##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938576_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3155217 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.832303134776467 31.0 31.0 34.0 30.0 34.0 2 32.07636527059787 33.0 31.0 34.0 30.0 34.0 3 32.127916400044754 33.0 31.0 34.0 30.0 34.0 4 35.78328748862598 37.0 35.0 37.0 35.0 37.0 5 35.68290358476136 37.0 35.0 37.0 33.0 37.0 6 35.71917811041206 37.0 35.0 37.0 33.0 37.0 7 35.64497687480766 37.0 35.0 37.0 33.0 37.0 8 35.620021380462894 37.0 35.0 37.0 33.0 37.0 9 37.16124406023421 39.0 37.0 39.0 33.0 39.0 10-11 37.091383730500944 39.0 37.0 39.0 33.0 39.0 12-13 36.97489205972204 39.0 37.0 39.0 33.0 39.0 14-15 37.973662508790994 40.0 37.0 41.0 33.0 41.0 16-17 38.07238519569336 40.0 37.0 41.0 33.0 41.0 18-19 38.23356555190974 40.0 37.5 41.0 33.0 41.0 20-21 38.20228133278947 40.0 37.5 41.0 33.0 41.0 22-23 38.072539067835905 40.0 37.0 41.0 33.0 41.0 24-25 37.9541508555513 40.0 37.0 41.0 33.0 41.0 26-27 37.74585028541618 40.0 37.0 41.0 32.0 41.0 28-29 37.602308494154286 39.5 37.0 41.0 32.0 41.0 30-31 37.33358418771197 39.0 36.0 41.0 31.5 41.0 32-33 37.206074098865464 39.0 36.0 41.0 31.0 41.0 34-35 37.065578215381066 39.0 36.0 41.0 31.0 41.0 36-37 36.93762774477952 39.0 35.5 41.0 31.0 41.0 38-39 36.72622707091145 39.0 35.0 41.0 30.0 41.0 40-41 36.493319952320235 39.0 35.0 40.0 30.0 41.0 42-43 36.220195473084736 39.0 35.0 40.0 30.0 41.0 44-45 35.8818460029849 38.0 35.0 40.0 29.0 41.0 46-47 35.41340896680006 38.0 34.5 40.0 27.5 41.0 48-49 34.97720806524559 38.0 34.0 40.0 26.0 41.0 50-51 34.58028179995227 38.0 33.5 40.0 24.5 41.0 52-53 34.00197656769724 37.5 33.0 40.0 23.5 41.0 54-55 33.585555288273355 37.0 32.5 40.0 22.5 41.0 56-57 33.21486414405095 37.0 32.0 39.5 21.5 41.0 58-59 32.70190956755114 36.0 31.0 39.0 19.5 40.0 60-61 32.2438279205519 36.0 31.0 39.0 18.0 40.0 62-63 31.768066665462314 35.0 30.5 39.0 13.5 40.0 64-65 31.145235969507013 35.0 29.5 38.0 8.5 40.0 66-67 30.521267950825568 34.5 28.5 38.0 7.5 40.0 68-69 29.82841877436639 34.0 28.0 37.0 4.5 39.5 70-71 29.081141328789748 34.0 26.5 36.5 2.0 39.0 72-73 28.519373944803164 33.0 26.0 36.0 2.0 39.0 74-75 27.818358452049416 32.5 26.0 35.5 2.0 38.0 76-77 26.64899624970327 31.0 24.5 34.0 2.0 36.0 78-79 27.157706427164914 32.0 25.5 35.0 2.0 37.0 80-81 26.78953317632353 32.0 25.0 35.0 2.0 36.0 82-83 26.129710254476947 32.0 23.0 35.0 2.0 36.0 84-85 25.360550954181598 31.0 20.5 34.0 2.0 35.5 86-87 24.473735087000357 31.0 17.5 34.0 2.0 35.0 88-89 23.532019984679344 30.0 9.0 34.0 2.0 35.0 90-91 22.701251134232606 29.5 2.0 34.0 2.0 35.0 92-93 21.640373546415347 29.0 2.0 34.0 2.0 35.0 94-95 20.482968683295 28.0 2.0 33.0 2.0 35.0 96-97 19.10528689468902 25.5 2.0 33.0 2.0 34.0 98-99 17.343032190812867 21.0 2.0 32.0 2.0 34.0 100-101 13.015993828633658 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3278562844764714 1101 2 0.31691187449366254 1101 3 0.33276206214124926 1101 4 0.20961399418337834 1101 5 0.13405881388259644 1101 6 0.1942628108175981 1101 7 0.27796896450590936 1101 8 0.2524753036867864 1101 9 0.34467857648679967 1101 10-11 0.379032757648325 1101 12-13 0.3639341093278361 1101 14-15 0.45240927584377033 1101 16-17 0.396993614204888 1101 18-19 0.49299824688677774 1101 20-21 