##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938573_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3673216 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.000216159354636 31.0 31.0 34.0 28.0 34.0 2 31.140598592622922 31.0 31.0 34.0 27.0 34.0 3 31.19691899414573 31.0 31.0 34.0 27.0 34.0 4 34.86089655495348 37.0 35.0 37.0 32.0 37.0 5 34.86452144387915 37.0 35.0 37.0 32.0 37.0 6 34.86301949027773 37.0 35.0 37.0 32.0 37.0 7 34.77435958026972 37.0 35.0 37.0 32.0 37.0 8 34.72774756507649 37.0 35.0 37.0 31.0 37.0 9 36.197780092431266 39.0 35.0 39.0 32.0 39.0 10-11 36.049035912943864 39.0 35.0 39.0 30.0 39.0 12-13 35.866389153265146 38.0 35.0 39.0 30.0 39.0 14-15 36.82690454359341 39.0 36.0 41.0 30.5 41.0 16-17 36.64081733826707 39.0 36.0 40.5 30.0 41.0 18-19 36.495449219430604 39.0 36.0 40.0 30.0 41.0 20-21 36.474717794978574 39.0 36.0 40.5 29.5 41.0 22-23 36.57160727819981 39.0 36.0 41.0 30.0 41.0 24-25 36.47702381237586 39.0 36.0 41.0 29.5 41.0 26-27 36.27215742281423 39.0 35.0 41.0 29.0 41.0 28-29 36.07726839369098 39.0 35.0 40.0 28.0 41.0 30-31 35.80799481979824 38.5 35.0 40.0 27.0 41.0 32-33 35.49601289442276 38.0 34.0 40.0 27.0 41.0 34-35 35.2277829564066 38.0 34.0 40.0 25.5 41.0 36-37 34.927691701223125 38.0 34.0 40.0 25.0 41.0 38-39 34.59393280983204 38.0 33.0 40.0 24.5 41.0 40-41 34.478578308490434 38.0 33.0 40.0 24.0 41.0 42-43 34.24089217731819 38.0 33.0 40.0 23.5 41.0 44-45 33.917811122460535 38.0 33.0 40.0 22.5 41.0 46-47 33.513043883071404 38.0 32.5 40.0 20.0 41.0 48-49 33.11607580387323 37.0 31.5 40.0 18.0 41.0 50-51 32.3334590179287 36.5 30.5 39.5 15.0 40.5 52-53 32.34210266970415 36.5 31.0 39.0 14.5 40.0 54-55 32.38534447742796 37.0 31.0 39.5 12.5 41.0 56-57 32.02389813177336 36.0 30.5 39.0 8.5 41.0 58-59 31.56075425458236 36.0 30.0 39.0 7.5 41.0 60-61 31.04452773809109 35.5 29.0 39.0 4.0 40.0 62-63 30.42808672291529 35.0 28.0 38.5 2.0 40.0 64-65 29.905237535718022 34.5 28.0 38.0 2.0 40.0 66-67 29.318450099313516 34.0 26.5 38.0 2.0 40.0 68-69 28.663922840366588 34.0 26.0 37.0 2.0 39.5 70-71 27.99419500513991 33.5 25.5 36.5 2.0 39.0 72-73 27.33455220166742 33.0 24.5 36.0 2.0 39.0 74-75 26.60410005836847 32.5 22.0 35.5 2.0 38.5 76-77 25.831518211833988 32.0 19.5 35.0 2.0 37.5 78-79 25.007389301364256 31.0 16.5 35.0 2.0 37.0 80-81 24.21532602493292 31.0 9.0 34.5 2.0 36.0 82-83 23.456320428746906 30.0 4.5 34.0 2.0 36.0 84-85 22.697708084686553 30.0 2.0 34.0 2.0 35.0 86-87 21.948518001663935 29.0 2.0 34.0 2.0 35.0 88-89 21.16058462121476 29.0 2.0 34.0 2.0 35.0 90-91 20.327449978438516 27.5 2.0 34.0 2.0 35.0 92-93 19.44619918893961 26.0 2.0 33.5 2.0 35.0 94-95 18.53779834891278 25.0 2.0 33.0 2.0 35.0 96-97 17.460131394396626 21.5 2.0 32.5 2.0 35.0 98-99 16.219343485381746 8.5 2.0 32.0 2.0 34.0 100-101 13.541143782451126 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6337064800918242 1101 2 0.6741495978597634 1101 3 0.6573013711021432 1101 4 0.4758106708104677 1101 5 0.573450922636475 1101 6 0.4667428833724969 1101 7 0.44350573756483413 1101 8 0.5891442904811015 1101 9 0.62542661350804 1101 10-11 0.71514750771248 1101 12-13 0.7898163332962582 1101 14-15 0.9941969957479202 1101 16-17 1.118230756266449 1101 18-19 1.2107807089502884 1101 20-21 1.134012714113787 1101 22-23 0.9927758585605702 1101 24-25 1.041685207889202 1101 26-27 1.113887582786738 1101 28-29 1.1797280780397656 1101 30-31 1.1807052170338608 1101 32-33 1.301615658743799 1101 34-35 1.4194895275017885 1101 36-37 1.2728078719180047 1101 38-39 1.4828903273104928 1101 40-41 1.566333701504199 1101 42-43 1.7416341645873956 1101 44-45 1.682070679184612 1101 46-47 1.9526667072169843 1101 48-49 1.9734691202598498 1101 50-51 1.8728699435176352 1101 52-53 2.034352027264511 1101 54-55 2.3131065646298765 1101 56-57 2.4555693199984603 1101 58-59 2.529230455828298 1101 60-61 2.533645999643486 1101 62-63 2.5071541643618396 1101 64-65 2.641661346340072 1101 66-67 2.882189707494721 1101 68-69 3.032391210038128 1101 70-71 3.133675269169917 1101 72-73 3.432033419731649 1101 74-75 3.6385280000361355 1101 76-77 3.794258014052815 1101 78-79 4.077938748043646 1101 80-81 4.413626727415206 1101 82-83 4.532299467176355 1101 84-85 4.653562465863498 1101 86-87 4.93205330975211 1101 88-89 5.17296090293717 1101 90-91 5.267313970400359 1101 92-93 5.4790441745478375 1101 94-95 5.653422356034934 1101 96-97 5.9181822790257 1101 98-99 6.0300351925900415 1101 100-101 5.970526814365568 1102 1 0.6177216342158118 1102 2 0.6491719608896354 1102 3 0.6854819495946707 