 34.0 2.0 35.0 98-99 21.169575589221495 29.0 2.0 34.0 2.0 35.0 100-101 18.04906776686859 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31206123520972895 1101 2 0.332758400968757 1101 3 0.2697852088935164 1101 4 0.15735455101104634 1101 5 0.2484517481480637 1101 6 0.25764013829962096 1101 7 0.2817501782144092 1101 8 0.2564329720925187 1101 9 0.2624747275889021 1101 10-11 0.32958403563939953 1101 12-13 0.3690908691960928 1101 14-15 0.5235780652811997 1101 16-17 0.48818840733553515 1101 18-19 0.596656458950946 1101 20-21 0.5742902927207325 1101 22-23 0.5156385937987906 1101 24-25 0.5840440236465412 1101 26-27 0.4667740954570405 1101 28-29 0.4601264425353335 1101 30-31 0.5233093652883198 1101 32-33 0.43661003425755496 1101 34-35 0.33982294860418705 1101 36-37 0.6248333799026682 1101 38-39 0.8162005741289704 1101 40-41 0.8482809145522481 1101 42-43 0.8666304636702691 1101 44-45 0.8510122644178395 1101 46-47 0.8956133895214933 1101 48-49 0.9247599380086626 1101 50-51 1.0521419778107841 1101 52-53 0.9903612107073272 1101 54-55 1.1525100289912231 1101 56-57 1.1249729361609866 1101 58-59 1.1029076055607447 1101 60-61 1.2922739212059398 1101 62-63 1.3448653481346327 1101 64-65 1.3945825670315877 1101 66-67 1.2888139382309909 1101 68-69 1.5164516380392428 1101 70-71 1.5403464786368346 1101 72-73 1.710040289605427 1101 74-75 1.6381734495441904 1101 76-77 1.6203546164566625 1101 78-79 1.8741755218372909 1101 80-81 2.2123243322012414 1101 82-83 2.3767719205134483 1101 84-85 2.669031614853779 1101 86-87 2.708985805175196 1101 88-89 2.911444125417333 1101 90-91 2.977999729656318 1101 92-93 3.1872400345778225 1101 94-95 3.3804469630605674 1101 96-97 3.6868384892140504 1101 98-99 3.831341295890457 1101 100-101 4.430213767563476 1102 1 0.28382200047737527 1102 2 0.31906386288255817 1102 3 0.23842929401613588 1102 4 0.20710614796887228 1102 5 0.10680522329185749 1102 6 0.12275285671711345 1102 7 0.2230494840034325 1102 8 0.30040894276119445 1102 9 0.39391026319447064 1102 10-11 0.3740623211018601 1102 12-13 0.37201928777622584 1102 14-15 0.4582422157359787 1102 16-17 0.4357437897421903 1102 18-19 0.503309389811534 1102 20-21 0.4793167345260443 1102 22-23 0.4886652264212543 1102 24-25 0.45063188358675177 1102 26-27 0.496636695749622 1102 28-29 0.40518672031058856 1102 30-31 0.37760371664630554 1102 32-33 0.2630746126879515 1102 34-35 0.47114035930629683 1102 36-37 0.5655957284983444 1102 38-39 0.663629767335884 1102 40-41 0.7066088338275023 1102 42-43 0.7702087395918653 1102 44-45 0.7200662656717824 1102 46-47 0.7542812018482792 1102 48-49 0.8911386373626158 1102 50-51 0.9225282916777431 1102 52-53 1.0441810179819981 1102 54-55 1.0103850414852005 1102 56-57 1.0565666909882054 1102 58-59 1.1238963564353242 1102 60-61 1.2432481109145357 1102 62-63 1.4907163812732094 1102 64-65 1.3318386504641175 1102 66-67 1.455512881013103 1102 68-69 1.5046879877979933 1102 70-71 1.576774665510431 1102 72-73 1.6264285866077834 1102 74-75 1.7705199098860263 1102 76-77 1.7739805966661955 1102 78-79 2.121928309130684 1102 80-81 2.25353941720574 1102 82-83 2.236991541968564 1102 84-85 2.402015665720178 1102 86-87 2.4708210118483436 1102 88-89 2.4390870565505125 1102 90-91 