##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938579_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3205017 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82042903360575 31.0 31.0 34.0 30.0 34.0 2 32.03129624585455 31.0 31.0 34.0 30.0 34.0 3 32.08822137292876 33.0 31.0 34.0 30.0 34.0 4 35.76548423924117 37.0 35.0 37.0 35.0 37.0 5 35.66612876000346 37.0 35.0 37.0 33.0 37.0 6 35.67087382063808 37.0 35.0 37.0 33.0 37.0 7 35.61169815947934 37.0 35.0 37.0 33.0 37.0 8 35.59060154751129 37.0 35.0 37.0 33.0 37.0 9 37.173194089142115 39.0 37.0 39.0 33.0 39.0 10-11 37.07621987652483 39.0 37.0 39.0 33.0 39.0 12-13 36.96833339729555 39.0 37.0 39.0 33.0 39.0 14-15 37.994658218661556 40.0 37.0 41.0 33.0 41.0 16-17 37.921069061412155 40.0 37.0 41.0 33.0 41.0 18-19 38.179009970929954 40.0 37.5 41.0 33.0 41.0 20-21 38.162820353214975 40.0 37.0 41.0 33.0 41.0 22-23 38.05089146173016 40.0 37.0 41.0 33.0 41.0 24-25 37.92983297748499 40.0 37.0 41.0 33.0 41.0 26-27 37.74491320950872 40.0 37.0 41.0 32.0 41.0 28-29 37.62949104482129 40.0 37.0 41.0 32.0 41.0 30-31 37.37428896632997 39.0 36.0 41.0 32.0 41.0 32-33 37.163139228278666 39.0 36.0 41.0 31.0 41.0 34-35 37.05178334467493 39.0 36.0 41.0 31.0 41.0 36-37 37.00670682870013 39.0 36.0 41.0 31.0 41.0 38-39 36.84644340420035 39.0 35.0 41.0 30.5 41.0 40-41 36.66719973716208 39.0 35.0 41.0 30.0 41.0 42-43 36.44182058940717 39.0 35.0 40.5 30.0 41.0 44-45 36.10437729347457 39.0 35.0 40.0 29.5 41.0 46-47 35.67257568368592 38.0 35.0 40.0 28.0 41.0 48-49 35.29551637323608 38.0 34.5 40.0 27.0 41.0 50-51 34.92106282119565 38.0 34.0 40.0 26.0 41.0 52-53 34.448845201133096 38.0 33.0 40.0 24.0 41.0 54-55 34.12360012442992 38.0 33.0 40.0 24.0 41.0 56-57 33.806966858522124 37.0 33.0 40.0 23.0 41.0 58-59 33.35013948443955 37.0 32.0 40.0 22.0 41.0 60-61 32.97248797744287 36.0 31.5 39.0 21.0 40.5 62-63 32.506944736954594 36.0 31.0 39.0 19.5 40.0 64-65 32.00230856809808 35.0 31.0 39.0 18.0 40.0 66-67 31.512223804117106 35.0 30.0 38.0 14.5 40.0 68-69 31.042735030734626 35.0 29.5 38.0 12.0 40.0 70-71 30.499768799978284 34.0 29.0 37.0 8.0 39.0 72-73 30.013475591549124 34.0 29.0 36.5 7.0 39.0 74-75 29.428301004331644 34.0 28.0 36.0 6.0 39.0 76-77 28.179583134816447 31.5 26.5 34.5 2.0 37.0 78-79 28.87343187259225 33.5 28.0 35.0 2.0 37.0 80-81 28.500875814387257 33.0 27.0 35.0 2.0 37.0 82-83 28.069992608463544 33.0 26.5 35.0 2.0 36.0 84-85 27.52469113268354 33.0 26.0 35.0 2.0 36.0 86-87 26.916573609437954 32.0 25.5 35.0 2.0 35.5 88-89 26.194988981337698 32.0 24.0 34.0 2.0 35.0 90-91 25.6101075282908 31.5 22.0 34.0 2.0 35.0 92-93 24.802911497817327 31.0 19.0 34.0 2.0 35.0 94-95 24.009728185529127 31.0 12.0 34.0 2.0 35.0 96-97 22.96833370930638 30.0 2.0 34.0 2.0 35.0 98-99 21.630309761227476 30.0 2.0 34.0 2.0 35.0 100-101 17.685623196382423 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29103048996053005 1101 2 0.2605413244243948 1101 3 0.23563963895661288 1101 4 0.16645476338970155 1101 5 0.06243292560940006 1101 6 0.1701594005403635 1101 7 0.16205751691924775 1101 8 0.24819113901021694 1101 9 0.17408404892140084 1101 10-11 0.16512429777086624 1101 12-13 0.30619166382277285 1101 14-15 0.38491003799102685 1101 16-17 0.3506457851663072 1101 18-19 0.3839995959282234 1101 20-21 0.3073878119340634 1101 22-23 0.33804570003136547 1101 24-25 0.37889647444708174 1101 26-27 