FastQCFastQC Report
Thu 26 May 2016
SRR938579_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938579_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3205017
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150200.468640259942459No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112900.3522602220206632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86210.2689845326873461No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71450.051.779281
GTATCAA137550.044.4589351
TATCAAC179850.033.7908482
ATCAACG181250.033.5036053
TCAACGC185150.032.823574
CAACGCA187600.032.3695565
AACGCAG193850.031.4004826
ACGCAGA224550.026.8183387
CGCAGAG224300.026.742288
GTACATG176600.025.122891
TACATGG177250.024.467372
GCAGAGT241050.024.2329489
GGACCGA8900.023.4917666
ACATGGG184800.022.6185913
TGGTATC36600.022.6072032
AGTACTT129100.022.38196212-13
TAGCCCT22200.022.0629144
GAGTACT124450.021.84348712-13
GTGTAGC18000.021.663071
GTGGTAT40500.021.4869481
Produced by FastQC (version 0.11.5)