##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938574_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3672579 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.956424354656495 31.0 31.0 34.0 28.0 34.0 2 31.05292711198316 31.0 31.0 34.0 27.0 34.0 3 31.115583354367597 31.0 31.0 34.0 27.0 34.0 4 34.74637359740934 37.0 35.0 37.0 32.0 37.0 5 34.75998365181525 37.0 35.0 37.0 32.0 37.0 6 34.787326834902665 37.0 35.0 37.0 32.0 37.0 7 34.70791942120238 37.0 35.0 37.0 31.0 37.0 8 34.66419510649056 37.0 35.0 37.0 30.0 37.0 9 36.153249800753095 39.0 35.0 39.0 31.0 39.0 10-11 35.996637921199245 39.0 35.0 39.0 30.0 39.0 12-13 35.83562545557223 38.0 35.0 39.0 30.0 39.0 14-15 36.79762096336117 39.0 36.0 41.0 30.5 41.0 16-17 36.64188598802095 39.0 36.0 41.0 30.0 41.0 18-19 36.63099922425086 39.0 36.0 40.5 30.0 41.0 20-21 36.72242462313268 39.0 36.0 41.0 30.0 41.0 22-23 36.63618931001892 39.0 36.0 41.0 30.0 41.0 24-25 36.51276732236393 39.0 36.0 41.0 29.5 41.0 26-27 36.32195427246086 39.0 35.5 41.0 29.0 41.0 28-29 36.134585804689294 39.0 35.0 40.5 28.5 41.0 30-31 35.91665911066855 38.5 35.0 40.0 27.0 41.0 32-33 35.630618156886484 38.0 34.5 40.0 27.0 41.0 34-35 35.3775627426939 38.0 34.0 40.0 26.5 41.0 36-37 35.0815190088491 38.0 34.0 40.0 25.0 41.0 38-39 34.819445953374995 38.0 33.5 40.0 25.0 41.0 40-41 34.75091373119544 38.0 33.5 40.0 24.5 41.0 42-43 34.56897319295242 38.0 33.0 40.0 24.0 41.0 44-45 34.26975076642327 38.0 33.0 40.0 23.5 41.0 46-47 33.901528326552 38.0 33.0 40.0 22.5 41.0 48-49 33.540281230165505 38.0 32.5 40.0 19.5 41.0 50-51 32.804535995005146 36.5 31.5 39.5 18.5 40.5 52-53 32.86380687794599 37.0 31.5 39.0 18.0 40.0 54-55 32.98073901201308 37.0 32.0 40.0 17.5 41.0 56-57 32.66856887217402 37.0 31.0 40.0 14.0 41.0 58-59 32.32689943497471 36.5 31.0 39.5 11.5 41.0 60-61 31.86359912203386 36.0 30.5 39.0 8.0 41.0 62-63 31.330285611282967 35.5 30.0 39.0 7.0 40.0 64-65 30.82318229233462 35.0 29.0 38.5 3.5 40.0 66-67 30.272479366679384 35.0 28.5 38.0 2.0 40.0 68-69 29.697083847617712 34.0 28.0 37.5 2.0 40.0 70-71 29.06256257523664 34.0 26.5 37.0 2.0 39.0 72-73 28.49545809634047 34.0 26.0 36.0 2.0 39.0 74-75 27.86303671071473 33.0 26.0 36.0 2.0 38.5 76-77 27.111232460894648 32.5 24.5 35.0 2.0 37.5 78-79 26.32445823493518 32.0 22.5 35.0 2.0 37.0 80-81 25.661481890518896 31.5 20.5 35.0 2.0 36.0 82-83 24.990406469132452 31.0 18.5 34.5 2.0 36.0 84-85 24.31806477137728 31.0 14.0 34.0 2.0 35.5 86-87 23.63246086741769 30.5 7.0 34.0 2.0 35.0 88-89 22.831141821591856 29.5 2.0 34.0 2.0 35.0 90-91 21.99231766015108 29.0 2.0 34.0 2.0 35.0 92-93 21.1204728339404 29.0 2.0 34.0 2.0 35.0 94-95 20.149727752622884 27.5 2.0 34.0 2.0 35.0 96-97 18.90316518718862 25.5 2.0 33.0 2.0 35.0 98-99 17.42380477043516 21.5 2.0 32.5 2.0 34.0 100-101 14.074340402207822 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7016202311351485 1101 2 0.6451437649255816 1101 3 0.7159375351305606 1101 4 0.5955525119588003 1101 5 0.6986160309084042 1101 6 0.6181434036638223 1101 7 0.5665736322734034 1101 8 0.6746843146560835 1101 9 0.7268922391156067 1101 10-11 0.810699297682568 1101 12-13 0.8123456363202521 1101 14-15 0.8775398331450788 1101 