0.32878592522817485 1101 28-29 0.31136928073480874 1101 30-31 0.38646097675614755 1101 32-33 0.2995760984924729 1101 34-35 0.46046195750957253 1101 36-37 0.46883579811616016 1101 38-39 0.5186667303665544 1101 40-41 0.5147425507543417 1101 42-43 0.6513784228137638 1101 44-45 0.6114545049436728 1101 46-47 0.6838925425501401 1101 48-49 0.7335514759888113 1101 50-51 0.8408327018767068 1101 52-53 0.8515563114314091 1101 54-55 0.8488203300618267 1101 56-57 0.7125347940765536 1101 58-59 0.807101154125462 1101 60-61 0.7158029716961849 1101 62-63 0.720975505914403 1101 64-65 0.7529027418055421 1101 66-67 0.7659671213978889 1101 68-69 0.7303485341352065 1101 70-71 0.6784492311745254 1101 72-73 0.7920557365845973 1101 74-75 0.6998886111591034 1101 76-77 0.5894182765875406 1101 78-79 0.7482093231723965 1101 80-81 0.7096697330053949 1101 82-83 0.7790209595347797 1101 84-85 0.9311620353404741 1101 86-87 1.1283382971832339 1101 88-89 0.9735935932320245 1101 90-91 1.060070274018802 1101 92-93 1.380169493813007 1101 94-95 1.0825231576626955 1101 96-97 1.2385813633984561 1101 98-99 1.4393748504200765 1101 100-101 1.7755156359326882 1102 1 0.29341837917568725 1102 2 0.32918857881645636 1102 3 0.29850515649629017 1102 4 0.27252463482023614 1102 5 0.15250395077143963 1102 6 0.11644996327818546 1102 7 0.17273618777831956 1102 8 0.23129211598767085 1102 9 0.20181766176921911 1102 10-11 0.43179621145301894 1102 12-13 0.3667582657736972 1102 14-15 0.6227908663372119 1102 16-17 0.4953314151282626 1102 18-19 0.46418112507221565 1102 20-21 0.5050133232599308 1102 22-23 0.44577583883481253 1102 24-25 0.39766038697366923 1102 26-27 0.361287916378771 1102 28-29 0.3712966608166042 1102 30-31 0.4304693057430953 1102 32-33 0.34323031129903825 1102 34-35 0.4133502215497984 1102 36-37 0.6291645519369737 1102 38-39 0.6815049879015476 1102 40-41 0.5335225829385948 1102 42-43 0.591035545648765 1102 44-45 0.714478047904457 1102 46-47 0.768063314088586 1102 48-49 0.746361613039177 1102 50-51 0.8631790218793327 1102 52-53 0.7263319269441908 1102 54-55 0.7122278008175513 1102 56-57 0.7350547015669235 1102 58-59 0.7424700464674956 1102 60-61 0.8191996187615018 1102 62-63 0.9694417864588587 1102 64-65 0.8623183313616565 1102 66-67 0.890957570070487 1102 68-69 0.934581556234857 1102 70-71 0.9235438070990298 1102 72-73 1.028877715674703 1102 74-75 1.0435057315434584 1102 76-77 1.038237617790326 1102 78-79 1.1865222042359385 1102 80-81 1.271470664756027 1102 82-83 1.246363336244638 1102 84-85 1.0801566620447716 1102 86-87 1.0753424272575174 1102 88-89 1.0306871260253772 1102 90-91 1.0348674474224921 1102 92-93 1.5296736393759218 1102 94-95 1.54108651640734 1102 96-97 1.4817798430795435 1102 98-99 1.5513263891073699 1102 100-101 1.6372293953282693 1103 1 0.27993590929674994 1103 2 0.28678219917654246 1103 3 0.2799056919118925 1103 4 0.19310425538598963 1103 5 0.15232727426395343 1103 6 0.18614249294588348 1103 7 0.1788021535955977 1103 8 0.18596693619733884 1103 9 0.29371930800985524 1103 10-11 0.30128043224284085 1103 12-13 0.3749443496808169 1103 14-15 0.4681611328429156 1103 16-17 0.5027376031062047 1103 18-19 0.4922880010026347 1103 20-21 0.3900135312104567 1103 22-23 0.3502225161382668 1103 24-25 0.4317054178735944 1103 26-27 0.5003592038835762 1103 28-29 0.45317313689886873 1103 30-31 0.3551449002019993 1103 32-33 0.43429429154166854 1103 