FastQCFastQC Report
Thu 26 May 2016
SRR938580_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938580_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742687
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67390.24570794990460085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53020.1933140748470387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35150.12815899152910995No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45900.044.2449461
GTATCAA76500.040.0074041
ATCAACG96400.031.0109333
TATCAAC98800.030.5462552
TCAACGC98450.030.3169254
CAACGCA99600.029.966885
AACGCAG103700.028.7309996
GTACATG86600.025.2674561
GTGGTAT25050.025.120771
ACGCAGA118950.024.8419087
GGACCGA6800.024.458016
CGCAGAG120800.024.3041558
TAAGGTG13300.024.2995645
TACATGG88250.024.0732122
GTAAGGT12850.022.5614974
ACATGGG90600.022.4520913
GCAGAGT129500.022.045719
TGGTATC26100.022.0336322
GTATAGG7450.021.116621
AGGACCG9100.020.8910275
Produced by FastQC (version 0.11.5)