##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938580_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2742687 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79914915555439 31.0 31.0 34.0 30.0 34.0 2 32.05165080813086 31.0 31.0 34.0 30.0 34.0 3 32.11224211876893 33.0 31.0 34.0 30.0 34.0 4 35.7662307073319 37.0 35.0 37.0 35.0 37.0 5 35.66108454956763 37.0 35.0 37.0 33.0 37.0 6 35.692367740103045 37.0 35.0 37.0 33.0 37.0 7 35.61172638365223 37.0 35.0 37.0 33.0 37.0 8 35.580861760747766 37.0 35.0 37.0 33.0 37.0 9 37.12230743063281 39.0 37.0 39.0 33.0 39.0 10-11 37.04901890007865 39.0 37.0 39.0 33.0 39.0 12-13 36.92564864310073 39.0 37.0 39.0 33.0 39.0 14-15 37.941483844128044 40.0 37.0 41.0 33.0 41.0 16-17 38.03204649309236 40.0 37.0 41.0 33.0 41.0 18-19 38.163090976112116 40.0 37.5 41.0 33.0 41.0 20-21 38.10833500140556 40.0 37.0 41.0 33.0 41.0 22-23 37.98221853241 40.0 37.0 41.0 33.0 41.0 24-25 37.85753405328424 40.0 37.0 41.0 32.0 41.0 26-27 37.64190828191478 40.0 37.0 41.0 32.0 41.0 28-29 37.49831934887211 39.5 36.5 41.0 32.0 41.0 30-31 37.23782061168482 39.0 36.0 41.0 31.0 41.0 32-33 37.13755051159684 39.0 36.0 41.0 31.0 41.0 34-35 37.00843041878275 39.0 36.0 41.0 31.0 41.0 36-37 36.881115672331546 39.0 36.0 41.0 30.0 41.0 38-39 36.66925992648815 39.0 35.0 40.0 30.0 41.0 40-41 36.443034330931674 39.0 35.0 40.0 30.0 41.0 42-43 36.18112912629111 38.5 35.0 40.0 30.0 41.0 44-45 35.89426792047361 38.0 35.0 40.0 29.0 41.0 46-47 35.48433616376933 38.0 34.5 40.0 27.5 41.0 48-49 35.078641857419385 38.0 34.0 40.0 26.5 41.0 50-51 34.67117866530158 38.0 33.0 40.0 25.5 41.0 52-53 34.05686613164389 37.5 33.0 40.0 24.0 41.0 54-55 33.61179037199651 37.0 32.0 40.0 23.0 41.0 56-57 33.207983812954225 37.0 32.0 39.0 22.0 40.5 58-59 32.65933115955266 36.0 31.0 39.0 19.5 40.0 60-61 32.14674806129901 35.5 31.0 39.0 18.0 40.0 62-63 31.604067835666264 35.0 30.0 38.5 14.0 40.0 64-65 30.932291216606195 34.5 29.0 38.0 9.0 40.0 66-67 30.254791013338377 34.0 28.0 38.0 7.5 40.0 68-69 29.50462083351108 34.0 27.5 37.0 4.5 39.0 70-71 28.70547842316677 33.5 26.0 36.0 2.0 39.0 72-73 28.101773735027002 33.0 26.0 36.0 2.0 38.5 74-75 27.364312624809173 32.0 25.0 35.0 2.0 38.0 76-77 26.236692520874605 30.5 23.0 34.0 2.0 36.0 78-79 26.71713815685129 32.0 25.0 35.0 2.0 36.0 80-81 26.319108596788478 32.0 23.5 35.0 2.0 36.0 82-83 25.61244082901184 31.0 21.0 34.0 2.0 36.0 84-85 24.80412055768668 31.0 19.0 34.0 2.0 35.0 86-87 23.860266227972787 30.5 11.5 34.0 2.0 35.0 88-89 22.864548707161994 29.5 4.5 34.0 2.0 35.0 90-91 21.978861423122652 29.0 2.0 34.0 2.0 35.0 92-93 20.864218738776973 28.0 2.0 33.0 2.0 35.0 94-95 19.652400000437527 26.5 2.0 33.0 2.0 34.0 96-97 18.251582116369825 24.5 2.0 32.0 2.0 34.0 98-99 16.473371186723092 11.0 2.0 32.0 2.0 34.0 100-101 12.33327189723071 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2899526441848188 1101 2 0.2779528788802921 1101 3 0.22294678117534517 1101 4 0.18862097931872057 1101 5 0.1528291886049118 1101 6 0.16768204156580424 1101 7 0.31525722022124114 1101 8 0.29551421390349475 1101 9 0.39455195048731895 1101 10-11 0.3072815424358808 1101 12-13 0.3253856700449873 1101 