1102 4 0.6295623446065193 1102 5 0.5960740573801147 1102 6 0.640357436069479 1102 7 0.5999893027134107 1102 8 0.701044283776767 1102 9 0.7022423942127674 1102 10-11 0.7743536396993562 1102 12-13 0.8612790858961858 1102 14-15 1.1107438577951854 1102 16-17 1.2160095588258102 1102 18-19 1.0905263335405166 1102 20-21 1.204114262315862 1102 22-23 0.8925122933887053 1102 24-25 0.8374482767240821 1102 26-27 0.9786844140156887 1102 28-29 0.9715965193752609 1102 30-31 1.0537030874512254 1102 32-33 1.0886839299921718 1102 34-35 1.0822901391100643 1102 36-37 1.3683345672286436 1102 38-39 1.275621348596836 1102 40-41 1.5381439731761262 1102 42-43 1.6226181362382093 1102 44-45 1.815067614655156 1102 46-47 1.871540605348038 1102 48-49 1.7622099814457002 1102 50-51 1.9449680274256167 1102 52-53 2.076321615872935 1102 54-55 2.2539497585746133 1102 56-57 2.297096123110798 1102 58-59 2.3567495677552444 1102 60-61 2.4151209431515674 1102 62-63 2.725202314789126 1102 64-65 2.789665925250503 1102 66-67 2.9062230398465267 1102 68-69 3.1384923702871816 1102 70-71 3.0770161186772462 1102 72-73 3.168801052338136 1102 74-75 3.1878159182439276 1102 76-77 3.4806426594975193 1102 78-79 3.877318330967526 1102 80-81 4.26396490533692 1102 82-83 4.404051379533573 1102 84-85 4.592939315481953 1102 86-87 4.843977200754608 1102 88-89 5.118130430258333 1102 90-91 5.4937996832648786 1102 92-93 5.416584319451285 1102 94-95 5.59074569142615 1102 96-97 5.653468012585485 1102 98-99 5.929076614228272 1102 100-101 5.831705966981 1103 1 0.5869178585265402 1103 2 0.5718413533093099 1103 3 0.44349644547029854 1103 4 0.5477776000914929 1103 5 0.3860756902091609 1103 6 0.5014061633024269 1103 7 0.5716469248505405 1103 8 0.43741196041456476 1103 9 0.5300706468250169 1103 10-11 0.5713003533390477 1103 12-13 0.6374990258893121 1103 14-15 0.7973351968285485 1103 16-17 0.8630638856548458 1103 18-19 1.0242441893177343 1103 20-21 1.045104620308436 1103 22-23 0.9085593566073342 1103 24-25 0.8132909228602827 1103 26-27 0.9780315622661746 1103 28-29 1.0422143764478662 1103 30-31 1.0251170646277643 1103 32-33 1.1176845570561014 1103 34-35 1.203397916861725 1103 36-37 1.2180781522975224 1103 38-39 1.2860388298788692 1103 40-41 1.3236765161306394 1103 42-43 1.5074900551129957 1103 44-45 1.4508918039059537 1103 46-47 1.4854161178860323 1103 48-49 1.5905539905383606 1103 50-51 1.7159938598685756 1103 52-53 1.8173613665442545 1103 54-55 1.900182833166113 1103 56-57 2.0376282734507 1103 58-59 2.1688378121697625 1103 60-61 2.3774896711329667 1103 62-63 2.577030995359305 1103 64-65 2.8064448029356086 1103 66-67 2.80521061387358 1103 68-69 2.924800387093967 1103 70-71 3.1798332925204456 1103 72-73 3.2574271266508923 1103 74-75 3.58131660296063 1103 76-77 3.6594494340908277 1103 78-79 3.807739063514447 1103 80-81 4.159919111598889 1103 82-83 4.60434328970527 1103 84-85 4.748281089310023 1103 86-87 4.663239297962793 1103 88-89 5.042324302879436 1103 90-91 5.3305310122006055 1103 92-93 5.27641354248534 1103 94-95 5.474142866615914 1103 96-97 5.816892410121945 1103 98-99 6.014854233467574 1103 100-101 5.674942424455237 1104 1 0.4957044293746655 1104 2 0.4453535511604443 1104 3 0.3962775045839244 1104 4 0.40276085125370287 1104 5 0.3745129045453055 1104 6 0.4106554062955894 1104 7 0.3920804008139527 1104 8 0.46766918732618024 1104 9 0.5679685217475168 1104 10-11 0.5661888988564172 1104 12-13 0.6847596247051797 1104 14-15 0.7041226040193038 1104 16-17 0.849970824948592 1104 18-19 1.0131639381784154 1104 20-21 0.8633320075579718 1104 22-23 0.8628952913058967 1104 24-25 1.004319593977634 1104 26-27 1.043071833619635 1104 28-29 0.9420064355291942 1104 30-31 1.0954046809799962 1104 32-33 1.1491064244442413 1104 34-35 1.1854600275837228 1104 36-37 1.333263729844731 1104 38-39 1.3210205360208747 1104 40-41 1.4865252847313357 1104 42-43 1.4980023574311474 1104 44-45 1.52524590131015 1104 46-47 1.7044220876682346 1104 48-49 1.822153732357755 1104 50-51 1.8190086506699572 1104 52-53 1.9420596213688768 1104 54-55 2.1598327276453304 1104 56-57 2.3425008638673575 1104 58-59 2.355162548731684 1104 60-61 2.498192858499035 1104 62-63 2.6102943782439674 1104 64-65 2.674844219618464 1104 66-67 2.8193272694188245 1104 68-69 2.972111134627511 1104 70-71 3.1520845249356064 1104 72-73 3.2518091814913745 1104 74-75 3.2814329949882044 1104 76-77 3.656740607325194 1104 78-79 3.7886601487645386 1104 80-81 4.152902574424324 1104 82-83 4.473292257490762 1104 84-85 4.710767687889668 1104 86-87 4.699885653451169 