0.5251162701703365 1101 22-23 0.47234490716910926 1101 24-25 0.44212341068888605 1101 26-27 0.5743610691565522 1101 28-29 0.6034314577762458 1101 30-31 0.636139059311752 1101 32-33 0.6153998322431207 1101 34-35 0.4385140920471642 1101 36-37 0.44979385023986396 1101 38-39 0.6277699817429365 1101 40-41 0.7081411877974304 1101 42-43 0.6393999013578551 1101 44-45 0.6638113508750294 1101 46-47 0.7564125013718339 1101 48-49 0.777574518217925 1101 50-51 0.8103503925606859 1101 52-53 1.0592368821900493 1101 54-55 1.0102141942523346 1101 56-57 0.9835203219130122 1101 58-59 1.0035971380598596 1101 60-61 1.0386609735676515 1101 62-63 1.0467105132878807 1101 64-65 1.210784165022197 1101 66-67 1.4069172492866606 1101 68-69 1.4817671387740567 1101 70-71 1.7352970295479757 1101 72-73 1.6754183839134704 1101 74-75 1.5990126505027185 1101 76-77 1.652056650479043 1101 78-79 1.6313744344360366 1101 80-81 1.8031565851101625 1101 82-83 1.9991175691340501 1101 84-85 2.2638385825718252 1101 86-87 2.247272322044541 1101 88-89 2.1678849603877914 1101 90-91 2.514483516437739 1101 92-93 2.8948165178705487 1101 94-95 2.6983521632755902 1101 96-97 2.6622107169155917 1101 98-99 2.8248670356200485 1101 100-101 2.5450645685939985 1102 1 0.28868442320655063 1102 2 0.27713255796611236 1102 3 0.20740073406020798 1102 4 0.17875318222608172 1102 5 0.1851896543907614 1102 6 0.29605539612016685 1102 7 0.2590658287432177 1102 8 0.25416167908608145 1102 9 0.332112260449442 1102 10-11 0.34322541431030373 1102 12-13 0.27284235539080726 1102 14-15 0.49254645554083254 1102 16-17 0.33037728450926096 1102 18-19 0.40698729705561476 1102 20-21 0.33971477754568014 1102 22-23 0.44703417271921353 1102 24-25 0.322781315058279 1102 26-27 0.4138551138916995 1102 28-29 0.3133413061090735 1102 30-31 0.3218253854323052 1102 32-33 0.37386002082433833 1102 34-35 0.47571296754419024 1102 36-37 0.3822172958331791 1102 38-39 0.4736431358927575 1102 40-41 0.5502375883881214 1102 42-43 0.5971440319161943 1102 44-45 0.5808988019250023 1102 46-47 0.5478514216746078 1102 48-49 0.6387987111423072 1102 50-51 0.6653590672776346 1102 52-53 0.7259175467043946 1102 54-55 0.7028626825914017 1102 56-57 0.7707313394076678 1102 58-59 1.0411770945950636 1102 60-61 0.9513723302090398 1102 62-63 1.0730188461098713 1102 64-65 1.0926759924982719 1102 66-67 1.1261742443211205 1102 68-69 1.2232033582227189 1102 70-71 1.5087653787361894 1102 72-73 1.491698646806082 1102 74-75 1.4860796935199794 1102 76-77 1.5811380852580363 1102 78-79 1.5700751743516044 1102 80-81 1.737783122138989 1102 82-83 1.8591748272411728 1102 84-85 1.93119748130567 1102 86-87 1.8012974594149576 1102 88-89 1.5905830212456173 1102 90-91 1.828684950875573 1102 92-93 1.950420114934385 1102 94-95 1.9085255818011255 1102 96-97 2.1699985942029976 1102 98-99 2.0706347498469384 1102 100-101 1.8431135331836668 1103 1 0.17727550935679304 1103 2 0.1831901157335949 1103 3 0.2051981304458792 1103 4 0.06027634093562284 1103 5 0.06656129743212347 1103 6 0.03171227280596156 1103 7 0.12273923689096478 1103 8 0.20877836035872122 1103 9 0.21604762134202815 1103 10-11 0.23475718883952368 1103 12-13 0.20805616525398563 1103 14-15 0.2668062963868678 1103 16-17 0.15221554579592578 1103 18-19 0.16889139320725377 1103 20-21 0.14724310574703736 