FastQCFastQC Report
Thu 26 May 2016
SRR938573_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938573_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3673216
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98070.266986749486009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74070.20164890929365442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52900.1440154894239816No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57550.050.5083351
GTATCAA104450.044.8916171
TATCAAC136150.034.1724742
ATCAACG135950.034.0829163
TCAACGC136600.033.9207344
CAACGCA139750.033.156165
AACGCAG146450.031.6691426
ACGCAGA168950.027.3316357
CGCAGAG170500.027.027448
GTACATG137700.025.4004081
TGGTATC28050.024.3972552
TACATGG140250.024.1939452
GCAGAGT187450.023.8969449
GTGGTAT31300.022.988531
ACATGGG145550.022.4313763
GAGTACT101800.021.71978612-13
TAGCCCT24900.021.3761774
AGAGTAC172100.020.82403810-11
AGTACTT104600.020.7069812-13
GTAAGGT16550.020.6750164
Produced by FastQC (version 0.11.5)