##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938578_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3172384 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.07977060784571 31.0 31.0 34.0 28.0 34.0 2 31.178298402715434 31.0 31.0 34.0 28.0 34.0 3 31.236925920695604 31.0 31.0 34.0 27.0 34.0 4 34.87356448651865 37.0 35.0 37.0 32.0 37.0 5 34.901418302450146 37.0 35.0 37.0 32.0 37.0 6 34.93964160706901 37.0 35.0 37.0 32.0 37.0 7 34.86507434156773 37.0 35.0 37.0 32.0 37.0 8 34.83016904637017 37.0 35.0 37.0 32.0 37.0 9 36.3331544983205 39.0 35.0 39.0 32.0 39.0 10-11 36.18251778473224 39.0 35.0 39.0 31.5 39.0 12-13 36.02905527830175 38.5 35.0 39.0 30.0 39.0 14-15 36.99813452595903 39.0 36.0 41.0 31.0 41.0 16-17 36.86491105742558 39.0 36.0 41.0 31.0 41.0 18-19 36.885063882556466 39.0 36.0 41.0 30.5 41.0 20-21 36.98828136820763 39.0 36.0 41.0 31.0 41.0 22-23 36.90453031537166 39.0 36.0 41.0 30.0 41.0 24-25 36.791718940708314 39.0 36.0 41.0 30.0 41.0 26-27 36.6070117930238 39.0 36.0 41.0 30.0 41.0 28-29 36.422528451788935 39.0 35.5 41.0 30.0 41.0 30-31 36.193453093950794 39.0 35.0 40.0 29.0 41.0 32-33 35.90678697786901 38.0 35.0 40.0 28.5 41.0 34-35 35.65140695451748 38.0 35.0 40.0 27.0 41.0 36-37 35.36903350918426 38.0 34.0 40.0 26.5 41.0 38-39 35.118900328585696 38.0 34.0 40.0 25.5 41.0 40-41 35.02189031970909 38.0 34.0 40.0 25.0 41.0 42-43 34.82109274917538 38.0 34.0 40.0 24.5 41.0 44-45 34.49551819704046 38.0 33.0 40.0 24.0 41.0 46-47 34.076073388341385 38.0 33.0 40.0 23.0 41.0 48-49 33.682720629028516 38.0 33.0 40.0 20.5 41.0 50-51 32.93577747838849 37.5 31.5 39.5 18.5 40.5 52-53 32.99642161856824 37.0 32.0 39.5 18.5 40.0 54-55 33.122659016058584 37.0 32.0 40.0 17.5 41.0 56-57 32.841350857903706 37.0 32.0 40.0 14.5 41.0 58-59 32.525802992323754 37.0 31.0 40.0 12.5 41.0 60-61 32.092360193469645 36.0 31.0 39.0 8.0 41.0 62-63 31.58215320087354 36.0 30.5 39.0 7.5 40.5 64-65 31.100798484672726 35.0 29.5 39.0 4.0 40.0 66-67 30.570266083803222 35.0 29.0 38.0 2.0 40.0 68-69 30.031612661014556 34.5 28.5 38.0 2.0 40.0 70-71 29.424702999384692 34.0 28.0 37.0 2.0 39.5 72-73 28.87826048170713 34.0 26.5 36.5 2.0 39.0 74-75 28.283050853868886 34.0 26.0 36.0 2.0 39.0 76-77 27.55237197010198 33.0 25.5 35.5 2.0 38.0 78-79 26.78099719327799 32.5 24.0 35.0 2.0 37.0 80-81 26.127695291616654 32.0 21.5 35.0 2.0 36.5 82-83 25.476438066766192 31.5 20.0 35.0 2.0 36.0 84-85 24.809225490987217 31.0 18.0 34.0 2.0 36.0 86-87 24.132337856955527 31.0 10.5 34.0 2.0 35.0 88-89 23.347870875656916 30.0 4.5 34.0 2.0 35.0 90-91 22.521697247243715 29.5 2.0 34.0 2.0 35.0 92-93 21.677042880054874 29.0 2.0 34.0 2.0 35.0 94-95 20.71731385607795 29.0 2.0 34.0 2.0 35.0 96-97 19.475741429789082 26.5 2.0 33.5 2.0 35.0 98-99 18.004177457710036 24.5 2.0 33.0 2.0 34.5 100-101 14.576435261305063 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6055620428597308 1101 2 0.6085588569293208 1101 3 0.6251701887339927 1101 4 0.639404987951572 1101 5 0.613885268868799 1101 6 0.5130023655162503 1101 7 0.4925901416203473 1101 8 0.5805126993980281 1101 9 0.610218413644688 1101 10-11 0.6958033543953093 1101 12-13 0.64829108901273 1101 14-15 0.8525291201686187 1101 16-17 0.8522984275291279 1101 18-19 0.7528019897983782 