1103 22-23 0.16086205182948987 1103 24-25 0.15224866871721332 1103 26-27 0.23214577163792427 1103 28-29 0.22308076281230882 1103 30-31 0.25591470579416864 1103 32-33 0.2725911197278137 1103 34-35 0.40743151667652455 1103 36-37 0.39880900032192557 1103 38-39 0.21629348933459625 1103 40-41 0.34904268331558086 1103 42-43 0.18023014351037858 1103 44-45 0.33926029580356243 1103 46-47 0.3649377401394034 1103 48-49 0.2852344556548374 1103 50-51 0.352236537363801 1103 52-53 0.46441963302854106 1103 54-55 0.4226797498709516 1103 56-57 0.6138881280029622 1103 58-59 0.6992828986795132 1103 60-61 0.6291696583956181 1103 62-63 0.7398735149634454 1103 64-65 1.0245349181123657 1103 66-67 0.8791575571378907 1103 68-69 0.8572275484436496 1103 70-71 1.2267947202625074 1103 72-73 1.2288645990871707 1103 74-75 1.0587382763199393 1103 76-77 1.140677207103689 1103 78-79 1.2084982633843957 1103 80-81 1.3392999837982522 1103 82-83 1.3870571201403976 1103 84-85 1.5940886457712118 1103 86-87 1.4271825416573876 1103 88-89 1.4686188757860315 1103 90-91 1.689733318814266 1103 92-93 1.787654436813682 1103 94-95 1.6983507875066621 1103 96-97 1.7139314885614532 1103 98-99 1.8355512570982064 1103 100-101 1.8151307602764426 1104 1 0.1589156133792251 1104 2 0.15251368343571414 1104 3 0.1734477333590192 1104 4 0.12916510250207125 1104 5 0.1737715672602107 1104 6 0.07107878000127243 1104 7 0.10143500946761463 1104 8 0.14314141393282398 1104 9 0.1594352895270177 1104 10-11 0.2698811531510401 1104 12-13 0.206362299330884 1104 14-15 0.29825096046252497 1104 16-17 0.31708903503558616 1104 18-19 0.3061034455495104 1104 20-21 0.29992929094389353 1104 22-23 0.3333031094970025 1104 24-25 0.25340332410311106 1104 26-27 0.3667854332148721 1104 28-29 0.26350754763419815 1104 30-31 0.32890961607959923 1104 32-33 0.5683816806786552 1104 34-35 0.43524207998283515 1104 36-37 0.459564881822935 1104 38-39 0.3793257369258143 1104 40-41 0.4877162381263034 1104 42-43 0.515055536697993 1104 44-45 0.5152477609092614 1104 46-47 0.5242125433466569 1104 48-49 0.5959515038113707 1104 50-51 0.592183634889615 1104 52-53 0.6352725023989052 1104 54-55 0.832006277819815 1104 56-57 0.8757154278441348 1104 58-59 0.7892834716304904 1104 60-61 0.9059543211343808 1104 62-63 0.834655204723223 1104 64-65 0.9707516909341223 1104 66-67 1.073277011051939 1104 68-69 1.079029288304806 1104 70-71 1.1384510897267681 1104 72-73 1.4738899521682178 1104 74-75 1.390049888444853 1104 76-77 1.5441410141083303 1104 78-79 1.5006322829856842 1104 80-81 1.6281530632506396 1104 82-83 1.6868177539515088 1104 84-85 1.9808931071495373 1104 86-87 1.9433486719782742 1104 88-89 1.8665206019600227 1104 90-91 2.1335643873996837 1104 92-93 2.4246828265349407 1104 94-95 2.123197284388855 1104 96-97 2.2507766931208693 1104 98-99 2.5520326696133644 1104 100-101 2.4842162350140775 1105 1 0.1268493637538768 1105 2 0.1422980381027159 1105 3 0.0717582281895659 1105 4 0.15670851954679677 1105 5 0.13355349885571854 1105 6 0.09319026096370209 1105 7 0.15768218765330744 1105 8 0.1138964265490543 1105 9 0.11343236377357613 1105 10-11 0.16222907530199393 1105 12-13 0.24938893243992055 1105 14-15 0.27875211066866257 1105 16-17 0.20299152678419574 1105 18-19 0.2732542145986798 1105 20-21 0.2213577849397126 1105 22-23 