14-15 0.4119575060002063 1101 16-17 0.31121561340711423 1101 18-19 0.4594364212324251 1101 20-21 0.40357427078582475 1101 22-23 0.41564286687655994 1101 24-25 0.3054325810705478 1101 26-27 0.37028232206930767 1101 28-29 0.45717492729286846 1101 30-31 0.526116038104 1101 32-33 0.4299082945026882 1101 34-35 0.2286069204131067 1101 36-37 0.3001358040586197 1101 38-39 0.48280454171221976 1101 40-41 0.6073847840743909 1101 42-43 0.6470791519074481 1101 44-45 0.5323853172809478 1101 46-47 0.7390830320075352 1101 48-49 0.8649568003842276 1101 50-51 0.9298555171075549 1101 52-53 1.1871341882653113 1101 54-55 1.1748920959979685 1101 56-57 1.110910613345773 1101 58-59 1.203623104764901 1101 60-61 1.246202049015487 1101 62-63 1.3655182826546088 1101 64-65 1.2312128985796171 1101 66-67 1.548450806253424 1101 68-69 1.8277987994258105 1101 70-71 1.9405938556800422 1101 72-73 1.980858912232474 1101 74-75 2.0399641093907626 1101 76-77 2.0741053481666896 1101 78-79 2.124054810582642 1101 80-81 2.3284827353464514 1101 82-83 2.2673422570881137 1101 84-85 2.452416724197189 1101 86-87 2.506232146048493 1101 88-89 2.3156706565512124 1101 90-91 2.6140704785883315 1101 92-93 2.9158116640056626 1101 94-95 2.7645416541311825 1101 96-97 2.6288034759565697 1101 98-99 2.7414291125050774 1101 100-101 2.362516392865558 1102 1 0.25411472293023607 1102 2 0.23180256189664306 1102 3 0.21935986092175597 1102 4 0.13457735235842705 1102 5 0.15562364973270348 1102 6 0.2143641403312344 1102 7 0.26213117751186843 1102 8 0.22767554189815797 1102 9 0.3283294780148509 1102 10-11 0.32203066656000345 1102 12-13 0.265356891266201 1102 14-15 0.3696756408016739 1102 16-17 0.38212506452489237 1102 18-19 0.30431776090542684 1102 20-21 0.35163170321825277 1102 22-23 0.42193040441409124 1102 24-25 0.30313862044325646 1102 26-27 0.37304028836893366 1102 28-29 0.27351209779670427 1102 30-31 0.25925542749338604 1102 32-33 0.24731945566384894 1102 34-35 0.31959374056659584 1102 36-37 0.35740661653776584 1102 38-39 0.317785355859705 1102 40-41 0.5331751577814288 1102 42-43 0.3744679990879547 1102 44-45 0.4360441844814815 1102 46-47 0.43011260324543343 1102 48-49 0.4755100619791577 1102 50-51 0.5426193225796894 1102 52-53 0.5683748038809213 1102 54-55 0.4632794416769812 1102 56-57 0.8086708736060331 1102 58-59 0.7751998898833534 1102 60-61 0.8594976362694027 1102 62-63 0.8099418729115406 1102 64-65 1.0526332772916653 1102 66-67 1.2831594732537575 1102 68-69 1.4679597937534723 1102 70-71 1.5923184969880175 1102 72-73 1.6572279896432178 1102 74-75 1.398040936217587 1102 76-77 1.5332415677195783 1102 78-79 1.7020234041762379 1102 80-81 1.918213090701805 1102 82-83 2.009271686517547 1102 84-85 2.1461260585315216 1102 86-87 1.9249842314672456 1102 88-89 1.9393151343623565 1102 90-91 2.2598360358122243 1102 92-93 2.3531604711461362 1102 94-95 2.150537024501549 1102 96-97 2.2994793601741215 1102 98-99 2.250260860920161 1102 100-101 1.792477604076769 1103 1 0.25156913098066624 1103 2 0.21065973032334284 1103 3 0.21486281420616393 1103 4 0.10962856250897346 1103 5 0.12395787800070934 1103 6 0.18281520729223644 1103 7 0.2566024699964018 1103 8 0.22532882431965362 