16-17 0.9867248231375072 1101 18-19 0.8668733355483127 1101 20-21 0.9076404198845793 1101 22-23 0.9978619431331666 1101 24-25 1.055616891435072 1101 26-27 1.0851214628001102 1101 28-29 1.0565687356090763 1101 30-31 1.1053799342702888 1101 32-33 1.2564233758865058 1101 34-35 1.0597824673242329 1101 36-37 1.064593957676827 1101 38-39 1.018095237211078 1101 40-41 1.0202273325040068 1101 42-43 1.0960505305321604 1101 44-45 1.028933470180796 1101 46-47 1.1739721142270767 1101 48-49 1.170748891920823 1101 50-51 1.0154757134940482 1101 52-53 1.2380669704773553 1101 54-55 1.154911056134253 1101 56-57 1.1334732470236446 1101 58-59 1.16297330644921 1101 60-61 1.1234584720369796 1101 62-63 1.217182432660195 1101 64-65 1.2018795661206276 1101 66-67 1.0647246399911765 1101 68-69 1.1680834519408627 1101 70-71 1.1710611200096714 1101 72-73 1.2123424967882457 1101 74-75 1.1365830688109781 1101 76-77 1.3416256958230903 1101 78-79 1.2082781212830476 1101 80-81 1.361310906635115 1101 82-83 1.2784522274031822 1101 84-85 1.3561858796070254 1101 86-87 1.606108901532476 1101 88-89 1.674054254548885 1101 90-91 1.7289260440172676 1101 92-93 1.8500565991964457 1101 94-95 1.7483498500322519 1101 96-97 1.70857573793349 1101 98-99 1.6631799403546133 1101 100-101 1.2724724055541454 1102 1 0.5232890936735757 1102 2 0.6374920929260846 1102 3 0.5452254452880361 1102 4 0.5216733476448567 1102 5 0.5139519992878547 1102 6 0.4628061805005146 1102 7 0.38933917590851763 1102 8 0.4809732913798399 1102 9 0.585065273810379 1102 10-11 0.6768527577490744 1102 12-13 0.678312760983026 1102 14-15 0.7304339921261942 1102 16-17 0.8098197475367783 1102 18-19 0.865986830748767 1102 20-21 0.6666441214969794 1102 22-23 0.7539733679402474 1102 24-25 0.6262831816665866 1102 26-27 0.6436803435328997 1102 28-29 0.5112961338147315 1102 30-31 0.5332938560972593 1102 32-33 0.5104531142640738 1102 34-35 0.7037933839438466 1102 36-37 0.6564403721319323 1102 38-39 0.7704435652115862 1102 40-41 0.7368049095163656 1102 42-43 0.889115340118714 1102 44-45 0.9264916607824674 1102 46-47 0.8185294892192374 1102 48-49 0.6522282820958623 1102 50-51 0.7329837127411807 1102 52-53 0.6890984028131371 1102 54-55 0.7020470119658668 1102 56-57 0.8225108278003503 1102 58-59 0.936423962073782 1102 60-61 0.8794318706190722 1102 62-63 0.6521488671918476 1102 64-65 0.7519642640314146 1102 66-67 0.8725996635183755 1102 68-69 0.7337080206089084 1102 70-71 0.6678708320367726 1102 72-73 0.8149982468353016 1102 74-75 0.9112728235007346 1102 76-77 0.77611907683821 1102 78-79 0.7119383258128202 1102 80-81 0.8743167413800812 1102 82-83 0.9169320567525752 1102 84-85 0.8464286802846068 1102 86-87 0.8518388720450538 1102 88-89 1.027767787392854 1102 90-91 1.0715805392804576 1102 92-93 1.0711482611576706 1102 94-95 1.0270561678690022 1102 96-97 0.9371779087095753 1102 98-99 1.1031241024726839 1102 100-101 0.7904365187356515 1103 1 0.6573637855464334 1103 2 0.7010159289269815 1103 3 0.635684425655274 1103 4 0.6030824584117624 1103 5 0.650454640377653 1103 6 0.6723035129336239 1103 7 0.6167959189615857 1103 8 0.4839572595014232 1103 9 0.5515744017873985 1103 10-11 0.6981535772150309 1103 12-13 