##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938573_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3673216 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.806809346360247 31.0 31.0 34.0 30.0 34.0 2 32.042642741401544 31.0 31.0 34.0 30.0 34.0 3 32.110260055493605 33.0 31.0 34.0 30.0 34.0 4 35.777782738613794 37.0 35.0 37.0 35.0 37.0 5 35.64927001298045 37.0 35.0 37.0 33.0 37.0 6 35.68411740556504 37.0 35.0 37.0 33.0 37.0 7 35.62182403648465 37.0 35.0 37.0 33.0 37.0 8 35.603251483168975 37.0 35.0 37.0 33.0 37.0 9 37.1752028739938 39.0 37.0 39.0 33.0 39.0 10-11 37.11132655961424 39.0 37.0 39.0 33.0 39.0 12-13 36.999241672692264 39.0 37.0 39.0 33.0 39.0 14-15 38.057386088920445 40.0 37.0 41.0 33.0 41.0 16-17 37.978134964020626 40.0 37.0 41.0 33.0 41.0 18-19 37.97494606905774 40.0 37.0 41.0 33.0 41.0 20-21 37.963273055545876 40.0 37.0 41.0 33.0 41.0 22-23 37.99213618257134 40.0 37.0 41.0 33.0 41.0 24-25 37.92135325012197 40.0 37.0 41.0 33.0 41.0 26-27 37.755577945865426 40.0 37.0 41.0 32.0 41.0 28-29 37.62539529393317 40.0 37.0 41.0 32.0 41.0 30-31 37.3918492677806 39.0 36.0 41.0 31.5 41.0 32-33 37.177872741488656 39.0 36.0 41.0 31.0 41.0 34-35 37.01223900255253 39.0 36.0 41.0 31.0 41.0 36-37 37.024754874202884 39.0 36.0 41.0 31.0 41.0 38-39 36.88112093054152 39.0 35.5 41.0 30.0 41.0 40-41 36.71017645028226 39.0 35.0 41.0 30.0 41.0 42-43 36.490174413919576 39.0 35.0 40.0 30.0 41.0 44-45 36.21170916711677 39.0 35.0 40.0 30.0 41.0 46-47 35.86438913475276 38.0 35.0 40.0 28.5 41.0 48-49 35.47680493061121 38.0 34.5 40.0 27.5 41.0 50-51 35.1135519936753 38.0 34.0 40.0 26.5 41.0 52-53 34.59864802396592 38.0 33.0 40.0 25.5 41.0 54-55 34.276119346098895 38.0 33.0 40.0 24.0 41.0 56-57 33.91169441165452 37.0 33.0 40.0 23.5 41.0 58-59 33.45908217757954 37.0 32.0 39.5 22.5 41.0 60-61 33.05321426782416 36.0 31.5 39.0 22.0 40.5 62-63 32.56805344417535 36.0 31.0 39.0 20.5 40.0 64-65 32.063869644475034 35.0 31.0 38.5 18.5 40.0 66-67 31.567545986949856 35.0 30.0 38.0 17.5 40.0 68-69 30.998198717418198 34.5 29.0 37.5 13.0 40.0 70-71 30.4332190102624 34.0 29.0 37.0 9.0 39.0 72-73 29.84336954320138 34.0 28.5 36.0 7.5 39.0 74-75 29.21916448692372 34.0 27.5 36.0 6.5 38.5 76-77 27.940157208288323 31.5 26.0 34.5 2.0 36.5 78-79 28.56488496728752 33.0 27.0 35.0 2.0 37.0 80-81 28.25584555876921 33.0 26.5 35.0 2.0 37.0 82-83 27.75752800815416 33.0 26.0 35.0 2.0 36.0 84-85 27.160898923450183 32.5 25.5 35.0 2.0 36.0 86-87 26.509094074511275 32.0 24.5 35.0 2.0 35.0 88-89 25.754398053367947 31.5 22.5 34.0 2.0 35.0 90-91 25.209787417892116 31.0 20.0 34.0 2.0 35.0 92-93 24.42256336137053 31.0 17.5 34.0 2.0 35.0 94-95 23.630781854375023 31.0 4.5 34.0 2.0 35.0 96-97 22.636960772249715 30.0 2.0 34.0 2.0 35.0 98-99 21.45313820368854 29.5 2.0 34.0 2.0 35.0 100-101 18.30377726221382 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25013904989107516 1101 2 0.2950226952591706 1101 3 0.2737692103988607 1101 4 0.2748042312978001 1101 5 0.224709319669941 1101 6 0.24989366779380617 1101 7 0.263369871512829 1101 8 0.2206245322704632 1101 9 0.33471448520165836 1101 10-11 0.38146637222887136 