1103 9 0.21998272066146995 1103 10-11 0.3478022991197136 1103 12-13 0.3878013555980857 1103 14-15 0.5162646967450399 1103 16-17 0.29845985958485954 1103 18-19 0.31326710760311727 1103 20-21 0.39247925606911593 1103 22-23 0.47765997504432534 1103 24-25 0.37425360960460097 1103 26-27 0.40559602291143193 1103 28-29 0.44269328229245986 1103 30-31 0.45465571188373843 1103 32-33 0.42499506173349033 1103 34-35 0.49656334261897683 1103 36-37 0.4657894198709016 1103 38-39 0.4867800956325894 1103 40-41 0.6255175001237703 1103 42-43 0.5917394924356074 1103 44-45 0.813605934973701 1103 46-47 0.8710108690609246 1103 48-49 0.8909039326710371 1103 50-51 0.8830673865376895 1103 52-53 0.8439935517678876 1103 54-55 0.9579446366732967 1103 56-57 1.204162391614112 1103 58-59 1.1831819613588053 1103 60-61 1.2104897707880298 1103 62-63 1.1742113576280424 1103 64-65 1.5495395378568695 1103 66-67 1.3846674381723183 1103 68-69 1.4012647874889268 1103 70-71 1.4834434795865121 1103 72-73 1.4923039596529648 1103 74-75 1.406632309047371 1103 76-77 1.5992591581576932 1103 78-79 1.8354656212277263 1103 80-81 1.7934194228617777 1103 82-83 2.0941910429600803 1103 84-85 1.949086303544746 1103 86-87 2.014086164526134 1103 88-89 2.160383885232431 1103 90-91 2.3049034542240108 1103 92-93 2.3511207658779547 1103 94-95 2.0367125211902923 1103 96-97 1.9753601250449542 1103 98-99 2.035919035651183 1103 100-101 1.8092986469739678 1104 1 0.20935966744556467 1104 2 0.22507641514903298 1104 3 0.20020927256516075 1104 4 0.17309118808256585 1104 5 0.19113126034761052 1104 6 0.13919723522870697 1104 7 0.14447919076116733 1104 8 0.2637856863774033 1104 9 0.14383167549129183 1104 10-11 0.19059450937276523 1104 12-13 0.22264465112907317 1104 14-15 0.3782569152091355 1104 16-17 0.25729019746899695 1104 18-19 0.35258652322998785 1104 20-21 0.31682087307481055 1104 22-23 0.2795406971102281 1104 24-25 0.33154793104140623 1104 26-27 0.45387810042992527 1104 28-29 0.27446020255382564 1104 30-31 0.32165232009413813 1104 32-33 0.37175478154767205 1104 34-35 0.40505608058902 1104 36-37 0.41683111614466384 1104 38-39 0.4720219422314855 1104 40-41 0.46874592167836937 1104 42-43 0.4791598690695196 1104 44-45 0.5234977861518288 1104 46-47 0.6620889465415232 1104 48-49 0.6993771694299511 1104 50-51 0.6504293858618624 1104 52-53 0.7082730175452312 1104 54-55 0.9898054311858004 1104 56-57 1.1246902191318142 1104 58-59 1.0908172776573934 1104 60-61 0.9136394927181257 1104 62-63 1.07686518033913 1104 64-65 1.2087692952839042 1104 66-67 1.1299950427288117 1104 68-69 1.3533478642273806 1104 70-71 1.5118149784480188 1104 72-73 1.5308650387094502 1104 74-75 1.604197736794987 1104 76-77 1.7209139504026894 1104 78-79 1.8436865265260565 1104 80-81 1.9136763651350357 1104 82-83 1.9350520806455798 1104 84-85 2.256554083122637 1104 86-87 2.058684723805076 1104 88-89 2.0034753245955095 1104 90-91 2.3491640977797665 1104 92-93 2.383780952142157 1104 94-95 2.070154334945041 1104 96-97 2.2318417117961324 1104 98-99 2.2095366603247157 1104 100-101 1.9947638495497078 1105 1 0.12963319865894007 1105 2 0.14686276926588704 1105 3 0.1615049667844488 1105 4 0.11259356057786363 1105 5 