1101 20-21 0.8701944729505229 1101 22-23 0.9595275972587629 1101 24-25 0.9902614382928334 1101 26-27 0.9121956339859949 1101 28-29 0.9772305490850357 1101 30-31 0.9788968953111876 1101 32-33 0.9942347948806969 1101 34-35 1.0109482190560257 1101 36-37 0.7494215196200784 1101 38-39 0.8572847491720452 1101 40-41 0.8372394431979515 1101 42-43 0.8476793827355138 1101 44-45 0.9539688040402723 1101 46-47 0.9511490199989154 1101 48-49 0.9792214372753492 1101 50-51 0.9774501142972909 1101 52-53 1.0163922940094992 1101 54-55 0.9588709574661394 1101 56-57 0.8780079072560056 1101 58-59 0.9038730188845179 1101 60-61 0.8533412047779834 1101 62-63 0.917817967895818 1101 64-65 0.7990463061453177 1101 66-67 0.8270412401376817 1101 68-69 0.9039395454398829 1101 70-71 1.1561441663409155 1101 72-73 1.0410310932077422 1101 74-75 1.064062157138462 1101 76-77 1.3008822153621473 1101 78-79 1.2271793088691503 1101 80-81 1.188969830942824 1101 82-83 1.303930822008045 1101 84-85 1.420821929201697 1101 86-87 1.4016694340758882 1101 88-89 1.5553483993349566 1101 90-91 1.6033887317822888 1101 92-93 1.5579600610319275 1101 94-95 1.3613016037434171 1101 96-97 1.3727519919170206 1101 98-99 1.2669873703918597 1101 100-101 1.2090232091610496 1102 1 0.5581857708091533 1102 2 0.5946425469342849 1102 3 0.5799862791228172 1102 4 0.6585357072934954 1102 5 0.5010928161331876 1102 6 0.5175927213413303 1102 7 0.4343622125459561 1102 8 0.485663486076632 1102 9 0.6233098129602936 1102 10-11 0.6238088332725482 1102 12-13 0.7259185017302769 1102 14-15 0.7840043676567419 1102 16-17 0.8216479594412434 1102 18-19 0.8691173792160143 1102 20-21 0.7385130932683168 1102 22-23 0.8307509486278661 1102 24-25 0.6855348743429772 1102 26-27 0.6333455704528319 1102 28-29 0.5749690979893387 1102 30-31 0.6439007519356963 1102 32-33 0.7895840267601955 1102 34-35 0.7885607375529702 1102 36-37 0.6888564032929949 1102 38-39 0.8062606461937847 1102 40-41 0.7779645029269986 1102 42-43 1.180494620764371 1102 44-45 1.2342880607040954 1102 46-47 1.2392844604984745 1102 48-49 1.033469160387014 1102 50-51 1.142936541282367 1102 52-53 1.1343766596129612 1102 54-55 1.152416282023502 1102 56-57 1.2369972317517863 1102 58-59 1.1870634707343513 1102 60-61 1.1669316201560775 1102 62-63 1.0727501448902395 1102 64-65 1.09627008548458 1102 66-67 1.0830431157592422 1102 68-69 0.9006641893719411 1102 70-71 0.9563457753702522 1102 72-73 0.9008128069477479 1102 74-75 0.968496815846585 1102 76-77 1.0413932064734723 1102 78-79 1.063571666081252 1102 80-81 1.272172193395349 1102 82-83 1.2606971840791452 1102 84-85 1.0277412494285123 1102 86-87 1.130325795167174 1102 88-89 1.3028816932288372 1102 90-91 1.4415544918070395 1102 92-93 1.4962677935312456 1102 94-95 1.2296396175776145 1102 96-97 1.2926701357629682 1102 98-99 1.3555203839450058 1102 100-101 1.2198884714825269 1103 1 0.5647123468233453 1103 2 0.6405260425537129 1103 3 0.539346524429444 1103 4 0.5737518982176795 1103 5 0.5696492583918271 1103 6 0.5265204899086626 1103 7 0.42426785028760605 1103 8 0.443973040193157 1103 9 0.590028624455833 1103 10-11 0.6118482825978475 1103 12-13 0.6179679177669328 1103 14-15 0.7448708371310104 1103 16-17 0.685774531298307 1103 18-19 0.7459770156391343 1103 