1101 12-13 0.3809695190057383 1101 14-15 0.5377901928881172 1101 16-17 0.5545364511285698 1101 18-19 0.5540540344701839 1101 20-21 0.5478483951882822 1101 22-23 0.569189915425035 1101 24-25 0.5617273139993202 1101 26-27 0.5359576594951889 1101 28-29 0.5567850868859026 1101 30-31 0.6292354157047839 1101 32-33 0.6041356865687533 1101 34-35 0.5034047824024839 1101 36-37 0.655716033421875 1101 38-39 0.7382927919845272 1101 40-41 0.8288110002882476 1101 42-43 0.6776951957858586 1101 44-45 0.7624962610208499 1101 46-47 0.906309372536434 1101 48-49 0.9101641869608343 1101 50-51 0.9569074146727559 1101 52-53 1.0453595100548156 1101 54-55 1.1145103636817097 1101 56-57 1.054300846510003 1101 58-59 1.2209603636164985 1101 60-61 1.212916786867197 1101 62-63 1.327870378335362 1101 64-65 1.3175259579824328 1101 66-67 1.4798557810654707 1101 68-69 1.543547997577516 1101 70-71 1.7137595503912273 1101 72-73 1.8412923615658556 1101 74-75 1.7233489527267594 1101 76-77 1.6412325258774239 1101 78-79 1.852181088833305 1101 80-81 2.1229536533084605 1101 82-83 2.1121665572117116 1101 84-85 2.4404516358224733 1101 86-87 2.5867646060881846 1101 88-89 2.8204721934244894 1101 90-91 3.08029005187419 1101 92-93 3.2082945466696096 1101 94-95 3.242918245406642 1101 96-97 3.639643853427188 1101 98-99 3.9253141174459287 1101 100-101 4.382262587596546 1102 1 0.3937461346586417 1102 2 0.35518502665057383 1102 3 0.33175945401239915 1102 4 0.23623559497099222 1102 5 0.25568151298700315 1102 6 0.16203361968563001 1102 7 0.31285309711932996 1102 8 0.3054266221594446 1102 9 0.44569467048416556 1102 10-11 0.3975880081462648 1102 12-13 0.3882970400308139 1102 14-15 0.6117726022460985 1102 16-17 0.59127029588781 1102 18-19 0.6074028992334561 1102 20-21 0.6141538845738879 1102 22-23 0.5498116450866632 1102 24-25 0.5960583287643288 1102 26-27 0.5159277129152571 1102 28-29 0.5094198818472364 1102 30-31 0.49185350473002387 1102 32-33 0.4492533010502484 1102 34-35 0.6248614462027575 1102 36-37 0.57848543244571 1102 38-39 0.6659501163363828 1102 40-41 0.6303157156975843 1102 42-43 0.6623247613666337 1102 44-45 0.657339280619631 1102 46-47 0.636692748982135 1102 48-49 0.7742720771634808 1102 50-51 0.7082468258259382 1102 52-53 1.015775429970141 1102 54-55 0.8947137628622457 1102 56-57 1.0746573174483771 1102 58-59 1.1299155488865225 1102 60-61 1.2186660028510587 1102 62-63 1.2745817361260379 1102 64-65 1.2699862406258404 1102 66-67 1.2630729697136225 1102 68-69 1.4477263448410618 1102 70-71 1.639102382252016 1102 72-73 1.58123274123745 1102 74-75 1.6826287741618948 1102 76-77 1.6665120658303465 1102 78-79 2.0321591442004596 1102 80-81 2.197102880804483 1102 82-83 2.345626940170593 1102 84-85 2.5148928262526766 1102 86-87 2.5082016226961343 1102 88-89 2.717107208314598 1102 90-91 3.0058661629511967 1102 92-93 3.1888590066787543 1102 94-95 3.231623480460101 1102 96-97 3.774269002132808 1102 98-99 4.033139564203289 1102 100-101 4.435664313455163 1103 1 0.23301282649742916 1103 2 0.27435423171871065 1103 3 0.3194087255323552 1103 4 0.1897002071197207 1103 5 0.1465440833157956 1103 6 0.14641571383605623 