0.3374358817698422 1105 24-25 0.24148796538313633 1105 26-27 0.2865446276871424 1105 28-29 0.27099317231588316 1105 30-31 0.4538085125682727 1105 32-33 0.4887092530440782 1105 34-35 0.3979319483118857 1105 36-37 0.38599861443106676 1105 38-39 0.39441964550074005 1105 40-41 0.570271910050856 1105 42-43 0.5393374934878281 1105 44-45 0.4685115398448403 1105 46-47 0.4237312113358698 1105 48-49 0.5584950744310575 1105 50-51 0.592089984280463 1105 52-53 0.5341350138693457 1105 54-55 0.5775075354280474 1105 56-57 0.7123503717471138 1105 58-59 0.8797508132640957 1105 60-61 0.8842370158870025 1105 62-63 0.8922732150302117 1105 64-65 1.0467232131188808 1105 66-67 1.14434833788534 1105 68-69 1.2911640785324572 1105 70-71 1.3542780025228929 1105 72-73 1.388343483298062 1105 74-75 1.2468221223340912 1105 76-77 1.2413259350152224 1105 78-79 1.3812376935216513 1105 80-81 1.465668403180267 1105 82-83 1.6965037162571868 1105 84-85 1.7326538992028269 1105 86-87 1.848668408006013 1105 88-89 1.9288800633114285 1105 90-91 1.9288029013482024 1105 92-93 1.924567143440573 1105 94-95 1.8243527201091005 1105 96-97 2.1940043488182717 1105 98-99 2.25386805091388 1105 100-101 2.283192241386736 1106 1 0.023266305925822905 1106 2 0.12705201092493468 1106 3 0.08228447419229923 1106 4 0.08675793485815575 1106 5 0.02548161228799728 1106 6 0.05379674166319148 1106 7 0.012900540119382242 1106 8 -0.05579345103527089 1106 9 -0.11822707895026241 1106 10-11 0.14589418754200523 1106 12-13 0.11324984366107316 1106 14-15 0.10725557945747966 1106 16-17 -0.035418030253190125 1106 18-19 0.07136561936439278 1106 20-21 0.014003909109597146 1106 22-23 0.19905989892325948 1106 24-25 0.14036642117221731 1106 26-27 -0.14273855789699041 1106 28-29 0.06417833392865901 1106 30-31 0.1658456565277362 1106 32-33 0.014030407050263705 1106 34-35 -0.016088536793120056 1106 36-37 -0.045411084997297735 1106 38-39 0.04442953568750596 1106 40-41 0.06638109375471402 1106 42-43 0.15991544453600426 1106 44-45 0.13732139365709628 1106 46-47 -0.2279783165999234 1106 48-49 0.01889915015772914 1106 50-51 0.19551541261856187 1106 52-53 -0.009239664303777317 1106 54-55 -0.2369352938006415 1106 56-57 -0.09760580567145638 1106 58-59 0.18509838134188072 1106 60-61 0.2755843838851675 1106 62-63 0.10170798813527782 1106 64-65 0.2380018000382833 1106 66-67 0.23169367817257935 1106 68-69 0.2669015059974136 1106 70-71 0.09128086726323303 1106 72-73 0.4350691487136835 1106 74-75 0.4344946254478437 1106 76-77 0.450152850018096 1106 78-79 0.5948029430764414 1106 80-81 0.7297953154026082 1106 82-83 1.0308790072359812 1106 84-85 1.1651152535083042 1106 86-87 1.2292642177926396 1106 88-89 1.175048098326748 1106 90-91 1.4905347358853298 1106 92-93 1.3390342454337762 1106 94-95 1.104716310883159 1106 96-97 1.2642801865725026 1106 98-99 1.542208604231977 1106 100-101 1.5581709990071033 1107 1 0.02644249188068315 1107 2 -0.009045367518012881 1107 3 0.07174561696847803 1107 4 0.019177694993167904 1107 5 0.008709186262322532 1107 6 0.028342797447301393 1107 7 0.0714509870782365 1107 8 0.025277846531139403 1107 9 0.12708034017139624 1107 10-11 0.05496856944897388 1107 12-13 0.14352822507088092 1107 14-15 0.11530127400826018 1107 16-17 0.010917735706200915 1107 18-19 0.04459202422533082 1107 