##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938577_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3172017 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.814738382549653 31.0 31.0 34.0 30.0 34.0 2 32.03849853263712 31.0 31.0 34.0 30.0 34.0 3 32.097819147879726 33.0 31.0 34.0 30.0 34.0 4 35.775930898226584 37.0 35.0 37.0 35.0 37.0 5 35.65137229718504 37.0 35.0 37.0 33.0 37.0 6 35.69315738219562 37.0 35.0 37.0 33.0 37.0 7 35.63100576068791 37.0 35.0 37.0 33.0 37.0 8 35.62023595712129 37.0 35.0 37.0 33.0 37.0 9 37.18059896904714 39.0 37.0 39.0 33.0 39.0 10-11 37.12136536468751 39.0 37.0 39.0 33.0 39.0 12-13 37.014365149997616 39.0 37.0 39.0 33.0 39.0 14-15 38.04751267095983 40.0 37.0 41.0 33.0 41.0 16-17 37.98248165126479 40.0 37.0 41.0 33.0 41.0 18-19 38.010549911933005 40.0 37.0 41.0 33.0 41.0 20-21 38.018618752673774 40.0 37.0 41.0 33.0 41.0 22-23 38.03559359864717 40.0 37.0 41.0 33.0 41.0 24-25 37.964065293471 40.0 37.0 41.0 33.0 41.0 26-27 37.799261006482624 40.0 37.0 41.0 32.0 41.0 28-29 37.6627675702873 40.0 37.0 41.0 32.0 41.0 30-31 37.40935924996619 39.0 36.0 41.0 32.0 41.0 32-33 37.17740683609199 39.0 36.0 41.0 31.0 41.0 34-35 36.999070150002346 39.0 36.0 41.0 31.0 41.0 36-37 36.99706432846986 39.0 36.0 41.0 31.0 41.0 38-39 36.85450093741616 39.0 35.0 41.0 31.0 41.0 40-41 36.67034524089877 39.0 35.0 41.0 30.0 41.0 42-43 36.428481152528505 39.0 35.0 40.5 30.0 41.0 44-45 36.10879213446839 39.0 35.0 40.0 30.0 41.0 46-47 35.69747844983176 38.0 35.0 40.0 28.5 41.0 48-49 35.278448066325 38.0 34.5 40.0 27.0 41.0 50-51 34.91119640909869 38.0 34.0 40.0 26.0 41.0 52-53 34.4117878308975 38.0 33.0 40.0 24.0 41.0 54-55 34.10138517542623 38.0 33.0 40.0 23.5 41.0 56-57 33.759496087189945 37.0 33.0 40.0 23.0 41.0 58-59 33.327160762379265 37.0 32.0 40.0 22.0 41.0 60-61 32.94022935564343 36.0 31.5 39.0 20.5 41.0 62-63 32.476413115062115 36.0 31.0 39.0 18.5 40.0 64-65 31.99122466872025 35.0 31.0 39.0 17.5 40.0 66-67 31.517296092675416 35.0 30.0 38.0 14.0 40.0 68-69 30.978765561470823 35.0 29.5 38.0 10.0 40.0 70-71 30.430760616982823 34.0 29.0 37.0 8.0 39.0 72-73 29.85847506491926 34.0 28.5 36.5 7.0 39.0 74-75 29.25715089168816 34.0 28.0 36.0 2.0 39.0 76-77 27.953802738131607 31.5 26.0 34.5 2.0 36.5 78-79 28.586796193084716 33.0 27.0 35.0 2.0 37.0 80-81 28.29561380030435 33.0 27.0 35.0 2.0 37.0 82-83 27.813082180833206 33.0 26.0 35.0 2.0 36.0 84-85 27.233129898105844 33.0 26.0 35.0 2.0 36.0 86-87 26.594130485429304 32.0 25.0 35.0 2.0 35.5 88-89 25.870636412099934 32.0 23.5 34.0 2.0 35.0 90-91 25.345361169249724 31.5 20.5 34.0 2.0 35.0 92-93 24.580902151533238 31.0 18.0 34.0 2.0 35.0 94-95 23.809471229189505 31.0 8.0 34.0 2.0 35.0 96-97 22.845428476581304 30.5 2.0 34.0 2.0 35.0 98-99 21.685436427358365 30.0 2.0 34.0 2.0 35.0 100-101 18.545709559564152 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3497413191139458 1101 2 0.4030738756450347 1101 3 0.34148547698737275 1101 4 0.20366700275943117 1101 5 0.22295039138326445 1101 6 0.20889620786224583 1101 7 0.23926184849908338 1101 8 0.2768330218768895 1101 9 0.41984291023813824 1101 10-11 0.3817195121821797 1101 12-13 0.4296915096810707 1101 14-15 0.4865815548813188 1101 16-17 0.48592803980113786 1101 18-19 0.5488878633218448 1101 20-21 