FastQCFastQC Report
Thu 26 May 2016
SRR938574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938574_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3672579
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98280.2676048629587001No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77050.2097980737786716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54720.1489961141748074No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59450.053.7609631
GTATCAA101950.042.5925031
ATCAACG134300.032.0342833
TCAACGC136900.031.4606154
TATCAAC137750.031.3159312
CAACGCA141400.030.4593945
AACGCAG148800.028.982446
TGGTATC27900.025.399882
ACGCAGA171900.024.9496177
CGCAGAG172850.024.7850178
GGACCGA11900.023.5481666
GTACATG138050.023.0827751
TACATGG135200.022.8306162
GTGGTAT34200.022.806961
GCAGAGT194900.021.6154259
ACATGGG138650.021.5318933
TAAGGTG19800.021.1280755
GAGTACT107400.020.65211912-13
AGAGTAC178400.019.58122610-11
GTAAGGT18750.019.522344
Produced by FastQC (version 0.11.5)