##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938580_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2742687 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.066883315522333 31.0 31.0 34.0 28.0 34.0 2 31.237769019942853 31.0 31.0 34.0 28.0 34.0 3 31.293327674648985 31.0 31.0 34.0 27.0 34.0 4 34.95673804557356 37.0 35.0 37.0 32.0 37.0 5 34.93745330765049 37.0 35.0 37.0 32.0 37.0 6 34.91367771823763 37.0 35.0 37.0 32.0 37.0 7 34.826056710080294 37.0 35.0 37.0 32.0 37.0 8 34.78100928031525 37.0 35.0 37.0 32.0 37.0 9 36.25605583137996 39.0 35.0 39.0 32.0 39.0 10-11 36.080345478722144 38.5 35.0 39.0 30.5 39.0 12-13 35.891628537999416 38.0 35.0 39.0 30.0 39.0 14-15 37.05744968346735 39.0 36.0 41.0 31.0 41.0 16-17 36.97624464621738 39.0 36.0 41.0 31.0 41.0 18-19 36.95676302108115 39.0 36.0 41.0 31.0 41.0 20-21 36.88044643810978 39.0 36.0 41.0 30.0 41.0 22-23 36.7157238503701 39.0 36.0 41.0 30.0 41.0 24-25 36.562589533548675 39.0 36.0 41.0 30.0 41.0 26-27 36.33629320443784 39.0 35.5 41.0 29.5 41.0 28-29 36.09166521735801 39.0 35.0 40.0 28.5 41.0 30-31 35.80814398434819 38.5 35.0 40.0 27.0 41.0 32-33 35.50694300880852 38.0 34.5 40.0 27.0 41.0 34-35 35.18646312904097 38.0 34.0 40.0 25.5 41.0 36-37 34.943550612957296 38.0 34.0 40.0 25.0 41.0 38-39 34.68393713901732 38.0 33.5 40.0 24.5 41.0 40-41 34.38910747015609 38.0 33.0 40.0 24.0 41.0 42-43 33.965010043070905 38.0 33.0 40.0 23.0 41.0 44-45 33.484261784155464 37.5 32.5 40.0 20.0 41.0 46-47 32.990200303570916 37.0 31.5 40.0 17.0 41.0 48-49 32.480255493973615 37.0 31.0 40.0 14.0 41.0 50-51 31.751739443837376 36.0 30.0 38.5 10.5 40.5 52-53 31.740171043943405 36.0 30.0 39.0 9.0 40.0 54-55 31.67996457488587 36.0 30.0 39.0 7.5 40.0 56-57 31.220320802191427 36.0 29.5 39.0 4.0 40.0 58-59 30.731226166164788 35.0 28.5 39.0 2.0 40.0 60-61 30.14930048525406 35.0 28.0 38.5 2.0 40.0 62-63 29.390173395651782 34.0 26.5 38.0 2.0 40.0 64-65 28.704141777752987 34.0 26.0 38.0 2.0 40.0 66-67 27.96349054777304 34.0 24.5 37.0 2.0 39.5 68-69 27.081366557685946 33.0 22.5 36.5 2.0 39.0 70-71 26.198564218228327 32.0 20.0 36.0 2.0 39.0 72-73 25.255246953079222 31.0 15.5 35.0 2.0 38.0 74-75 24.28314514197209 31.0 7.5 35.0 2.0 37.5 76-77 23.298827390803254 30.0 2.0 34.0 2.0 36.5 78-79 22.284592809897738 29.0 2.0 34.0 2.0 36.0 80-81 21.2869477997307 28.5 2.0 34.0 2.0 35.5 82-83 20.256155551107362 26.5 2.0 33.5 2.0 35.0 84-85 19.251052161621068 25.5 2.0 33.0 2.0 35.0 86-87 18.240205134599755 24.0 2.0 32.0 2.0 34.5 88-89 17.138675685559456 19.5 2.0 31.5 2.0 34.0 90-91 15.972385292233493 12.0 2.0 31.0 2.0 34.0 92-93 14.818706618728276 2.0 2.0 30.5 2.0 34.0 94-95 13.576504719641724 2.0 2.0 29.5 2.0 34.0 96-97 12.19052228708562 2.0 2.0 29.0 2.0 34.0 98-99 10.549194275540739 2.0 2.0 26.0 2.0 33.5 100-101 7.445880444979686 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5721052478576851 1101 2 0.4790565644531206 1101 3 0.5193430898574398 1101 4 0.40698630484912 1101 5 0.40666086581633465 1101 6 0.5053699264915608 1101 7 0.47574924739033975 1101 8 0.45780302215179347 1101 9 0.5169444307485804 1101 10-11 0.6773818301137595 1101 12-13 0.524659170287471 1101 14-15 0.648800469630217 1101 16-17 0.7073827825689136 1101 18-19 0.6318240061322058 1101 20-21 0.779348003361676 1101 22-23 0.8304572066544509 1101 24-25 0.8470966662442123 1101 26-27 0.8185453011887276 1101 28-29 0.8752957796073986 1101 30-31 0.9097961185375922 1101 32-33 1.1358705172055679 1101 34-35 1.196784944990739 1101 36-37 1.0745856729795946 1101 38-39 1.2417135084408386 1101 40-41 1.3216775169093822 1101 42-43 1.406441556404907 1101 44-45 