1104 88-89 5.055972827915813 1104 90-91 5.156316262276302 1104 92-93 5.18504614198714 1104 94-95 5.28329515542158 1104 96-97 5.674511671960111 1104 98-99 5.923162932143601 1104 100-101 5.598446487828054 1105 1 0.6325212719546194 1105 2 0.6036627847638663 1105 3 0.507758965347513 1105 4 0.42125457487443896 1105 5 0.4502837208233288 1105 6 0.5483388613103344 1105 7 0.5888926647927448 1105 8 0.498900596645214 1105 9 0.6098496365851318 1105 10-11 0.6741852877435903 1105 12-13 0.6932496296504738 1105 14-15 0.9125304558601428 1105 16-17 0.986871467146301 1105 18-19 1.0025995781306847 1105 20-21 1.0293556952041953 1105 22-23 0.9139888277849408 1105 24-25 0.9324432130454952 1105 26-27 1.1108940889862282 1105 28-29 1.1722971680751826 1105 30-31 1.092919560355277 1105 32-33 1.1662401927926211 1105 34-35 1.335243521870325 1105 36-37 1.3487178748808333 1105 38-39 1.4620736991311034 1105 40-41 1.4403047086069947 1105 42-43 1.6903570281867317 1105 44-45 1.7712579598270892 1105 46-47 1.8826793331535896 1105 48-49 1.8336301457312416 1105 50-51 1.7764984095729588 1105 52-53 1.9101234108889784 1105 54-55 2.096215336496037 1105 56-57 2.3229435214174643 1105 58-59 2.2675327384143316 1105 60-61 2.4700119925365733 1105 62-63 2.7110126391117895 1105 64-65 2.949266901007789 1105 66-67 3.1663435716260544 1105 68-69 3.1712769779176924 1105 70-71 3.4268160893432515 1105 72-73 3.4761658873086283 1105 74-75 3.571919031333156 1105 76-77 4.008641230109863 1105 78-79 4.205584889014482 1105 80-81 4.461409806644099 1105 82-83 4.859689778431317 1105 84-85 5.004571412575963 1105 86-87 5.228111672704017 1105 88-89 5.47216900400732 1105 90-91 5.773208568371324 1105 92-93 5.922286778395449 1105 94-95 6.050663569889675 1105 96-97 6.257010759906784 1105 98-99 6.435911366913416 1105 100-101 5.889386969032708 1106 1 0.24577558584325843 1106 2 0.3583800889890867 1106 3 0.3225013994871695 1106 4 0.2390314565711833 1106 5 0.2416375084482496 1106 6 0.13053049736348754 1106 7 0.3283690921892912 1106 8 0.2772656991799778 1106 9 0.2675091125597149 1106 10-11 0.19957299265674777 1106 12-13 0.11759808356319468 1106 14-15 0.5589201604048881 1106 16-17 0.3673728990011611 1106 18-19 0.4648882273752122 1106 20-21 0.4784077205180992 1106 22-23 0.38883326326857315 1106 24-25 0.5075906419007339 1106 26-27 0.6534687810544995 1106 28-29 0.48471988198127036 1106 30-31 0.7445729409248827 1106 32-33 1.0320016408786827 1106 34-35 0.9197350674102154 1106 36-37 1.0598641820107417 1106 38-39 0.9707705811631584 1106 40-41 0.9701246113645254 1106 42-43 0.6115271289908293 1106 44-45 0.9411897814822154 1106 46-47 1.260469666096654 1106 48-49 1.355906309548132 1106 50-51 0.7009706716972985 1106 52-53 1.1869152965217111 1106 54-55 1.1346974793467766 1106 56-57 1.2283904450592544 1106 58-59 0.6613351701709718 1106 60-61 1.9238906036004586 1106 62-63 2.1464905771404723 1106 64-65 2.17265359868955 1106 66-67 2.391451619009352 1106 68-69 1.9578947790308376 1106 70-71 2.3195251684903013 1106 72-73 2.6574435350328542 1106 74-75 2.9604213897434555 1106 76-77 3.104082816671969 1106 78-79 3.397843064100069 1106 80-81 3.8929967778646386 1106 82-83 4.078830635420584 1106 84-85 4.019179169461346 1106 86-87 4.236302717945527 1106 88-89 4.232137553282449 1106 90-91 4.820156563405522 1106 92-93 4.9193705504848815 1106 94-95 4.916699251731519 1106 96-97 5.138963678251901 1106 98-99 5.22322028724491 1106 100-101 4.682219111249369 1107 1 0.446799078780554 1107 2 0.2931043197512402 1107 3 0.30523585146382715 1107 4 0.3715048957371039 1107 5 0.33179890374283616 1107 6 0.3406204270055184 1107 7 0.3576398370276266 1107 8 0.32577070521137586 1107 9 0.31563157176690737 1107 10-11 0.4209563105223637 1107 12-13 0.4111507456083672 1107 14-15 0.7046223934301352 1107 16-17 0.7912668282202802 1107 18-19 0.7649740992359497 1107 20-21 0.7479280871304823 1107 22-23 0.7156336316946792 1107 24-25 0.714949108847307 1107 26-27 0.7753993011187532 1107 28-29 0.8215775512613916 1107 30-31 0.8902671969356888 1107 32-33 0.9703395641441404 1107 34-35 0.932027051889186 1107 36-37 1.0521156670474383 1107 38-39 1.1970273650366536 1107 40-41 1.0358926113751608 1107 42-43 1.1647709364310188 1107 44-45 1.3011954871467637 1107 46-47 1.236648726559693 1107 48-49 1.418984516267706 1107 50-51 1.531681526545654 1107 52-53 1.682594343724709 1107 54-55 1.681348284349994 1107 56-57 1.8564142146030278 1107 58-59 2.1076466091886914 1107 60-61 2.147233922896344 1107 62-63 2.3430028934758376 1107 64-65 2.5402878142317036 1107 66-67 