0.49665425200305435 1101 22-23 0.526733059869251 1101 24-25 0.48239622392385684 1101 26-27 0.36082178070865467 1101 28-29 0.43832181760691213 1101 30-31 0.5423946727999294 1101 32-33 0.501143615658421 1101 34-35 0.3576210363453427 1101 36-37 0.49019743793093795 1101 38-39 0.6483362392200718 1101 40-41 0.7998058573412266 1101 42-43 0.6825863503084193 1101 44-45 0.7227163182291108 1101 46-47 0.818930148995257 1101 48-49 0.7975027643473567 1101 50-51 1.0337893344310274 1101 52-53 0.9842946750834329 1101 54-55 1.0295079688509006 1101 56-57 1.0695638606763396 1101 58-59 1.055412104387763 1101 60-61 1.285532939395459 1101 62-63 1.306617836909183 1101 64-65 1.3788887834727888 1101 66-67 1.307013810607927 1101 68-69 1.4319949786828943 1101 70-71 1.4878587803032666 1101 72-73 1.5907113408590448 1101 74-75 1.7470826502878687 1101 76-77 1.640576650134328 1101 78-79 1.925563348826337 1101 80-81 2.1589263547470203 1101 82-83 2.2837001365880063 1101 84-85 2.4718648369979057 1101 86-87 2.491786465936862 1101 88-89 2.6726837283175087 1101 90-91 2.824624689677915 1101 92-93 3.0838372734180517 1101 94-95 2.9993961474202955 1101 96-97 3.619278593660379 1101 98-99 3.9068121983274047 1101 100-101 4.1984246804823435 1102 1 0.45567838863989607 1102 2 0.41119506850027676 1102 3 0.35616705864033804 1102 4 0.2747473082760834 1102 5 0.27061935200231346 1102 6 0.29129305876107026 1102 7 0.36879498193493987 1102 8 0.4147744501987489 1102 9 0.516653571818587 1102 10-11 0.4621539025664845 1102 12-13 0.44362741361572944 1102 14-15 0.5510613775510649 1102 16-17 0.519262661871764 1102 18-19 0.6905032668827644 1102 20-21 0.7213559809157317 1102 22-23 0.7024201716371437 1102 24-25 0.6496875701961216 1102 26-27 0.6161202809048305 1102 28-29 0.6479621453471225 1102 30-31 0.5625043443437505 1102 32-33 0.44581311181941885 1102 34-35 0.5721338650091425 1102 36-37 0.675438746829812 1102 38-39 0.7484564497711119 1102 40-41 0.7640748998666922 1102 42-43 0.6996900421111931 1102 44-45 0.6336949996503947 1102 46-47 0.6751138685201923 1102 48-49 0.7672224496663489 1102 50-51 0.9515847201787366 1102 52-53 0.9976646085559437 1102 54-55 0.8911974604869997 1102 56-57 1.009244511574586 1102 58-59 1.1049125038963226 1102 60-61 1.2056287212150991 1102 62-63 1.3753927345977672 1102 64-65 1.3235718829718977 1102 66-67 1.3338957966380107 1102 68-69 1.3692542353964967 1102 70-71 1.589443855579841 1102 72-73 1.642025280128017 1102 74-75 1.7025288540973769 1102 76-77 1.769541307229975 1102 78-79 1.9304333438718615 1102 80-81 2.0183430149699966 1102 82-83 2.162194046588322 1102 84-85 2.262477244960582 1102 86-87 2.433786485983884 1102 88-89 2.574660937714409 1102 90-91 2.7951183333391896 1102 92-93 3.0923669243993537 1102 94-95 3.1246424394367125 1102 96-97 3.466445447909919 1102 98-99 3.7819378502136907 1102 100-101 4.339020191667149 1103 1 0.3018222830077377 1103 2 0.32767755722754544 1103 3 0.2967745289384496 1103 4 0.2109257619901186 1103 5 0.23054625329452705 1103 6 0.19588297973550084 1103 7 0.26064998942813844 1103 8 0.2425150894955408 1103 9 0.33091781531011577 1103 10-11 0.3754918497190616 1103 12-13 0.40888354873452926 1103 14-15 0.6646061401165682 1103 16-17 0.5987480790453077 1103 18-19 0.501614506723449 1103 20-21 0.5226027684503762 1103 22-23 