1.3901198179140337 1101 46-47 1.4673545939706258 1101 48-49 1.4045555302818897 1101 50-51 1.6459772978018208 1101 52-53 1.7071847530835065 1101 54-55 1.930594153682506 1101 56-57 2.1624286153810637 1101 58-59 2.1618285850802472 1101 60-61 2.1433165551970994 1101 62-63 2.0259120923211107 1101 64-65 2.139938077500961 1101 66-67 2.108084546644889 1101 68-69 2.2489886966109047 1101 70-71 2.6853258258737682 1101 72-73 2.8573835971477806 1101 74-75 2.919670756499407 1101 76-77 3.0169685238612054 1101 78-79 3.0273336336944787 1101 80-81 3.566694958644103 1101 82-83 3.631562944206518 1101 84-85 3.763754340202457 1101 86-87 3.9387824360116603 1101 88-89 4.116884906787359 1101 90-91 4.1777598319895315 1101 92-93 4.07341955987077 1101 94-95 3.9023055114431653 1101 96-97 3.958040640270937 1101 98-99 3.669184534133601 1101 100-101 2.823991750520743 1102 1 0.6881483743174783 1102 2 0.488868459681683 1102 3 0.5179145780122631 1102 4 0.340305040251188 1102 5 0.40087382877929656 1102 6 0.42631170160000664 1102 7 0.42178903718012606 1102 8 0.5101261786416131 1102 9 0.5774007203715357 1102 10-11 0.7107798114284023 1102 12-13 0.680236622739919 1102 14-15 0.7899207565838395 1102 16-17 0.830792650770448 1102 18-19 0.7600303374635331 1102 20-21 0.8407635856105955 1102 22-23 0.6578210288099342 1102 24-25 0.732424702613919 1102 26-27 0.7954492885093813 1102 28-29 0.562383909403863 1102 30-31 0.5669584892416353 1102 32-33 0.6805766817033998 1102 34-35 0.8052684284742213 1102 36-37 0.9284082455521627 1102 38-39 1.0300695728385634 1102 40-41 1.1748692503094489 1102 42-43 1.3280454519671352 1102 44-45 1.4028617264892773 1102 46-47 1.1654693754187377 1102 48-49 1.3602612359875934 1102 50-51 1.3612498620327145 1102 52-53 1.6581878395032597 1102 54-55 1.553821547743226 1102 56-57 1.7591607641965545 1102 58-59 1.9255245727345702 1102 60-61 1.870079776751986 1102 62-63 2.022278250145604 1102 64-65 2.1037091402303574 1102 66-67 2.25963714090582 1102 68-69 2.302177301882846 1102 70-71 2.2953368785931545 1102 72-73 2.4345805858031007 1102 74-75 2.3881392249678797 1102 76-77 2.534293140144154 1102 78-79 3.0792136386994855 1102 80-81 3.478267990008799 1102 82-83 3.463014187116098 1102 84-85 3.486810729925512 1102 86-87 3.527073852428078 1102 88-89 3.7412853488961346 1102 90-91 3.885644800291166 1102 92-93 3.7710338408183848 1102 94-95 3.594570693291681 1102 96-97 3.6215739652625967 1102 98-99 3.2633099095089744 1102 100-101 2.303361745515736 1103 1 0.5308039437942398 1103 2 0.4578067688517784 1103 3 0.4318119190619214 1103 4 0.40445942642543997 1103 5 0.24607250427598615 1103 6 0.27705151426035 1103 7 0.38558644385832963 1103 8 0.3864934399426474 1103 9 0.5088860136118001 1103 10-11 0.5292771166806745 1103 12-13 0.46291416783468975 1103 14-15 0.5231208440885595 1103 16-17 0.5945100105142984 1103 18-19 0.6758629747365745 1103 20-21 0.6961781889556633 1103 22-23 0.7716989796084164 1103 24-25 0.732591259899678 1103 26-27 0.7427823831934717 1103 28-29 0.772676155292146 1103 30-31 0.8147136947782982 1103 32-33 0.9298532761786689 1103 34-35 0.7087882562490151 1103 36-37 0.8004487463178336 1103 38-39 0.9546700839457003 1103 40-41 1.2129412627192124 1103 42-43 1.2390454420234178 1103 44-45 1.3188919951685492 1103 46-47 1.6128608894493226 1103 48-49 1.3146456700905773 1103 50-51 1.4335419388777737 1103 52-53 1.505180113233937 1103 54-55 1.4950666007432503 1103 56-57 1.599271984688567 1103 58-59 1.764376669864813 1103 60-61 2.061517489298968 1103 62-63 2.2201483056315503 1103 64-65 2.215812137267129 1103 66-67 2.433659673372059 1103 68-69 2.5567849724673373 1103 70-71 