20-21 0.6723537768084 1103 22-23 0.6810603834529303 1103 24-25 0.8300190336567326 1103 26-27 0.8286755694994028 1103 28-29 0.6788476746433716 1103 30-31 0.7183721755301846 1103 32-33 0.857541244051859 1103 34-35 0.8823128132922093 1103 36-37 0.9478375480120604 1103 38-39 0.9666765983542902 1103 40-41 1.0276684206122795 1103 42-43 1.0438631383013472 1103 44-45 1.0713522458372111 1103 46-47 0.9817317632687903 1103 48-49 1.0693962809848117 1103 50-51 1.0694918037303722 1103 52-53 1.0870461336957646 1103 54-55 1.1334947468869672 1103 56-57 1.0600966340744478 1103 58-59 1.049464044237105 1103 60-61 1.1965230197838395 1103 62-63 1.3483351136236728 1103 64-65 1.150045908810302 1103 66-67 1.3634833938890978 1103 68-69 1.3465315648287124 1103 70-71 1.371197201016198 1103 72-73 1.3569498104817548 1103 74-75 1.3283232467680186 1103 76-77 1.2496015435911474 1103 78-79 1.2502434884887954 1103 80-81 1.1419669736708684 1103 82-83 1.1608601140762964 1103 84-85 1.0816048468704338 1103 86-87 1.383210649082578 1103 88-89 1.404003236318136 1103 90-91 1.751515679154732 1103 92-93 1.691877099530565 1103 94-95 1.6118705893466583 1103 96-97 1.4839660273920963 1103 98-99 1.5553262980903995 1103 100-101 1.3209626255863114 1104 1 0.6070984302655482 1104 2 0.5906569456556348 1104 3 0.5678985496726376 1104 4 0.565450470627205 1104 5 0.5976861736708017 1104 6 0.6334228555301351 1104 7 0.5886185721968502 1104 8 0.5196056198301946 1104 9 0.5902295324477365 1104 10-11 0.5415770978104035 1104 12-13 0.6251251301048839 1104 14-15 0.6971198521207711 1104 16-17 0.7771792514923916 1104 18-19 0.8623992586355129 1104 20-21 0.8795283285203084 1104 22-23 0.83311590311407 1104 24-25 0.9237058722626585 1104 26-27 0.860584279583783 1104 28-29 0.9381108062788925 1104 30-31 0.9147770014653176 1104 32-33 0.7623017384193105 1104 34-35 0.8397296522736184 1104 36-37 0.7784743227633442 1104 38-39 0.9620579804200062 1104 40-41 0.9468531269175955 1104 42-43 0.8941736824965716 1104 44-45 0.9196525232240376 1104 46-47 0.9741074560345098 1104 48-49 0.9201508296841752 1104 50-51 0.8968720778888581 1104 52-53 0.9249139776703217 1104 54-55 0.9820432999394129 1104 56-57 0.8054044829519214 1104 58-59 0.8165058326678221 1104 60-61 0.8685683451447233 1104 62-63 0.9280888966715288 1104 64-65 0.9386790482275025 1104 66-67 0.9192836464591423 1104 68-69 1.1044427755809814 1104 70-71 1.0528193204897676 1104 72-73 1.0273151479618647 1104 74-75 0.9611400458699499 1104 76-77 1.0642318750440154 1104 78-79 1.1495487698009903 1104 80-81 1.1423932287847443 1104 82-83 1.0347437907227608 1104 84-85 1.0766936067696307 1104 86-87 1.2682630621148796 1104 88-89 1.3980009212365694 1104 90-91 1.4561690082066328 1104 92-93 1.5935213080933472 1104 94-95 1.3331944802201718 1104 96-97 1.4111398475485863 1104 98-99 1.3303492158441443 1104 100-101 1.1692813537070847 1105 1 0.4489150128373325 1105 2 0.44823810463736535 1105 3 0.4533937175990559 1105 4 0.4856654190232774 1105 5 0.387286352996135 1105 6 0.3916821413688041 1105 7 0.35022891450112326 1105 8 0.38677943381404134 1105 9 0.5379005971092212 1105 10-11 0.43048664671300685 1105 12-13 0.4859447126249421 1105 14-15 0.5925417420932249 1105 16-17 0.5475766763225138 1105 18-19 0.4476252268072827 1105 20-21 