0.6615938020622352 1103 14-15 0.8302301185278296 1103 16-17 0.9458947836118199 1103 18-19 0.8739061285763228 1103 20-21 0.8172564042193571 1103 22-23 0.8971323496313417 1103 24-25 1.0056628234315497 1103 26-27 0.9263179913081601 1103 28-29 0.9282335287337844 1103 30-31 1.0408186452734256 1103 32-33 1.003653797373012 1103 34-35 1.1578582792931442 1103 36-37 1.257095680126696 1103 38-39 0.9997166366864931 1103 40-41 1.2033590485475685 1103 42-43 1.064969557257335 1103 44-45 1.1245085266892403 1103 46-47 1.0872706359420405 1103 48-49 1.145421022077599 1103 50-51 1.1543745543155026 1103 52-53 1.1210456604484662 1103 54-55 1.240036341698307 1103 56-57 1.3052967472835277 1103 58-59 1.3242365572258237 1103 60-61 1.3478089573447818 1103 62-63 1.3880901939588597 1103 64-65 1.3497878471760991 1103 66-67 1.409848958565835 1103 68-69 1.4755656532922288 1103 70-71 1.6371905031913094 1103 72-73 1.5234854782583156 1103 74-75 1.3241115647798445 1103 76-77 1.3625019090926038 1103 78-79 1.3717447782798224 1103 80-81 1.5033290135116175 1103 82-83 1.6215880531516937 1103 84-85 1.6180915739658523 1103 86-87 1.7101824652510338 1103 88-89 1.6100704999707958 1103 90-91 1.7401350225138366 1103 92-93 1.8561476654231335 1103 94-95 1.6295092703221066 1103 96-97 1.6023883353878894 1103 98-99 1.6186939556572177 1103 100-101 1.149205069247321 1104 1 0.5839034337961131 1104 2 0.6030173691070964 1104 3 0.5994775683926186 1104 4 0.631378123743076 1104 5 0.6496946719157748 1104 6 0.562294033267051 1104 7 0.45820109722945546 1104 8 0.5465636209045925 1104 9 0.6085791006028956 1104 10-11 0.6185146932634638 1104 12-13 0.6795765700337242 1104 14-15 0.7639867326461527 1104 16-17 0.7753408839595579 1104 18-19 0.8303967377816264 1104 20-21 0.8325774595614632 1104 22-23 0.9083715653712332 1104 24-25 0.79250807698984 1104 26-27 0.8365398519661866 1104 28-29 0.9085703987119444 1104 30-31 0.8136051659167691 1104 32-33 0.9083311924700226 1104 34-35 0.7357304359628714 1104 36-37 0.7987386476269336 1104 38-39 0.8374715736330316 1104 40-41 0.8338006278235568 1104 42-43 0.8544040586861215 1104 44-45 0.9905585592755912 1104 46-47 1.1596219122788725 1104 48-49 1.0232257272736334 1104 50-51 0.7961623373952094 1104 52-53 0.8475027034305427 1104 54-55 1.0550654871154848 1104 56-57 1.2433675211980315 1104 58-59 1.181304263921291 1104 60-61 1.1828217412220567 1104 62-63 1.252079082695829 1104 64-65 1.068029482965489 1104 66-67 0.9684179163038422 1104 68-69 1.0589161913088816 1104 70-71 1.0526838332432185 1104 72-73 1.1233617412643682 1104 74-75 1.0773480961333846 1104 76-77 1.1093234862721282 1104 78-79 1.2264629632882773 1104 80-81 1.2629264922849117 1104 82-83 1.3442053754193388 1104 84-85 1.0056881366752108 1104 86-87 1.1064807238180556 1104 88-89 1.215896734948032 1104 90-91 1.2285625372460593 1104 92-93 1.1953179439339117 1104 94-95 1.2267770298849463 1104 96-97 1.087671722727979 1104 98-99 1.1868660890835265 1104 100-101 0.9325650641428851 1105 1 0.4894077583259637 1105 2 0.5195874495082435 1105 3 0.5066114037414451 1105 4 0.49233767842648746 1105 5 0.3249357369578121 1105 6 0.4818368728449869 1105 7 0.4765650162135344 1105 8 0.44580637292846603 1105 9 0.5707994484008552 