FastQCFastQC Report
Thu 26 May 2016
SRR938576_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938576_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3155217
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144500.4579716704112585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117960.37385701205337063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86960.2756070343180833No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74700.056.4812971
GTATCAA137700.043.0618741
TATCAAC182200.032.4852032
ATCAACG183350.032.0198333
TCAACGC187700.031.2767734
CAACGCA192200.030.5691995
AACGCAG197550.029.7413336
ACGCAGA225400.025.919047
GTACATG168500.025.7162271
CGCAGAG227700.025.5529388
TACATGG171450.024.9910762
ACATGGG175250.023.8509773
GCAGAGT246500.023.2186999
GAGTACT130750.022.84949912-13
TGGTATC34200.022.0844672
AGTACTT133900.021.95724312-13
GTACTTT138800.021.43874514-15
GTGGTAT38750.021.3348621
AGAGTAC228100.020.770910-11
GTGTAGC16700.020.769151
Produced by FastQC (version 0.11.5)