20-21 0.14033299523182308 1107 22-23 0.15982763184781135 1107 24-25 0.19103230502477686 1107 26-27 0.2365066904989277 1107 28-29 0.1312595250698081 1107 30-31 0.12425208309483082 1107 32-33 0.23953171170892062 1107 34-35 0.19091273088144334 1107 36-37 0.22657155726327716 1107 38-39 0.17290157070339518 1107 40-41 0.20774104314610753 1107 42-43 0.32293369235285496 1107 44-45 0.26192750365552797 1107 46-47 0.34335524071888557 1107 48-49 0.36243976086245056 1107 50-51 0.48389751860039354 1107 52-53 0.49040080495336724 1107 54-55 0.6130023818619605 1107 56-57 0.47269910212654764 1107 58-59 0.5740688370142237 1107 60-61 0.6929879892568067 1107 62-63 0.9801125815779876 1107 64-65 0.966170073668323 1107 66-67 0.7420370210461051 1107 68-69 1.063522408874551 1107 70-71 1.0237009111636297 1107 72-73 1.2582433502026227 1107 74-75 1.1467340602477307 1107 76-77 1.269108784469804 1107 78-79 1.3840652892826988 1107 80-81 1.4599165570010264 1107 82-83 1.5647607833878467 1107 84-85 1.7935841198107276 1107 86-87 2.050728827425111 1107 88-89 2.090331597918219 1107 90-91 2.237685022005966 1107 92-93 2.28098114460645 1107 94-95 2.223950742142602 1107 96-97 2.241691657920615 1107 98-99 2.281552504609902 1107 100-101 2.056873008841656 1108 1 0.056417571631090624 1108 2 0.04471117089393317 1108 3 0.07466495132590012 1108 4 0.007773667387475314 1108 5 0.02080051799597271 1108 6 0.034547105651085985 1108 7 0.06596411716250117 1108 8 0.06732305898397328 1108 9 0.025438168605710132 1108 10-11 0.08213468996319051 1108 12-13 0.10852117858298271 1108 14-15 0.2834300022345744 1108 16-17 0.1415998644673664 1108 18-19 0.0652779863319708 1108 20-21 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50-51 -0.9179166637600744 2316 52-53 -0.7094173781937911 2316 54-55 -0.4939080864269272 2316 56-57 -0.6595344539958958 2316 58-59 -0.5614673314675755 2316 60-61 -0.3057986943245865 2316 62-63 -0.4315125884290971 2316 64-65 -0.49571417175518917 2316 66-67 -0.5663425328532625 2316 68-69 -0.10146975562822647 2316 70-71 -0.2955934334245214 2316 72-73 -0.13928727402107555 2316 74-75 0.16628493896402574 2316 76-77 0.15764375812115006 2316 78-79 0.07979484555336214 2316 80-81 -0.5091098984049758 2316 82-83 -0.06075726970314932 2316 84-85 0.22536510563680068 2316 86-87 0.3206805752449995 2316 88-89 0.11909324428546242 2316 90-91 0.2220505745117869 2316 92-93 0.3516691605878428 2316 94-95 0.5052597112923785 2316 96-97 0.43980909025733084 2316 98-99 0.3866581482587286 2316 100-101 0.16390361462188707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 17.0 4 61.0 5 129.0 6 251.0 7 684.0 8 1528.0 9 2823.0 10 4650.0 11 7149.0 12 10401.0 13 14335.0 14 19218.0 15 26511.0 16 33879.0 17 43219.0 18 41800.0 19 35476.0 20 35800.0 21 39198.0 22 42965.0 23 48314.0 24 54719.0 25 62704.0 26 72957.0 27 85331.0 28 100573.0 29 118058.0 30 137390.0 31 161643.0 32 191021.0 33 229938.0 34 282409.0 35 345048.0 36 403518.0 37 372317.0 38 126852.0 39 2324.