34-35 0.552802118248664 1103 36-37 0.5262348493149176 1103 38-39 0.44013485344171244 1103 40-41 0.5102829981924515 1103 42-43 0.6309377231134192 1103 44-45 0.7058744340825953 1103 46-47 0.4726146399170901 1103 48-49 0.6627002915879459 1103 50-51 0.7194117987703521 1103 52-53 0.5882330660678647 1103 54-55 0.606776250087556 1103 56-57 0.6162802001894008 1103 58-59 0.6879495440112251 1103 60-61 0.7050782140931346 1103 62-63 0.6999384429075306 1103 64-65 0.7275158392998833 1103 66-67 0.6839684450687677 1103 68-69 0.5172010075256246 1103 70-71 0.5496272537916447 1103 72-73 0.6752271088337949 1103 74-75 0.5460454970599642 1103 76-77 0.6644268857838291 1103 78-79 0.913353827615051 1103 80-81 0.9912343591128341 1103 82-83 0.948496517754176 1103 84-85 0.9121811196332352 1103 86-87 0.9193249633222713 1103 88-89 0.9383034471991607 1103 90-91 0.9630664188329341 1103 92-93 1.1316719625236615 1103 94-95 0.975683142874221 1103 96-97 1.0968907773572951 1103 98-99 1.3867998392542482 1103 100-101 1.6540873963905014 1104 1 0.23941654421998848 1104 2 0.2891871803513766 1104 3 0.26619637540692764 1104 4 0.1957064183293653 1104 5 0.13055896130230593 1104 6 0.06891613525548479 1104 7 0.19568033816840824 1104 8 0.24784475124678806 1104 9 0.27147605489046356 1104 10-11 0.24638855888259314 1104 12-13 0.2962384923250383 1104 14-15 0.3670216151940693 1104 16-17 0.4048289000100098 1104 18-19 0.3201445191893413 1104 20-21 0.36279228525981466 1104 22-23 0.4227166858808644 1104 24-25 0.3572998372497622 1104 26-27 0.34800806020878383 1104 28-29 0.38527198850239586 1104 30-31 0.45630181624218125 1104 32-33 0.4204283381919396 1104 34-35 0.4804633022836171 1104 36-37 0.4248199341205279 1104 38-39 0.5149324880816479 1104 40-41 0.4194055547192619 1104 42-43 0.44341616758433133 1104 44-45 0.46586644827230117 1104 46-47 0.48026100195965427 1104 48-49 0.5426959394239717 1104 50-51 0.7492927403979763 1104 52-53 0.6459199730342036 1104 54-55 0.6252468750336746 1104 56-57 0.6389363956590444 1104 58-59 0.8165931982687553 1104 60-61 0.8415536592661326 1104 62-63 0.6271388005240297 1104 64-65 0.6834429939289848 1104 66-67 0.826512644167952 1104 68-69 0.849104986881585 1104 70-71 0.9205382725675371 1104 72-73 0.6416439537612639 1104 74-75 0.7597079559504891 1104 76-77 0.7594506379239867 1104 78-79 0.9577154657531466 1104 80-81 0.8647309636037761 1104 82-83 0.9635641918510025 1104 84-85 1.0928519174341602 1104 86-87 1.195404997975352 1104 88-89 1.2263765163369769 1104 90-91 1.391929272292284 1104 92-93 1.5780789124368226 1104 94-95 1.6986617474719132 1104 96-97 1.6437753362079945 1104 98-99 1.8409897722062851 1104 100-101 2.006939860084831 1105 1 0.1893341493006453 1105 2 0.18235303984386064 1105 3 0.19627022493703095 1105 4 0.13341595798352301 1105 5 0.08069543097111165 1105 6 0.08667673015666821 1105 7 0.194921712851162 1105 8 0.1474967190545513 1105 9 0.2908975734831003 1105 10-11 0.1741383783045123 1105 12-13 0.27107010079227223 1105 14-15 0.37459953019804004 1105 16-17 0.2436531886507609 1105 18-19 0.20329273531466896 1105 20-21 0.16045532852356104 1105 22-23 0.16540608565114923 1105 24-25 0.3310202984244839 1105 26-27 0.16727381786706985 1105 28-29 0.17091968164201177 1105 30-31 0.2808242217396497 1105 32-33 0.2959413575759626 1105 34-35 0.37274452650436984 1105 36-37 0.35866544808720846 1105 38-39 0.3390844124717276 1105 40-41 0.3444164584780438 