FastQCFastQC Report
Thu 26 May 2016
SRR938574_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938574_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3672579
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98510.2682311258655022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74000.20149328305803632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52580.1431691462593453No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58200.050.6362461
GTATCAA104500.043.60031
ATCAACG135600.033.436313
TATCAAC136650.033.07462
TCAACGC137100.033.0358244
CAACGCA139200.032.398665
AACGCAG146100.030.8964356
ACGCAGA168000.026.5262177
CGCAGAG169650.026.2682258
TGGTATC28650.025.71172
GTACATG137450.025.4240741
TACATGG136800.025.047992
GTGGTAT33900.024.2963051
GCAGAGT188800.022.9983339
ACATGGG144350.022.8821953
GAGTACT103200.021.17129512-13
TAAGGTG19500.020.4712685
AGTACTT107450.020.46651512-13
AGAGTAC174200.020.43780310-11
CAGAGTA178200.019.7657910-11
Produced by FastQC (version 0.11.5)