0.5130585963759273 1105 22-23 0.5915455500365638 1105 24-25 0.5998149662774779 1105 26-27 0.6921049319956367 1105 28-29 0.7019165327305146 1105 30-31 0.5964611228745369 1105 32-33 0.6705727564344315 1105 34-35 0.7768354055171542 1105 36-37 0.7410924423863676 1105 38-39 0.8866931914091154 1105 40-41 0.7541936981349338 1105 42-43 0.9319443330177748 1105 44-45 0.8347662184502198 1105 46-47 0.6770917546817756 1105 48-49 0.6682021640818547 1105 50-51 0.5102739744579452 1105 52-53 0.6596215369127663 1105 54-55 0.672515260138546 1105 56-57 0.6837362672884026 1105 58-59 0.6653120597662578 1105 60-61 0.7160147086288262 1105 62-63 0.7904048736774705 1105 64-65 0.9053107116323211 1105 66-67 0.859178501554414 1105 68-69 0.7630383065040647 1105 70-71 0.8731667764325977 1105 72-73 0.7715906575804823 1105 74-75 0.773168748410221 1105 76-77 0.7775153305778595 1105 78-79 0.8273205355595898 1105 80-81 0.8350992434334756 1105 82-83 0.9848122628707117 1105 84-85 0.8088770590868286 1105 86-87 1.000419288925933 1105 88-89 0.9249226613174883 1105 90-91 0.8899488633689572 1105 92-93 0.9392090047156998 1105 94-95 0.9126050802794019 1105 96-97 1.0330976115117174 1105 98-99 0.9512942413962939 1105 100-101 0.7368455310314452 1106 1 0.23847621634787686 1106 2 0.3573102154236061 1106 3 0.06773533586090252 1106 4 0.36789550890168954 1106 5 0.40075504456657285 1106 6 0.03595252090951817 1106 7 0.08075267149504839 1106 8 0.2722628052938987 1106 9 0.28367163943703133 1106 10-11 0.07693439863648166 1106 12-13 0.09972664073767845 1106 14-15 0.39653005698871624 1106 16-17 0.13924014711420796 1106 18-19 -0.16657824349682926 1106 20-21 0.19481049458858735 1106 22-23 0.24290881386088614 1106 24-25 0.3630630959685419 1106 26-27 0.05230712930205783 1106 28-29 0.17215382314287808 1106 30-31 0.32028984407971706 1106 32-33 0.4064202520547724 1106 34-35 0.21663833858139014 1106 36-37 0.11904170791604685 1106 38-39 0.1318041391011633 1106 40-41 0.22651309660484742 1106 42-43 -0.02846168882330602 1106 44-45 0.25846311654392906 1106 46-47 -0.09465350244231985 1106 48-49 0.22170913957464222 1106 50-51 -0.13624740415880865 1106 52-53 0.06883722954169258 1106 54-55 -0.08868904345916917 1106 56-57 -0.5417504741791959 1106 58-59 -0.8819413953278712 1106 60-61 -0.1556011437073579 1106 62-63 -0.11817373265734332 1106 64-65 -0.023908531134761546 1106 66-67 -0.43704097335215053 1106 68-69 -0.5716053437272386 1106 70-71 -0.6218865280205321 1106 72-73 -0.1327163277422514 1106 74-75 0.07555646598958177 1106 76-77 -0.02725379931617411 1106 78-79 0.19565936364390168 1106 80-81 0.03143911560696466 1106 82-83 0.10099454846454492 1106 84-85 0.10485621920794941 1106 86-87 0.24773032361889236 1106 88-89 0.1361528958057754 1106 90-91 0.2979597021581242 1106 92-93 0.24127098292200344 1106 94-95 -0.15211155926516184 1106 96-97 0.0075282945745520635 1106 98-99 0.056765948136472844 1106 100-101 -0.2531935214674199 1107 1 0.3837266889776849 1107 2 0.3475993593045956 1107 3 0.28558874695259107 1107 4 0.40486572555590783 1107 5 0.40903827358923195 1107 6 0.3281992845690169 1107 7 0.3737282080218449 1107 8 0.34165541742246575 1107 9 0.3504039389402891 1107 10-11 0.48341033492521035 1107 12-13 0.34571791954871856 1107 14-15 0.40152656365840755 