1105 42-43 0.30637195456928623 1105 44-45 0.2556266839908332 1105 46-47 0.24649260371688086 1105 48-49 0.27357380548437504 1105 50-51 0.3553200520935178 1105 52-53 0.40763801071286565 1105 54-55 0.39056305443553896 1105 56-57 0.2387055912083369 1105 58-59 0.19889649643376828 1105 60-61 0.28256106790151847 1105 62-63 0.3644717411246958 1105 64-65 0.4239246539053809 1105 66-67 0.15146049361121428 1105 68-69 0.456478542750272 1105 70-71 0.49028374627418714 1105 72-73 0.4284689074819603 1105 74-75 0.41794907604774423 1105 76-77 0.3831898476432336 1105 78-79 0.7334274664004639 1105 80-81 0.632396384972381 1105 82-83 0.6406598350839623 1105 84-85 0.795476368299159 1105 86-87 0.9113703704003875 1105 88-89 0.8074899726607647 1105 90-91 0.9799232964404112 1105 92-93 1.1756933574996395 1105 94-95 1.1204230844207537 1105 96-97 1.0706646467746737 1105 98-99 1.3762153764028007 1105 100-101 1.4483005801078832 1106 1 0.043388727616648026 1106 2 0.10465364490637086 1106 3 0.11994758118679982 1106 4 0.07422729554652108 1106 5 0.04988539790289792 1106 6 -0.04430511796812908 1106 7 0.08309101202710423 1106 8 0.055747284860977686 1106 9 -0.17375299406911893 1106 10-11 0.08686142145322862 1106 12-13 0.058146921956705455 1106 14-15 0.046245535898052026 1106 16-17 0.07086921735157858 1106 18-19 0.09701224759849936 1106 20-21 0.23983669733139834 1106 22-23 0.22656017073233414 1106 24-25 0.21633285101041366 1106 26-27 0.23803281560772405 1106 28-29 0.20870250605264573 1106 30-31 0.18584342302998635 1106 32-33 0.06520755743716933 1106 34-35 0.1775482679311935 1106 36-37 0.28959172182452875 1106 38-39 0.2112002302711531 1106 40-41 0.42769812120941 1106 42-43 0.2568734091537124 1106 44-45 0.2808087677006057 1106 46-47 -0.27917607109584 1106 48-49 0.09697867265625604 1106 50-51 0.3872671778647572 1106 52-53 0.3701920757174335 1106 54-55 0.5373430618163582 1106 56-57 0.5355036141955694 1106 58-59 0.2838912178368389 1106 60-61 0.4544294196456846 1106 62-63 0.23150643050217212 1106 64-65 0.31493008962360847 1106 66-67 0.2843361868968515 1106 68-69 0.05369507094166792 1106 70-71 0.18610562890747673 1106 72-73 0.544612879091499 1106 74-75 0.5946827083964976 1106 76-77 0.5042091866571816 1106 78-79 0.7272926553509578 1106 80-81 0.12056078623109912 1106 82-83 0.5182084129664197 1106 84-85 0.7491300933106864 1106 86-87 1.0763325133798034 1106 88-89 1.2604865523627282 1106 90-91 1.1138764345262757 1106 92-93 1.1323274408932278 1106 94-95 1.4017150773929679 1106 96-97 1.416280356189489 1106 98-99 1.4330732121732161 1106 100-101 1.429433560500211 1107 1 0.11478651953010655 1107 2 0.1613871907942297 1107 3 0.11772886386384585 1107 4 0.061100113830732994 1107 5 0.04459695090421434 1107 6 0.05080673892689447 1107 7 0.0535628262523673 1107 8 0.10634989435115472 1107 9 0.16006947738586064 1107 10-11 0.15602395418182624 1107 12-13 0.14618133636105313 1107 14-15 0.28994414042474403 1107 16-17 0.2616044458354807 1107 18-19 0.19751560591721784 1107 20-21 0.18971563472984343 1107 22-23 0.25021168823051454 1107 24-25 0.2912749086714044 1107 26-27 0.21779176264719524 1107 28-29 0.2351220589916423 1107 30-31 0.18657955879606192 1107 32-33 0.2293416849020744 1107 34-35 0.20034593209198448 1107 36-37 0.2719510403914569 1107 38-39 0.22888590923439267 1107 40-41 0.34549748537872205 1107 42-43 0.30034024432437434 1107 44-45 0.2722587787437831 1107 