0.4402336461247245 1103 24-25 0.4305355860834652 1103 26-27 0.5277461058436685 1103 28-29 0.46672349957916737 1103 30-31 0.5957392336314058 1103 32-33 0.5538597397899778 1103 34-35 0.5518715088445632 1103 36-37 0.5828139402933203 1103 38-39 0.6168949048072676 1103 40-41 0.6523623755623547 1103 42-43 0.7451956801256117 1103 44-45 0.8367748886915578 1103 46-47 0.8283921108981431 1103 48-49 0.8888693945842547 1103 50-51 0.967519263918355 1103 52-53 1.1543645397007722 1103 54-55 1.075685770668315 1103 56-57 1.093508218945324 1103 58-59 1.303935934874069 1103 60-61 1.216983577981118 1103 62-63 1.1571804384855255 1103 64-65 1.1195421400804406 1103 66-67 1.335594459039342 1103 68-69 1.4467174435388088 1103 70-71 1.333291415083007 1103 72-73 1.5255628050627408 1103 74-75 1.6451293988960636 1103 76-77 1.3588317150066338 1103 78-79 1.5690242048750456 1103 80-81 1.6756609048439692 1103 82-83 1.8358911651434866 1103 84-85 2.045447651774044 1103 86-87 2.3114905168626265 1103 88-89 2.457429606360634 1103 90-91 2.71759129047474 1103 92-93 3.0127743966511282 1103 94-95 2.996305229450236 1103 96-97 3.3477000296414197 1103 98-99 3.777299269461743 1103 100-101 4.144002559880821 1104 1 0.25696033762082493 1104 2 0.2852379733174075 1104 3 0.2374741784709613 1104 4 0.1301042484795829 1104 5 0.12049593593056329 1104 6 0.20157990360082323 1104 7 0.20184748164236055 1104 8 0.22036753889873495 1104 9 0.25956756535697423 1104 10-11 0.24120211817578507 1104 12-13 0.2563056221676092 1104 14-15 0.397198574197553 1104 16-17 0.43093516686337807 1104 18-19 0.4277501619755597 1104 20-21 0.40135260293273944 1104 22-23 0.4543963138564351 1104 24-25 0.41583971508232764 1104 26-27 0.40817406353649943 1104 28-29 0.463366124628827 1104 30-31 0.5073144414097897 1104 32-33 0.5723891714066838 1104 34-35 0.40853752777118046 1104 36-37 0.5839625682595226 1104 38-39 0.6593662487589569 1104 40-41 0.7537836884741083 1104 42-43 0.728858282510096 1104 44-45 0.7583712762797106 1104 46-47 0.820520188451539 1104 48-49 0.8234670856775992 1104 50-51 0.9207147432660534 1104 52-53 1.0477885344282853 1104 54-55 0.9870285551308058 1104 56-57 1.1596216088498608 1104 58-59 1.2145576427925988 1104 60-61 1.2070173736747378 1104 62-63 1.387925738644249 1104 64-65 1.2970971628745858 1104 66-67 1.4962278284282888 1104 68-69 1.5278363514146989 1104 70-71 1.4657118182925863 1104 72-73 1.6824862684141237 1104 74-75 1.8183803717958789 1104 76-77 1.8040627862368268 1104 78-79 1.9729368592793612 1104 80-81 2.246819788455401 1104 82-83 2.311316147817873 1104 84-85 2.506738663607049 1104 86-87 2.7298303447930614 1104 88-89 2.8578870799337004 1104 90-91 3.029984348879683 1104 92-93 3.2021099228424674 1104 94-95 3.2909388357228835 1104 96-97 3.58008110628856 1104 98-99 3.9381148157387535 1104 100-101 4.413279185436057 1105 1 0.2747064516104629 1105 2 0.32197031369455686 1105 3 0.2766861345513263 1105 4 0.20277487560124996 1105 5 0.09301286870299208 1105 6 0.15353732397212383 1105 7 0.16613780762868657 1105 8 0.1536987065697275 1105 9 0.2554263452228156 1105 10-11 0.26014997173368926 1105 12-13 0.3470029519059423 1105 14-15 0.420135679302561 1105 16-17 0.39043873614562585 1105 18-19 0.3700283913841744 1105 20-21 0.2418026411025309 1105 22-23 0.25127847069369835 1105 24-25 