2.3570960715609566 1103 72-73 2.6637273650326634 1103 74-75 2.57713948350456 1103 76-77 2.7402548966026004 1103 78-79 3.2267871227795943 1103 80-81 3.1096046246566935 1103 82-83 3.174784318174307 1103 84-85 3.4670339166884325 1103 86-87 3.5427613114102385 1103 88-89 3.737269329565546 1103 90-91 3.718335520813012 1103 92-93 3.6151424361687017 1103 94-95 3.588597004370973 1103 96-97 3.490607734130803 1103 98-99 3.0620515319260946 1103 100-101 2.07107523715307 1104 1 0.49344293926869653 1104 2 0.46906378376112556 1104 3 0.4615406074752002 1104 4 0.31090493198841784 1104 5 0.36194917451129527 1104 6 0.38422132796456765 1104 7 0.4891165578338246 1104 8 0.521501824673912 1104 9 0.5711256684956254 1104 10-11 0.5875825798620724 1104 12-13 0.6214815157015536 1104 14-15 0.6712167521502224 1104 16-17 0.7339973576232239 1104 18-19 0.7084339449336667 1104 20-21 0.7701019905391249 1104 22-23 0.720368554876778 1104 24-25 0.7336748798297563 1104 26-27 0.7232918306223084 1104 28-29 0.8161843836979017 1104 30-31 0.7642351650590768 1104 32-33 0.8146469107307084 1104 34-35 0.9106321076039947 1104 36-37 1.1312390269773687 1104 38-39 0.9010524119008068 1104 40-41 0.8492673608663566 1104 42-43 0.9911814957362282 1104 44-45 0.9567063436386221 1104 46-47 1.349371893816965 1104 48-49 1.3163640662857006 1104 50-51 1.32726389108322 1104 52-53 1.4900607531894785 1104 54-55 1.6557802661182563 1104 56-57 1.7489990466850465 1104 58-59 1.9052575245705263 1104 60-61 1.985689753681708 1104 62-63 2.032524547945549 1104 64-65 1.9898882377828464 1104 66-67 2.3232141299119924 1104 68-69 2.6336635628218943 1104 70-71 2.6645330597528556 1104 72-73 2.9962782813527262 1104 74-75 2.963304519017292 1104 76-77 2.9856200034881866 1104 78-79 3.290241458364644 1104 80-81 3.36055639429291 1104 82-83 3.5990023748746722 1104 84-85 3.434133320756686 1104 86-87 3.35995206325693 1104 88-89 3.448599691135371 1104 90-91 3.2979430964413954 1104 92-93 3.2226837203116094 1104 94-95 3.2478172875253986 1104 96-97 3.02263274936859 1104 98-99 2.651644596126923 1104 100-101 2.014842271105704 1105 1 0.48840395575736295 1105 2 0.48195886964315093 1105 3 0.3865516102792874 1105 4 0.23904759850670843 1105 5 0.4085920410349999 1105 6 0.3286388710960608 1105 7 0.27282498903948493 1105 8 0.3712280829285248 1105 9 0.4505198977128586 1105 10-11 0.5359658944981618 1105 12-13 0.5983524435361929 1105 14-15 0.6300034812145654 1105 16-17 0.7610459594400183 1105 18-19 0.6714171535017215 1105 20-21 0.6260111229166441 1105 22-23 0.6320537583860499 1105 24-25 0.7948972136673689 1105 26-27 0.6838888843771471 1105 28-29 0.6689407991597776 1105 30-31 0.7551267731527318 1105 32-33 0.8455006280744399 1105 34-35 0.8884701042214473 1105 36-37 0.950782012100774 1105 38-39 1.164099757543248 1105 40-41 1.3517694336041686 1105 42-43 1.1852803585625935 1105 44-45 1.2265552518296161 1105 46-47 1.3768032127599312 1105 48-49 1.42402625363313 1105 50-51 1.2439166475989367 1105 52-53 1.388765780794234 1105 54-55 1.7078598712805046 1105 56-57 2.0353698306561405 1105 58-59 1.7618556480284973 1105 60-61 1.885381650103966 1105 62-63 2.1284324071056453 1105 64-65 2.179367927959923 1105 66-67 2.422934372966189 1105 68-69 2.750492163696304 1105 70-71 2.6025579486470924 1105 72-73 2.7354642041797135 1105 74-75 2.9113783014655397 1105 76-77 3.083352097489726 1105 78-79 3.4174322444811622 1105 80-81 3.421478720009997 1105 82-83 3.807321923373731 1105 84-85 3.696398690345635 1105 86-87 3.7916233967165844 1105 88-89 3.657011472707403 1105 90-91 3.4516349758205873 1105 92-93 3.7943251331618857 1105 94-95 3.6526702259006765 1105 96-97 