2.766775081077906 1102 92-93 2.9770217193115442 1102 94-95 3.250585385002722 1102 96-97 3.646874221997347 1102 98-99 4.0609758242540615 1102 100-101 4.5799163540445385 1103 1 0.19940345802188375 1103 2 0.22242263828949405 1103 3 0.28534667127052415 1103 4 0.2137136705071896 1103 5 0.1108628974172916 1103 6 0.10769401961000824 1103 7 0.21212461395110438 1103 8 0.22621753684064316 1103 9 0.24968888386269583 1103 10-11 0.3070719967098725 1103 12-13 0.39690090889278196 1103 14-15 0.44121460490870845 1103 16-17 0.4903897794529115 1103 18-19 0.5148443258697384 1103 20-21 0.569011695974531 1103 22-23 0.3959046763575529 1103 24-25 0.3659649036104611 1103 26-27 0.385347090009283 1103 28-29 0.4225519620287841 1103 30-31 0.452617282916286 1103 32-33 0.5517305754103887 1103 34-35 0.4147196573447687 1103 36-37 0.5280894308638153 1103 38-39 0.7107882302469761 1103 40-41 0.6773830933144609 1103 42-43 0.6826710647562138 1103 44-45 0.5956272267740417 1103 46-47 0.6715517848483259 1103 48-49 0.8819624012507035 1103 50-51 0.8203723367233096 1103 52-53 0.9499918481378486 1103 54-55 0.9019335327162352 1103 56-57 0.9470175350376877 1103 58-59 1.145584078100967 1103 60-61 1.0812965322334946 1103 62-63 1.1112485286167768 1103 64-65 1.152125106832326 1103 66-67 1.2256191196107729 1103 68-69 1.3342506933727662 1103 70-71 1.3783626958988755 1103 72-73 1.4647099094328517 1103 74-75 1.49333410358906 1103 76-77 1.4472870600921972 1103 78-79 1.7084325615598495 1103 80-81 2.092861930444645 1103 82-83 2.2800379547027703 1103 84-85 2.254599832079826 1103 86-87 2.4250374862383346 1103 88-89 2.6783541672512747 1103 90-91 2.900366913351089 1103 92-93 3.12053829069065 1103 94-95 3.249718558611029 1103 96-97 3.5896714481528917 1103 98-99 3.6476061680882594 1103 100-101 3.84732653923383 1104 1 0.2541571921629675 1104 2 0.2663911909849759 1104 3 0.23043450772664897 1104 4 0.169233617168409 1104 5 0.200888608246089 1104 6 0.2503166945586699 1104 7 0.21891551057098724 1104 8 0.24121268011407437 1104 9 0.3358001902369381 1104 10-11 0.2964503037572399 1104 12-13 0.3466218068759588 1104 14-15 0.552139550377035 1104 16-17 0.6201174992197878 1104 18-19 0.5401763885726325 1104 20-21 0.5168203910061848 1104 22-23 0.5172072802446408 1104 24-25 0.5433688235000318 1104 26-27 0.5423781869052391 1104 28-29 0.6315400481377011 1104 30-31 0.5704819687627634 1104 32-33 0.6875317329302248 1104 34-35 0.7487295774217912 1104 36-37 0.783938138505917 1104 38-39 0.6501620906203485 1104 40-41 0.7774488348599249 1104 42-43 0.8615164259433001 1104 44-45 0.9269284034651619 1104 46-47 0.9250807665120533 1104 48-49 0.9787016578733159 1104 50-51 0.9701206070470008 1104 52-53 1.1758992625273876 1104 54-55 1.1777916076631954 1104 56-57 1.4025944630303329 1104 58-59 1.514846137906865 1104 60-61 1.4691422278530766 1104 62-63 1.648222489745919 1104 64-65 1.5454201082382149 1104 66-67 1.723660852311884 1104 68-69 1.7655735442712555 1104 70-71 1.6263113679675847 1104 72-73 1.8878744448912137 1104 74-75 2.1554268202033207 1104 76-77 2.075677848713827 1104 78-79 2.3718938951999284 1104 80-81 2.477278361730484 1104 82-83 2.6923351650671954 1104 84-85 2.878950804064182 1104 86-87 3.1955988269078794 1104 88-89 3.2418995350418065 1104 