FastQCFastQC Report
Thu 26 May 2016
SRR938580_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938580_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742687
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67890.24753097965608178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55620.20279382955473957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39230.14303491430119442No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44900.052.483131
GTATCAA73450.039.392171
ATCAACG93350.030.427093
TATCAAC95300.030.0060482
TCAACGC95800.029.6985234
CAACGCA96850.029.278465
AACGCAG101650.027.989366
ACGCAGA116500.024.3808467
CGCAGAG118000.023.9501638
GTACATG88250.023.2569851
TACATGG87450.022.97662
GTGGTAT21150.021.1155241
ACATGGG90600.021.0227583
GAGTACT78750.021.01966312-13
GCAGAGT131650.020.7814169
GTACTTT82200.019.93521114-15
CAGAGTA127200.019.26797710-11
TGGTATC21600.019.1324562
AGTACTT83950.018.67096512-13
AGAGTAC122550.018.6037910-11
Produced by FastQC (version 0.11.5)