46-47 0.49932349496666717 1107 48-49 0.4475250123225365 1107 50-51 0.4833316810452857 1107 52-53 0.41028299638889365 1107 54-55 0.3352033880163745 1107 56-57 0.31873488592108146 1107 58-59 0.2951970076717956 1107 60-61 0.3023243417064734 1107 62-63 0.41935132374072026 1107 64-65 0.3556027248017841 1107 66-67 0.3544669455038907 1107 68-69 0.4024701025361672 1107 70-71 0.5801701247564353 1107 72-73 0.5675134143879852 1107 74-75 0.5630633746715361 1107 76-77 0.469994934262278 1107 78-79 0.7906137113540943 1107 80-81 0.7904465786504353 1107 82-83 0.8253089499125075 1107 84-85 0.9591434184448779 1107 86-87 1.0958527352332084 1107 88-89 1.1095785370287459 1107 90-91 1.2082231042966391 1107 92-93 1.363329410337066 1107 94-95 1.49820934822678 1107 96-97 1.7163405667339404 1107 98-99 1.5572044436667198 1107 100-101 1.5595098271898493 1108 1 0.19861447568976232 1108 2 0.1955137773442246 1108 3 0.1667737207027855 1108 4 0.14794348614323383 1108 5 0.059306478252871386 1108 6 0.20251445626876574 1108 7 0.12344673597519318 1108 8 0.06205718411248995 1108 9 0.13034397795755837 1108 10-11 0.1688647545217279 1108 12-13 0.20472805286885176 1108 14-15 0.32352115440205154 1108 16-17 0.3291139947434303 1108 18-19 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-1.3299180617807025 2316 56-57 -1.1748602461648119 2316 58-59 -0.9622195257298287 2316 60-61 -1.1021900318435627 2316 62-63 -1.33299705824124 2316 64-65 -1.2308290788338425 2316 66-67 -1.1616619454684134 2316 68-69 -1.062136136707597 2316 70-71 -1.15856307766688 2316 72-73 -1.069658192404514 2316 74-75 -1.1722891089866785 2316 76-77 -1.418343475068017 2316 78-79 -1.525796612144724 2316 80-81 -1.3583013220873958 2316 82-83 -1.4026535250956904 2316 84-85 -1.574115304473473 2316 86-87 -1.7466426348059052 2316 88-89 -1.6718609072089414 2316 90-91 -1.4578906495566102 2316 92-93 -1.8792245843150859 2316 94-95 -2.0442520717676125 2316 96-97 -2.031427383605177 2316 98-99 -2.3265911926186824 2316 100-101 -2.4017177399517458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 19.0 4 52.0 5 122.0 6 284.0 7 651.0 8 1438.0 9 2722.0 10 4462.0 11 6791.0 12 9609.0 13 13509.0 14 17612.0 15 23917.0 16 30408.0 17 38923.0 18 36067.0 19 28636.0 20 27371.0 21 29084.0 22 31584.0 23 35783.0 24 41417.0 25 48800.0 26 57663.0 27 68923.0 28 83159.0 29 97909.0 30 117115.0 31 140182.0 32 167801.0 33 206085.0 34 261641.0 35 342646.0 36 449723.0 37 517165.0 38 256590.0 39 9145.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.163878734628724 19.79470228430292 15.690276829607702 22.351142151460653 2 15.677468738839758 21.26514358780971 39.4647642332868 23.592623440063733 3 18.803550807998835 25.46014576521747 30.069388087489084 25.66691533929461 4 13.05849547755909 17.859842865108046 36.72442299058008 32.35723866675278 5 13.716839297208175 37.83745988064551 33.636516307140226 14.809184515006091 6 31.334467180673304 36.74701257434828 17.506334599785276 14.412185645193146 7 28.42050447782336 33.075425184952216 20.46291798140228 18.04115235582214 8 26.56045194144056 34.936881770049894 19.664700686455017 18.83796560205453 9 26.773368128780596 16.585528251488213 19.955837987754823 36.685265631976364 10-11 24.849992995356967 27.18397437517492 27.03536361897612 20.930669010491986 12-13 26.09302852371766 24.411274573582606 27.561273465944176 21.934423436755562 14-15 22.669130928166684 