FastQCFastQC Report
Thu 26 May 2016
SRR938575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938575_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3688416
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95540.2590271813157735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78790.21361473326219166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56080.15204358727432046No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60750.051.4116441
GTATCAA104650.042.8820151
TATCAAC136250.032.9360852
ATCAACG136200.032.8922923
TCAACGC138050.032.4515084
CAACGCA141950.031.559925
AACGCAG149700.029.9732636
TGGTATC26550.025.783062
ACGCAGA174300.025.6884757
CGCAGAG175400.025.4461638
GTACATG136850.023.6561871
TACATGG134150.023.5295662
GCAGAGT196050.022.4510619
GAGTACT106700.022.16097812-13
GTGGTAT33900.022.0162011
ACATGGG140650.021.5158883
GTAAGGT19800.021.3542544
TAAGGTG23150.021.137125
GTACTTT113150.020.62495414-15
AGTACTT112000.019.9464512-13
Produced by FastQC (version 0.11.5)