FastQCFastQC Report
Thu 26 May 2016
SRR938578_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938578_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172384
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150220.4735240122255061No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117210.36946977415092247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88400.2786547908449923No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76050.052.9096341
GTATCAA139250.042.0461731
ATCAACG184100.031.5302053
TATCAAC184350.031.5276572
TCAACGC189300.030.6635954
CAACGCA191650.030.3124055
AACGCAG198350.029.4049286
ACGCAGA230000.025.2346537
CGCAGAG230150.025.1769338
GTACATG170150.024.067711
TACATGG172050.023.5531232
GCAGAGT248800.022.9078779
GAGTACT126000.022.22118812-13
ACATGGG179200.022.0193733
AGTACTT130950.021.72577512-13
GTGTAGC16600.021.4890291
GTACTTT134400.020.74401514-15
AGAGTAC232450.020.68804410-11
TGGTATC37900.020.4555912
TAAGGTG14300.019.9468025
Produced by FastQC (version 0.11.5)