2.7073779172737 1107 68-69 2.8573196712764144 1107 70-71 2.799078331128122 1107 72-73 3.216259776878019 1107 74-75 3.2435321048960937 1107 76-77 3.3982723103701638 1107 78-79 3.5447315631415357 1107 80-81 3.9475394741696945 1107 82-83 4.066722472142853 1107 84-85 4.218443940635435 1107 86-87 4.557814465743679 1107 88-89 4.753800290569764 1107 90-91 5.068351851355104 1107 92-93 5.184005765462967 1107 94-95 5.285863479635939 1107 96-97 5.416016445712625 1107 98-99 5.518002739016687 1107 100-101 5.31736408054171 1108 1 0.345676684275368 1108 2 0.4139614506309073 1108 3 0.4004829250783857 1108 4 0.38344176550817366 1108 5 0.31045089786910296 1108 6 0.32348751816869026 1108 7 0.289369463920643 1108 8 0.28292475741520917 1108 9 0.4048412690530441 1108 10-11 0.37709677119557483 1108 12-13 0.5199717005751694 1108 14-15 0.556854092838023 1108 16-17 0.6856904919963682 1108 18-19 0.6525179235760632 1108 20-21 0.6530785692139531 1108 22-23 0.662427328102396 1108 24-25 0.683325450448784 1108 26-27 0.6195696862192932 1108 28-29 0.7859461830770655 1108 30-31 0.7590725251754336 1108 32-33 0.9261763447151878 1108 34-35 1.1304605579337448 1108 36-37 1.1233660191223152 1108 38-39 1.062002999435343 1108 40-41 1.1078850939101628 1108 42-43 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0.6640498676940823 2311 50-51 0.7553277885793506 2311 52-53 0.8337053444170195 2311 54-55 0.805501923683714 2311 56-57 0.9923550212158645 2311 58-59 1.0848997083024088 2311 60-61 1.1286883740193865 2311 62-63 1.2226311271515975 2311 64-65 1.2644923695470744 2311 66-67 1.3819723964220785 2311 68-69 1.2972465502331687 2311 70-71 1.4818818533544338 2311 72-73 1.601748931049169 2311 74-75 1.762594583532831 2311 76-77 2.0054041911855016 2311 78-79 2.2186925633800527 2311 80-81 2.1997313653915285 2311 82-83 2.41817078631653 2311 84-85 2.743846105393885 2311 86-87 2.966719943860614 2311 88-89 2.7470618888262166 2311 90-91 3.091697951989449 2311 92-93 3.2106980346370513 2311 94-95 3.319512361903474 2311 96-97 3.18547295515242 2311 98-99 3.2122305264626405 2311 100-101 3.232594325183273 2312 1 -0.25742599603944427 2312 2 -0.2711284783404082 2312 3 -0.2727911962004157 2312 4 -0.31368968772994776 2312 5 -0.17841461473626907 2312 6 -0.04056658657442114 2312 7 -0.17240159223830176 2312 8 -0.2801680171520644 2312 9 -0.2536683848170398 2312 10-11 -0.24607629578714096 2312 12-13 -0.3099501024073703 2312 14-15 -0.17843512010875173 2312 16-17 -0.18519852167275275 2312 18-19 -0.08617457335729739 2312 20-21 -0.11729355223621951 2312 22-23 -0.1338462441770858 2312 24-25 -0.11532601308181967 2312 26-27 0.04648111761370899 2312 28-29 0.038326949497310636 2312 30-31 -0.04454855761200349 2312 32-33 -0.05876576264856226 2312 34-35 -0.044901346871490944 2312 36-37 0.02213172481267378 2312 38-39 0.07393997496973981 2312 40-41 0.1136473435840415 2312 42-43 0.2575541683100866 2312 44-45 0.12652469771310138 2312 46-47 0.1414405842600246 2312 48-49 0.36294936238518716 2312 50-51 0.5112751424548208 2312 52-53 0.5492560219625631 2312 54-55 0.6476425474338114 2312 56-57 0.7339632352453478 2312 58-59 1.042692665726328 2312 60-61 1.0345835011789468 2312 62-63 0.9467479081567483 2312 64-65 1.1045111544681987 2312 66-67 1.1395676050460999 2312 68-69 1.258117257992076 2312 70-71 1.4194725165159774 2312 72-73 1.29493015171019 2312 74-75 1.3891818196549046 2312 76-77 1.5026921462332687 2312 78-79 1.6776895761838553 2312 80-81 1.8753330286221015 2312 82-83 2.2296044311619028 2312 84-85 2.4585105548185133 2312 86-87 2.5855984593982946 2312 88-89 2.57924579415101 2312 90-91 2.754010834811961 2312 92-93 2.7072277272974965 2312 94-95 2.8473753428745816 2312 96-97 2.9322213545839375 2312 98-99 2.9503765010992566 2312 100-101 2.7614507850106325 2313 1 -0.5764179814198407 2313 2 -0.6050970958560953 2313 3 -0.5496777900597607 2313 4 -0.4384011153560792 2313 5 -0.38052434947017133 2313 6 -0.4075370933753746 2313 7 -0.37943753546563386 2313 8 -0.42437247629889185 2313 9 -0.5473985394999588 2313 10-11 -0.46905611672260505 2313 12-13 -0.4339110139844422 2313 14-15 -0.45127304756601205 2313 16-17 -0.4969152252062301 2313 18-19 -0.4417898450101134 2313 20-21 -0.5778439134274578 2313 22-23 -0.5040211022220191 2313 24-25 -0.5460230679499247 2313 26-27 -0.4162677641259904 2313 28-29 -0.3764772844331006 2313 30-31 -0.34255571763118553 2313 32-33 -0.3324735644433474 2313 34-35 -0.22645475742680077 2313 36-37 -0.24942159212829296 2313 38-39 -0.2295485820316614 2313 40-41 -0.02592126550636209 2313 42-43 