1105 10-11 0.5783922413999889 1105 12-13 0.5266511934856766 1105 14-15 0.6746296140344228 1105 16-17 0.6362440238144274 1105 18-19 0.6584117079243796 1105 20-21 0.7589653226158362 1105 22-23 0.7629601778068249 1105 24-25 0.7593219903652155 1105 26-27 0.8569754225171309 1105 28-29 0.8484688665886395 1105 30-31 0.8392952186968614 1105 32-33 0.782916330422303 1105 34-35 0.8251845050417117 1105 36-37 0.8761925651979112 1105 38-39 0.8398489724469016 1105 40-41 0.8212285341159458 1105 42-43 0.8413778055776575 1105 44-45 0.7981910594613382 1105 46-47 0.8125357436360474 1105 48-49 0.7622738572440824 1105 50-51 0.6143778806456126 1105 52-53 0.5892710551852005 1105 54-55 0.6063578200889808 1105 56-57 0.8162259429230971 1105 58-59 0.8941465587993065 1105 60-61 0.9668210536094293 1105 62-63 0.8102557000787414 1105 64-65 0.9692320402434724 1105 66-67 0.9521924070407408 1105 68-69 0.902263284178833 1105 70-71 0.9606308579346283 1105 72-73 0.9748921164065933 1105 74-75 1.0330071335048387 1105 76-77 0.9121204817545703 1105 78-79 1.0988666793100883 1105 80-81 1.084249330784811 1105 82-83 1.115253350968299 1105 84-85 0.996709875140585 1105 86-87 1.1827064078979284 1105 88-89 1.3123611021914456 1105 90-91 1.4275867683072114 1105 92-93 1.2947711680650364 1105 94-95 0.9822596479991681 1105 96-97 1.0047556551729535 1105 98-99 1.116656578468504 1105 100-101 1.12172045148586 1106 1 0.49241715899541205 1106 2 0.4574496056171604 1106 3 0.2838935438177259 1106 4 0.413983344453122 1106 5 0.46853374971735207 1106 6 0.11141378204928998 1106 7 0.3442501626534096 1106 8 0.3867938068889387 1106 9 0.47645938527369225 1106 10-11 0.2046851713946296 1106 12-13 0.4218453913668654 1106 14-15 0.5411797246712311 1106 16-17 0.4786215723939691 1106 18-19 0.035899616924531585 1106 20-21 0.384749507076414 1106 22-23 0.44091595246068493 1106 24-25 0.5812068047562988 1106 26-27 0.37079801632531684 1106 28-29 0.3273351889541374 1106 30-31 0.4388387917460008 1106 32-33 0.5831628727869784 1106 34-35 0.523296487529997 1106 36-37 0.4470548326425927 1106 38-39 0.50328369701365 1106 40-41 0.5883487136553214 1106 42-43 0.24489546925524763 1106 44-45 0.6659313061044614 1106 46-47 0.2720047721062855 1106 48-49 0.7282523733448727 1106 50-51 0.303750431604783 1106 52-53 0.4695538182166459 1106 54-55 0.45129307539877317 1106 56-57 -0.11587158947479992 1106 58-59 -0.3331317033296415 1106 60-61 0.28753411055522804 1106 62-63 0.3655138950897481 1106 64-65 0.4881868649598964 1106 66-67 -0.05896277634856517 1106 68-69 -0.12599048926260892 1106 70-71 -0.08545888323065753 1106 72-73 0.4491017442605987 1106 74-75 0.4992146010618903 1106 76-77 0.5040248888270646 1106 78-79 0.7249857935315696 1106 80-81 0.6768201203540478 1106 82-83 0.7217511515370703 1106 84-85 0.7797371250117386 1106 86-87 0.922637777100018 1106 88-89 0.695197704649761 1106 90-91 0.9105857478358352 1106 92-93 0.7835028039620404 1106 94-95 0.7546534456392884 1106 96-97 0.8111877896398703 1106 98-99 0.67639619754409 1106 100-101 0.4117398213538319 1107 1 0.46683725130624154 1107 2 0.4505482073906464 1107 3 0.37004913490045865 1107 4 0.35831745471149645 1107 5 0.41382395831474383 1107 6 0.33046465660969204 