90-91 3.2755925753996067 1104 92-93 3.7131410754269005 1104 94-95 3.9143655832269637 1104 96-97 4.013346019781089 1104 98-99 4.295704642479677 1104 100-101 4.634444151897103 1105 1 0.22713023433040647 1105 2 0.2078612858555715 1105 3 0.14741735851436744 1105 4 0.08816912892072537 1105 5 -0.0026720089862806162 1105 6 0.15542663958616032 1105 7 0.1975040851361669 1105 8 0.12991484542486376 1105 9 0.22152356830200404 1105 10-11 0.22509382011381973 1105 12-13 0.24418199194735735 1105 14-15 0.3660767160155345 1105 16-17 0.3511760222123925 1105 18-19 0.3263431156599097 1105 20-21 0.2317921827742353 1105 22-23 0.28336467285981115 1105 24-25 0.21276491438901246 1105 26-27 0.3781028706699203 1105 28-29 0.41369994155164846 1105 30-31 0.40242421997322 1105 32-33 0.5449618805613241 1105 34-35 0.4523360221502486 1105 36-37 0.5307976749262266 1105 38-39 0.6198676835206172 1105 40-41 0.5969531452047505 1105 42-43 0.6389971783949377 1105 44-45 0.6684777107787241 1105 46-47 0.7176244280829067 1105 48-49 0.7909480705276479 1105 50-51 0.8403466108483855 1105 52-53 0.9443893771012526 1105 54-55 0.9346449944943629 1105 56-57 0.8817554972673065 1105 58-59 1.1718266896391967 1105 60-61 1.1425205745403844 1105 62-63 1.1771332392891836 1105 64-65 1.2908386387270774 1105 66-67 1.4232612314256023 1105 68-69 1.4548423832124442 1105 70-71 1.3405874266595497 1105 72-73 1.5745688794362387 1105 74-75 1.6692185923500347 1105 76-77 1.5628099230455135 1105 78-79 1.7066102460408992 1105 80-81 2.1073958081302315 1105 82-83 2.292499734421863 1105 84-85 2.4742572748042235 1105 86-87 2.5996766291952618 1105 88-89 2.7536426388997164 1105 90-91 2.8038621240269315 1105 92-93 3.1617335056462146 1105 94-95 3.325680161593521 1105 96-97 3.4808476685468683 1105 98-99 3.9038762226731905 1105 100-101 4.299025667491765 1106 1 0.12691050499699585 1106 2 0.0838352754713938 1106 3 0.06084470735082448 1106 4 0.1309911274790707 1106 5 0.039625887695535766 1106 6 0.04236162220100681 1106 7 0.048527243982412926 1106 8 0.011580813457172212 1106 9 0.07205238659098967 1106 10-11 0.09202541296484412 1106 12-13 -0.025020364342047685 1106 14-15 0.017447451760745025 1106 16-17 -0.06271868022233917 1106 18-19 0.21688489273350342 1106 20-21 0.3589174057949762 1106 22-23 0.18797838354649343 1106 24-25 0.12032739172122575 1106 26-27 0.053554044703517434 1106 28-29 0.3112529053502797 1106 30-31 0.22799754152308793 1106 32-33 0.1921295866042314 1106 34-35 0.22962816077343007 1106 36-37 0.356267966824241 1106 38-39 0.19508172541547708 1106 40-41 0.23283424923872076 1106 42-43 0.3188068724132691 1106 44-45 -0.05262720089826445 1106 46-47 -0.2646693524571262 1106 48-49 0.3060815752660062 1106 50-51 0.12292050088989726 1106 52-53 0.25860686374270614 1106 54-55 0.3838439357984882 1106 56-57 0.31451761504243336 1106 58-59 0.7867124440054098 1106 60-61 0.5933136380343953 1106 62-63 0.7931299988363847 1106 64-65 0.5082018211503403 1106 66-67 0.4743895385261041 1106 68-69 0.5373471916832173 1106 70-71 0.7513815292775909 1106 72-73 1.2293354321523644 1106 74-75 1.3468375506130634 1106 76-77 1.3359797406288045 1106 78-79 1.5863632926667997 1106 80-81 1.6143754680340194 1106 82-83 1.9479099387207093 1106 84-85 1.8602455700304574 