3.6565633169127736 1105 98-99 3.1887306876802786 1105 100-101 2.3876398305383892 1106 1 0.3480187710299809 1106 2 0.29965951389378986 1106 3 0.17343852300989226 1106 4 0.1899375675152939 1106 5 0.28942685549021974 1106 6 -0.059898192504626024 1106 7 0.36285510324618997 1106 8 0.13674621973533618 1106 9 0.3124844883500444 1106 10-11 0.4435323007598413 1106 12-13 0.42482331469503976 1106 14-15 0.6255260987681268 1106 16-17 0.18198594933216583 1106 18-19 0.5604392990567106 1106 20-21 0.5152044738672714 1106 22-23 0.4718719218082015 1106 24-25 0.4998105094734129 1106 26-27 0.5604840601231018 1106 28-29 0.5411455657971018 1106 30-31 0.47530762785392966 1106 32-33 0.6802408195254301 1106 34-35 0.6187365018107158 1106 36-37 0.7444644333188819 1106 38-39 0.8627608167140224 1106 40-41 0.9491303535179227 1106 42-43 0.5885065709808828 1106 44-45 1.1038060919073232 1106 46-47 1.1652816877310528 1106 48-49 1.087531269178676 1106 50-51 0.7853746447101315 1106 52-53 1.095786622496778 1106 54-55 0.8520276485809148 1106 56-57 1.3673577769988192 1106 58-59 1.5000464630459298 1106 60-61 1.7269926106385078 1106 62-63 1.8621312610512462 1106 64-65 1.855013075744818 1106 66-67 2.0455348807509246 1106 68-69 2.205399603131461 1106 70-71 2.231493429881283 1106 72-73 2.3617083714835196 1106 74-75 2.7193981154372935 1106 76-77 2.886395242246259 1106 78-79 3.206021367480897 1106 80-81 3.0892803181263915 1106 82-83 3.1661021434935286 1106 84-85 3.1020410918664005 1106 86-87 2.845836694217237 1106 88-89 3.0960644701226236 1106 90-91 3.049752987425668 1106 92-93 3.4777570113573457 1106 94-95 3.5836452942083863 1106 96-97 3.1344552676175823 1106 98-99 2.9492392599120105 1106 100-101 1.800032758634118 1107 1 0.1273487611449866 1107 2 0.1346955579664062 1107 3 -0.008248194930171593 1107 4 0.13631435465033093 1107 5 0.07263465740453512 1107 6 0.015808825842889007 1107 7 0.22044557507395268 1107 8 0.17489917197392657 1107 9 0.33722639350229144 1107 10-11 0.09936890900054607 1107 12-13 0.21418404473819663 1107 14-15 0.23375391177292926 1107 16-17 0.16201777419896501 1107 18-19 0.17670120324457628 1107 20-21 0.3409947913926388 1107 22-23 0.31606834392166405 1107 24-25 0.3679016202094729 1107 26-27 0.31113972470829054 1107 28-29 0.36883247661514673 1107 30-31 0.42973321311804824 1107 32-33 0.4207001102166572 1107 34-35 0.557034474180945 1107 36-37 0.465244022006587 1107 38-39 0.719079981552369 1107 40-41 0.5155484888700457 1107 42-43 0.678648215747863 1107 44-45 0.5568811696612599 1107 46-47 0.7032088521836357 1107 48-49 0.608533873031412 1107 50-51 0.9884068071133925 1107 52-53 1.0862339788629534 1107 54-55 1.117719598336194 1107 56-57 1.1084060688232036 1107 58-59 1.1557693938268478 1107 60-61 1.3992431350255146 1107 62-63 1.4000992915259616 1107 64-65 1.57638359434549 1107 66-67 1.6759589712106973 1107 68-69 1.8084972234993764 1107 70-71 1.8705935335532011 1107 72-73 1.83464204556795 1107 74-75 1.9835125644090112 1107 76-77 1.9731817745412634 1107 78-79 2.3751706550378593 1107 80-81 2.3418792283658014 1107 82-83 2.4636299299776034 1107 84-85 2.4990300254922637 1107 86-87 2.503794598597974 1107 88-89 2.5951532422131827 1107 90-91 2.5630865216735668 1107 92-93 2.4598230961754357 1107 94-95 2.3744411569757045 1107 96-97 2.3390420265442167 1107 98-99 2.461766925847348 1107 100-101 1.449824691512581 1108 1 0.17119110097150525 1108 2 0.1968938185980953 1108 3 0.13366852967744336 1108 4 0.13596123780718017 1108 5 0.01935436378639821 1108 6 0.19285851068212168 1108 7 0.014620856894936196 1108 8 0.08072275033099174 1108 9 0.20126979792252087 1108 10-11 0.1627055098235033 1108 