1107 7 0.32767626917518555 1107 8 0.30592705436177425 1107 9 0.4620036718755358 1107 10-11 0.5591140019821523 1107 12-13 0.4755913822053017 1107 14-15 0.5432096358877558 1107 16-17 0.6356515057629082 1107 18-19 0.5620907268526665 1107 20-21 0.6192283135177874 1107 22-23 0.6423155421979132 1107 24-25 0.6651893459248654 1107 26-27 0.5907973892414944 1107 28-29 0.6367112507791433 1107 30-31 0.7467523850487439 1107 32-33 0.66089516048077 1107 34-35 0.7082504292459575 1107 36-37 0.6575988380791316 1107 38-39 0.6466238794200692 1107 40-41 0.6573300807457656 1107 42-43 0.7620539820714427 1107 44-45 0.8544615000199087 1107 46-47 0.7827735393787805 1107 48-49 0.721126520008184 1107 50-51 0.591108217344555 1107 52-53 0.7459181089286133 1107 54-55 0.6911614968549955 1107 56-57 0.7593276885608802 1107 58-59 0.7731392030143738 1107 60-61 0.722669071602752 1107 62-63 0.7704424671755845 1107 64-65 0.7107533622571331 1107 66-67 0.7568811097716512 1107 68-69 0.7123353400812995 1107 70-71 0.8549752768876964 1107 72-73 0.908492545963405 1107 74-75 0.9678079941204096 1107 76-77 1.064867730375596 1107 78-79 1.0586509246380942 1107 80-81 1.0129485321245468 1107 82-83 0.9146679671645437 1107 84-85 0.9882310094108782 1107 86-87 0.8234666183206905 1107 88-89 0.8840997183726742 1107 90-91 1.0335442406807793 1107 92-93 1.0091703329825599 1107 94-95 0.7700555784740466 1107 96-97 0.8819170506318166 1107 98-99 0.809274133974828 1107 100-101 0.5225239930766463 1108 1 0.2805540509385338 1108 2 0.2796795351135266 1108 3 0.28729818736077917 1108 4 0.4000690260405335 1108 5 0.43573392106977593 1108 6 0.38813270582704007 1108 7 0.3368879234572617 1108 8 0.2973939078004548 1108 9 0.24567133441643563 1108 10-11 0.4451400260363414 1108 12-13 0.5006607833310497 1108 14-15 0.5847204464126463 1108 16-17 0.5207458584628881 1108 18-19 0.5213758861378253 1108 20-21 0.4376249924778435 1108 22-23 0.5222532362201164 1108 24-25 0.5867560421394487 1108 26-27 0.5803399801925408 1108 28-29 0.2886003111189126 1108 30-31 0.35631897912237775 1108 32-33 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-0.9864652823515812 2316 82-83 -0.9026969153943796 2316 84-85 -0.9341523094690452 2316 86-87 -1.123775960764025 2316 88-89 -1.2823968082033943 2316 90-91 -1.2976217612168064 2316 92-93 -1.2712946676161927 2316 94-95 -1.5222978554058848 2316 96-97 -1.621467382849385 2316 98-99 -1.5186514640870534 2316 100-101 -1.2252309472915268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12992.0 3 4898.0 4 5068.0 5 5759.0 6 6721.0 7 8295.0 8 10696.0 9 13501.0 10 16712.0 11 20421.0 12 24674.0 13 28705.0 14 32544.0 15 37202.0 16 42044.0 17 47979.0 18 46669.0 19 44619.0 20 47419.0 21 51447.0 22 56872.0 23 62968.0 24 70018.0 25 77798.0 26 87688.0 27 98493.0 28 112604.0 29 129261.0 30 148573.0 31 173198.0 32 203798.0 33 243564.0 34 295691.0 35 359980.0 36 424616.0 37 416972.0 38 192251.0 39 9868.