0.06100117161338403 1105 6 0.02497108574897311 1105 7 0.14423858274470547 1105 8 0.09150608582680775 1105 9 0.15588513458241948 1105 10-11 0.23479758554626784 1105 12-13 0.21722282517488622 1105 14-15 0.33593170941409056 1105 16-17 0.2755859307870949 1105 18-19 0.25593616276909614 1105 20-21 0.231932861068465 1105 22-23 0.31725512647322063 1105 24-25 0.28671075249121003 1105 26-27 0.2864719400626754 1105 28-29 0.42396725688011117 1105 30-31 0.45533250492120203 1105 32-33 0.5156643159410876 1105 34-35 0.5391792598630474 1105 36-37 0.5554849944952593 1105 38-39 0.45642289844784045 1105 40-41 0.7102913068667434 1105 42-43 0.7199891947755788 1105 44-45 0.6138351937809077 1105 46-47 0.8286285420994943 1105 48-49 0.836184506570909 1105 50-51 0.796643493912903 1105 52-53 0.8104394421000833 1105 54-55 0.7658177894150455 1105 56-57 1.0173495943730515 1105 58-59 1.0601417606258536 1105 60-61 0.9979086909821433 1105 62-63 1.1436583416577868 1105 64-65 1.3169558076005323 1105 66-67 1.422487922235323 1105 68-69 1.5375525995936492 1105 70-71 1.6229886643522988 1105 72-73 1.7883171201191743 1105 74-75 1.5972529372866298 1105 76-77 1.663168402402892 1105 78-79 1.8990518095963722 1105 80-81 2.007529281454577 1105 82-83 2.161170607162088 1105 84-85 2.282313928251277 1105 86-87 2.470242480255628 1105 88-89 2.278938043887159 1105 90-91 2.320995916786398 1105 92-93 2.182676260586593 1105 94-95 2.4663948848145623 1105 96-97 2.5646844303459098 1105 98-99 2.501056330531327 1105 100-101 2.506710538833527 1106 1 0.031852987510603725 1106 2 0.08301561705706462 1106 3 0.08495571993866946 1106 4 0.06673319240900355 1106 5 0.027679916525812587 1106 6 -0.023050906294336926 1106 7 0.07404835785009567 1106 8 0.014343789092720272 1106 9 -0.103431230061652 1106 10-11 0.10508345619174264 1106 12-13 0.013776363369892408 1106 14-15 0.1269954606215009 1106 16-17 0.002847760905488883 1106 18-19 0.09894791737768571 1106 20-21 0.0477258236544813 1106 22-23 0.1954012436744037 1106 24-25 0.12977446681593818 1106 26-27 -0.11354498111106892 1106 28-29 0.09687822708388438 1106 30-31 0.2418794989332511 1106 32-33 0.12568795574286895 1106 34-35 0.10725969599570817 1106 36-37 0.01902547223556894 1106 38-39 0.10339662502359914 1106 40-41 0.10285668141032289 1106 42-43 0.08984455985925877 1106 44-45 0.18115442630224976 1106 46-47 -0.25954086601329607 1106 48-49 -0.041915258077743545 1106 50-51 0.28862848964148924 1106 52-53 0.3076766056827225 1106 54-55 -0.008527891972455848 1106 56-57 0.23015815035646625 1106 58-59 0.45137935882598157 1106 60-61 0.5474322814408978 1106 62-63 0.5124371313278466 1106 64-65 0.5558239679823558 1106 66-67 0.5590208609572507 1106 68-69 0.4562046175246124 1106 70-71 0.4678815600510795 1106 72-73 0.7783161830998324 1106 74-75 0.6944689431719091 1106 76-77 0.9095948947307946 1106 78-79 1.1368463850518538 1106 80-81 1.2062445432069389 1106 82-83 1.4148153881138796 1106 84-85 1.6675224549279157 1106 86-87 1.6509700857425713 1106 88-89 1.533909728956953 1106 90-91 1.6573980715321532 1106 92-93 1.5203309310797515 1106 94-95 1.6366975271094226 1106 96-97 1.5646820628768268 1106 98-99 1.840410220806362 1106 100-101 1.7510578948939006 