1103 7 0.1807269218142622 1103 8 0.19171095414925787 1103 9 0.31971159465324916 1103 10-11 0.3810571333640951 1103 12-13 0.3685150203092533 1103 14-15 0.5349731038972365 1103 16-17 0.5063853351708616 1103 18-19 0.5074022755279444 1103 20-21 0.5390591413078667 1103 22-23 0.4597551261726309 1103 24-25 0.43306160929539317 1103 26-27 0.3847863926010575 1103 28-29 0.39583783074848355 1103 30-31 0.42843109025724146 1103 32-33 0.518500645224222 1103 34-35 0.5359255981527724 1103 36-37 0.6768610634077916 1103 38-39 0.7760654043020168 1103 40-41 0.7693387945606673 1103 42-43 0.8497188813589389 1103 44-45 0.9154171865555654 1103 46-47 0.9879121172452159 1103 48-49 0.9721650400221264 1103 50-51 0.960357309340047 1103 52-53 1.1420772353097348 1103 54-55 1.1438476518052383 1103 56-57 1.1069935695635778 1103 58-59 1.3720803683453724 1103 60-61 1.3173340157620785 1103 62-63 1.3286166230138505 1103 64-65 1.2775835334880412 1103 66-67 1.2815586415188172 1103 68-69 1.4118785507355263 1103 70-71 1.3781735024319133 1103 72-73 1.5345479039502194 1103 74-75 1.6686008375924253 1103 76-77 1.6036948855901692 1103 78-79 1.8833776728599645 1103 80-81 2.130127903709667 1103 82-83 2.2616397955407983 1103 84-85 2.3877926378776166 1103 86-87 2.586984984344099 1103 88-89 2.962372243975363 1103 90-91 3.0008484121324237 1103 92-93 3.133234350416455 1103 94-95 3.177507231987974 1103 96-97 3.550826142913216 1103 98-99 3.972308646129566 1103 100-101 4.349783223070798 1104 1 0.2277571802370062 1104 2 0.25146435557353186 1104 3 0.2519441825331086 1104 4 0.1512919751792836 1104 5 0.0458281734133692 1104 6 0.1272157674493215 1104 7 0.14943086213129675 1104 8 0.15505394727035338 1104 9 0.1485589868365409 1104 10-11 0.22924312953747972 1104 12-13 0.25329498684038043 1104 14-15 0.3715221716314403 1104 16-17 0.289198933713962 1104 18-19 0.3773125218320601 1104 20-21 0.42586120420551055 1104 22-23 0.41011509768603815 1104 24-25 0.2994288468007795 1104 26-27 0.3438565481932372 1104 28-29 0.432833256184729 1104 30-31 0.383211578365362 1104 32-33 0.40634802270025006 1104 34-35 0.42624296107165804 1104 36-37 0.5892330590902262 1104 38-39 0.6473911104816281 1104 40-41 0.6066737908755115 1104 42-43 0.6484846998110285 1104 44-45 0.7236545550298459 1104 46-47 0.7350489779855209 1104 48-49 0.8993940576546251 1104 50-51 0.9000469755807998 1104 52-53 1.0574065270278865 1104 54-55 1.0547685187207847 1104 56-57 1.1925608141041906 1104 58-59 1.3386946271382243 1104 60-61 1.3824526752849593 1104 62-63 1.3245735295346677 1104 64-65 1.2869254473693132 1104 66-67 1.4350741007423373 1104 68-69 1.369862529831206 1104 70-71 1.5371030899933977 1104 72-73 1.7502063804935872 1104 74-75 1.7910381547277616 1104 76-77 1.775092134241234 1104 78-79 2.0580323517466965 1104 80-81 2.3340388820410247 1104 82-83 2.4292974584847116 1104 84-85 2.5031304052218353 1104 86-87 2.5674356268617515 1104 88-89 2.5868781672856507 1104 90-91 2.93079467150811 1104 92-93 3.1812127619810333 1104 94-95 3.35442902712861 1104 96-97 3.550987164646145 1104 98-99 3.9955809109851472 1104 100-101 4.345866695402886 1105 1 0.24469872355058442 1105 2 