0.21840429919394921 1105 26-27 0.3314317543448837 1105 28-29 0.3211224104637722 1105 30-31 0.3991337195945306 1105 32-33 0.45281791164089924 1105 34-35 0.5460495065910109 1105 36-37 0.48272956194120553 1105 38-39 0.4652264295376227 1105 40-41 0.5023329315751184 1105 42-43 0.6265117535254134 1105 44-45 0.4992010836440457 1105 46-47 0.5015933485860273 1105 48-49 0.6106151317087551 1105 50-51 0.5913824711072664 1105 52-53 0.5878216487590109 1105 54-55 0.717420749813023 1105 56-57 0.7925029616849812 1105 58-59 0.9527947462733621 1105 60-61 1.0032874940314187 1105 62-63 1.1181829178149059 1105 64-65 1.0533240173583138 1105 66-67 1.1034078112112695 1105 68-69 1.212682535246941 1105 70-71 1.0481558426887752 1105 72-73 1.252016718980176 1105 74-75 1.4078747437808694 1105 76-77 1.4119732739981252 1105 78-79 1.6098725350472236 1105 80-81 1.895060018611165 1105 82-83 1.9072219293241979 1105 84-85 2.0703007179543462 1105 86-87 2.316991080251995 1105 88-89 2.394365631755292 1105 90-91 2.43424352993447 1105 92-93 2.71832822972765 1105 94-95 2.7698085021025953 1105 96-97 3.0655617615096986 1105 98-99 3.5458736394592556 1105 100-101 3.952680264748029 1106 1 0.09483323449477155 1106 2 0.07597723906057041 1106 3 0.03337477625554186 1106 4 0.019737551157291477 1106 5 0.056682021016328576 1106 6 0.05812525475820962 1106 7 0.08538674820665904 1106 8 -0.05314518455199391 1106 9 0.08354412612489881 1106 10-11 0.188692018243934 1106 12-13 0.0976661774175227 1106 14-15 -0.03342089855315322 1106 16-17 -0.01207406882633677 1106 18-19 0.18322583386736824 1106 20-21 0.26589188591968593 1106 22-23 0.1917044254155158 1106 24-25 0.08837359706914327 1106 26-27 0.16792629701736672 1106 28-29 0.19077127354123746 1106 30-31 0.2217469771495857 1106 32-33 0.14702648737031865 1106 34-35 0.11631365415993145 1106 36-37 0.24222511311389638 1106 38-39 0.18197895948212306 1106 40-41 0.2158123070005047 1106 42-43 0.37566843868953015 1106 44-45 -0.10584812421132739 1106 46-47 -0.17119925309612682 1106 48-49 0.17781328958126608 1106 50-51 -0.09566972747288816 1106 52-53 0.26705697706805864 1106 54-55 0.3679294711042971 1106 56-57 0.32941506319077263 1106 58-59 0.6791265378764777 1106 60-61 0.38718055326465617 1106 62-63 0.5291017956472785 1106 64-65 0.16441130975879759 1106 66-67 0.3402245274749518 1106 68-69 0.26749849637745626 1106 70-71 0.4344084446690104 1106 72-73 0.7684632212728992 1106 74-75 1.0102191341847195 1106 76-77 1.0941662900099338 1106 78-79 1.1858148369117103 1106 80-81 1.180892329368337 1106 82-83 1.4985724253771373 1106 84-85 1.5259537839440434 1106 86-87 1.7459621119526822 1106 88-89 2.0505537493374746 1106 90-91 2.093800932475098 1106 92-93 1.825517643952967 1106 94-95 2.3986260231792578 1106 96-97 2.7407171351267365 1106 98-99 3.1078136858602043 1106 100-101 3.549465732408951 1107 1 0.15741800757042412 1107 2 0.1234831357432924 1107 3 0.12205434712797114 1107 4 0.07492406033554744 1107 5 -0.011820536415939387 1107 6 0.05840914151690413 1107 7 0.10599088960783831 1107 8 0.14163054923471208 1107 9 0.10164029672998254 1107 10-11 0.13824376460895138 1107 12-13 0.22362979895888913 1107 14-15 0.2853065908275241 1107 16-17 0.314918005320699 1107 18-19 0.2697271771141416 1107 20-21 0.1629286195972739 1107 22-23 0.1906978915978499 1107 