FastQCFastQC Report
Thu 26 May 2016
SRR938575_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938575_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3688416
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95470.2588373979507735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73400.19900141415718836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50070.13574932979360246No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55300.055.0469931
GTATCAA106000.047.025581
ATCAACG138400.035.63773
TATCAAC140050.035.4555632
TCAACGC140850.035.01784
CAACGCA145150.033.914885
AACGCAG151600.032.5695536
ACGCAGA171700.028.5365567
CGCAGAG173450.028.2756548
GCAGAGT192500.024.6124029
GTACATG140250.023.9091991
TGGTATC24800.023.7813762
TACATGG138150.023.7211272
GAGTACT104550.022.04306212-13
ACATGGG146000.021.7941653
GTGGTAT31050.021.1395761
AGAGTAC177500.020.92267610-11
CAGAGTA181950.020.8157910-11
AGTACTT109550.020.68998512-13
GTACTTT112750.020.11893514-15
Produced by FastQC (version 0.11.5)