0.27018636215125014 1105 3 0.17512585305817652 1105 4 0.17956296797215998 1105 5 0.11977517064046594 1105 6 0.14018813521740725 1105 7 0.11277120764599857 1105 8 0.17048228015478628 1105 9 0.2969449220755038 1105 10-11 0.3229970346140476 1105 12-13 0.3321712781849371 1105 14-15 0.4556309600004127 1105 16-17 0.4729503208474881 1105 18-19 0.4188235306788002 1105 20-21 0.3532315973655571 1105 22-23 0.3643771872278876 1105 24-25 0.2804796717183251 1105 26-27 0.4631091922131745 1105 28-29 0.5185536143797407 1105 30-31 0.5212924973757467 1105 32-33 0.5589526988842977 1105 34-35 0.5932178157219212 1105 36-37 0.6434772958263366 1105 38-39 0.5941725292986746 1105 40-41 0.6081874327055274 1105 42-43 0.6799860941705163 1105 44-45 0.6955018379737368 1105 46-47 0.7898385773133398 1105 48-49 0.8839611231609652 1105 50-51 1.0324626306004632 1105 52-53 1.031389639697288 1105 54-55 1.0162739006622488 1105 56-57 1.0635819140642297 1105 58-59 1.354105955638623 1105 60-61 1.346568176251374 1105 62-63 1.5537271613044084 1105 64-65 1.4519960986077436 1105 66-67 1.4895487055396082 1105 68-69 1.56185227140627 1105 70-71 1.6312195155956566 1105 72-73 1.7008650864721844 1105 74-75 1.9297635147281866 1105 76-77 1.66203965533278 1105 78-79 1.9327724507014246 1105 80-81 2.196440628696841 1105 82-83 2.2015252801303653 1105 84-85 2.427074253222628 1105 86-87 2.4927678306917542 1105 88-89 2.748677541725641 1105 90-91 2.94682592304947 1105 92-93 3.2579814803566514 1105 94-95 3.3244884410935427 1105 96-97 3.5774767594730186 1105 98-99 4.084196831776499 1105 100-101 4.6385417675798415 1106 1 0.15783773387732225 1106 2 0.16716489009596813 1106 3 0.16470941253136573 1106 4 0.076620165718019 1106 5 0.06332865689843459 1106 6 0.06508498499341187 1106 7 0.12111350238678398 1106 8 0.08663589146765105 1106 9 0.11074790409085722 1106 10-11 0.18167716169801906 1106 12-13 0.09784550166635597 1106 14-15 0.05025862220833943 1106 16-17 0.07575437622558212 1106 18-19 0.26464275736324794 1106 20-21 0.4098039254707899 1106 22-23 0.28810860623406853 1106 24-25 0.17171551912498728 1106 26-27 0.10083866281628673 1106 28-29 0.2534079760147918 1106 30-31 0.2698321802361434 1106 32-33 0.2772329526710706 1106 34-35 0.11466135498599073 1106 36-37 0.2471298458491944 1106 38-39 0.3495078715062192 1106 40-41 0.3062991282038112 1106 42-43 0.49018097675011063 1106 44-45 0.02159131620309296 1106 46-47 -0.18411956906278704 1106 48-49 0.33198159249869974 1106 50-51 0.19958630576125103 1106 52-53 0.3756130444503327 1106 54-55 0.5289289197561615 1106 56-57 0.3760514859392998 1106 58-59 0.7974851216541836 1106 60-61 0.5855040890944068 1106 62-63 0.6716498257373615 1106 64-65 0.5187279365413602 1106 66-67 0.6676427698314562 1106 68-69 0.39526087124788134 1106 70-71 0.6827217971273605 1106 72-73 0.9781604086783986 1106 74-75 1.3175346667127243 1106 76-77 1.361527678285448 1106 78-79 1.6741507456173146 1106 80-81 1.6181530710853629 1106 82-83 2.010805608359693 1106 84-85 1.9259034846566472 1106 86-87 2.2118679556391747 1106 88-89 2.4965925591386764 1106 90-91 2.526585888446217 1106 92-93 2.145475819247668 1106 94-95 2.5528828513534023 