24-25 0.14511182184919846 1107 26-27 0.19609217994315742 1107 28-29 0.1593960666706593 1107 30-31 0.10578135119273924 1107 32-33 0.11736661793131731 1107 34-35 0.26361853248421596 1107 36-37 0.31571794408398546 1107 38-39 0.3685419733539277 1107 40-41 0.3846550863220486 1107 42-43 0.4255969863246776 1107 44-45 0.5445543210794241 1107 46-47 0.5302660851766205 1107 48-49 0.5204241344784393 1107 50-51 0.3834359955899558 1107 52-53 0.4674034477346183 1107 54-55 0.46499658345299366 1107 56-57 0.375149767986521 1107 58-59 0.421452678867162 1107 60-61 0.625519493673913 1107 62-63 0.599953797202744 1107 64-65 0.6900985430741997 1107 66-67 0.8534032948988255 1107 68-69 0.9214303472124143 1107 70-71 0.9177768432564015 1107 72-73 1.125411645307235 1107 74-75 1.0476820921540018 1107 76-77 0.9769576380490399 1107 78-79 1.2563001794432438 1107 80-81 1.4542506193567881 1107 82-83 1.711238072051085 1107 84-85 1.9172989390026451 1107 86-87 1.98104337249449 1107 88-89 2.308844026191956 1107 90-91 2.4397274266658933 1107 92-93 2.615871118056834 1107 94-95 2.595530042440874 1107 96-97 3.0301197856657893 1107 98-99 3.211192495871895 1107 100-101 3.522823493654709 1108 1 0.1794528176739476 1108 2 0.18353386190077003 1108 3 0.1467388337215496 1108 4 0.1269803196012944 1108 5 0.028224227991927364 1108 6 0.041697167892564835 1108 7 0.07477277171940955 1108 8 0.11728931761786043 1108 9 0.13698926107618092 1108 10-11 0.12426338979610563 1108 12-13 0.190546722346987 1108 14-15 0.2663301240828133 1108 16-17 0.27558049012503005 1108 18-19 0.2780593655144301 1108 20-21 0.2266330542986239 1108 22-23 0.28746151452411794 1108 24-25 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-0.10048123786006613 2316 60-61 0.06846462765040684 2316 62-63 0.11119960990413347 2316 64-65 0.3153160822640615 2316 66-67 0.3644946551034849 2316 68-69 0.3365888602657279 2316 70-71 0.4453154478509269 2316 72-73 0.5284131590379353 2316 74-75 0.7365092583666701 2316 76-77 0.6791012689853453 2316 78-79 0.8206794444383227 2316 80-81 0.9437364329540152 2316 82-83 1.0371910605778574 2316 84-85 1.266104729444681 2316 86-87 1.2749184889681224 2316 88-89 1.5347689236982234 2316 90-91 1.8172358362565966 2316 92-93 1.699196162308283 2316 94-95 2.118424924022481 2316 96-97 2.322004866359457 2316 98-99 2.7325350226937495 2316 100-101 3.4155818455280276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 23.0 4 39.0 5 109.0 6 248.0 7 616.0 8 1495.0 9 2501.0 10 4187.0 11 6713.0 12 9369.0 13 13488.0 14 17522.0 15 23612.0 16 30269.0 17 38402.0 18 35885.0 19 28702.0 20 27956.0 21 29741.0 22 32280.0 23 36924.0 24 42333.0 25 49704.0 26 59005.0 27 70400.0 28 84426.0 29 100329.0 30 118824.0 31 140780.0 32 168712.0 33 205704.0 34 258586.0 35 329899.0 36 418508.0 37 484915.0 38 283668.0 39 16135.