12-13 0.21293012660020594 1108 14-15 0.27856760787359036 1108 16-17 0.3223551614990683 1108 18-19 0.3005311190117226 1108 20-21 0.2992634018356952 1108 22-23 0.4310799131145515 1108 24-25 0.3365763492168057 1108 26-27 0.29522461864785754 1108 28-29 0.316923502796854 1108 30-31 0.3428407097270778 1108 32-33 0.5523510283432458 1108 34-35 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-0.5843878623679046 2315 30-31 -0.7435149588900458 2315 32-33 -0.6074502692982335 2315 34-35 -0.5614519311258874 2315 36-37 -0.7234691064834067 2315 38-39 -0.587601104167554 2315 40-41 -0.5197890155513463 2315 42-43 -0.6581915762002666 2315 44-45 -0.7926660797980176 2315 46-47 -0.7351202965316901 2315 48-49 -0.8127986910280285 2315 50-51 -0.7578510426694578 2315 52-53 -0.6091150646667352 2315 54-55 -0.5443319855368856 2315 56-57 -0.3596170325875754 2315 58-59 -0.33252461569436775 2315 60-61 -0.545121025315904 2315 62-63 -0.37280670823049533 2315 64-65 -0.21401522970920084 2315 66-67 -0.12236669837650282 2315 68-69 0.034603557252136596 2315 70-71 -0.256039656169456 2315 72-73 -0.3145570011509413 2315 74-75 -0.19345502751244936 2315 76-77 -0.14418122029222857 2315 78-79 -0.20174057500789289 2315 80-81 0.044096476723364475 2315 82-83 -0.20470020449069892 2315 84-85 -0.30452570144256796 2315 86-87 -0.18503461680064603 2315 88-89 -0.31127433103696234 2315 90-91 -0.16273823464250192 2315 92-93 -0.22082906639262312 2315 94-95 -0.2687594089624721 2315 96-97 -0.27099740357459545 2315 98-99 -0.5531600414546478 2315 100-101 -0.2653200692103823 2316 1 -1.0045548726729763 2316 2 -0.8682843972533298 2316 3 -0.7536572726812345 2316 4 -0.7434628074152343 2316 5 -0.638227096823897 2316 6 -0.5206906336037918 2316 7 -0.7384986031963976 2316 8 -0.6606193752078084 2316 9 -0.7954399226416413 2316 10-11 -0.8333734390685237 2316 12-13 -0.7713428322814977 2316 14-15 -1.0371789119239878 2316 16-17 -1.0129025606487119 2316 18-19 -1.0319426873214468 2316 20-21 -1.1420155293356586 2316 22-23 -1.1764246407006311 2316 24-25 -1.0434631144467872 2316 26-27 -1.1138362605461154 2316 28-29 -1.183377057209313 2316 30-31 -1.0835861386905918 2316 32-33 -1.380572346376617 2316 34-35 -0.9823546119590318 2316 36-37 -1.2485327028901736 2316 38-39 -1.3287716882552232 2316 40-41 -1.3610198542011247 2316 42-43 -1.054969119767378 2316 44-45 -1.2223728667655323 2316 46-47 -0.7898926832240818 2316 48-49 -0.5757342532276581 2316 50-51 -0.6177051080994929 2316 52-53 -0.575151787157214 2316 54-55 -0.551339425359533 2316 56-57 -0.619406610253538 2316 58-59 -0.26331396953722574 2316 60-61 -0.6455910657531199 2316 62-63 -0.4572073351259718 2316 64-65 -0.36663044822529756 2316 66-67 -0.4334930317904089 2316 68-69 -0.48403451554629484 2316 70-71 -0.32771474134326795 2316 72-73 -0.12713085114796385 2316 74-75 -0.12116809559772079 2316 76-77 0.14078159967374404 2316 78-79 0.04703033566832815 2316 80-81 -0.0494616392370375 2316 82-83 0.07182548621582541 2316 84-85 0.4196015941602589 2316 86-87 0.39196351117012185 2316 88-89 0.06275052704046757 2316 90-91 -0.09106380531687996 2316 92-93 0.08664818730150081 2316 94-95 0.23197836063138055 2316 96-97 0.11616445153136823 2316 98-99 0.15263671452940208 2316 100-101 0.4907433367619145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9381.0 3 3578.0 4 4024.0 5 4694.0 6 5788.0 7 7237.0 8 9250.0 9 12149.0 10 15612.0 11 19209.0 12 23528.0 13 27626.0 14 31396.0 15 35781.0 16 41228.0 17 46855.0 18 47702.0 19 48761.0 20 53286.0 21 58534.0 22 65063.0 23 72073.0 24 79847.0 25 88273.0 26 96607.0 27 105584.0 28 115874.0 29 126408.0 30 139641.0 31 154855.0 32 171070.0 33 190520.0 34 210696.0 35 224353.0 36 215234.0 37 146385.0 38 34109.0 39 476.