FastQCFastQC Report
Thu 26 May 2016
SRR938572_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938572_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3660580
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90720.24782957891918764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70000.19122652694381764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47500.12976085756901912No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54750.054.655971
GTATCAA102550.045.7479931
ATCAACG134300.034.648743
TATCAAC136350.034.2672422
TCAACGC136750.034.0627374
CAACGCA140250.033.144455
AACGCAG146550.031.7113746
ACGCAGA168300.027.5209717
CGCAGAG168700.027.314928
GTACATG136000.025.442121
TACATGG134050.024.9978732
GCAGAGT187350.023.9599429
ACATGGG141600.022.7922553
TGGTATC24750.022.6614442
GAGTACT102700.022.17772312-13
GTGGTAT29600.020.9607011
AGTACTT107750.020.91788112-13
GTAAGGT15950.020.8601864
AGAGTAC170400.020.80894710-11
CAGAGTA178050.020.40841710-11
Produced by FastQC (version 0.11.5)