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.291121215783186 18.44635802164354 14.788380651586767 23.474140110986507 2 15.6033878977267 19.65195346775703 40.21882238653863 24.525836247977637 3 19.505413978383316 23.522747208562116 28.975844405192454 27.995994407862117 4 13.058223769254932 16.241446074204717 36.46604732688712 34.23428282965323 5 14.20322406979963 37.06576845198006 33.795763504279655 14.935243973940656 6 33.10475534489956 35.29857000709492 17.27729176498027 14.31938288302525 7 29.580240833634775 31.840562119475447 20.011015327757754 18.568181719132024 8 26.677705372775602 34.71031159331075 19.396898775482633 19.215084258431016 9 26.50660007076904 16.789177730798176 19.749382813910938 36.95483938452185 10-11 25.28396132500893 26.29403894711996 27.074277976532358 21.34772175133875 12-13 26.246847563657184 23.47331646586094 27.270949290396235 23.008886680085645 14-15 22.958567552016124 25.100416401379604 25.589012178804044 26.35200386780022 16-17 22.7105836004419 28.158277351299883 26.29805502835963 22.83308401989859 18-19 22.66052321907211 26.75872758177341 28.43572261326244 22.145026585892044 20-21 23.311383178491756 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28.071676432478256 22.74039696706845 46-47 22.961445736542828 26.71243732212391 27.368933014428336 22.957183926904925 48-49 22.83172629693937 26.627219660017037 27.515859771732572 23.025194271311022 50-51 23.034154350099005 26.830976221561926 27.37014077588011 22.76472865245896 52-53 23.13674514155066 26.621065936248932 27.364237025583726 22.87795189661668 54-55 22.748271435984275 26.72485710523854 27.543876680857544 22.98299477791964 56-57 22.87086226977134 26.893616024734545 27.307297142668858 22.928224562825257 58-59 22.902176288416253 26.52305128339541 27.356708560349162 23.218063867839174 60-61 22.652396188493725 26.70212500883327 27.587221980087396 23.05825682258561 62-63 22.653873341836317 26.696462511531855 27.720129091425967 22.929535055205864 64-65 22.497123083031116 26.61091011874192 27.606693726659937 23.28527307156702 66-67 22.476525430086305 26.711580597214677 27.45881032770795 23.353083644991063 68-69 22.780023293576676 26.44824422691549 27.646730607362173 23.12500187214566 70-71 22.611847557616084 26.487726189150113 27.45633498476617 23.444091268467638 72-73 22.724042381297412 26.539007322047397 27.384805856330672 23.35214444032452 74-75 22.659777681671265 26.81318582557249 27.219792627807436 23.307243864948806 76-77 22.759113777187267 26.538061594220164 27.343244611836703 23.359580016755867 78-79 22.791049449950986 26.599703191373486 27.365319681951856 23.24392767672367 80-81 22.81338184361018 26.803634404028593 27.10333358839261 23.279650163968622 82-83 22.872959408949008 26.760921785324943 27.168029068938658 23.198089736787395 84-85 22.887412681385204 26.62482026392288 27.193345166653348 23.294421888038567 86-87 22.873028224195107 26.6573672984927 27.087330325675314 23.382274151636885 88-89 22.882658216746552 26.643804163622963 26.991834975979934 23.48170264365055 90-91 22.893519738584843 26.664389512178214 27.008645696416355 23.433445052820588 92-93 22.875640411633714 26.266494306067706 27.221227316222645 23.636637966075938 94-95 22.76057971503976 26.323038918545105 27.27480845649965 23.641572909915485 96-97 23.048322834093398 26.049754889109366 27.139424140920205 23.76249813587703 98-99 22.78834543838055 26.144478119883747 26.917065958243274 24.15011048349243 100-101 23.423451668030005 