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.25254284840233 19.726099919636955 15.716221763960899 22.305135467999815 2 15.698188946028463 21.26945577092779 39.450648652486585 23.58170663055716 3 18.83061212523632 25.4397010606452 30.05693856561722 25.672748248501264 4 13.079353971533495 17.827204911738242 36.726760790145335 32.36668032658292 5 13.74756197202472 37.83762369208554 33.633788389630624 14.781025946259113 6 31.398125707360226 36.62898621552812 17.52408788365428 14.448800193457375 7 28.44523847329676 33.1294170892208 20.44055923887327 17.98478519860916 8 26.609620387320547 34.908386620757504 19.664612501965 18.817380489956946 9 26.7961839539326 16.55522639906212 19.94828858872155 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22.358743541792133 26.739674791594027 29.230514437858567 21.67106722875527 36-37 22.348407834787473 26.459356021811086 29.196565208785728 21.99567093461571 38-39 21.926364385146087 26.69725691034207 29.317788012210528 22.058590692301316 40-41 22.33674002261617 26.363638524829845 29.34138132969446 21.958240122859525 42-43 22.195482115876683 26.428997727777688 29.083669063767704 22.291851092577925 44-45 22.089386579715857 26.785373577662735 28.939266492012855 22.185973350608553 46-47 22.254486262507267 26.99157949750183 28.342739188496 22.411195051494897 48-49 22.23404919534853 26.93979526606252 28.348890108033775 22.477265430555175 50-51 22.478485631891562 27.068201648254302 28.02445917348949 22.428853546364643 52-53 22.680421029678783 27.104062890127683 27.87629186835644 22.339224211837095 54-55 22.246869232765924 27.148560621979406 27.95742733384106 22.647142811413605 56-57 22.273127331654212 27.33972021575695 27.83342635387677 22.553726098712072 58-59 22.311064500476512 26.961584575640913 27.944369594864632 22.78298132901794 60-61 22.22538101182898 27.11751679836918 28.043316830506427 22.613785359295417 62-63 22.147129658594007 27.207732463409013 28.038293404225445 22.606844473771535 64-65 21.97144695821886 26.974464977114877 28.038845524180516 23.015242540485744 66-67 21.98849484920982 27.106624613276736 27.95514572710312 22.949734810410323 68-69 22.242170018826187 26.876897673032918 27.963190689596157 22.917741618544742 70-71 22.079039584129966 26.921433125112017 27.83290782106394 23.166619469694076 72-73 22.213372667193205 26.982703539413517 27.64424387331184 23.159679920081437 74-75 22.12763813075297 27.21194770438927 27.38133383535903 23.279080329498733 76-77 22.017677389542463 26.942806152476994 27.875610457220535 23.16390600076001 78-79 22.08952664745404 27.076885678544453 27.737775246520286 23.095812427481217 80-81 21.958697261311393 27.420227689749776 27.5386190067268 23.082456042212026 82-83 22.186941542200454 27.26872230639615 27.425718427211688 23.11861772419171 84-85 22.119343813777352 26.989536354614664 27.496078470525852 23.395041361082136 86-87 22.07618543516536 26.975941858567097 27.451985933728785 23.49588677253876 88-89 22.0436343997893 27.02143782820643 27.27433644025118 23.660591331753096 90-91 21.97474077016928 26.983958200581366 27.548176188016882 23.493124841232476 92-93 21.918418865350166 26.70682785019345 27.532923798076354 23.841829486380036 94-95 21.821180892024366 26.763076321387135 27.480047724141908 23.935695062446584 96-97 21.928919627398052 26.687150202347414 27.424738140039178 23.959192030215355 98-99 21.883043226503915 26.613716267375587 27.215560768086632 24.287679738033866 100-101 21.95260166023499 26.45448045395307 27.38449602182545 24.20842186398649 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1842.0 1 1221.0 2 764.5 3 