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.69128332310954 18.29866758802835 14.131055403215298 23.878993685646815 2 15.583666258427678 19.518188031619434 40.15962898151545 24.738516728437432 3 19.527401382379225 23.298962303722213 29.206134991702076 27.967501322196487 4 12.983243027533845 15.97971274803697 36.33652704833178 34.7005171760974 5 14.10281341885977 36.93269445120436 33.97311543332327 14.991376696612594 6 33.35017584309834 35.231186247756355 17.07243130444934 14.34620660469596 7 29.500124927733413 31.98244810939657 20.110301157906832 18.407125804963187 8 26.705408853849406 34.96021582014793 19.40550245081053 18.928872875192138 9 26.539430922480424 16.76156155279888 19.779474254366928 36.91953327035377 10-11 25.335579986500385 26.205121574053013 27.15573016015155 21.303568279295053 12-13 26.105934864639917 23.35139204273984 27.56103841347904 22.981634679141205 14-15 22.722732853696563 24.994251178895833 25.578433523295168 26.704582444112436 16-17 22.649845469565918 28.268046527072322 26.418276476454462 22.663831526907295 18-19 22.630144080762545 26.818260563521545 28.51779719278666 22.033798162929248 20-21 23.001768084568972 26.070065024296348 28.700453282536998 22.22771360859769 22-23 22.757096832198933 25.975104324682558 28.57976547964552 22.688033363472993 24-25 22.6346816330759 26.18938013327361 28.61233269668217 22.563605536968325 26-27 22.282043332360665 26.553855412897576 28.38493945141523 22.779161803326524 28-29 22.319242788531678 26.818204033636984 28.469598182762507 22.39295499506883 30-31 22.526903369632457 26.36280375713435 28.367918169403687 22.742374703829498 32-33 22.185484628353272 26.386530102751188 28.39156873132512 23.036416537570425 34-35 22.932517510127123 26.5682156839896 28.041023127209225 22.45824367867405 36-37 22.923913515256363 26.14529842517933 28.153972576958385 22.776815482605922 38-39 22.42961770641352 26.5536397643093 28.147848582070555 22.868893947206626 40-41 22.871936489796248 26.32157793122159 28.268374770881156 22.53811080810101 42-43 22.657129599156907 26.227312466895736 28.11501003270086 23.0005479012465 44-45 22.64643810777524 26.580395667548824 28.115259756538926 22.657906468137014 46-47 22.88580927604867 26.793367160409232 27.50058486548641 22.820238698055697 48-49 22.741103904297272 26.72402538438704 27.578917772061274 22.955952939254413 50-51 22.887441300087737 26.950926545159643 27.429149871387114 22.732482283365506 52-53 23.109327504827917 26.710883669099587 27.48933045116949 22.690458374903006 54-55 22.61633820730525 26.841541125380115 27.677243256833506 22.864877410481128 56-57 22.64966606968643 27.077699989443605 27.508980684349755 22.763653256520215 58-59 22.70950232975845 26.56074126390718 27.58582404243628 23.143932363898095 60-61 22.519873157467824 26.777389824556863 27.872412150352122 22.830324867623194 62-63 22.570050747260844 26.776003709274153 27.87739047531183 22.776555068153172 64-65 22.35095733637003 26.693802956696068 27.731669421499657 23.223570285434246 66-67 22.29318964344796 26.876276401758492 27.635052922930402 23.195481031863142 68-69 22.65715345747178 26.494688701286506 27.8145963403862 23.033561500855516 70-71 22.492899211282786 26.640401618932426 27.533957796755352 23.332741373029435 72-73 22.549331882519844 26.590289893466217 27.463075271220745 23.397302952793193 74-75 22.501398443807354 26.89954979955031 27.281687788577173 23.31736396806516 76-77 22.584597254208166 26.61451640897217 27.39483774800148 23.406048588818184 78-79 22.665517795260833 26.686318542343272 