FastQCFastQC Report
Thu 26 May 2016
SRR938578_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938578_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172384
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149930.47260987320576575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112820.3556316007141632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86220.27178298717935784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76500.052.285041
GTATCAA139300.042.8653221
TATCAAC184250.032.421282
ATCAACG187550.031.8254783
TCAACGC191400.031.1604824
CAACGCA194650.030.6128925
AACGCAG200300.029.7442136
ACGCAGA226900.026.191117
CGCAGAG228100.025.9283668
GTACATG172100.024.7905851
TACATGG172100.024.382722
GCAGAGT241700.023.8404929
TGGTATC35800.022.6994532
GAGTACT126500.022.56771912-13
ACATGGG179950.022.4739823
CGTTAGG1302.9335386E-421.9336172
AGTACTT129650.021.67134712-13
GTGTAGC17100.021.4414561
TAGGACG5350.021.3186574
GTGGTAT41850.021.0492151
Produced by FastQC (version 0.11.5)