0.011987115434983764 2313 44-45 -0.09227995427927027 2313 46-47 -0.06109765165650316 2313 48-49 0.1437782287130105 2313 50-51 0.17708135780673473 2313 52-53 0.19858093936638 2313 54-55 0.27655590415594844 2313 56-57 0.4170486876502153 2313 58-59 0.5090688261438743 2313 60-61 0.6310808281199023 2313 62-63 0.8151420920453738 2313 64-65 0.7195285704314642 2313 66-67 0.6605742326952928 2313 68-69 0.9247777042319498 2313 70-71 0.9959338179475168 2313 72-73 1.2005357909397247 2313 74-75 1.3905924092109423 2313 76-77 1.7178078387810984 2313 78-79 1.6849924804210623 2313 80-81 1.890595769595894 2313 82-83 1.9886596481045231 2313 84-85 2.044318571014781 2313 86-87 2.142364688614986 2313 88-89 2.219080557443448 2313 90-91 2.3892976038779423 2313 92-93 2.469397869150331 2313 94-95 2.639140304602325 2313 96-97 2.7141673726115982 2313 98-99 2.7849131482818237 2313 100-101 2.542767924047885 2314 1 -0.4389211254337475 2314 2 -0.5799916377004308 2314 3 -0.5795263758251394 2314 4 -0.46094083688311827 2314 5 -0.4043010238004854 2314 6 -0.38071764054075174 2314 7 -0.24188259359208786 2314 8 -0.38838006323778984 2314 9 -0.4986198552135406 2314 10-11 -0.4766480344125128 2314 12-13 -0.4339598798897555 2314 14-15 -0.49696939962476705 2314 16-17 -0.44661542826711553 2314 18-19 -0.41142143665193487 2314 20-21 -0.3460017462448448 2314 22-23 -0.42058238449287444 2314 24-25 -0.410073140566503 2314 26-27 -0.35069589657804556 2314 28-29 -0.24964840877673566 2314 30-31 -0.3706430102092497 2314 32-33 -0.28666251580683166 2314 34-35 -0.3310305608007269 2314 36-37 -0.24894086225975798 2314 38-39 -0.1377511206984181 2314 40-41 -0.07599969067440071 2314 42-43 0.027694737999894414 2314 44-45 0.062365238680207824 2314 46-47 0.23593937800454512 2314 48-49 0.3293536035421667 2314 50-51 0.33167750046125377 2314 52-53 0.3517483535891941 2314 54-55 0.3080331075657128 2314 56-57 0.4177697824530142 2314 58-59 0.7220780734171512 2314 60-61 0.8156043873870509 2314 62-63 0.9196917106700333 2314 64-65 0.8801194532705701 2314 66-67 1.2198084689230342 2314 68-69 1.257084392554507 2314 70-71 1.4417879976604588 2314 72-73 1.4790583588743935 2314 74-75 1.4963986192008285 2314 76-77 1.6839429365977345 2314 78-79 1.9725036089394976 2314 80-81 2.116141518778452 2314 82-83 2.1692789137366333 2314 84-85 2.383296654010845 2314 86-87 2.5643711079353473 2314 88-89 2.7152508534638784 2314 90-91 2.8749103749546343 2314 92-93 3.0965284226221677 2314 94-95 3.108119398108439 2314 96-97 3.174914220668505 2314 98-99 3.301205317714114 2314 100-101 3.2504199929012376 2315 1 -0.6497313177953821 2315 2 -0.5332963308518863 2315 3 -0.5511807154032127 2315 4 -0.3278769279411762 2315 5 -0.24359638674835793 2315 6 -0.33605371978842413 2315 7 -0.3577343437434095 2315 8 -0.4104617910970134 2315 9 -0.49143766527303256 2315 10-11 -0.5104135539956047 2315 12-13 -0.5144299331841182 2315 14-15 -0.6417920224158493 2315 16-17 -0.46135774044509503 2315 18-19 -0.46152367029086605 2315 20-21 -0.4913503002109181 2315 22-23 -0.41478606933277007 2315 24-25 -0.5519143794758108 2315 26-27 -0.4909896060721195 2315 28-29 -0.5657415336567837 2315 30-31 -0.5819983256203187 2315 32-33 -0.4998779667420621 2315 34-35 -0.5937289383491802 2315 36-37 -0.23484959751522894 2315 38-39 -0.18074719374564552 2315 40-41 -0.23949563823629916 2315 42-43 -0.202867952996165 2315 44-45 -0.15471368421274434 2315 46-47 -0.08861072016927096 2315 48-49 -0.13488086211175698 2315 50-51 0.06440592067472295 2315 52-53 0.2058336393266842 2315 54-55 0.3765632314271201 2315 56-57 0.5912698636494085 2315 58-59 0.7969540643822057 2315 60-61 0.7603554097234415 2315 62-63 0.881780377130017 2315 64-65 0.766794756280742 2315 66-67 1.0350929515543896 2315 68-69 1.209946565369954 2315 70-71 1.2455950012183372 2315 72-73 1.3181319080425027 2315 74-75 1.5028212538023027 2315 76-77 1.6507129441268553 2315 78-79 2.0054368655053345 2315 80-81 2.1531541558896308 2315 82-83 2.1859260194543353 2315 84-85 2.3002593184335396 2315 86-87 2.315868812291903 2315 88-89 2.671283376309596 2315 90-91 2.717608973947801 2315 92-93 2.808181950236321 2315 94-95 2.849390785976219 2315 96-97 2.983116901625735 2315 98-99 3.042912951651253 2315 100-101 2.8678480602794796 2316 1 -0.964871935948139 2316 2 -0.9561187472672188 2316 3 -0.9081239783630259 2316 4 -0.7412440302534549 2316 5 -0.8134762619835811 2316 6 -0.659333692979466 2316 7 -0.6269130615533243 2316 8 -0.6650360699617508 2316 9 -0.6757299344968644 2316 10-11 -0.7560487201338262 2316 12-13 -0.7619185264870367 2316 