27.364428585675732 23.283735076720166 80-81 22.69599209431396 26.781113141015123 27.132402008511008 23.390492756159908 82-83 22.70280736992038 26.74874429398581 27.145366523209503 23.403081812884306 84-85 22.65345551930832 26.58616997361648 27.146730921082256 23.613643585992943 86-87 22.78896744342068 26.525772895161907 27.057966681372854 23.627292980044558 88-89 22.683295322684515 26.644036533983122 26.905577026446515 23.767091116885844 90-91 22.613459998625054 26.57474704354551 27.003041112182334 23.808751845647098 92-93 22.786712067818762 26.163191810872938 27.086536161448795 23.96355995985951 94-95 22.56214839467759 25.999045974823364 27.160287170330694 24.278518460168357 96-97 22.788585494826094 25.740110315904634 27.063135342116055 24.40816884715321 98-99 22.62990875551419 25.85853594945225 26.69936359500904 24.81219170002452 100-101 23.46070834285564 24.920140392277695 26.991707743406607 24.62744352146006 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 309.0 1 243.5 2 239.0 3 326.0 4 304.0 5 282.0 6 481.5 7 999.0 8 1617.0 9 1940.0 10 1782.0 11 1563.5 12 1582.0 13 1736.5 14 1908.5 15 2071.0 16 2295.0 17 2569.0 18 2787.5 19 3065.5 20 3585.0 21 4285.5 22 5270.0 23 6492.5 24 8045.5 25 10084.0 26 12820.5 27 16207.5 28 19476.0 29 23140.5 30 27618.5 31 33454.0 32 40725.0 33 45191.0 34 47602.5 35 51902.5 36 58037.0 37 63975.5 38 68777.0 39 73128.5 40 77677.5 41 83529.5 42 87979.0 43 92373.0 44 96807.5 45 101575.5 46 104891.5 47 105331.5 48 106192.0 49 105776.0 50 103393.0 51 100752.0 52 98972.5 53 97558.5 54 95803.0 55 90410.5 56 83006.0 57 76135.0 58 69784.0 59 61792.0 60 51582.5 61 42872.0 62 36014.0 63 28723.5 64 22031.5 65 17350.0 66 13457.0 67 10646.0 68 8230.0 69 6117.0 70 4717.0 71 3513.5 72 2634.0 73 2032.5 74 1539.0 75 1202.0 76 879.0 77 588.5 78 398.0 79 245.5 80 141.0 81 90.0 82 54.5 83 34.0 84 25.0 85 21.0 86 11.5 87 5.0 88 5.5 89 4.5 90 4.5 91 3.0 92 2.5 93 3.0 94 2.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3827997872159674 2 0.030225833279553957 3 0.03780963704571466 4 0.04597681033234926 5 0.06774378556503168 6 0.05859217621259735 7 0.04017957572263988 8 0.041747381308913485 9 0.03853884894630703 10-11 0.041109320895895154 12-13 0.03241346898133108 14-15 0.029915918221802194 16-17 0.024446828967359384 18-19 0.029970609114346625 20-21 0.03717157663269633 22-23 0.03667935859979647 24-25 0.04590388914229002 26-27 0.0396326667971956 28-29 0.034382341112930495 30-31 0.0372991887153 32-33 0.03170248737825351 34-35 0.033416135344645596 36-37 0.03565846193896715 38-39 0.016370807168298824 40-41 0.01412848057397727 42-43 0.01474831068948079 44-45 0.013107583913147945 46-47 0.022003969100374925 48-49 0.019287654770668326 50-51 0.012724747665336949 52-53 0.011047560293974485 54-55 0.009570906195274925 56-57 0.009971972740600733 58-59 0.004666956163791202 60-61 0.008440627749356744 62-63 0.01354511105350337 64-65 0.00853177923693079 66-67 0.004156507833376539 68-69 0.006726979782964662 70-71 0.014073789681432844 72-73 0.011576238921903957 74-75 0.012870590045455425 76-77 0.005943076989827859 78-79 0.010409499880956158 80-81 0.013016432425573899 82-83 0.014766540986995598 84-85 0.009315682030067593 86-87 0.002807465817280645 88-89 0.017245861449009674 90-91 0.027801203710084307 92-93 0.011630929814448385 94-95 0.022642029513393253 96-97 0.020490854406645744 98-99 0.012013766062259382 100-101 0.12779438557881379 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2742687.