1107 1 0.015992872266011204 1107 2 0.024713184383536202 1107 3 0.0025753645609896125 1107 4 0.02830056709859008 1107 5 -0.026883094299464005 1107 6 -0.010108552700778262 1107 7 -0.05111257471493502 1107 8 0.007084103001638198 1107 9 0.07996077032410653 1107 10-11 0.056836234840140776 1107 12-13 0.03891595880832455 1107 14-15 0.08484016973739017 1107 16-17 -0.01617989297497502 1107 18-19 0.08471171418073453 1107 20-21 0.2029422086389303 1107 22-23 0.12701056932476718 1107 24-25 0.14704379680605228 1107 26-27 0.1315596551933922 1107 28-29 0.13379242049227713 1107 30-31 0.16999001801611513 1107 32-33 0.1282220107613128 1107 34-35 0.15365504763637716 1107 36-37 0.07850614827289348 1107 38-39 0.08694871413323568 1107 40-41 0.17606363948346626 1107 42-43 0.305362919432028 1107 44-45 0.33139805530145594 1107 46-47 0.2512637968118838 1107 48-49 0.22678811969788626 1107 50-51 0.25517187513224115 1107 52-53 0.13444939542161904 1107 54-55 0.1594188013756579 1107 56-57 0.3487935386439531 1107 58-59 0.3978869413415964 1107 60-61 0.35833651752354 1107 62-63 0.5424914988086194 1107 64-65 0.6619119203414918 1107 66-67 0.6597571726480673 1107 68-69 0.7766086968939021 1107 70-71 0.8660675426438438 1107 72-73 1.2107850212510982 1107 74-75 1.1366503931321255 1107 76-77 1.2299373736950443 1107 78-79 1.3407053912480578 1107 80-81 1.4191226443232203 1107 82-83 1.5377330292161737 1107 84-85 1.6619361058669924 1107 86-87 1.9594048809302649 1107 88-89 1.810817653830977 1107 90-91 2.102429052557781 1107 92-93 2.0679905978830426 1107 94-95 1.8778110358009847 1107 96-97 2.0256096854146186 1107 98-99 2.1431284742962475 1107 100-101 2.066895708621992 1108 1 -6.790755757357658E-4 1108 2 -0.01056042987791983 1108 3 -0.008485923665787709 1108 4 -0.026219624847428236 1108 5 -0.08377799131654484 1108 6 -0.009529979073981565 1108 7 0.043580414240189214 1108 8 -0.01913818507696874 1108 9 -0.004747378263083135 1108 10-11 0.013888883216473857 1108 12-13 -0.07359142153886467 1108 14-15 0.09525415307319918 1108 16-17 0.005113007074037057 1108 18-19 -0.11650504576135745 1108 20-21 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6019.0 12 8523.0 13 12243.0 14 16308.0 15 21729.0 16 27089.0 17 33591.0 18 34438.0 19 33159.0 20 35175.0 21 38548.0 22 42368.0 23 46995.0 24 53453.0 25 60156.0 26 69600.0 27 81267.0 28 94514.0 29 110281.0 30 127402.0 31 147653.0 32 172183.0 33 204174.0 34 245484.0 35 294384.0 36 334570.0 37 290786.0 38 90473.0 39 1778.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.27106627863723 19.20654544393631 14.83082062391049 23.691567653515968 2 16.038279731280486 20.36097652405483 39.2117823507597 24.388961393904985 3 19.504439531078177 23.65297276484778 29.697130347782714 27.14545735629133 4 13.47466918390615 16.774243652301557 35.97946101760792 33.77162614618438 5 13.995252818411188 37.03341257456211 34.191338398938264 14.77999620808844 6 33.18369905133178 35.8222429318402 16.776467748598364 14.21759026822966 7 29.375025294537803 32.07566156838166 20.11640409569156 18.432909041388974 8 26.580476219297434 35.5854808023959 19.152320024968226 18.681722953338443 9 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28.48244362537504 