FastQCFastQC Report
Thu 26 May 2016
SRR938579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938579_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3205017
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149370.4660505700905798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118680.37029444773615866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88070.2747879340421595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75850.052.4466321
GTATCAA140600.043.2685551
TATCAAC190700.031.8513582
ATCAACG190550.031.7832373
TCAACGC195200.031.0017724
CAACGCA200050.030.2501665
AACGCAG205800.029.4835016
ACGCAGA235600.025.713967
CGCAGAG236450.025.4809678
GTACATG173650.023.3738821
GCAGAGT253700.023.3554219
TACATGG175400.022.8697072
GCGCGAC1252.264175E-422.7895039
GAGTACT131300.022.09378212-13
AGTACTT137800.022.05079812-13
TAAGGTG13950.021.4517775
ACATGGG179900.021.2814273
AGAGTAC236750.021.03677210-11
GGACCGA8550.020.546096
GTACTTT142050.020.48862614-15
Produced by FastQC (version 0.11.5)