909.0 4 779.0 5 757.0 6 1293.5 7 2900.0 8 4427.0 9 4764.0 10 4079.5 11 3290.5 12 3283.0 13 3540.5 14 3827.5 15 4099.0 16 4316.5 17 4586.5 18 4921.0 19 5279.0 20 5849.0 21 6548.0 22 7512.5 23 8919.0 24 10451.0 25 12543.0 26 15177.0 27 18941.0 28 23062.5 29 27685.5 30 33734.0 31 39835.0 32 47277.5 33 54051.0 34 59442.5 35 65587.5 36 72098.5 37 77886.0 38 83412.0 39 87889.5 40 91945.0 41 99086.5 42 107225.5 43 113676.5 44 116963.5 45 119688.0 46 120858.0 47 122214.5 48 126047.5 49 125854.5 50 122457.0 51 118131.5 52 111986.0 53 107635.5 54 104853.0 55 96387.5 56 85150.5 57 76329.5 58 68423.5 59 60264.0 60 50363.5 61 41334.5 62 34720.0 63 27177.5 64 20646.5 65 16029.5 66 11985.5 67 9580.0 68 7261.5 69 5056.5 70 3928.5 71 3181.5 72 2716.5 73 2332.5 74 1866.0 75 1502.5 76 1047.5 77 598.5 78 371.0 79 225.0 80 108.5 81 61.5 82 35.5 83 14.0 84 8.0 85 8.0 86 7.5 87 5.0 88 3.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024784349222256346 2 0.011694916704619682 3 0.001964999554705746 4 0.0 5 9.508062361479417E-5 6 0.0 7 0.0 8 3.169354120493139E-5 9 4.437095768690395E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001663910913258898 26-27 0.008747417372561064 28-29 1.5846770602465695E-4 30-31 0.0 32-33 3.169354120493139E-4 34-35 0.010046852561963249 36-37 0.0010300400891602702 38-39 0.012629876170165158 40-41 0.012503102005345431 42-43 0.004944192427969296 44-45 7.289514477134219E-4 46-47 4.7540311807397084E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.7540311807397084E-5 66-67 6.814111359060249E-4 68-69 0.0011726610245824615 70-71 1.5846770602465696E-5 72-73 1.5846770602465695E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.923385301232847E-5 82-83 0.0013786690424145154 84-85 0.006275321158576415 86-87 0.004817418263149571 88-89 0.0 90-91 0.0016956044544638295 92-93 4.5955634747150514E-4 94-95 1.5846770602465696E-5 96-97 0.0 98-99 0.0 100-101 3.169354120493139E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3155217.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.621207125823304 #Duplication Level Percentage of deduplicated Percentage of total 1 72.02277361458425 35.01834193690983 2 14.72714752331728 14.3210338020753 3 5.436987491508992 7.930586848955075 4 2.5742137321939023 5.006455162365534 5 1.451612654093404 3.528957976057074 6 0.8680215178785797 2.5322552406267573 7 0.5843348966868868 1.9887747629861778 8 0.3974081664156624 1.545797181823166 9 0.2830334537134572 1.2385285358885212 >10 1.4690304551900881 12.804787168401678 >50 0.10718118993323922 3.6161839966645437 >100 0.07160567116545066 6.517156518080178 >500 0.004628666330157717 1.5216323641008203 >1k 0.0018253895381451226 1.3219389174234946 >5k 6.519248350518295E-5 0.27564035069930964 >10k+ 1.303849670103659E-4 0.8319292369427417 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14450 0.4579716704112585 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11796 0.37385701205337063 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8696 0.2756070343180833 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.169354120493139E-5 0.0 0.0 0.0 0.0 3 3.169354120493139E-5 0.0 0.0 0.0 0.0 4 3.169354120493139E-5 0.0 0.0 3.169354120493139E-5 0.0 5 6.338708240986278E-5 0.0 0.0 3.169354120493139E-5 0.0 6 6.338708240986278E-5 