1107 16-17 0.43563139483996594 1107 18-19 0.4227009487676767 1107 20-21 0.4701672931663481 1107 22-23 0.5458908647477827 1107 24-25 0.5820939183754632 1107 26-27 0.5608604257808949 1107 28-29 0.5155313513854125 1107 30-31 0.5367450762944586 1107 32-33 0.5926393133132706 1107 34-35 0.6075469393995832 1107 36-37 0.5115104057760007 1107 38-39 0.5868874662720103 1107 40-41 0.5448709284105391 1107 42-43 0.637699183506335 1107 44-45 0.6807289904984017 1107 46-47 0.7274575660802114 1107 48-49 0.6037499218774087 1107 50-51 0.4676175476242008 1107 52-53 0.3489897129862314 1107 54-55 0.4398361671332296 1107 56-57 0.5932546994872112 1107 58-59 0.5748603718663574 1107 60-61 0.5842054445273135 1107 62-63 0.6295406433235975 1107 64-65 0.6195471524938903 1107 66-67 0.6392987371671488 1107 68-69 0.5724374226140334 1107 70-71 0.5930890017322348 1107 72-73 0.6654023450067434 1107 74-75 0.6270555685245185 1107 76-77 0.7845939532013624 1107 78-79 0.8282629550471334 1107 80-81 0.6335525098802357 1107 82-83 0.6693001167370873 1107 84-85 0.6903784584467871 1107 86-87 0.7434681878006906 1107 88-89 0.8189386051588166 1107 90-91 0.6657267537591167 1107 92-93 0.7871174856307697 1107 94-95 0.6267490599603285 1107 96-97 0.5913465826902815 1107 98-99 0.8624427933496719 1107 100-101 0.6900678979969683 1108 1 0.46683465010869085 1108 2 0.4689383461523846 1108 3 0.48328643327560883 1108 4 0.4568046840495441 1108 5 0.5194387272556327 1108 6 0.4436499153406288 1108 7 0.44808426038827776 1108 8 0.28980628314391765 1108 9 0.2853971804797695 1108 10-11 0.47294473164738804 1108 12-13 0.5658235008791337 1108 14-15 0.6397363237628184 1108 16-17 0.5756795211393069 1108 18-19 0.5362300988899236 1108 20-21 0.6116691107539296 1108 22-23 0.5236296104770801 1108 24-25 0.5985525824621192 1108 26-27 0.5952809567826947 1108 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-0.8466083575208323 2316 80-81 -0.8458015975945976 2316 82-83 -0.8551636490912493 2316 84-85 -0.7983970159648024 2316 86-87 -0.8391701895538297 2316 88-89 -0.9323712882081914 2316 90-91 -0.8196451901884103 2316 92-93 -0.950192442812881 2316 94-95 -1.0661439738184804 2316 96-97 -1.1413979015076912 2316 98-99 -1.056535643284377 2316 100-101 -0.9692370583326131 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10563.0 3 3351.0 4 3598.0 5 3966.0 6 4682.0 7 5968.0 8 7375.0 9 9659.0 10 12253.0 11 15490.0 12 19398.0 13 23389.0 14 27320.0 15 32661.0 16 38143.0 17 45402.0 18 41382.0 19 36688.0 20 37903.0 21 40817.0 22 44935.0 23 50388.0 24 56555.0 25 63008.0 26 71970.0 27 81739.0 28 93422.0 29 107006.0 30 124148.0 31 145212.0 32 171468.0 33 206961.0 34 253743.0 35 314817.0 36 375994.0 37 386731.0 38 193902.0 39 10377.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33200379849052 19.00407418741262 15.125811576806218 22.538110437290644 2 15.256388746622399 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28.84848263229909 22.298076115291543 46-47 22.18758255775023 26.966490144141495 28.32694729103392 22.51898000707435 48-49 22.28658825888251 26.872929768739382 28.297245723505533 22.543236248872574 50-51 22.423941316273044 27.062932301174953 27.986982219794125 22.526144162757873 52-53 22.553774635641396 27.072685092215487 27.93511578046084 