25.757585685192936 26.32716144719357 25.24612193944681 16-17 21.87961873525164 29.071608668534367 26.85815707061772 22.19061552559628 18-19 21.91620200454475 27.095082490982108 29.758375696603167 21.230339807869974 20-21 23.018037033812924 26.12178032129003 29.66804856261293 21.19213408228412 22-23 22.4423770607145 26.27218513973561 29.551013301957525 21.734424497592368 24-25 22.15229252566302 26.39240877606944 29.845425900974977 21.609872797292567 26-27 21.834918090254156 26.856384033438164 29.541534131231884 21.767163745075795 28-29 21.79585202995245 26.89049515704068 29.687675262522294 21.625977550484578 30-31 21.926723970491395 26.58727626695281 29.610321615207162 21.875678147348637 32-33 21.682035604641307 26.733792958416423 29.471200265459927 22.11297117148234 34-35 22.30916180602449 26.76313996028274 29.18602394269331 21.74167429099946 36-37 22.344984640697174 26.447664615118622 29.208157173900762 21.999193570283442 38-39 21.921445641331104 26.685666804684967 29.321561526779867 22.071326027204062 40-41 22.324628664408724 26.406363836336205 29.304333717630506 21.964673781624562 42-43 22.166449582391635 26.461769688482896 29.080384832774904 22.291395896350565 44-45 22.085789067707122 26.813264959697246 28.917894845890967 22.18305112670466 46-47 22.22627836295408 26.997111091766442 28.372564014481043 22.404046530798432 48-49 22.237775961874775 26.944256457922066 28.344795051009093 22.473172529194073 50-51 22.43068832346983 27.100535959856444 28.04910144235682 22.419674274316908 52-53 22.629099939251493 27.09009031777366 27.944188751572923 22.33662099140192 54-55 22.233589997522596 27.155701495741297 27.97539817895104 22.635310327785067 56-57 22.27442163333299 27.299964399564807 27.893767802167662 22.53184616493454 58-59 22.296643819007592 26.979852178360918 27.927697660650626 22.795806341980864 60-61 22.221161385415428 27.142102522389116 28.043454995714534 22.593281096480926 62-63 22.14869111385327 27.16984682296153 28.016902100218417 22.66455996296678 64-65 21.985407491478444 26.96858594467005 28.02220675102457 23.02379981282694 66-67 21.972362714564806 27.168192346420263 27.91951824777888 22.939926691236053 68-69 22.19501368012002 26.904080007052656 28.018414916050794 22.88249139677653 70-71 22.095784917008164 26.94996028098473 27.852624044699958 23.101630757307145 72-73 22.19931195351287 27.031799529237922 27.670033472531806 23.098855044717407 74-75 22.053980520774445 27.274099015209472 27.436881014503854 23.235039449512225 76-77 21.989550533071405 27.011843915419064 27.848918826365722 23.14968672514381 78-79 22.08880653403957 27.08871134115538 27.743810043691973 23.078672081113076 80-81 21.947061049494213 27.41987960972641 27.5446391997909 23.08842014098848 82-83 22.150074176867744 27.290891617498936 27.449904473678217 23.109129731955107 84-85 22.13894631725946 27.00690440711817 27.494207319451704 23.35994195617067 86-87 22.07748865910217 27.026356129545626 27.42652307380262 23.46963213754959 88-89 22.067479828016047 27.038632372511362 27.311079262230876 23.58280853724172 90-91 21.998922033767123 27.007875957108553 27.57173281667521 23.421469192449116 92-93 21.924921903855957 26.756529312015843 27.562340766803562 23.756208017324635 94-95 21.811062928828427 26.81839482309123 27.554798828956745 23.815743419123596 96-97 21.9582607110574 26.660095287084808 27.507949954304152 23.87369404755365 98-99 