0.0 0.0 3.169354120493139E-5 0.0 7 6.338708240986278E-5 0.0 0.0 3.169354120493139E-5 0.0 8 6.338708240986278E-5 0.0 0.0 6.338708240986278E-5 0.0 9 6.338708240986278E-5 0.0 0.0 6.338708240986278E-5 0.0 10-11 9.508062361479417E-5 0.0 0.0 7.923385301232847E-5 0.0 12-13 1.2677416481972557E-4 0.0 0.0 1.1092739421725986E-4 0.0 14-15 2.2185478843451972E-4 0.0 0.0 1.5846770602465695E-4 0.0 16-17 2.2185478843451972E-4 0.0 0.0 2.2185478843451972E-4 0.0 18-19 2.6939510024191684E-4 0.0 0.0 2.2185478843451972E-4 0.0 20-21 3.169354120493139E-4 0.0 0.0 3.3278218265177955E-4 0.0 22-23 3.8032249445917667E-4 0.0 0.0 0.0010934271715701328 0.0 24-25 5.070966592789023E-4 0.0 0.0 0.001964999554705746 0.0 26-27 5.070966592789023E-4 0.0 0.0 0.0032802815147103988 0.0 28-29 6.814111359060249E-4 0.0 0.0 0.007210280624121891 0.0 30-31 7.606449889183533E-4 0.0 0.0 0.019713382629467324 0.0 32-33 7.606449889183533E-4 0.0 0.0 0.04310321603870669 0.0 34-35 8.715723831356132E-4 0.0 0.0 0.07582679733279835 0.0 36-37 9.82499777352873E-4 0.0 0.0 0.12791513230310309 0.0 38-39 0.0010775804009676671 0.0 0.0 0.20779870291013264 0.0 40-41 0.0010775804009676671 0.0 0.0 0.2956215055889975 0.0 42-43 0.0012043545657873928 0.0 0.0 0.3883885006958317 0.0 44-45 0.0014262093542219125 0.0 0.0 0.5006311768730962 0.0 46-47 0.0014420561248243782 0.0 0.0 0.6311768730962086 0.0 48-49 0.0014579028954268439 0.0 0.0 0.7738928891420147 0.0 50-51 0.0014579028954268439 0.0 0.0 0.9304431359237733 0.0 52-53 0.0014737496660293095 0.0 0.0 1.0843152784737151 0.0 54-55 0.001552983519041638 0.0 0.0 1.2516888695769577 0.0 56-57 0.0016005238308490352 0.0 0.0 1.444496527497158 0.0 58-59 0.0016163706014515008 0.0 0.0 1.6544662379798285 0.0 60-61 0.0016163706014515008 0.0 0.0 1.8879842495777628 0.0 62-63 0.001648064142656432 0.0 0.0 2.1218508901289512 0.0 64-65 0.0016797576838613636 0.0 0.0 2.368727729344765 0.0 66-67 0.0016797576838613636 0.0 0.0 2.6468068598768326 0.0 68-69 0.001711451225066295 0.0 0.0 2.955470257671659 0.0 70-71 0.0017748383074761578 0.0 0.0 3.2983468331972094 0.0 72-73 0.0018857657016934175 0.0 0.0 3.6510008661844813 0.0 74-75 0.001964999554705746 0.0 0.0 4.008916027011772 0.0 76-77 0.001964999554705746 0.0 0.0 4.389729771359624 0.0 78-79 0.001964999554705746 0.0 0.0 4.804598225732176 0.0 80-81 0.0019966930959106773 0.0 0.0 5.255882558949194 0.0 82-83 0.002028386637115609 0.0 0.0 5.727403218225561 0.0 84-85 0.002028386637115609 0.0 0.0 6.2008888770566335 0.0 86-87 0.002028386637115609 0.0 1.5846770602465696E-5 6.68532465437401 0.0 88-89 0.002028386637115609 0.0 3.169354120493139E-5 7.207824374678509 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7470 0.0 56.481297 1 GTATCAA 13770 0.0 43.061874 1 TATCAAC 18220 0.0 32.485203 2 ATCAACG 18335 0.0 32.019833 3 TCAACGC 18770 0.0 31.276773 4 CAACGCA 19220 0.0 30.569199 5 AACGCAG 19755 0.0 29.741333 6 ACGCAGA 22540 0.0 25.91904 7 GTACATG 16850 0.0 25.716227 1 CGCAGAG 22770 0.0 25.552938 8 TACATGG 17145 0.0 24.991076 2 ACATGGG 17525 0.0 23.850977 3 GCAGAGT 24650 0.0 23.218699 9 GAGTACT 13075 0.0 22.849499 12-13 TGGTATC 3420 0.0 22.084467 2 AGTACTT 13390 0.0 21.957243 12-13 GTACTTT 13880 0.0 21.438745 14-15 GTGGTAT 3875 0.0 21.334862 1 AGAGTAC 22810 0.0 20.7709 10-11 GTGTAGC 1670 0.0 20.76915 1 >>END_MODULE