22.438424491682273 54-55 22.207890326159312 27.108295383057534 28.019259630327454 22.6645546604557 56-57 22.221453367918116 27.33625399891807 27.90051599756376 22.54177663560005 58-59 22.266601555726915 26.890072010849863 27.990442689237337 22.852883744185885 60-61 22.20052565657419 27.0254124090134 28.153632071293078 22.62042986311933 62-63 22.121141385100284 27.02949009517952 28.217646141869768 22.631722377850423 64-65 21.97144608082659 26.849015848437062 28.188239358875016 22.99129871186133 66-67 21.95415348402408 27.074164983717434 28.05187534370229 22.919806188556198 68-69 22.192725445430753 26.747502756850512 28.174602424010857 22.885169373707875 70-71 22.085353960997747 26.80700944583331 28.044861638831108 23.062774954337833 72-73 22.198959531904435 26.8580975365142 27.86462533012185 23.07831760145951 74-75 22.050938633645025 27.080830762187798 27.80626899195331 23.06196161221387 76-77 22.12455822242623 26.824073809897993 27.920279610269468 23.131088357406313 78-79 22.239689683733694 26.956723900010953 27.7965385739921 23.007047842263255 80-81 22.033188748654517 27.29452323072124 27.647449813449036 23.0248382071752 82-83 22.284087394919837 27.09946753394546 27.586035146983768 23.030409924150934 84-85 22.286817428979074 26.81543984460163 27.66099537423784 23.236747352181457 86-87 22.272677406480586 26.79535969928017 27.606078833033532 23.325884061205716 88-89 22.333918185540302 26.706483282996313 27.647754273154934 23.311844258308444 90-91 22.268099882821712 26.735853150002082 27.682676407558443 23.313370559617763 92-93 22.261008265825595 26.449459567638346 27.792283789614537 23.49724837692152 94-95 22.210623782183504 26.51526366796278 27.759365845437202 23.514746704416513 96-97 22.43615273206804 26.30211659081399 27.70585621763333 23.555874459484645 98-99 22.441180416314662 26.213150218738367 27.441729804239763 23.903939560707208 100-101 22.778725479211673 25.779181533130185 27.72986143219867 23.71223155545947 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1987.0 1 1291.5 2 765.0 3 941.0 4 808.0 5 789.0 6 1314.5 7 2903.5 8 4504.5 9 4897.5 10 4224.0 11 3412.5 12 3362.0 13 3562.0 14 3807.5 15 4138.5 16 4318.0 17 4555.5 18 4983.5 19 5328.0 20 5746.0 21 6566.0 22 7662.5 23 9054.0 24 10548.0 25 12477.0 26 15134.5 27 19002.5 28 23316.5 29 28099.0 30 33924.0 31 39977.5 32 47078.0 33 53856.0 34 59596.0 35 65566.5 36 72329.0 37 78656.5 38 84188.0 39 88455.0 40 92591.5 41 99789.0 42 107411.0 43 112809.0 44 115971.5 45 119189.5 46 120629.5 47 121846.0 48 126022.5 49 125999.5 50 122156.5 51 117533.5 52 111779.0 53 107551.5 54 104173.0 55 96402.5 56 85470.0 57 77004.5 58 69829.5 59 61376.5 60 51506.0 61 42796.5 62 35695.0 63 27932.5 64 21796.0 65 17462.5 66 13338.5 67 10373.5 68 7891.0 69 5676.0 70 4472.0 71 3691.0 72 3040.5 73 2502.5 74 1932.0 75 1547.0 76 1142.5 77 711.5 78 456.0 79 293.0 80 172.0 81 101.0 82 65.5 83 36.5 84 22.0 85 18.5 86 13.0 87 8.5 88 6.0 89 6.0 90 4.0 91 2.0 92 0.0 93 1.5 94 2.