1106 86-87 2.213925037343003 1106 88-89 2.5011150705861347 1106 90-91 2.2924751406191213 1106 92-93 1.9781239469157832 1106 94-95 2.4442382862066836 1106 96-97 3.000945008238027 1106 98-99 3.370195362883809 1106 100-101 3.6319713470309587 1107 1 0.2578489978349374 1107 2 0.2325580829327052 1107 3 0.22760828796251786 1107 4 0.20502502264677247 1107 5 0.09706084692989236 1107 6 0.11537186382443565 1107 7 0.12011648488697801 1107 8 0.19260952106368023 1107 9 0.18708948161486916 1107 10-11 0.2558041652123393 1107 12-13 0.28696573432168293 1107 14-15 0.44860796233248124 1107 16-17 0.4745645989966718 1107 18-19 0.3948927926737298 1107 20-21 0.4015016355217256 1107 22-23 0.347818795353227 1107 24-25 0.3902936632455152 1107 26-27 0.32875978695167163 1107 28-29 0.3165711792256971 1107 30-31 0.3125675573661013 1107 32-33 0.48373341063467734 1107 34-35 0.6463564283663956 1107 36-37 0.584624962908677 1107 38-39 0.7027384722130208 1107 40-41 0.6769771096005073 1107 42-43 0.7111274840953286 1107 44-45 0.5968090143408276 1107 46-47 0.6650627071362294 1107 48-49 0.7468833273732116 1107 50-51 0.8137921521799498 1107 52-53 0.8866615610041393 1107 54-55 0.818078132707754 1107 56-57 0.8112892298201615 1107 58-59 0.8877924772371983 1107 60-61 0.9019477861959473 1107 62-63 1.08628741397672 1107 64-65 1.1163840111292949 1107 66-67 1.282704718666107 1107 68-69 1.333064528110043 1107 70-71 1.3403559204944706 1107 72-73 1.5061861674748052 1107 74-75 1.4941395996403735 1107 76-77 1.3700242163764536 1107 78-79 1.73208855180863 1107 80-81 1.966191078108512 1107 82-83 2.053442259157947 1107 84-85 2.2863907233761793 1107 86-87 2.4878928250859573 1107 88-89 2.702565363544881 1107 90-91 2.863248366106614 1107 92-93 3.1348801935646797 1107 94-95 3.2263598075714874 1107 96-97 3.6098092301050606 1107 98-99 4.020044613242334 1107 100-101 4.262463127153573 1108 1 0.17480280975664897 1108 2 0.15842461506240824 1108 3 0.15033986400104027 1108 4 0.14431828200832797 1108 5 0.09882299373651904 1108 6 0.16387700676582995 1108 7 0.09745482708404296 1108 8 0.15716614558979813 1108 9 0.26920224888253586 1108 10-11 0.16005798972333452 1108 12-13 0.29439094024012746 1108 14-15 0.3872264170959028 1108 16-17 0.4438435602733719 1108 18-19 0.4063616444354139 1108 20-21 0.3804479087101811 1108 22-23 0.25394040362103 1108 24-25 0.28888166112146507 1108 26-27 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1.1033336648715952 2316 82-83 1.4416487704596221 2316 84-85 1.4863987950077338 2316 86-87 1.7520258344406976 2316 88-89 1.8900373563750428 2316 90-91 2.0431316336144008 2316 92-93 1.9355095024938542 2316 94-95 2.621500963707458 2316 96-97 2.7154320956455926 2316 98-99 2.8938251706302403 2316 100-101 3.4557383447397108 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 17.0 4 34.0 5 91.0 6 191.0 7 433.0 8 1169.0 9 2179.0 10 3367.0 11 5186.0 12 7684.0 13 10738.0 14 14093.0 15 18478.0 16 23115.0 17 28089.0 18 27810.0 19 25603.0 20 26531.0 21 28270.0 22 31003.0 23 35295.0 24 40871.0 25 47647.0 26 55350.0 27 66202.0 28 78006.0 29 92821.0 30 109331.0 31 128224.0 32 152497.0 33 184635.0 34 228023.0 35 287100.0 36 361843.0 37 403396.0 38 217766.0 39 12282.