FastQCFastQC Report
Thu 26 May 2016
SRR938577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938577_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172017
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150910.47575407067490494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120610.38023125348949893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90400.28499216744424766No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75350.055.550151
GTATCAA140450.043.585561
TATCAAC184150.033.0874442
ATCAACG185850.032.7196583
TCAACGC190450.031.9293674
CAACGCA194050.031.2880155
AACGCAG200200.030.3427686
ACGCAGA231000.026.1942637
CGCAGAG231300.026.0781548
GTACATG169850.023.7754081
GCAGAGT250500.023.6811859
TACATGG172850.022.8674352
GAGTACT132850.022.63032712-13
ACATGGG175300.021.7788223
GTACTTT139450.021.57629214-15
AGTACTT137850.021.1548712-13
GTAAGGT10600.021.0810764
TAAGGTG13000.020.8464585
TGGTATC34300.020.6622682
AGAGTAC235750.020.48886910-11
Produced by FastQC (version 0.11.5)