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.864570497959654 #Duplication Level Percentage of deduplicated Percentage of total 1 78.76798415286326 45.578755719955254 2 12.274471520605003 14.205140452584924 3 3.8652581905226566 6.709845151749427 4 1.6783206023886086 3.88461203260375 5 0.902641893977151 2.611549275422634 6 0.5488126419260098 1.9054084685339447 7 0.35513594080264854 1.4384852078053858 8 0.25070196038744225 1.1605409008652674 9 0.1974776874854387 1.028426541234769 >10 1.0339280231613897 10.93330376583818 >50 0.07986822482530434 3.204609016831844 >100 0.040833034288596914 4.185160063001135 >500 0.002726992374510365 1.1181515137257 >1k 0.0017122975374510878 1.5941457140492155 >5k 1.268368546260065E-4 0.4418661757985913 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6739 0.24570794990460085 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5302 0.1933140748470387 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3515 0.12815899152910995 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.8230297514809382E-5 0.0 16-17 0.0 0.0 0.0 7.292119005923753E-5 0.0 18-19 0.0 0.0 0.0 9.115148757404691E-5 0.0 20-21 0.0 0.0 0.0 1.8230297514809383E-4 0.0 22-23 0.0 0.0 0.0 4.5575743787023453E-4 0.0 24-25 0.0 0.0 0.0 6.745210080479472E-4 0.0 26-27 0.0 0.0 0.0 0.0010573572558589441 0.0 28-29 0.0 0.0 0.0 0.0030809202800027857 0.0 30-31 0.0 0.0 0.0 0.010208966608293254 0.0 32-33 0.0 0.0 0.0 0.025449495330673897 0.0 34-35 0.0 0.0 0.0 0.049312954777559384 1.8230297514809382E-5 36-37 0.0 0.0 0.0 0.09060457864860263 3.6460595029618764E-5 38-39 0.0 0.0 0.0 0.15377255953741714 3.6460595029618764E-5 40-41 0.0 0.0 0.0 0.22350344753156304 3.6460595029618764E-5 42-43 0.0 0.0 0.0 0.2940000080213309 3.6460595029618764E-5 44-45 0.0 0.0 0.0 0.3878860402225992 3.6460595029618764E-5 46-47 0.0 0.0 0.0 0.49573648032021156 3.6460595029618764E-5 48-49 0.0 0.0 0.0 0.6099492942504923 3.6460595029618764E-5 50-51 0.0 0.0 0.0 0.7378348313168801 3.6460595029618764E-5 52-53 0.0 0.0 0.0 0.8630952055411354 3.6460595029618764E-5 54-55 0.0 0.0 0.0 0.9995307521419688 3.6460595029618764E-5 56-57 0.0 0.0 0.0 1.1619809332964353 3.6460595029618764E-5 58-59 0.0 0.0 0.0 1.3347494628442838 3.6460595029618764E-5 60-61 0.0 0.0 0.0 1.5304699369632773 3.6460595029618764E-5 62-63 0.0 0.0 0.0 1.7211770792656982 3.6460595029618764E-5 64-65 0.0 0.0 0.0 1.916788171599603 3.6460595029618764E-5 66-67 0.0 0.0 0.0 2.14561486600549 3.6460595029618764E-5 68-69 0.0 0.0 0.0 2.3985967046185 3.6460595029618764E-5 70-71 0.0 0.0 0.0 2.668696792597916 3.6460595029618764E-5 72-73 0.0 0.0 0.0 2.9472921992192327 3.6460595029618764E-5 74-75 0.0 0.0 0.0 3.2223691584201912 3.6460595029618764E-5 76-77 0.0 0.0 0.0 3.515858718111108 3.6460595029618764E-5 78-79 0.0 0.0 0.0 3.8504575986979193 3.6460595029618764E-5 80-81 0.0 0.0 0.0 4.208281878318598 3.6460595029618764E-5 82-83 0.0 0.0 0.0 4.570955417078215 3.6460595029618764E-5 84-85 0.0 0.0 0.0 4.933556034647774 3.6460595029618764E-5 86-87 0.0 0.0 0.0 5.308370951552256 3.6460595029618764E-5 88-89 0.0 0.0 0.0 5.702127147574624 3.6460595029618764E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4590 0.0 44.244946 1 GTATCAA 7650 0.0 40.007404 1 ATCAACG 9640 0.0 31.010933 3 TATCAAC 9880 0.0 30.546255 2 TCAACGC 9845 0.0 30.316925 4 CAACGCA 9960 0.0 29.96688 5 AACGCAG 10370 0.0 28.730999 6 GTACATG 8660 0.0 25.267456 1 GTGGTAT 2505 0.0 25.12077 1 ACGCAGA 11895 0.0 24.841908 7 GGACCGA 680 0.0 24.45801 6 CGCAGAG 12080 0.0 24.304155 8 TAAGGTG 1330 0.0 24.299564 5 TACATGG 8825 0.0 24.073212 2 GTAAGGT 1285 0.0 22.561497 4 ACATGGG 9060 0.0 22.452091 3 GCAGAGT 12950 0.0 22.04571 9 TGGTATC 2610 0.0 22.033632 2 GTATAGG 745 0.0 21.11662 1 AGGACCG 910 0.0 20.891027 5 >>END_MODULE