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.24643432771848 19.173969675952893 14.774416947844774 23.80517904848385 2 16.004253868366085 20.363779087858212 39.24102797219809 24.390939071577616 3 19.49963162842014 23.653229874753663 29.71553729626148 27.131601200564713 4 13.497628088828629 16.774280650931832 35.96525769832251 33.76283356191703 5 13.97262802096639 37.09992312711346 34.14474917496065 14.782699676959496 6 33.22900384085929 35.820486010424 16.75889253469494 14.191617614021768 7 29.317119678533544 32.12849948083254 20.133825801443216 18.4205550391907 8 26.48835566001409 35.612347221229214 19.174500149344574 18.72479696941213 9 26.14480870648003 16.766949520083124 19.773402729866337 37.31483904357051 10-11 25.2641656864606 26.447562634895526 27.11001378034843 21.17825789829544 12-13 25.993159546819978 23.505982674577997 27.600818473578713 22.900039305023313 14-15 22.512251517308183 25.207803081470274 25.546994907767477 26.732950493454062 16-17 22.459572624105704 28.455602925629307 26.494416545418716 22.590407904846277 18-19 22.450880515995475 26.925519702777084 28.60547374166764 22.0181260395598 20-21 22.83723474099514 26.10773568587629 28.85781707231653 22.19721250081205 22-23 22.629622196341476 26.075017792509396 28.74064600328159 22.55471400786754 24-25 22.554915103159534 26.285955583041538 28.759857208392127 22.399272105406805 26-27 22.191751138173935 26.66448523468179 28.519936509803195 22.62382711734108 28-29 22.219514381537454 26.874419543197963 28.596291681743267 22.309774393521312 30-31 22.410813345416393 26.441084383130704 28.484510082663945 22.663592188788957 32-33 22.11362999589165 26.44349349124108 28.500571975245375 22.942304537621887 34-35 22.785652565854416 26.62761467092347 28.222393105960357 22.364339657261752 36-37 22.862178359978415 26.219988219038775 28.280313563748027 22.637519857234782 38-39 22.35368612013345 26.55737502606582 28.33767294865836 22.75126590514237 40-41 22.70984276174859 26.301384082793806 28.456087474439073 22.53268568101853 42-43 22.552530388930034 26.275656354250277 28.22717525179762 22.94463800502207 44-45 22.56436862456457 26.59855435084793 28.249280841083845 22.58779618350366 46-47 22.80554545911504 26.886846292777577 27.654964130971088 22.65264411713629 48-49 22.678036694124533 26.806824339209246 27.68100725382734 22.83413171283888 50-51 22.83670849645402 26.951868222560066 27.56556008932366 22.645863191662254 52-53 23.061596379147215 26.80141672289015 27.51175612158499 22.625230776377645 54-55 22.604239535670768 26.86887163169408 27.727630293181775 22.799258539453376 56-57 22.631618296325033 27.102537478591827 27.517000420632705 22.748843804450434 58-59 22.655625935218207 26.679309842986775 27.63360895239955 23.03145526939547 60-61 22.43708927974543 26.96077808703214 27.812096583656732 22.790036049565703 62-63 22.45376578778989 26.946242849879127 27.826795138081124 22.773196224249855 64-65 22.228896051460183 26.865019726911747 27.698554787319175 23.207529434308892 66-67 22.20976370842795 27.002373912440703 27.587704287668398 23.200158091462953 68-69 22.562235346101744 26.672467465773618 27.736717515903052 23.028579672221582 70-71 22.343454044153006 26.840830624383706 27.52260764695016 23.29310768451313 72-73 