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.26761222755882 19.77859499403376 15.632870588569094 22.32092218983832 2 15.695981509797688 21.21683219031182 39.45020160578122 23.636984694109277 3 18.810182546367987 25.45835203857736 30.075132029598844 25.65633338545581 4 13.104595593277086 17.82080613061027 36.73088763395656 32.34371064215608 5 13.687536410072564 37.852684609718736 33.666007524695736 14.793771455512966 6 31.365342619538296 36.68561675426078 17.5156375265328 14.43340309966813 7 28.430995168058683 33.09603952311731 20.443805944293487 18.029159364530518 8 26.56795345043863 34.87862769966239 19.684793618697505 18.86862523120147 9 26.77886026462027 16.5861343113861 19.927289166483032 36.7077162575106 10-11 24.85415431253994 27.166641919006107 27.027818577264878 20.951385191189075 12-13 26.14706982970142 24.392350356255974 27.52594011948864 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21.917478452570432 26.698444448368146 29.307310361179567 22.076766737881854 40-41 22.330636891517283 26.41314911457237 29.2854547417887 21.970759252121645 42-43 22.164749375440596 26.45080010441902 29.09578237966906 22.288668140471323 44-45 22.096953259221788 26.78523378743639 28.93009477981942 22.187718173522402 46-47 22.215046621003783 27.001578965039407 28.379126261875538 22.404248152081273 48-49 22.239824061472557 26.974051526205567 28.34179325016228 22.444331162159596 50-51 22.47940348365094 27.08376090039871 28.014730059769544 22.422105556180817 52-53 22.646946721912272 27.093691490304117 27.902750836455166 22.356610951328445 54-55 22.244146586248842 27.133954063605913 28.03883586355872 22.58306348658653 56-57 22.28377086251429 27.308428674877845 27.881738969242598 22.526061493365262 58-59 22.296586052344615 26.90357586355937 28.009622899246757 22.79021518484926 60-61 22.20035390730882 27.11443538921765 28.097185481666713 22.588025221806816 62-63 22.134165737447184 27.22337238419592 28.071681204735032 22.57078067362186 64-65 21.95595105574781 26.97688883760711 28.017551608329967 23.04960849831511 66-67 21.919310443318416 27.193901404441164 27.971405503578335 22.915382648662096 68-69 22.162948601242903 26.892343583135315 28.01500624603466 22.92970156958713 70-71 22.092220817227652 26.974477122915797 27.829390573884062 23.10391148597249 72-73 22.198954513635098 27.028784371077514 27.649257183876564 23.123003931410825 74-75 22.100023423581906 27.235698925951528 27.429518820359412 23.234758830107154 76-77 22.0054621397048 27.02562754234924 27.85279839294682 23.116111924999142 78-79 22.122926831728833 27.116910155273445 27.72562379079305 23.03453922220467 80-81 21.954492032827073 27.459681792273432 27.582726568844546 23.00309960605495 82-83 22.157313721228007 27.302622504856185 27.483045719141618 23.057018054774193 84-85 22.126567193353452 27.00151093715148 27.55500102857224 23.31692084092283 86-87 22.10460160622654 27.03070664163279 27.49210781885129 23.372583933289377 88-89 22.061434135714993 27.090556003045386 27.352235093465826 23.495774767773796 90-91 21.993947960404018 27.021913856245067 27.62276723260504 23.36137095074588 92-93 21.91511406255246 26.76829940137317 27.661408328064592 23.65517820800978 94-95 21.842899959237293 26.81243511620524 27.567758936979214 23.77690598757825 96-97 21.961373473092987 26.72859256428954 27.54721995500024 23.762814007617237 98-99 21.928145404012653 26.67143650238949 27.34329607943463 24.05712201416323 100-101 21.84370354840896 26.585797221613593 27.59295835257733 23.977540877400113 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2013.0 1 1286.5 2 739.5 3 942.5 4 806.0 5 773.5 6 1323.0 7 3051.5 8 4741.0 9 5071.0 10 4281.5 11 3446.0 12 3468.0 13 3707.0 14 3941.0 15 4215.0 16 4443.0 17 4636.5 18 4941.0 19 5338.5 20 5878.5 21 6501.0 