FastQCFastQC Report
Thu 26 May 2016
SRR938573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938573_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3673216
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99630.271233709098512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79030.21515206293340766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55640.1514748928459421No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56150.055.9111481
GTATCAA101650.046.09281
ATCAACG136800.033.990363
TATCAAC137450.033.949122
TCAACGC138300.033.694
CAACGCA141500.032.89455
AACGCAG148600.031.321966
ACGCAGA169400.027.3639037
CGCAGAG171050.026.9611118
GCAGAGT193100.023.6856699
GTACATG138350.023.4481981
TACATGG134700.023.2713762
TGGTATC24700.022.339082
ACATGGG140100.021.7192523
GAGTACT108000.021.43840612-13
AGAGTAC179650.021.0439610-11
AGTACTT112650.020.97507512-13
GTGGTAT28800.020.4801641
CAGAGTA186350.020.15991610-11
GTACTTT115000.019.65851814-15
Produced by FastQC (version 0.11.5)