1106 96-97 2.9883170066853495 1106 98-99 3.2819868265397254 1106 100-101 3.373479850219102 1107 1 0.1746426443034963 1107 2 0.1994937703659545 1107 3 0.1653049596359537 1107 4 0.1820655206850148 1107 5 0.07813700457815287 1107 6 0.04064880072409238 1107 7 0.12717659315379848 1107 8 0.1868616899580502 1107 9 0.26260548835279707 1107 10-11 0.2575045617769973 1107 12-13 0.25895635270813955 1107 14-15 0.43028848843029976 1107 16-17 0.4229865376445332 1107 18-19 0.36852405770896723 1107 20-21 0.377850699604636 1107 22-23 0.3143015879867832 1107 24-25 0.3075694630693846 1107 26-27 0.34443733614082106 1107 28-29 0.2875538312455532 1107 30-31 0.2565832339192795 1107 32-33 0.4033649452502033 1107 34-35 0.4403903543933865 1107 36-37 0.5336153629147233 1107 38-39 0.36194998479647467 1107 40-41 0.3557474161930685 1107 42-43 0.608721545571413 1107 44-45 0.6940703924592171 1107 46-47 0.7851503472856223 1107 48-49 0.7790826159986324 1107 50-51 0.7635058076092847 1107 52-53 0.8275611538314323 1107 54-55 0.910604465049822 1107 56-57 1.0387223331213278 1107 58-59 1.021962576477172 1107 60-61 1.0989327281576635 1107 62-63 1.0856150309800014 1107 64-65 1.2354405725031015 1107 66-67 1.400498398955147 1107 68-69 1.4722384658435494 1107 70-71 1.5034020811174713 1107 72-73 1.6671394844796978 1107 74-75 1.6238342129815422 1107 76-77 1.5997856704667122 1107 78-79 1.8450916575622003 1107 80-81 2.045426665397219 1107 82-83 2.1097489268438814 1107 84-85 2.374729537529838 1107 86-87 2.6810968323474853 1107 88-89 3.0054880498233807 1107 90-91 3.0055429791758748 1107 92-93 3.1852511025523853 1107 94-95 3.435880551804054 1107 96-97 3.6824804771232635 1107 98-99 3.7400011405224305 1107 100-101 4.019393649061609 1108 1 0.15199064514403915 1108 2 0.11277768705581792 1108 3 0.14046564253637683 1108 4 0.11923814403142075 1108 5 0.033499638885650995 1108 6 0.044259782638988554 1108 7 0.13412636742521045 1108 8 0.1551016718756557 1108 9 0.16843862979580138 1108 10-11 0.15209720162563656 1108 12-13 0.14902041394456234 1108 14-15 0.3047198622570946 1108 16-17 0.3607037718992743 1108 18-19 0.367185560588716 1108 20-21 0.4141834691545867 1108 22-23 0.3835061474466599 1108 24-25 0.25568907362507076 1108 26-27 0.33888960299969995 1108 28-29 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56-57 -0.01638121309437679 2316 58-59 -0.07515377712454807 2316 60-61 0.24450638307268235 2316 62-63 0.21174016771186643 2316 64-65 0.4235632322523273 2316 66-67 0.46021554395348474 2316 68-69 0.5349188409025842 2316 70-71 0.5306631854938324 2316 72-73 0.6399803655912564 2316 74-75 0.9475492361973679 2316 76-77 0.896504913029375 2316 78-79 0.9553178890907894 2316 80-81 1.0701555898539574 2316 82-83 1.3977755883798793 2316 84-85 1.479616364583709 2316 86-87 1.6450684741927546 2316 88-89 1.82303634829373 2316 90-91 2.1540102032196913 2316 92-93 2.187452166000007 2316 94-95 2.6504905447673224 2316 96-97 2.891208948161676 2316 98-99 3.1102596898797756 2316 100-101 3.494312921762461 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 21.0 4 45.0 5 149.0 6 276.0 7 681.0 8 1538.0 9 2736.0 10 4538.0 11 6804.0 12 9921.0 13 13938.0 14 18487.0 15 24457.0 16 30515.0 17 37901.0 18 37426.0 19 33143.0 20 33741.0 21 36087.0 22 39650.0 23 45364.0 24 52069.0 25 60701.0 26 71675.0 27 85784.0 28 102581.0 29 120873.0 30 141949.0 31 168882.0 32 201716.0 33 245078.0 34 303416.0 35 383384.0 36 486637.0 37 550412.0 38 303436.0 39 17197.