14-15 -1.3705704102693304 2316 16-17 -1.059488846572485 2316 18-19 -1.0074017926325283 2316 20-21 -0.6778607531276819 2316 22-23 -0.9577216504943209 2316 24-25 -1.0918642415643305 2316 26-27 -0.818144634041083 2316 28-29 -0.8870379015582088 2316 30-31 -0.7178720657280735 2316 32-33 -0.7000822561757261 2316 34-35 -0.577454739395769 2316 36-37 -0.5419133719075191 2316 38-39 -0.652294895650634 2316 40-41 -0.6377730336426879 2316 42-43 -0.48839562551439997 2316 44-45 -0.5251232941939321 2316 46-47 -0.3034735820257879 2316 48-49 -0.14431813172225105 2316 50-51 -0.039775890476775544 2316 52-53 0.06515297528935804 2316 54-55 0.00823131587534931 2316 56-57 0.12427388499346392 2316 58-59 0.2080913829706148 2316 60-61 0.14886686797622062 2316 62-63 0.47437375737903054 2316 64-65 0.5974336602067076 2316 66-67 0.7949123777395251 2316 68-69 1.034514141994606 2316 70-71 0.9915285459300094 2316 72-73 1.0742000131130034 2316 74-75 1.3098128502243362 2316 76-77 1.3476350692525223 2316 78-79 1.3087738077371789 2316 80-81 1.6500092586143502 2316 82-83 2.026635683568994 2316 84-85 1.963337064418642 2316 86-87 2.242348460682077 2316 88-89 2.2244276291387095 2316 90-91 2.350982262943237 2316 92-93 2.4206204056277585 2316 94-95 2.4476662480441185 2316 96-97 2.5537501344254707 2316 98-99 2.6077500356612546 2316 100-101 2.662198845214908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11924.0 3 4683.0 4 5106.0 5 5784.0 6 6984.0 7 8886.0 8 11274.0 9 14394.0 10 17893.0 11 22224.0 12 27033.0 13 31662.0 14 36155.0 15 41893.0 16 47142.0 17 53880.0 18 52782.0 19 52762.0 20 56844.0 21 61856.0 22 68097.0 23 75472.0 24 84062.0 25 93612.0 26 104266.0 27 115340.0 28 128426.0 29 142898.0 30 159089.0 31 178030.0 32 202664.0 33 232131.0 34 270209.0 35 315729.0 36 360883.0 37 363224.0 38 194483.0 39 13439.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29740587199913 18.42881616866829 14.755717474279079 23.518060485053496 2 15.632246701937863 19.626904550714354 40.258349221893994 24.48249952545379 3 19.51998187192526 23.535666360628305 28.961805486520646 27.982546280925785 4 13.015614313997265 16.297809627333507 36.412300252181154 34.27427580648808 5 14.267875909342756 37.05177090637086 33.71651986974872 14.963833314537661 6 33.124441928716145 35.27558029879661 17.284587889383257 14.315389883103984 7 29.61963943998577 31.848596492326912 19.977065025907372 18.55469904177994 8 26.733322821290646 34.716810744288026 19.39730592141186 19.15256051300947 9 26.517000818414182 16.810390728089036 19.739441980426147 36.933166473070635 10-11 25.247099477172654 26.282964014549353 27.101756551204932 21.368179957073067 12-13 26.277051726405798 23.401746680889644 27.32385383087604 22.99734776182852 14-15 22.94522156662112 25.102074300096465 25.570771219724403 26.38193291355801 16-17 22.73642938657388 28.155974816736233 26.298557988301507 22.80903780838838 18-19 22.701375959793836 26.77702610889768 28.392224652143238 22.129373279165247 20-21 23.308507390454515 26.002817123119225 28.56915255631319 22.11952293011307 22-23 22.945008883239055 25.88298633691386 28.451963517174445 22.72004126267264 24-25 22.734461747189734 26.010396537374785 28.627903027209435 22.627238688226043 26-27 22.322115678583476 26.50926570138702 28.353486109584747 22.81513251044476 28-29 22.450587234042555 26.77208510638298 28.27695659574468 22.50037106382979 30-31 22.605097800784478 26.362739497709274 28.25728799503796 22.774874706468285 32-33 22.32588893754507 26.33325653644965 28.253096929649697 23.08775759635558 34-35 23.020932775009804 26.475923829769332 28.01933353839509 22.483809856825772 36-37 23.045051816328783 26.105843923308292 27.91618159066802 22.932922669694904 38-39 22.574438622351963 26.431478455239887 28.009501959901762 22.98458096250639 40-41 23.095293299011953 26.167444579576454 28.003625827952067 22.73363629345953 42-43 22.716555447817544 26.212141139156575 27.936625541968947 23.134677871056933 44-45 22.70029425157795 26.487243312634302 28.046225336946307 22.76623709884144 46-47 22.98248148212186 26.679971502473933 27.341178911558423 22.99636810384579 48-49 22.86354184506732 26.62885866148389 27.471404389003233 23.036195104445557 50-51 23.049859576085993 26.854366835915904 27.314006548623727 22.781767039374373 52-53 23.166385926834852 26.635126882878534 27.339993950503576 22.858493239783034 54-55 22.764420658657976 26.73495566324623 27.504578381219442 22.996045296876353 56-57 22.883566048410326 26.92416699291972 27.261110618025832 22.931156340644122 58-59 22.930361692294394 