22.408176175154605 26.893360840815838 27.44109097448384 23.25737200954572 74-75 22.393392836468966 27.19464841965135 27.148611095485077 23.263347648394607 76-77 22.396641035533122 26.903272261142142 27.46439411288417 23.235692590440568 78-79 22.48454202026368 27.00128077033491 27.458877618384154 23.05529959101726 80-81 22.48950413954994 27.106630248111692 27.265211376177156 23.138654236161216 82-83 22.47769701769177 27.1769202376904 27.294382671668814 23.051000072949016 84-85 22.46245363413979 27.044779802995002 27.264867925350057 23.227898637515153 86-87 22.50160325216117 27.07917398125892 27.174770273109967 23.244452493469947 88-89 22.4385754223297 27.23295701377419 26.883530384837467 23.444937179058645 90-91 22.264874612980062 27.224064900671387 27.12939449775691 23.381665988591646 92-93 22.424391224693036 26.809649382587008 27.24997169162159 23.515987701098364 94-95 22.199172308068636 26.74835675605444 27.333794734868928 23.718676201007995 96-97 22.375409790253443 26.54050830867545 27.297393129714198 23.78668877135691 98-99 22.166527000155696 26.764579605011736 27.018029863833316 24.05086353099925 100-101 22.239326286571735 26.5471551674548 27.28406552257957 23.929453023393897 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 460.0 1 322.0 2 262.5 3 362.0 4 319.0 5 288.0 6 555.5 7 1213.0 8 1917.5 9 2232.5 10 2038.0 11 1719.5 12 1712.0 13 1869.5 14 2057.5 15 2233.5 16 2436.5 17 2640.5 18 2876.0 19 3195.5 20 3617.5 21 4298.0 22 5221.5 23 6399.5 24 8006.5 25 10148.0 26 13043.5 27 16623.0 28 19991.5 29 23616.0 30 28069.5 31 34244.5 32 42489.5 33 46672.0 34 48324.0 35 52923.5 36 59136.5 37 65386.5 38 70669.0 39 74988.5 40 79435.0 41 85808.0 42 90678.0 43 94684.5 44 99753.5 45 104942.0 46 106658.5 47 107603.5 48 109296.5 49 107522.5 50 104913.5 51 102185.0 52 99277.5 53 98422.5 54 98352.0 55 91745.0 56 82002.0 57 74579.5 58 68075.5 59 59719.5 60 48574.0 61 39652.5 62 33343.5 63 25684.0 64 19016.5 65 14678.0 66 10890.0 67 8571.5 68 6692.5 69 4829.0 70 3601.0 71 2587.0 72 1953.5 73 1564.0 74 1203.0 75 925.5 76 632.5 77 398.5 78 242.0 79 138.0 80 83.0 81 48.5 82 26.5 83 14.0 84 7.0 85 4.5 86 4.5 87 3.0 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.258545394761436E-5 2 0.042607661467249626 3 1.0887818092142153E-4 4 0.0 5 0.10078490280626252 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001052489082240408 26-27 0.010960403546089768 28-29 0.0 30-31 0.0 32-33 3.266345427642646E-4 34-35 0.015007042603669266 36-37 0.002903418157904574 38-39 0.011922160810895657 40-41 0.00693191085199717 42-43 0.004881371777977065 44-45 0.00257678361514031 46-47 5.4439090460710766E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.814636348690359E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.1775636184284306E-4 68-69 1.9960999835593948E-4 70-71 0.0 72-73 5.4439090460710766E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.629272697380718E-5 82-83 8.710254473713723E-4 84-85 0.0041010781480402105 86-87 0.0013065381710570584 88-89 7.258545394761436E-5 90-91 8.710254473713723E-4 92-93 1.8146363486903588E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 6.351227220416256E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2755373.