21.908082422727325 26.64622014284887 27.250937349130922 24.19476008529288 100-101 21.84205270630822 26.59214242249302 27.614532461656083 23.951272409542682 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2016.0 1 1305.5 2 741.5 3 924.0 4 841.0 5 829.0 6 1354.0 7 2955.5 8 4501.5 9 4804.0 10 4025.0 11 3180.5 12 3236.0 13 3523.5 14 3895.0 15 4189.0 16 4427.5 17 4699.5 18 4930.5 19 5305.0 20 5838.0 21 6491.0 22 7552.0 23 8913.0 24 10370.0 25 12620.5 26 15458.0 27 19210.5 28 23376.0 29 28187.0 30 34321.5 31 40607.5 32 48145.0 33 55406.5 34 61086.0 35 66807.0 36 73354.0 37 79200.0 38 84873.5 39 89652.5 40 93906.5 41 101370.0 42 109708.0 43 116166.0 44 118837.5 45 121291.5 46 122691.5 47 124335.5 48 129037.5 49 128718.5 50 124916.5 51 120307.5 52 113563.5 53 108810.0 54 106297.0 55 98245.0 56 86341.0 57 77062.0 58 69451.5 59 61135.5 60 50725.5 61 41390.0 62 34657.5 63 27004.0 64 20372.5 65 16008.5 66 11846.5 67 9382.5 68 7192.0 69 4880.5 70 3750.0 71 3036.0 72 2578.5 73 2198.5 74 1739.5 75 1451.5 76 1025.5 77 575.5 78 346.5 79 215.0 80 119.5 81 70.0 82 39.5 83 23.0 84 19.5 85 15.0 86 7.0 87 4.0 88 3.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.0561407318588324E-4 2 0.05244901977118998 3 0.0 4 0.0 5 0.04527277078405512 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006583428418632412 26-27 0.0017628611642309543 28-29 1.0920378893466089E-4 30-31 0.0016692579165726736 32-33 4.212146144622634E-4 34-35 0.011388395131757493 36-37 0.024024833565625393 38-39 0.003993738566753312 40-41 9.98434641688328E-4 42-43 0.0012636438433867902 44-45 0.008517895536903549 46-47 0.0 48-49 0.0 50-51 6.084211097788249E-4 52-53 0.0 54-55 0.001591255210190773 56-57 0.0 58-59 0.023400811914570187 60-61 0.0 62-63 0.010187153453476221 64-65 0.015475736946169084 66-67 0.006739433831396214 68-69 0.01714499486274176 70-71 9.360324765828076E-5 72-73 3.120108255276025E-5 74-75 0.022823591887344123 76-77 0.0499217320844164 78-79 0.031076278222549207 80-81 0.026661325041333634 82-83 0.037066886072679175 84-85 0.016910986743596056 86-87 0.011060783764953508 88-89 0.08553776781839223 90-91 0.02605290393155481 92-93 0.02475805900561526 94-95 0.027004536949413998 96-97 0.038221326127131305 98-99 0.05308864196352156 100-101 0.014570905552139038 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3205017.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.390241989778765 #Duplication Level Percentage of deduplicated Percentage of total 1 71.85580233975682 34.77119663590543 2 14.739928166464722 14.265373817743676 3 5.45403971101768 7.917669043140258 4 2.632997886827876 5.096456196087082 5 1.4446766560224276 3.495412649095482 6 0.8748096111654555 2.5399349267568394 7 0.6240245014948245 2.1137687644419927 8 0.42378196013266406 1.6405529281377913 9 0.28948382722001037 1.2607373206173242 >10 1.4707951547195004 12.654117962399644 >50 0.11076503543249704 3.706315497968029 >100 0.07199809914416215 6.516434282407994 >500 0.004898839680205464 1.5800096819674783 >1k 0.0018048356706843955 1.3312256082956673 >5k 6.445841681015697E-5 0.2747042792066342 >10k+ 1.2891683362031394E-4 0.8360904058287532 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14937 0.4660505700905798 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11868 0.37029444773615866 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8807 0.2747879340421595 