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.098223571087104 19.264719814701685 15.349127684435313 23.2879289297759 2 16.06124165986437 20.349726668796556 39.30887011572487 24.280161555614203 3 19.47683891659854 23.8345816968215 29.489800335347105 27.198779051232858 4 13.522618871310591 16.960587125831967 36.13476582918075 33.382028173676694 5 14.139344932421016 37.16323602991592 33.917302814237644 14.780116223425418 6 33.01945760880928 35.84983839774193 17.026006638324564 14.10469735512423 7 29.493092701327665 31.965857711607487 20.020957112241696 18.52009247482315 8 26.64063316723003 35.16964425723943 19.246077551660452 18.94364502387009 9 26.305205030142524 16.803966878070877 19.692335000174236 37.19849309161236 10-11 25.2281243466216 26.513414947555493 27.051594569989895 21.206866135833014 12-13 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28.137364829573535 22.752001071576025 38-39 22.528943187206448 26.464666651510015 28.169272362399294 22.837117798884243 40-41 23.03459906099912 26.19903393637778 28.15031474049513 22.616052262127965 42-43 22.691066306743387 26.27456265373992 28.013316072262356 23.021054967254333 44-45 22.68542989428703 26.519405304474564 28.165001477632078 22.630163323606325 46-47 22.99228332673149 26.680020091391327 27.467436564426528 22.860260017450653 48-49 22.862676738857722 26.665461546503117 27.569560298114787 22.902301416524377 50-51 23.042723324737775 26.786241266508693 27.45919379638987 22.711841612363664 52-53 23.179020781789035 26.647030830748857 27.36957205892602 22.804376328536083 54-55 22.805696697389973 26.734746881207094 27.54398325608949 22.915573165313447 56-57 22.885966956476285 26.889679234763214 27.358042652542075 22.86631115621842 58-59 22.959894000243928 26.569632714220997 27.320691187232114 23.14978209830296 60-61 22.69308420740844 26.769253428058683 27.5430712487368 22.994591115796077 62-63 22.679431321218246 26.781871798445835 27.656650194271176 22.882046686064744 64-65 22.50670801825975 26.67671871188626 27.50335128671987 23.313221983134124 66-67 22.471324792933046 26.793745412729148 27.392431901487747 23.342497892850066 68-69 22.76295308664158 26.564644115531184 27.5333206740975 23.139082123729736 70-71 22.626140145311357 26.618486361814824 27.272395633689932 23.482977859183887 72-73 22.675762240979633 26.68270166597926 27.206820183408816 23.4347159096323 74-75 22.65453488169495 26.95232188904764 26.94542057967732 23.447722649580097 76-77 22.61183388071924 26.6916783548803 27.32380834669129 23.37267941770917 78-79 22.629447873471094 26.792162508276125 27.29217394239816 23.286215675854617 80-81 22.64452566001795 26.99462555208796 27.08147046680775 23.27937832108634 82-83 22.683873939592594 26.97732520539789 27.086971477404227 23.25182937760529 84-85 22.672261636820078 26.893849571243166 