25.58425248927608 27.147902399740246 23.844393442953674 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 522.0 1 366.0 2 256.0 3 317.0 4 286.0 5 297.5 6 551.0 7 1256.0 8 2014.5 9 2278.5 10 2122.5 11 1971.0 12 2022.5 13 2176.5 14 2423.5 15 2670.5 16 2918.0 17 3217.0 18 3511.0 19 3869.5 20 4470.0 21 5269.5 22 6448.0 23 7957.0 24 9624.0 25 12174.5 26 15733.0 27 19903.0 28 23985.5 29 28858.0 30 34860.0 31 42670.5 32 52930.0 33 59710.0 34 64459.0 35 71105.5 36 79146.5 37 87350.5 38 93725.5 39 98807.0 40 104031.0 41 111519.0 42 118156.5 43 123977.0 44 130076.5 45 136447.0 46 140816.5 47 143446.5 48 144515.0 49 142807.0 50 140634.0 51 136884.0 52 133487.5 53 133220.5 54 133324.0 55 125026.5 56 112453.5 57 101852.5 58 93767.0 59 83245.0 60 68111.0 61 56666.0 62 47564.0 63 36838.0 64 27762.5 65 22190.5 66 17169.5 67 13069.5 68 9996.0 69 7393.0 70 5650.0 71 4174.5 72 3162.0 73 2635.5 74 2010.5 75 1459.5 76 1065.0 77 684.5 78 442.0 79 313.5 80 209.5 81 115.0 82 65.0 83 47.5 84 35.5 85 26.5 86 19.0 87 12.0 88 7.0 89 4.5 90 6.5 91 4.5 92 1.0 93 1.0 94 2.5 95 2.5 96 2.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18319551465060382 2 0.04759598091695237 3 0.04658851450166218 4 0.05162584657811309 5 0.059549433790260196 6 0.06401496060397883 7 0.03624156212841167 8 0.03417217165376157 9 0.040353114255677 10-11 0.04151033919215897 12-13 0.03840625348018382 14-15 0.033804582556290826 16-17 0.031667120026553544 18-19 0.039726851348875 20-21 0.05004657490009064 22-23 0.046819959488958576 24-25 0.06778615245580831 26-27 0.05232018154000227 28-29 0.044287679039715686 30-31 0.05374969469683293 32-33 0.0493658543492189 34-35 0.04893019319666098 36-37 0.04669742978980166 38-39 0.025962681810248328 40-41 0.023920520157633098 42-43 0.02053053181429181 44-45 0.004315768292526859 46-47 0.011708393474994003 48-49 0.010755384703773562 50-51 0.008808523928280371 52-53 0.009325871546942898 54-55 0.010442253250372558 56-57 0.008332019542670151 58-59 0.010523939716477167 60-61 0.010415024428337688 62-63 0.0038937215509863778 64-65 0.006671061398543094 66-67 0.004016251250143292 68-69 0.008767680695228068 70-71 0.022273176424523474 72-73 0.01970005274222828 74-75 0.019795353619350326 76-77 0.011776465530081177 78-79 0.0048739591442416895 80-81 0.006262629068020048 82-83 0.005595522928165738 84-85 0.004914802377293994 86-87 0.00780105751299019 88-89 0.028998695467136314 90-91 0.03622794771739424 92-93 0.02231401965757578 94-95 0.031435675039257156 96-97 0.03489373543768562 98-99 0.02078920562362307 100-101 0.1223799406357222 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3672579.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.71837347739249 #Duplication Level Percentage of deduplicated Percentage of total 1 76.46230003700305 41.07430390328171 2 12.900506584807466 13.859884615404972 3 4.431061538521948 7.140882558828941 4 2.034322239976822 4.371219274417623 5 1.1031768090114853 2.9630431919038522 6 0.6813925900293161 2.1961980981553553 7 0.42185573384797187 1.5862982705097388 8 0.30867079592635377 1.3265034477708697 9 0.242410071119612 1.1719687261576133 >10 1.2604587743537243 12.618955428699289 >50 0.10050347180662886 