22.949513960620255 34-35 22.826166028270304 26.639843627075884 28.17121092203834 22.362779422615475 36-37 22.849933467716575 26.23508583931583 28.245930446554766 22.669050246412827 38-39 22.36327854362149 26.563286274178044 28.32687418592134 22.746560996279122 40-41 22.767187670644194 26.304662890486334 28.434594148737652 22.493555290131823 42-43 22.560376758924498 26.305742157069545 28.234403791071045 22.899477292934915 44-45 22.53760125658986 26.59149271455714 28.27914204489727 22.59176398395573 46-47 22.841681993797685 26.838713370526808 27.60899855269589 22.71060608297962 48-49 22.6849254034456 26.850821840042265 27.6183902866058 22.845862469906336 50-51 22.81193588623128 26.953549566538214 27.574983948223036 22.659530599007468 52-53 23.08549973073851 26.73247074857612 27.54587016309189 22.63615935759348 54-55 22.607902396445528 26.848397210472797 27.72006065584589 22.823639737235784 56-57 22.665929433435167 27.082765915323186 27.471290015958804 22.780014635282843 58-59 22.668463444789726 26.693931899629813 27.54344553352242 23.094159122058038 60-61 22.465997760590252 26.934353063255124 27.811048070742306 22.78860110541232 62-63 22.46648997862315 26.898749292208702 27.84209426741003 22.792666461758124 64-65 22.272969321491654 26.847427457504697 27.671072749682747 23.208530471320902 66-67 22.212383879142607 26.991357510863956 27.57844954397548 23.217809066017956 68-69 22.595591241988373 26.694412292908204 27.677948498434635 23.032047966668788 70-71 22.384854706351838 26.82232788502662 27.43481119063167 23.358006217989878 72-73 22.435087705225705 26.862415486450033 27.340013778486494 23.36248302983777 74-75 22.44124101656514 27.1981272379969 27.080614740216436 23.28001700522152 76-77 22.432654546435668 26.888540325600406 27.42299795784207 23.255807170121855 78-79 22.479232956586003 26.95807068032189 27.428193592633793 23.13450277045831 80-81 22.486083882096473 27.131535069856778 27.22363466722184 23.15874638082491 82-83 22.553330584174397 27.142803493109053 27.227411229329807 23.07645469338674 84-85 22.488282177953376 27.050744199520842 27.159987172337168 23.300986450188613 86-87 22.532223151809745 27.024057315907484 27.121193033720935 23.32252649856183 88-89 22.386221978665446 27.207242386754306 26.839136219335273 23.567399415244978 90-91 22.257326236043117 27.155101848837056 27.111147896390452 23.47642401872938 92-93 22.453158438481534 26.770109416841542 27.127789223410055 23.648942921266872 94-95 22.22572649116113 26.705367164639487 27.187102864290175 23.881803479909205 96-97 22.336329726346776 26.52093121105894 27.14087082136438 24.001868241229904 98-99 22.116067199793488 26.724212423801912 26.869125058747134 24.290595317657466 100-101 22.398896118823643 26.46295885439331 27.083974052448212 24.054170974334834 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 380.0 1 261.5 2 231.0 3 349.5 4 327.0 5 303.5 6 528.0 7 1179.5 8 1829.0 9 2012.0 10 1773.5 11 1558.5 12 1657.0 13 1803.0 14 2001.0 15 2214.5 16 2408.0 17 2627.5 18 2893.0 19 3238.5 20 3673.5 21 4244.0 22 5218.0 23 6486.0 24 8011.0 25 10118.0 26 12934.5 27 16463.5 28 19864.0 29 23534.5 30 27854.0 31 34019.0 