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.120108255276025E-5 0.0 0.0 0.0 0.0 3 3.120108255276025E-5 0.0 0.0 0.0 0.0 4 3.120108255276025E-5 0.0 0.0 0.0 0.0 5 3.120108255276025E-5 0.0 0.0 0.0 0.0 6 3.120108255276025E-5 0.0 0.0 0.0 0.0 7 3.120108255276025E-5 0.0 0.0 0.0 0.0 8 3.120108255276025E-5 0.0 0.0 0.0 0.0 9 3.120108255276025E-5 0.0 0.0 0.0 0.0 10-11 6.24021651055205E-5 0.0 0.0 0.0 0.0 12-13 7.800270638190062E-5 0.0 0.0 4.680162382914038E-5 0.0 14-15 1.872064953165615E-4 0.0 0.0 1.24804330211041E-4 0.0 16-17 2.0280703659294162E-4 0.0 0.0 1.5600541276380124E-4 0.0 18-19 2.964102842512224E-4 0.0 0.0 2.652092016984621E-4 0.0 20-21 3.74412990633123E-4 0.0 0.0 4.212146144622634E-4 0.0 22-23 3.74412990633123E-4 0.0 0.0 7.800270638190063E-4 0.0 24-25 4.0561407318588324E-4 0.0 0.0 0.0013104454672159304 0.0 26-27 4.0561407318588324E-4 0.0 0.0 0.0025272876867735803 0.0 28-29 6.24021651055205E-4 0.0 0.0 0.006692632207567073 0.0 30-31 7.176248987134857E-4 0.0 0.0 0.020077896622701222 0.0 32-33 7.332254399898658E-4 0.0 0.0 0.0433227031245076 0.0 34-35 8.73630311477287E-4 0.0 0.0 0.07613064142873502 0.0 36-37 9.98434641688328E-4 0.0 0.0 0.1279400390075934 0.0 38-39 0.0010608368067938484 0.0 0.0 0.2033062539137858 0.0 40-41 0.0010764373480702286 0.0 0.0 0.2900920650342884 0.0 42-43 0.0011700405957285093 0.0 0.0 0.38110562284069005 0.0 44-45 0.0013416465497686908 0.0 0.0 0.4919006669855417 0.0 46-47 0.0014196492561505914 0.0 0.0 0.6235692353581901 0.0 48-49 0.0015288530450852523 0.0 0.0 0.7635684927724252 0.0 50-51 0.0015288530450852523 0.0 0.0 0.9139109090529005 0.0 52-53 0.0015288530450852523 0.0 0.0 1.063520099893386 0.0 54-55 0.0015912552101907727 0.0 0.0 1.2279966065702617 0.0 56-57 0.0016848584578490536 0.0 0.0 1.423159377937777 0.0 58-59 0.0016848584578490536 0.0 0.0 1.6345155111501748 0.0 60-61 0.0017004589991254336 0.0 0.0 1.867837206479716 0.0 62-63 0.0017160595404018138 0.0 0.0 2.105667458238131 0.0 64-65 0.0017784617055073343 0.0 0.0 2.3531388445053487 0.0 66-67 0.0018096627880600945 0.0 0.0 2.6349158210393266 0.0 68-69 0.0018252633293364745 0.0 0.0 2.9442121523848392 0.0 70-71 0.0019032660357183753 0.0 0.0 3.2839919413843983 0.0 72-73 0.0020280703659294164 0.0 0.0 3.6279994770698565 0.0 74-75 0.002121673613587697 0.0 0.0 3.984830657684499 0.0 76-77 0.002121673613587697 0.0 0.0 4.365281057791581 0.0 78-79 0.002121673613587697 0.0 0.0 4.78944417455508 0.0 80-81 0.00246488552166806 0.0 0.0 5.236196875086778 0.0 82-83 0.00246488552166806 0.0 0.0 5.703932303635208 0.0 84-85 0.0025740893106027205 0.0 0.0 6.181792483472007 0.0 86-87 0.0026832930995373816 0.0 0.0 6.668825781579318 0.0 88-89 0.0026832930995373816 0.0 0.0 7.200679434773669 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7585 0.0 52.446632 1 GTATCAA 14060 0.0 43.268555 1 TATCAAC 19070 0.0 31.851358 2 ATCAACG 19055 0.0 31.783237 3 TCAACGC 19520 0.0 31.001772 4 CAACGCA 20005 0.0 30.250166 5 AACGCAG 20580 0.0 29.483501 6 ACGCAGA 23560 0.0 25.71396 7 CGCAGAG 23645 0.0 25.480967 8 GTACATG 17365 0.0 23.373882 1 GCAGAGT 25370 0.0 23.355421 9 TACATGG 17540 0.0 22.869707 2 GCGCGAC 125 2.264175E-4 22.789503 9 GAGTACT 13130 0.0 22.093782 12-13 AGTACTT 13780 0.0 22.050798 12-13 TAAGGTG 1395 0.0 21.451777 5 ACATGGG 17990 0.0 21.281427 3 AGAGTAC 23675 0.0 21.036772 10-11 GGACCGA 855 0.0 20.54609 6 GTACTTT 14205 0.0 20.488626 14-15 >>END_MODULE