22 7584.5 23 9027.5 24 10516.0 25 12523.0 26 15218.0 27 19065.5 28 23121.0 29 27715.5 30 33839.5 31 40125.0 32 47731.5 33 54368.0 34 59780.0 35 65798.0 36 72280.5 37 78303.5 38 83769.0 39 88466.0 40 92990.0 41 100305.0 42 108208.0 43 114503.5 44 117902.0 45 120295.0 46 121136.0 47 122956.0 48 127594.5 49 127015.5 50 123154.0 51 118717.5 52 112274.5 53 108222.0 54 105514.0 55 97093.0 56 85247.0 57 76179.0 58 68814.0 59 60537.5 60 50148.0 61 41146.0 62 34619.5 63 26863.0 64 20268.5 65 15916.5 66 11874.0 67 9345.5 68 7117.0 69 4836.5 70 3730.5 71 3085.0 72 2675.0 73 2318.0 74 1820.0 75 1411.5 76 968.5 77 575.0 78 357.0 79 216.5 80 121.0 81 72.0 82 44.0 83 26.5 84 13.0 85 5.0 86 5.0 87 4.0 88 2.5 89 2.0 90 1.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.305136447881584E-5 2 0.04643732993864787 3 3.152568223940792E-5 4 0.0 5 0.10248999296031515 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013398414951748367 26-27 0.011932470727615899 28-29 0.0 30-31 0.0 32-33 5.989879625487505E-4 34-35 0.015873181007541887 36-37 0.003499350728574279 38-39 0.01289400403591784 40-41 0.007708029307535237 42-43 0.005706148485332834 44-45 0.0027269715137087855 46-47 7.881420559851981E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.576284111970396E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.5220545791526335E-4 68-69 1.4186557007733565E-4 70-71 0.0 72-73 1.576284111970396E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.152568223940792E-5 82-83 9.615333083019417E-4 84-85 0.004555461083594445 86-87 0.0015289955886112843 88-89 6.305136447881584E-5 90-91 8.984819438231258E-4 92-93 2.364426167955594E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0010088218316610534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3172017.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.41491051223659 #Duplication Level Percentage of deduplicated Percentage of total 1 71.80732918118665 34.76545416429866 2 14.830067536658136 14.359927853554568 3 5.419139002160673 7.871013895289401 4 2.648008948103901 5.128124650322084 5 1.4449508987306168 3.4978584228309315 6 0.893967312354981 2.5968808457118633 7 0.5882548635455721 1.993621460386277 8 0.4177476879182643 1.61801735418052 9 0.2933180992859438 1.2780872575693465 >10 1.4656597719083457 12.547967535817515 >50 0.11340230238670895 3.8131327625792992 >100 0.0714200240571055 6.499376821278538 >500 0.004772904217253983 1.5700126678814228 >1k 0.001765320737300408 1.3148753212164113 >5k 6.538224952964473E-5 0.2861590087100091 >10k+ 1.3076449905928946E-4 0.8594899783732485 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15091 0.47575407067490494 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12061 0.38023125348949893 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9040 0.28499216744424766 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.152568223940792E-5 0.0 0.0 0.0 0.0 3 3.152568223940792E-5 0.0 0.0 3.152568223940792E-5 0.0 4 3.152568223940792E-5 0.0 0.0 3.152568223940792E-5 0.0 5 3.152568223940792E-5 0.0 0.0 3.152568223940792E-5 0.0 6 3.152568223940792E-5 0.0 0.0 3.152568223940792E-5 0.0 7 6.305136447881584E-5 0.0 0.0 3.152568223940792E-5 0.0 8 6.305136447881584E-5 0.0 0.0 3.152568223940792E-5 0.0 9 6.305136447881584E-5 0.0 0.0 