FastQCFastQC Report
Thu 26 May 2016
SRR938576_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938576_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3155217
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142940.45302747798328924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106100.336268472184322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79740.25272429756812287No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71600.054.236071
GTATCAA137950.045.026281
TATCAAC180100.034.3027842
ATCAACG182550.033.8950043
TCAACGC186150.033.1884464
CAACGCA190000.032.4654055
AACGCAG195200.031.569196
ACGCAGA221300.027.5848057
CGCAGAG221900.027.4031738
GTACATG172600.026.4237171
TACATGG175050.025.6819932
GCAGAGT237500.025.0627549
ACATGGG183200.023.7875923
GAGTACT122150.023.31397812-13
TGGTATC34600.022.6624872
AGTACTT125550.022.30425612-13
GTACTTT129500.021.90008714-15
AGAGTAC221700.021.19065310-11
GTGGTAT38800.021.1482031
CAGAGTA232100.020.64022810-11
Produced by FastQC (version 0.11.5)