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.98065562819713 #Duplication Level Percentage of deduplicated Percentage of total 1 76.41980397409415 42.01610925474 2 13.506116608666085 14.851502922706874 3 4.360924195615097 7.192994142593586 4 1.8931103585797835 4.163377947649915 5 0.9977555996037805 2.7428628511460396 6 0.6049001355857511 1.9954683626453955 7 0.4189269735779739 1.6123015767357394 8 0.29691104033749005 1.3059490928804227 9 0.21136971230868593 1.0459120826407464 >10 1.1451330485991569 11.5188740522407 >50 0.09134647344629118 3.4552085063622444 >100 0.048941350603898245 4.80813272774083 >500 0.0025125014070750237 0.9841311371339322 >1k 0.002115790658560115 1.839320447142986 >5k 1.322369161600072E-4 0.46785489564065524 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6957 0.2524885015567765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5913 0.21459889459612183 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4046 0.14684037333602382 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.814636348690359E-5 0.0 22-23 0.0 0.0 0.0 2.3590272532974664E-4 0.0 24-25 0.0 0.0 0.0 3.6292726973807175E-4 0.0 26-27 0.0 0.0 0.0 8.528790838844686E-4 0.0 28-29 0.0 0.0 0.0 0.0027582472500093454 0.0 30-31 0.0 0.0 0.0 0.00945425537667677 0.0 32-33 0.0 0.0 0.0 0.024080224347121062 0.0 34-35 0.0 0.0 0.0 0.04661800779785532 0.0 36-37 0.0 0.0 0.0 0.08860869290655021 0.0 38-39 0.0 0.0 0.0 0.1537904305515079 0.0 40-41 0.0 0.0 0.0 0.2229643681635844 0.0 42-43 0.0 0.0 0.0 0.29400738121481196 0.0 44-45 0.0 0.0 0.0 0.38473919864932987 0.0 46-47 0.0 0.0 0.0 0.4913490841348885 0.0 48-49 0.0 0.0 0.0 0.6058526377372502 0.0 50-51 0.0 0.0 0.0 0.7310806921603719 0.0 52-53 0.0 0.0 0.0 0.8513184966245949 0.0 54-55 0.0 0.0 0.0 0.9881965164063087 0.0 56-57 0.0 0.0 0.0 1.1484651987226413 0.0 58-59 0.0 0.0 0.0 1.3217992627495443 0.0 60-61 0.0 0.0 0.0 1.5107029066482105 0.0 62-63 0.0 0.0 0.0 1.6978282069251605 0.0 64-65 0.0 0.0 0.0 1.8946436653041165 0.0 66-67 0.0 0.0 0.0 2.1256287261289124 0.0 68-69 0.0 0.0 0.0 2.382254598560703 0.0 70-71 0.0 0.0 0.0 2.656682053573146 0.0 72-73 0.0 0.0 0.0 2.9390576157928527 0.0 74-75 0.0 0.0 0.0 3.221650934374402 0.0 76-77 0.0 0.0 0.0 3.538704197217582 0.0 78-79 0.0 0.0 0.0 3.886043740720403 1.814636348690359E-5 80-81 0.0 0.0 0.0 4.257989753111467 3.629272697380718E-5 82-83 0.0 0.0 0.0 4.638736751793678 3.629272697380718E-5 84-85 0.0 0.0 0.0 5.025199854974263 3.629272697380718E-5 86-87 0.0 0.0 0.0 5.4303355661828725 3.629272697380718E-5 88-89 0.0 0.0 0.0 5.8643421416991455 3.629272697380718E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4505 0.0 48.46278 1 GTATCAA 7430 0.0 39.819157 1 ATCAACG 9360 0.0 30.932209 3 TCAACGC 9645 0.0 29.968904 4 TATCAAC 9725 0.0 29.786406 2 CAACGCA 9810 0.0 29.367916 5 AACGCAG 10375 0.0 27.787674 6 GTACATG 9090 0.0 25.221659 1 TACATGG 9245 0.0 24.232847 2 ACGCAGA 11985 0.0 23.935942 7 CGCAGAG 12135 0.0 23.600931 8 GTGGTAT 2440 0.0 23.197842 1 ACATGGG 9320 0.0 23.056429 3 TAAGGTG 1605 0.0 22.215483 5 TGGTATC 2505 0.0 22.026258 2 CTATACG 110 0.002863954 21.609608 4 GCAGAGT 13445 0.0 21.018795 9 GTAAGGT 1495 0.0 20.352057 4 GAGTACT 7875 0.0 20.144083 12-13 AGAGTAC 12230 0.0 19.456419 10-11 >>END_MODULE