3.712086523203822 >100 0.04880645355477091 4.796294103773832 >500 0.0025476966652930888 0.9306352200675336 >1k 0.001834341577382931 1.63561875609134 >5k 1.528617981152442E-4 0.6161078817333818 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9851 0.2682311258655022 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7400 0.20149328305803632 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5258 0.1431691462593453 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 2.7228822034869774E-5 0.0 9 0.0 0.0 0.0 5.445764406973955E-5 0.0 10-11 0.0 0.0 0.0 5.445764406973955E-5 0.0 12-13 0.0 0.0 0.0 5.445764406973955E-5 0.0 14-15 0.0 0.0 0.0 5.445764406973955E-5 0.0 16-17 0.0 0.0 0.0 6.807205508717444E-5 0.0 18-19 0.0 0.0 0.0 9.530087712204421E-5 0.0 20-21 0.0 0.0 0.0 1.4975852119178377E-4 0.0 22-23 0.0 0.0 0.0 2.314449872963931E-4 0.0 24-25 0.0 0.0 0.0 5.445764406973955E-4 0.0 26-27 0.0 0.0 0.0 0.0011572249364819654 0.0 28-29 0.0 0.0 0.0 0.003267458644184373 0.0 30-31 0.0 0.0 0.0 0.010469482072407428 0.0 32-33 0.0 0.0 0.0 0.025336418903446324 0.0 34-35 0.0 0.0 0.0 0.04664297214573192 0.0 36-37 0.0 0.0 0.0 0.08525344179117726 0.0 38-39 0.0 0.0 0.0 0.14600094375097172 0.0 40-41 0.0 0.0 0.0 0.21121397252448484 0.0 42-43 0.0 0.0 0.0 0.2798169896413392 0.0 44-45 0.0 0.0 0.0 0.36422633794943554 0.0 46-47 0.0 0.0 0.0 0.4661982764700229 0.0 48-49 0.0 0.0 0.0 0.574800433156101 0.0 50-51 0.0 0.0 0.0 0.6945527924654582 0.0 52-53 0.0 0.0 0.0 0.8149722579146699 0.0 54-55 0.0 2.7228822034869774E-5 0.0 0.9457795189701841 0.0 56-57 0.0 6.807205508717444E-5 0.0 1.100738745170628 0.0 58-59 0.0 8.168646610460933E-5 0.0 1.2678420259986238 0.0 60-61 0.0 8.168646610460933E-5 0.0 1.454958491022249 0.0 62-63 0.0 8.168646610460933E-5 0.0 1.641571222838229 0.0 64-65 0.0 8.168646610460933E-5 0.0 1.8379863305867619 0.0 66-67 0.0 8.168646610460933E-5 0.0 2.06213399357781 0.0 68-69 0.0 8.168646610460933E-5 0.0 2.31355132183678 0.0 70-71 0.0 8.168646610460933E-5 0.0 2.581578231537021 0.0 72-73 0.0 8.168646610460933E-5 0.0 2.8541931977501367 0.0 74-75 0.0 8.168646610460933E-5 0.0 3.1313962204761285 0.0 76-77 0.0 8.168646610460933E-5 0.0 3.43959925708882 0.0 78-79 0.0 8.168646610460933E-5 0.0 3.7824101265078305 0.0 80-81 0.0 8.168646610460933E-5 0.0 4.14204840794439 0.0 82-83 0.0 8.168646610460933E-5 0.0 4.52201300502998 0.0 84-85 0.0 8.168646610460933E-5 0.0 4.906184455119957 0.0 86-87 0.0 8.168646610460933E-5 0.0 5.304909710587573 0.0 88-89 0.0 8.168646610460933E-5 0.0 5.732225229191803 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5820 0.0 50.636246 1 GTATCAA 10450 0.0 43.6003 1 ATCAACG 13560 0.0 33.43631 3 TATCAAC 13665 0.0 33.0746 2 TCAACGC 13710 0.0 33.035824 4 CAACGCA 13920 0.0 32.39866 5 AACGCAG 14610 0.0 30.896435 6 ACGCAGA 16800 0.0 26.526217 7 CGCAGAG 16965 0.0 26.268225 8 TGGTATC 2865 0.0 25.7117 2 GTACATG 13745 0.0 25.424074 1 TACATGG 13680 0.0 25.04799 2 GTGGTAT 3390 0.0 24.296305 1 GCAGAGT 18880 0.0 22.998333 9 ACATGGG 14435 0.0 22.882195 3 GAGTACT 10320 0.0 21.171295 12-13 TAAGGTG 1950 0.0 20.471268 5 AGTACTT 10745 0.0 20.466515 12-13 AGAGTAC 17420 0.0 20.437803 10-11 CAGAGTA 17820 0.0 19.76579 10-11 >>END_MODULE