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18481369216336987 2 0.04715696460453716 3 0.04513955435407567 4 0.050687432542844746 5 0.058158154876584926 6 0.0638636432411713 7 0.034800326820460574 8 0.03423293018751828 9 0.039623198200470056 10-11 0.0409944067300806 12-13 0.03744817777419127 14-15 0.033366074220523116 16-17 0.03139594702280682 18-19 0.039607437182888325 20-21 0.05007275285715727 22-23 0.04662109000675832 24-25 0.06778813661902215 26-27 0.051743420720820686 28-29 0.04422541533433531 30-31 0.052846691951541805 32-33 0.04947383418905151 34-35 0.04871730534512846 36-37 0.04599064930348911 38-39 0.025911112904364667 40-41 0.024098595882465677 42-43 0.021135524577100377 44-45 0.004113625588831616 46-47 0.01141097672917276 48-49 0.010559881779759323 50-51 0.008227251177663233 52-53 0.00955117665452858 54-55 0.01136369367642757 56-57 0.008700081705115143 58-59 0.01141097672917276 60-61 0.011064234342374694 62-63 0.003640795061379707 64-65 0.00661962738432674 66-67 0.003956015413014314 68-69 0.00901530205674975 70-71 0.02272738735285514 72-73 0.020126819451869633 74-75 0.02036323471559559 76-77 0.012530008977475613 78-79 0.004302757799812381 80-81 0.006131035839293099 82-83 0.005138091731644088 84-85 0.0047283052745191005 86-87 0.008258773212826694 88-89 0.029236687614109767 90-91 0.036187296367652846 92-93 0.022396405983638804 94-95 0.030907355477773183 96-97 0.034831848855624035 98-99 0.021198568647427297 100-101 0.12290441510233313 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3172384.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.07943359249344 #Duplication Level Percentage of deduplicated Percentage of total 1 74.11278610085151 37.85639135993116 2 13.673600758961069 13.968795638752399 3 5.06685433407572 7.76436148440775 4 2.3927552951995152 4.888823408169227 5 1.301082428295785 3.322927674724732 6 0.8108468913886872 2.485055996542091 7 0.5369779875161231 1.9199972017772409 8 0.3559788330305762 1.4546557729694902 9 0.2669799096429558 1.2273464308623536 >10 1.3166519465382773 11.971126354828979 >50 0.0974821860546561 3.4724131231616893 >100 0.06240940681875762 6.023913173847535 >500 0.003667126400049895 1.2533895927058754 >1k 0.0017403311719997984 1.2828837265786703 >5k 6.215468471427852E-5 0.27373350550785225 >10k+ 1.2430936942855705E-4 0.8341855552329875 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14993 0.47260987320576575 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11282 0.3556316007141632 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8622 0.27178298717935784 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.304407032692133E-5 0.0 0.0 0.0 0.0 3 6.304407032692133E-5 0.0 0.0 0.0 0.0 4 6.304407032692133E-5 0.0 0.0 0.0 0.0 5 6.304407032692133E-5 0.0 0.0 0.0 0.0 6 6.304407032692133E-5 0.0 0.0 0.0 0.0 7 1.2608814065384266E-4 0.0 0.0 0.0 0.0 8 1.5761017581730332E-4 0.0 0.0 0.0 0.0 9 1.5761017581730332E-4 0.0 0.0 0.0 0.0 10-11 1.5761017581730332E-4 0.0 0.0 0.0 0.0 12-13 2.36415263725955E-4 0.0 0.0 6.304407032692133E-5 3.1522035163460665E-5 14-15 3.1522035163460664E-4 0.0 0.0 6.304407032692133E-5 3.1522035163460665E-5 16-17 3.1522035163460664E-4 0.0 0.0 7.880508790865167E-5 3.1522035163460665E-5 18-19 3.1522035163460664E-4 0.0 0.0 2.0489322856249433E-4 3.1522035163460665E-5 20-21 3.1522035163460664E-4 0.0 0.0 2.6793729888941565E-4 3.1522035163460665E-5 22-23 3.1522035163460664E-4 0.0 0.0 6.146796856874831E-4 3.1522035163460665E-5 24-25 5.043525626153706E-4 0.0 0.0 