9.457704671822377E-5 0.0 10-11 6.305136447881584E-5 0.0 0.0 1.1033988783792773E-4 0.0 12-13 6.305136447881584E-5 0.0 0.0 1.4186557007733565E-4 0.0 14-15 1.2610272895763168E-4 0.0 0.0 1.5762841119703962E-4 0.0 16-17 1.5762841119703962E-4 0.0 0.0 2.5220545791526335E-4 0.0 18-19 3.1525682239407925E-4 0.0 0.0 3.1525682239407925E-4 0.0 20-21 3.783081868728951E-4 0.0 0.0 5.044109158305267E-4 0.0 22-23 4.09833869112303E-4 0.0 0.0 9.930589905413495E-4 0.0 24-25 5.989879625487505E-4 0.0 0.0 0.001938829457723587 0.0 26-27 5.989879625487505E-4 0.0 0.0 0.0034362993640954633 0.0 28-29 6.620393270275664E-4 0.0 0.0 0.007455823849619974 0.0 30-31 6.935650092669743E-4 0.0 0.0 0.019672025717390546 0.0 32-33 6.935650092669743E-4 0.0 0.0 0.04125135521026527 0.0 34-35 8.196677382246061E-4 0.0 0.0 0.07378585928133424 0.0 36-37 9.142447849428297E-4 0.0 0.0 0.12548797815396323 0.0 38-39 9.457704671822376E-4 0.0 0.0 0.2062567760513263 0.0 40-41 9.457704671822376E-4 0.0 0.0 0.29539564258325224 0.0 42-43 9.930589905413497E-4 0.0 0.0 0.3866624926663382 0.0 44-45 0.001197975925097501 0.0 0.0 0.49995003179365055 0.0 46-47 0.0013398414951748367 0.0 0.0 0.6327519682271564 0.0 48-49 0.0014186557007733564 0.0 0.0 0.7763829765098988 0.0 50-51 0.0014186557007733564 0.0 0.0 0.9350990237441981 0.0 52-53 0.0014186557007733564 0.0 0.0 1.0915767475394993 0.0 54-55 0.0014817070652521724 0.0 0.0 1.259529819669945 0.0 56-57 0.0015762841119703961 0.0 0.0 1.4546580298907603 0.0 58-59 0.0015762841119703961 0.0 0.0 1.6672041795488486 0.0 60-61 0.0015920469530901 0.0 0.0 1.9004784652793476 0.0 62-63 0.001607809794209804 0.0 0.0 2.1394431366540596 0.0 64-65 0.001607809794209804 3.152568223940792E-5 0.0 2.3894890853359234 0.0 66-67 0.001639335476449212 3.152568223940792E-5 0.0 2.671770044107582 0.0 68-69 0.001639335476449212 3.152568223940792E-5 0.0 2.9851353255672968 0.0 70-71 0.0016550983175689159 3.152568223940792E-5 0.0 3.324540820556763 0.0 72-73 0.0016866239998083237 3.152568223940792E-5 0.0 3.673057237713417 0.0 74-75 0.0017181496820477319 3.152568223940792E-5 0.0 4.032686457859463 0.0 76-77 0.0017339125231674358 3.152568223940792E-5 0.0 4.413895007498383 0.0 78-79 0.0017339125231674358 3.152568223940792E-5 0.0 4.835046596534633 0.0 80-81 0.0017969638876462516 3.152568223940792E-5 0.0 5.292421194463964 0.0 82-83 0.0017969638876462516 3.152568223940792E-5 0.0 5.768931881512615 0.0 84-85 0.0018600152521250674 6.305136447881584E-5 0.0 6.253103309345441 0.0 86-87 0.0019230666166038834 6.305136447881584E-5 0.0 6.744478355569973 0.0 88-89 0.0019230666166038834 6.305136447881584E-5 0.0 7.2712725057904795 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7535 0.0 55.55015 1 GTATCAA 14045 0.0 43.58556 1 TATCAAC 18415 0.0 33.087444 2 ATCAACG 18585 0.0 32.719658 3 TCAACGC 19045 0.0 31.929367 4 CAACGCA 19405 0.0 31.288015 5 AACGCAG 20020 0.0 30.342768 6 ACGCAGA 23100 0.0 26.194263 7 CGCAGAG 23130 0.0 26.078154 8 GTACATG 16985 0.0 23.775408 1 GCAGAGT 25050 0.0 23.681185 9 TACATGG 17285 0.0 22.867435 2 GAGTACT 13285 0.0 22.630327 12-13 ACATGGG 17530 0.0 21.778822 3 GTACTTT 13945 0.0 21.576292 14-15 AGTACTT 13785 0.0 21.15487 12-13 GTAAGGT 1060 0.0 21.081076 4 TAAGGTG 1300 0.0 20.846458 5 TGGTATC 3430 0.0 20.662268 2 AGAGTAC 23575 0.0 20.488869 10-11 >>END_MODULE