26.537769104743674 27.32054749019349 23.21132171276844 60-61 22.69914038476382 26.71894119775479 27.55367201105478 23.028246406426607 62-63 22.69022710142428 26.699496780673176 27.665206345515802 22.945069772386738 64-65 22.526874445433364 26.614665253437803 27.545520408460533 23.3129398926683 66-67 22.528174988211642 26.727293665650638 27.40387180802349 23.340659538114235 68-69 22.82118328290135 26.450733936411595 27.618778339060817 23.10930444162623 70-71 22.746646612414178 26.481932657791095 27.35534908340013 23.41607164639459 72-73 22.74497022835884 26.544402608811783 27.344697976819006 23.36592918601037 74-75 22.71406260720692 26.802001958176913 27.164136719520016 23.319798715096148 76-77 22.815380466441145 26.55227059832362 27.28751636840402 23.34483256683121 78-79 22.868525928955037 26.581255965834995 27.31119102161232 23.239027083597648 80-81 22.922314929819507 26.75587360378202 27.044401270039025 23.277410196359448 82-83 22.9546614186692 26.755720348097316 27.11367944486526 23.17593878836822 84-85 23.01191865973476 26.63106333023329 27.108294328658584 23.24872368137337 86-87 23.011287956903622 26.62542155431457 27.033312574654673 23.32997791412713 88-89 23.019646994802308 26.6434635522358 26.941013270131986 23.395876182829902 90-91 23.030864584155964 26.616006339575783 26.95712616790617 23.396002908362085 92-93 23.013305506602382 26.3249043115777 27.09860723399708 23.563182947822835 94-95 22.889424111142272 26.31288445462244 27.189379726119906 23.60831170811538 96-97 23.154057734023116 26.086408389160827 27.10364478508589 23.65588909173017 98-99 22.947502581864786 26.17048394206712 26.858432126702375 24.023581349365717 100-101 23.502529770624136 25.63140703757251 27.15797678306927 23.70808640873408 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 523.0 1 352.5 2 257.0 3 361.5 4 332.0 5 323.5 6 558.5 7 1270.0 8 2073.0 9 2414.0 10 2239.5 11 2039.5 12 2118.0 13 2267.0 14 2457.0 15 2703.0 16 2965.5 17 3219.0 18 3545.0 19 4002.0 20 4591.5 21 5472.5 22 6521.5 23 7832.5 24 9737.0 25 12317.0 26 15915.0 27 20250.5 28 24399.5 29 29194.5 30 35225.5 31 43113.5 32 52958.5 33 59363.0 34 64323.5 35 71094.0 36 78601.5 37 86727.5 38 93389.0 39 98553.0 40 103839.5 41 111077.5 42 117487.5 43 123431.0 44 129131.0 45 135333.0 46 139868.5 47 142255.5 48 143282.0 49 141726.5 50 139797.0 51 136056.5 52 133040.5 53 132720.5 54 132014.0 55 124013.0 56 112166.0 57 101962.0 58 93872.5 59 83611.0 60 69198.5 61 57724.0 62 48291.0 63 38007.5 64 29530.5 65 23522.0 66 18024.0 67 13853.5 68 10675.0 69 8087.0 70 6268.5 71 4633.5 72 3465.5 73 2769.5 74 2147.0 75 1605.0 76 1144.0 77 772.5 78 531.0 79 343.0 80 216.5 81 135.5 82 78.0 83 46.0 84 28.5 85 22.0 86 22.5 87 18.0 88 8.0 89 5.0 90 5.5 91 3.5 92 3.0 93 2.5 94 2.0 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3000640310833885 2 0.03503741680315016 3 0.04255126842527093 4 0.04489254103216364 5 0.05874960797295885 6 0.056898369167508796 7 0.02981038958776179 8 0.03367621179914276 9 0.04031889221869882 10-11 0.03164801634317176 12-13 0.033295074398020694 14-15 0.027591625431229748 16-17 0.0211531257622748 18-19 0.02567232637557933 20-21 0.037705378611004634 22-23 0.030817681290727254 24-25 0.05165772990208036 26-27 0.04457946388124194 28-29 0.036507518207478135 30-31 0.036439457957277765 32-33 0.03245112729553612 34-35 0.031348551242290135 36-37 0.03024597518904415 38-39 0.019342723106944976 40-41 0.018389879604139805 42-43 0.019791920758267415 44-45 0.009215357877130015 46-47 0.01678365769941109 48-49 0.014619341743039343 50-51 0.006275155068474056 52-53 0.004982010314667038 54-55 0.006737964769836568 56-57 0.005390371815869255 58-59 0.00744579137192041 60-61 0.0064112755688747955 62-63 0.004505588563264453 64-65 0.008248902324284768 66-67 0.0027224100080147752 68-69 0.007677196222601665 70-71 0.013938739241035647 72-73 0.013870678990835278 74-75 0.011216329233020874 76-77 0.00986873627905356 78-79 0.005934853817472209 80-81 0.00702381782067812 82-83 0.012250845036066486 84-85 0.004138063212182458 86-87 0.0014564893542879046 88-89 0.019614964107746453 90-91 0.028476408683834546 92-93 0.013298972889152176 94-95 0.02194262466459909 96-97 0.018172086803498626 98-99 0.013149240338711362 100-101 0.10647345541345786 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3673216.