0.0013396864944470782 3.1522035163460665E-5 26-27 5.043525626153706E-4 0.0 0.0 0.002584806883403775 3.1522035163460665E-5 28-29 5.989186681057526E-4 0.0 0.0 0.006383212120600785 3.1522035163460665E-5 30-31 6.304407032692133E-4 0.0 0.0 0.018818654992586017 3.1522035163460665E-5 32-33 6.462017208509436E-4 0.0 0.0 0.04266507459374401 3.1522035163460665E-5 34-35 7.56528843923056E-4 0.0 0.0 0.07502244368903638 3.1522035163460665E-5 36-37 8.826169845768987E-4 0.0 0.0 0.12749087121861666 3.1522035163460665E-5 38-39 8.98378002158629E-4 0.0 0.0 0.2077302117272058 3.1522035163460665E-5 40-41 9.141390197403593E-4 0.0 0.0 0.2972843136265976 3.1522035163460665E-5 42-43 9.4566105490382E-4 0.0 0.0 0.3893286563039027 3.1522035163460665E-5 44-45 0.001040227160394202 0.0 0.0 0.5015313404682409 3.1522035163460665E-5 46-47 0.0011190322483028536 0.0 0.0 0.6346961780162805 3.1522035163460665E-5 48-49 0.001134793265884584 0.0 0.0 0.7786100295550602 3.1522035163460665E-5 50-51 0.001134793265884584 0.0 0.0 0.9370870613393587 3.1522035163460665E-5 52-53 0.001134793265884584 0.0 0.0 1.0939564693303208 3.1522035163460665E-5 54-55 0.001134793265884584 0.0 0.0 1.2659091711469985 3.1522035163460665E-5 56-57 0.0011663153010480446 0.0 0.0 1.4623544942856854 3.1522035163460665E-5 58-59 0.0011978373362115052 0.0 0.0 1.6782331521026457 3.1522035163460665E-5 60-61 0.0011978373362115052 0.0 0.0 1.9142701514066394 3.1522035163460665E-5 62-63 0.0011978373362115052 0.0 0.0 2.150118018499652 3.1522035163460665E-5 64-65 0.0012135983537932356 0.0 0.0 2.396289352108698 3.1522035163460665E-5 66-67 0.0012608814065384266 0.0 0.0 2.677355578643695 3.1522035163460665E-5 68-69 0.0012608814065384266 0.0 0.0 2.9876112097400567 3.1522035163460665E-5 70-71 0.0013396864944470782 0.0 0.0 3.3264258046945137 3.1522035163460665E-5 72-73 0.0014027305647739995 0.0 0.0 3.671261108365192 3.1522035163460665E-5 74-75 0.0014342525999374602 0.0 0.0 4.020604063064244 6.304407032692133E-5 76-77 0.0014500136175191905 0.0 0.0 4.397260861232436 6.304407032692133E-5 78-79 0.001465774635100921 1.5761017581730333E-5 0.0 4.80936418794194 6.304407032692133E-5 80-81 0.0016391458284999547 3.1522035163460665E-5 0.0 5.255921099085104 6.304407032692133E-5 82-83 0.0016706678636634153 3.1522035163460665E-5 0.0 5.716221617559539 6.304407032692133E-5 84-85 0.0017179509164086063 4.7283052745191E-5 0.0 6.179784666673392 7.880508790865167E-5 86-87 0.0017652339691537973 6.304407032692133E-5 0.0 6.658714707929431 9.4566105490382E-5 88-89 0.0017652339691537973 6.304407032692133E-5 0.0 7.166802631711672 9.4566105490382E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7650 0.0 52.28504 1 GTATCAA 13930 0.0 42.865322 1 TATCAAC 18425 0.0 32.42128 2 ATCAACG 18755 0.0 31.825478 3 TCAACGC 19140 0.0 31.160482 4 CAACGCA 19465 0.0 30.612892 5 AACGCAG 20030 0.0 29.744213 6 ACGCAGA 22690 0.0 26.19111 7 CGCAGAG 22810 0.0 25.928366 8 GTACATG 17210 0.0 24.790585 1 TACATGG 17210 0.0 24.38272 2 GCAGAGT 24170 0.0 23.840492 9 TGGTATC 3580 0.0 22.699453 2 GAGTACT 12650 0.0 22.567719 12-13 ACATGGG 17995 0.0 22.473982 3 CGTTAGG 130 2.9335386E-4 21.933617 2 AGTACTT 12965 0.0 21.671347 12-13 GTGTAGC 1710 0.0 21.441456 1 TAGGACG 535 0.0 21.318657 4 GTGGTAT 4185 0.0 21.049215 1 >>END_MODULE