27.100421147375446 23.333467644561306 86-87 22.622895835100092 26.960644120875166 26.991435034914744 23.42502500911 88-89 22.585612683386717 27.055008630723854 26.778016911345638 23.581361774543797 90-91 22.58567463736167 26.947666255261616 26.953070285470275 23.513588821906442 92-93 22.445268217145856 26.74422102638606 27.05822443784501 23.752286318623078 94-95 22.28815294281632 26.77502493727567 27.049253297383004 23.887568822525 96-97 22.50153543924452 26.571170875875527 26.963810459281458 23.963483225598495 98-99 22.218867608112348 26.68921457382305 26.82042112415932 24.271496693905288 100-101 22.36922434848515 26.507031559357337 27.02082209480344 24.10292199735407 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 477.0 1 355.0 2 276.5 3 339.5 4 306.5 5 309.0 6 563.0 7 1331.0 8 2174.5 9 2501.0 10 2277.5 11 1997.5 12 2031.0 13 2238.0 14 2495.5 15 2715.5 16 2954.5 17 3250.0 18 3612.0 19 4050.0 20 4560.0 21 5305.0 22 6380.0 23 7774.0 24 9648.0 25 12176.0 26 15576.0 27 19816.0 28 24048.0 29 29019.5 30 35198.5 31 43244.0 32 53967.5 33 60372.0 34 64388.0 35 71222.0 36 79340.5 37 87850.5 38 94147.5 39 99095.5 40 104739.0 41 112942.0 42 119735.0 43 125355.0 44 131893.5 45 137949.5 46 141542.5 47 144974.5 48 146207.5 49 143863.5 50 142141.5 51 137549.5 52 133875.0 53 134034.0 54 134596.0 55 125801.0 56 112068.0 57 101283.5 58 92563.5 59 81394.0 60 65955.5 61 54410.0 62 45514.5 63 35108.5 64 26225.5 65 20309.5 66 15310.5 67 11881.0 68 8993.5 69 6589.0 70 5013.0 71 3649.5 72 2792.5 73 2337.5 74 1761.5 75 1281.0 76 920.0 77 567.5 78 354.0 79 243.5 80 152.0 81 89.5 82 50.0 83 20.0 84 11.0 85 7.0 86 5.5 87 5.0 88 3.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0889640032059099E-4 2 0.04527367843328571 3 1.9056870056103424E-4 4 0.0 5 0.1016003414991114 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011570242534062795 26-27 0.011719975084503606 28-29 0.0 30-31 0.0 32-33 4.083615012022163E-4 34-35 0.01587165034672614 36-37 0.003307728159737952 38-39 0.012536698086908039 40-41 0.007336894971599819 42-43 0.005744285116911175 44-45 0.0029810389587761788 46-47 2.7224100080147747E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.1779280064118198E-4 68-69 2.314048506812559E-4 70-71 0.0 72-73 2.7224100080147747E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.0836150120221626E-5 82-83 0.0011025760532459839 84-85 0.004219735512422901 86-87 0.0014837134543680523 88-89 4.0836150120221626E-5 90-91 8.575591525246542E-4 92-93 2.0418075060110814E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.214386521239154E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3673216.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.93912336453788 #Duplication Level Percentage of deduplicated Percentage of total 1 73.99412333849556 37.69195776990456 2 14.316470303525891 14.585368938720967 3 4.847580070297537 7.407944376610843 4 2.239218918016873 4.56255394820274 5 1.1883325054819993 3.026630804741897 6 0.7290342356641647 2.2281818920481076 7 0.4927743409478755 1.7571045061095871 8 0.3634940333917382 1.481285392737216 9 0.25735580794287527 1.1798531324444195 >10 