32 42213.5 33 46450.5 34 48450.0 35 52954.0 36 58807.5 37 64866.0 38 70148.5 39 74491.5 40 79113.5 41 85017.0 42 89648.5 43 93774.0 44 98746.0 45 104333.5 46 106343.0 47 106546.0 48 108008.0 49 106682.5 50 104377.5 51 101836.0 52 98776.0 53 97774.0 54 97078.0 55 90776.5 56 82045.0 57 74872.0 58 68036.0 59 59553.0 60 48569.5 61 40082.5 62 34050.0 63 26220.0 64 19422.0 65 15027.5 66 11187.5 67 8745.5 68 6862.0 69 5023.0 70 3730.0 71 2646.5 72 2024.5 73 1600.5 74 1239.0 75 1006.0 76 700.5 77 431.5 78 244.5 79 145.0 80 85.0 81 49.5 82 30.0 83 18.5 84 11.5 85 6.0 86 2.5 87 1.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022860793083570963 2 0.012688287070307329 3 0.002005332726629032 4 0.0 5 2.5522416520733134E-4 6 0.0 7 0.0 8 3.6460595029618764E-5 9 2.187635701777126E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016589570738476537 26-27 0.008477088344386362 28-29 1.8230297514809383E-4 30-31 0.0 32-33 2.916847602369501E-4 34-35 0.009789669765452638 36-37 0.0012214299334922286 38-39 0.01177677219456686 40-41 0.011722081302022432 42-43 0.005013331816572581 44-45 9.479754707700878E-4 46-47 5.469089254442815E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.469089254442815E-5 66-67 6.015998179887096E-4 68-69 0.0011849693384626098 70-71 0.0 72-73 2.0053327266290321E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.4584238011847506E-4 82-83 0.001093817850888563 84-85 0.006435295022727713 86-87 0.004831028841424486 88-89 0.0 90-91 0.0016042661813032257 92-93 3.8283624781099704E-4 94-95 1.8230297514809382E-5 96-97 1.8230297514809382E-5 98-99 0.0 100-101 1.8230297514809382E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2742687.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.01213978811259 #Duplication Level Percentage of deduplicated Percentage of total 1 76.69917477783098 42.19385724510918 2 13.316427696801366 14.651303638694621 3 4.23116483235118 6.982962936715471 4 1.8527859850056505 4.07702886418347 5 1.0228633912005352 2.813495193043337 6 0.6464259381034873 2.133676444176652 7 0.41023650825947644 1.5797591696990267 8 0.2945564888032313 1.2963346190031186 9 0.22201254020023437 1.0992046406588334 >10 1.1594139875121117 11.652686699522622 >50 0.09201294842240378 3.4762958294695014 >100 0.047818864221269866 4.679959589299539 >500 0.0030503623279263064 1.1890582221004447 >1k 0.0019230545110293382 1.7238152239903979 >5k 1.3262444903650608E-4 0.45056168433371574 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6789 0.24753097965608178 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5562 0.20279382955473957 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3923 0.14303491430119442 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.8230297514809382E-5 0.0 0.0 0.0 0.0 12-13 3.6460595029618764E-5 0.0 0.0 0.0 0.0 14-15 3.6460595029618764E-5 0.0 0.0 1.8230297514809382E-5 0.0 16-17 3.6460595029618764E-5 0.0 0.0 7.292119005923753E-5 0.0 18-19 3.6460595029618764E-5 0.0 0.0 9.115148757404691E-5 0.0 20-21 3.6460595029618764E-5 3.6460595029618764E-5 0.0 1.8230297514809383E-4 0.0 22-23 3.6460595029618764E-5 3.6460595029618764E-5 0.0 4.3752714035542515E-4 0.0 24-25 3.6460595029618764E-5 