FastQCFastQC Report
Thu 26 May 2016
SRR938577_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938577_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172017
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153630.48432905624402395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110000.34678250463348714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86860.2738320759314972No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75250.055.031841
GTATCAA140050.043.7581181
TATCAAC182600.033.391262
ATCAACG184900.032.744583
TCAACGC189250.032.0421524
CAACGCA192850.031.2961565
AACGCAG198700.030.4182666
ACGCAGA227500.026.3094087
CGCAGAG228650.026.1770868
GTACATG175200.025.595071
TACATGG178100.024.7089332
GCAGAGT243100.024.0142129
ACATGGG182950.023.1447353
GAGTACT125250.022.79210512-13
TGGTATC35550.022.4582842
GGACCGA8800.022.138416
AGTACTT129950.021.78500412-13
AGGACCG10750.021.66185
GTACTTT132700.021.49614714-15
GTGTAGC17200.021.0564881
Produced by FastQC (version 0.11.5)