1.3896057479540982 13.15278719351517 >50 0.1198420313874802 4.175438900727843 >100 0.05665769404018819 5.233855935680679 >500 0.0035313030077274905 1.2341504750260535 >1k 0.001819156074710018 1.6443074882175897 >5k 1.6051377129794276E-4 0.638579245312329 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9963 0.271233709098512 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7903 0.21515206293340766 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5564 0.1514748928459421 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.722410008014775E-5 0.0 4 0.0 0.0 0.0 2.722410008014775E-5 0.0 5 0.0 0.0 0.0 2.722410008014775E-5 0.0 6 0.0 0.0 0.0 2.722410008014775E-5 0.0 7 0.0 0.0 0.0 5.44482001602955E-5 0.0 8 0.0 0.0 0.0 5.44482001602955E-5 0.0 9 0.0 0.0 0.0 5.44482001602955E-5 0.0 10-11 0.0 0.0 0.0 5.44482001602955E-5 0.0 12-13 0.0 0.0 0.0 6.806025020036937E-5 0.0 14-15 0.0 0.0 0.0 9.528435028051713E-5 0.0 16-17 0.0 0.0 0.0 1.08896400320591E-4 0.0 18-19 0.0 0.0 0.0 1.08896400320591E-4 0.0 20-21 0.0 0.0 0.0 1.4973255044081264E-4 0.0 22-23 0.0 0.0 0.0 2.3140485068125585E-4 0.0 24-25 0.0 0.0 0.0 4.6280970136251175E-4 0.0 26-27 0.0 0.0 0.0 9.93679652925393E-4 0.0 28-29 0.0 0.0 0.0 0.0029810389587761788 0.0 30-31 0.0 0.0 0.0 0.009787063978813116 0.0 32-33 0.0 0.0 0.0 0.025087008223856153 0.0 34-35 0.0 0.0 0.0 0.048513346342823296 0.0 36-37 0.0 0.0 0.0 0.08718518050667318 0.0 38-39 0.0 0.0 0.0 0.14785408753528245 0.0 40-41 0.0 0.0 0.0 0.21415838328048228 0.0 42-43 0.0 0.0 0.0 0.28445100968742376 0.0 44-45 0.0 0.0 0.0 0.371840370944698 0.0 46-47 0.0 0.0 0.0 0.47635369115238524 0.0 48-49 0.0 0.0 0.0 0.5873599592291877 0.0 50-51 0.0 0.0 0.0 0.7071868357319581 0.0 52-53 0.0 0.0 0.0 0.8252713698295989 0.0 54-55 0.0 0.0 0.0 0.9585469517719623 0.0 56-57 0.0 0.0 0.0 1.112349505174757 0.0 58-59 0.0 0.0 0.0 1.277994542112416 0.0 60-61 0.0 0.0 0.0 1.4631456467575008 0.0 62-63 0.0 0.0 0.0 1.6467722017980972 0.0 64-65 0.0 0.0 0.0 1.8443238840296896 0.0 66-67 0.0 0.0 0.0 2.0729791006028506 0.0 68-69 0.0 0.0 0.0 2.3256323613966616 0.0 70-71 0.0 0.0 0.0 2.5975602850472175 0.0 72-73 0.0 0.0 0.0 2.875913096316688 0.0 74-75 0.0 0.0 0.0 3.1625148099104434 0.0 76-77 0.0 0.0 0.0 3.478436879290518 0.0 78-79 0.0 0.0 0.0 3.8288246593720596 0.0 80-81 0.0 0.0 0.0 4.2003927893159565 0.0 82-83 0.0 0.0 0.0 4.584538453496881 0.0 84-85 0.0 0.0 0.0 4.9738703087430745 0.0 86-87 0.0 0.0 0.0 5.3777262213820265 0.0 88-89 0.0 0.0 0.0 5.819355572882182 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5615 0.0 55.911148 1 GTATCAA 10165 0.0 46.0928 1 ATCAACG 13680 0.0 33.99036 3 TATCAAC 13745 0.0 33.94912 2 TCAACGC 13830 0.0 33.69 4 CAACGCA 14150 0.0 32.8945 5 AACGCAG 14860 0.0 31.32196 6 ACGCAGA 16940 0.0 27.363903 7 CGCAGAG 17105 0.0 26.961111 8 GCAGAGT 19310 0.0 23.685669 9 GTACATG 13835 0.0 23.448198 1 TACATGG 13470 0.0 23.271376 2 TGGTATC 2470 0.0 22.33908 2 ACATGGG 14010 0.0 21.719252 3 GAGTACT 10800 0.0 21.438406 12-13 AGAGTAC 17965 0.0 21.04396 10-11 AGTACTT 11265 0.0 20.975075 12-13 GTGGTAT 2880 0.0 20.480164 1 CAGAGTA 18635 0.0 20.159916 10-11 GTACTTT 11500 0.0 19.658518 14-15 >>END_MODULE