3.6460595029618764E-5 0.0 7.109816030775659E-4 0.0 26-27 3.6460595029618764E-5 3.6460595029618764E-5 0.0 0.001093817850888563 0.0 28-29 3.6460595029618764E-5 3.6460595029618764E-5 0.0 0.0031538414700620233 0.0 30-31 3.6460595029618764E-5 3.6460595029618764E-5 0.0 0.010281887798352492 0.0 32-33 3.6460595029618764E-5 3.6460595029618764E-5 0.0 0.025522416520733136 0.0 34-35 3.6460595029618764E-5 3.6460595029618764E-5 0.0 0.04967756072785556 0.0 36-37 3.6460595029618764E-5 3.6460595029618764E-5 0.0 0.09142494203676906 0.0 38-39 3.6460595029618764E-5 3.6460595029618764E-5 0.0 0.15519452274357226 0.0 40-41 3.6460595029618764E-5 3.6460595029618764E-5 0.0 0.22552701055570687 0.0 42-43 3.6460595029618764E-5 3.6460595029618764E-5 0.0 0.2963517164007413 0.0 44-45 3.6460595029618764E-5 3.6460595029618764E-5 0.0 0.3901648274119504 0.0 46-47 3.6460595029618764E-5 3.6460595029618764E-5 0.0 0.4986350976250662 0.0 48-49 3.6460595029618764E-5 3.6460595029618764E-5 0.0 0.6137229658360579 0.0 50-51 3.6460595029618764E-5 3.6460595029618764E-5 0.0 0.7417543452825641 0.0 52-53 7.292119005923753E-5 3.6460595029618764E-5 0.0 0.8662672773087122 0.0 54-55 7.292119005923753E-5 3.6460595029618764E-5 0.0 1.0027028239095457 0.0 56-57 7.292119005923753E-5 3.6460595029618764E-5 0.0 1.1645331749485086 0.0 58-59 7.292119005923753E-5 3.6460595029618764E-5 0.0 1.3381402981820383 0.0 60-61 7.292119005923753E-5 3.6460595029618764E-5 0.0 1.533368554268132 0.0 62-63 7.292119005923753E-5 3.6460595029618764E-5 0.0 1.724622605495997 0.0 64-65 7.292119005923753E-5 3.6460595029618764E-5 0.0 1.920598303780198 0.0 66-67 7.292119005923753E-5 3.6460595029618764E-5 0.0 2.1504276645494 0.0 68-69 7.292119005923753E-5 3.6460595029618764E-5 0.0 2.40484969666608 0.0 70-71 7.292119005923753E-5 3.6460595029618764E-5 0.0 2.677830171652835 0.0 72-73 7.292119005923753E-5 3.6460595029618764E-5 0.0 2.961329528305636 0.0 74-75 1.093817850888563E-4 3.6460595029618764E-5 0.0 3.2443731275205665 0.0 76-77 1.093817850888563E-4 3.6460595029618764E-5 0.0 3.5462850846633245 0.0 78-79 1.093817850888563E-4 3.6460595029618764E-5 0.0 3.892733658634762 0.0 80-81 1.093817850888563E-4 3.6460595029618764E-5 0.0 4.26548855192007 0.0 82-83 1.093817850888563E-4 3.6460595029618764E-5 0.0 4.64655646087213 0.0 84-85 1.093817850888563E-4 3.6460595029618764E-5 0.0 5.028918720947742 0.0 86-87 1.093817850888563E-4 3.6460595029618764E-5 0.0 5.425774067547628 0.0 88-89 1.093817850888563E-4 3.6460595029618764E-5 0.0 5.852271877906593 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4490 0.0 52.48313 1 GTATCAA 7345 0.0 39.39217 1 ATCAACG 9335 0.0 30.42709 3 TATCAAC 9530 0.0 30.006048 2 TCAACGC 9580 0.0 29.698523 4 CAACGCA 9685 0.0 29.27846 5 AACGCAG 10165 0.0 27.98936 6 ACGCAGA 11650 0.0 24.380846 7 CGCAGAG 11800 0.0 23.950163 8 GTACATG 8825 0.0 23.256985 1 TACATGG 8745 0.0 22.9766 2 GTGGTAT 2115 0.0 21.115524 1 ACATGGG 9060 0.0 21.022758 3 GAGTACT 7875 0.0 21.019663 12-13 GCAGAGT 13165 0.0 20.781416 9 GTACTTT 8220 0.0 19.935211 14-15 CAGAGTA 12720 0.0 19.267977 10-11 TGGTATC 2160 0.0 19.132456 2 AGTACTT 8395 0.0 18.670965 12-13 AGAGTAC 12255 0.0 18.60379 10-11 >>END_MODULE