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.665477483738314 #Duplication Level Percentage of deduplicated Percentage of total 1 76.03065463646713 40.8022138446721 2 13.344193981384771 14.322450832932812 3 4.465071198413476 7.188605335852404 4 2.044048116801131 4.387792727514752 5 1.0717540740753386 2.8758097065197443 6 0.6625079069717813 2.1332281898635634 7 0.4355730001598759 1.6362663122822914 8 0.30244880741744207 1.2984847731555391 9 0.22625506752275812 1.092787760855184 >10 1.2609150621811405 12.52376438297594 >50 0.1019855017450285 3.7287578592203987 >100 0.050046024752250236 4.837443240335694 >500 0.002605367739318562 0.9511756928310272 >1k 0.001787997444612579 1.604264229972944 >5k 1.5325692382393532E-4 0.6169551110157925 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9807 0.266986749486009 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7407 0.20164890929365442 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5290 0.1440154894239816 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.722410008014775E-5 0.0 4 0.0 0.0 0.0 2.722410008014775E-5 0.0 5 0.0 0.0 0.0 2.722410008014775E-5 0.0 6 0.0 0.0 0.0 2.722410008014775E-5 0.0 7 0.0 0.0 0.0 5.44482001602955E-5 0.0 8 0.0 0.0 0.0 5.44482001602955E-5 0.0 9 0.0 0.0 0.0 5.44482001602955E-5 0.0 10-11 0.0 0.0 0.0 5.44482001602955E-5 2.722410008014775E-5 12-13 0.0 0.0 0.0 6.806025020036937E-5 2.722410008014775E-5 14-15 0.0 0.0 0.0 9.528435028051713E-5 2.722410008014775E-5 16-17 0.0 0.0 0.0 1.08896400320591E-4 2.722410008014775E-5 18-19 0.0 0.0 0.0 1.08896400320591E-4 2.722410008014775E-5 20-21 0.0 0.0 0.0 1.4973255044081264E-4 2.722410008014775E-5 22-23 0.0 0.0 0.0 2.17792800641182E-4 2.722410008014775E-5 24-25 0.0 0.0 0.0 4.35585601282364E-4 2.722410008014775E-5 26-27 0.0 0.0 0.0 9.256194027250236E-4 2.722410008014775E-5 28-29 0.0 0.0 0.0 0.0028721425584555876 2.722410008014775E-5 30-31 0.0 0.0 0.0 0.009678167578492526 2.722410008014775E-5 32-33 0.0 0.0 0.0 0.024855603373174898 2.722410008014775E-5 34-35 0.0 0.0 0.0 0.04802331254138063 2.722410008014775E-5 36-37 0.0 0.0 0.0 0.08624594905390806 2.722410008014775E-5 38-39 0.0 0.0 0.0 0.14658816688155557 2.722410008014775E-5 40-41 0.0 0.0 0.0 0.21281079032651495 2.722410008014775E-5 42-43 0.0 0.0 0.0 0.2823547539812524 2.722410008014775E-5 44-45 0.0 0.0 0.0 0.3691451850367634 2.722410008014775E-5 46-47 0.0 0.0 0.0 0.4728281701920062 2.722410008014775E-5 48-49 0.0 0.0 0.0 0.5836302575182075 2.722410008014775E-5 50-51 0.0 0.0 0.0 0.703130444820016 2.722410008014775E-5 52-53 0.0 0.0 0.0 0.8207113330661742 2.722410008014775E-5 54-55 2.722410008014775E-5 0.0 0.0 0.9533335366066139 4.0836150120221626E-5 56-57 2.722410008014775E-5 0.0 0.0 1.1063329790570444 5.44482001602955E-5 58-59 2.722410008014775E-5 0.0 0.0 1.2712565773425792 5.44482001602955E-5 60-61 2.722410008014775E-5 0.0 0.0 1.4554820625849392 6.806025020036937E-5 62-63 2.722410008014775E-5 0.0 0.0 1.6385641356239327 8.167230024044325E-5 64-65 2.722410008014775E-5 0.0 0.0 1.835584947903962 8.167230024044325E-5 66-67 2.722410008014775E-5 0.0 0.0 2.0627836751228354 8.167230024044325E-5 68-69 2.722410008014775E-5 0.0 0.0 2.3130003789594733 8.167230024044325E-5 70-71 2.722410008014775E-5 0.0 0.0 2.5811986008990484 8.167230024044325E-5 72-73 2.722410008014775E-5 0.0 0.0 2.8550049874551346 8.167230024044325E-5 74-75 2.722410008014775E-5 0.0 0.0 3.134991244729414 8.167230024044325E-5 76-77 2.722410008014775E-5 0.0 0.0 3.4423513346342824 8.167230024044325E-5 78-79 2.722410008014775E-5 1.3612050040073875E-5 0.0 3.783727937589295 8.167230024044325E-5 80-81 2.722410008014775E-5 2.722410008014775E-5 0.0 4.1437938852493295 8.167230024044325E-5 82-83 2.722410008014775E-5 2.722410008014775E-5 0.0 4.514354723490261 8.167230024044325E-5 84-85 2.722410008014775E-5 2.722410008014775E-5 0.0 4.888903892392933 8.167230024044325E-5 86-87 2.722410008014775E-5 4.0836150120221626E-5 0.0 5.27529554483047 8.167230024044325E-5 88-89 2.722410008014775E-5 5.44482001602955E-5 0.0 5.692804343659616 8.167230024044325E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5755 0.0 50.508335 1 GTATCAA 10445 0.0 44.891617 1 TATCAAC 13615 0.0 34.172474 2 ATCAACG 13595 0.0 34.082916 3 TCAACGC 13660 0.0 33.920734 4 CAACGCA 13975 0.0 33.15616 5 AACGCAG 14645 0.0 31.669142 6 ACGCAGA 16895 0.0 27.331635 7 CGCAGAG 17050 0.0 27.02744 8 GTACATG 13770 0.0 25.400408 1 TGGTATC 2805 0.0 24.397255 2 TACATGG 14025 0.0 24.193945 2 GCAGAGT 18745 0.0 23.896944 9 GTGGTAT 3130 0.0 22.98853 1 ACATGGG 14555 0.0 22.431376 3 GAGTACT 10180 0.0 21.719786 12-13 TAGCCCT 2490 0.0 21.376177 4 AGAGTAC 17210 0.0 20.824038 10-11 AGTACTT 10460 0.0 20.70698 12-13 GTAAGGT 1655 0.0 20.675016 4 >>END_MODULE