##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938572_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3660580 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.96685388654257 31.0 31.0 34.0 28.0 34.0 2 31.142569210343716 31.0 31.0 34.0 27.0 34.0 3 31.19260499702233 31.0 31.0 34.0 27.0 34.0 4 34.85856093843052 37.0 35.0 37.0 32.0 37.0 5 34.84857317692825 37.0 35.0 37.0 32.0 37.0 6 34.81762043173486 37.0 35.0 37.0 32.0 37.0 7 34.727074671226966 37.0 35.0 37.0 31.0 37.0 8 34.67562654005649 37.0 35.0 37.0 30.0 37.0 9 36.13566647908255 39.0 35.0 39.0 31.0 39.0 10-11 35.95955599932252 38.0 35.0 39.0 30.0 39.0 12-13 35.76328901430921 38.0 35.0 39.0 30.0 39.0 14-15 36.89602412732408 39.0 36.0 41.0 31.0 41.0 16-17 36.81937643761371 39.0 36.0 41.0 30.0 41.0 18-19 36.804290850083866 39.0 36.0 41.0 30.0 41.0 20-21 36.72510722344546 39.0 36.0 41.0 30.0 41.0 22-23 36.55591463647838 39.0 36.0 41.0 30.0 41.0 24-25 36.40991632473542 39.0 36.0 41.0 29.0 41.0 26-27 36.18770058296773 39.0 35.0 40.5 28.5 41.0 28-29 35.93702910467741 39.0 35.0 40.0 27.0 41.0 30-31 35.65563489938753 38.0 34.5 40.0 27.0 41.0 32-33 35.34541793923367 38.0 34.0 40.0 26.5 41.0 34-35 35.02618847286496 38.0 34.0 40.0 25.0 41.0 36-37 34.78771683722252 38.0 33.5 40.0 25.0 41.0 38-39 34.53154363516164 38.0 33.0 40.0 24.0 41.0 40-41 34.23590400974709 38.0 33.0 40.0 24.0 41.0 42-43 33.80283302099667 38.0 33.0 40.0 22.0 41.0 44-45 33.31556925405263 37.5 32.0 40.0 19.0 41.0 46-47 32.81094512345038 37.0 31.0 40.0 16.5 41.0 48-49 32.301118128821116 37.0 30.5 39.5 13.0 41.0 50-51 31.57520474897421 35.5 30.0 38.5 9.0 40.0 52-53 31.56171030273891 36.0 30.0 39.0 8.5 40.0 54-55 31.499031710821782 36.0 30.0 39.0 7.5 40.0 56-57 31.037825016800618 36.0 29.0 39.0 2.0 40.0 58-59 30.544662867632997 35.0 28.0 39.0 2.0 40.0 60-61 29.95362961607177 35.0 28.0 38.5 2.0 40.0 62-63 29.194167181157084 34.0 26.5 38.0 2.0 40.0 64-65 28.501139027148703 34.0 26.0 38.0 2.0 40.0 66-67 27.768853433062517 33.5 24.0 37.0 2.0 39.5 68-69 26.882041507083578 32.5 21.5 36.5 2.0 39.0 70-71 26.00522021100481 32.0 19.0 36.0 2.0 39.0 72-73 25.074603478137345 31.0 14.5 35.0 2.0 38.0 74-75 24.10339959787793 30.5 7.5 35.0 2.0 37.5 76-77 23.119229466368715 29.5 2.0 34.0 2.0 36.5 78-79 22.109084625933598 29.0 2.0 34.0 2.0 36.0 80-81 21.118306661785837 28.0 2.0 34.0 2.0 35.5 82-83 20.088353758147615 26.0 2.0 33.0 2.0 35.0 84-85 19.09093873102077 25.5 2.0 32.5 2.0 35.0 86-87 18.07786785154265 23.5 2.0 32.0 2.0 34.5 88-89 16.970562178671138 19.0 2.0 31.5 2.0 34.0 90-91 15.809456015166996 10.0 2.0 31.0 2.0 34.0 92-93 14.65195378874386 2.0 2.0 30.5 2.0 34.0 94-95 13.420031388468495 2.0 2.0 29.5 2.0 34.0 96-97 12.044376301023334 2.0 2.0 29.0 2.0 34.0 98-99 10.41718252298816 2.0 2.0 26.0 2.0 33.0 100-101 7.369226188199685 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7152031731625996 1101 2 0.5846186423039406 1101 3 0.511263880858472 1101 4 0.41821004835635023 1101 5 0.5136661582776654 1101 6 0.5981951366003528 1101 7 0.5473023696145987 1101 8 0.4769086771559543 1101 9 0.6318054630911263 1101 10-11 0.796554178973274 1101 12-13 0.6755098884147586 1101 14-15 0.8676758966313258 1101 16-17 0.8151133999470801 1101 18-19 0.7069608430352261 1101 20-21 0.9286569531962456 1101 22-23 0.9906623330860782 1101 24-25 1.003548774308733 1101 26-27 0.9863122767834156 1101 28-29 1.0998398511479834 1101 30-31 1.071390227860178 1101 32-33 1.1613212023333688 1101 34-35 1.2536304042644133 1101 36-37 1.3798092229428462 1101 38-39 1.3942794876919464 1101 40-41 1.4149409194501956 1101 42-43 1.53511195165796 1101 44-45 1.7011566909314482 1101 46-47 1.5981140944023124 1101 48-49 1.617178458491395 1101 50-51 1.6512620643759313 1101 52-53 1.5946672901668784 1101 54-55 1.8494800807308103 1101 56-57 2.116435930358147 1101 58-59 1.9827359399877196 1101 60-61 2.1086039898459497 1101 62-63 2.110805127357846 1101 64-65 2.142471485451072 1101 66-67 2.3978011480249783 1101 68-69 2.4419637382672725 1101 70-71 2.5419871191495176 1101 72-73 2.7292944219165562 1101 74-75 2.9466858001593472 1101 76-77 3.3816158324609304 1101 78-79 3.650126012204126 1101 80-81 3.8554986534458315 1101 82-83 4.030592276371781 1101 84-85 3.9632454218758966 1101 86-87 4.088439899047248 1101 88-89 4.267540555234415 1101 90-91 4.423295530635622 1101 92-93 4.307349183435983 1101 94-95 4.185393832218969 1101 96-97 3.8997777991744336 1101 98-99 3.5634312608943386 1101 100-101 2.5211950224031314 1102 1 0.7201946474382801 1102 2 0.531806777066965 1102 3 0.5493324201154763 1102 4 0.4305726834195269 1102 5 0.4277287655188218 1102 6 0.5712141402774833 1102 7 0.5813606545663035 1102 8 0.6410143064106535 1102 9 0.7004621517754686 1102 10-11 0.7420561688669522 1102 12-13 0.8348432966320658 1102 14-15 0.9789045417258251 1102 16-17 0.9368715991560777 1102 18-19 0.9855428609049284 1102 20-21 0.8941816727747423 1102 22-23 0.8784399966592815 1102 24-25 0.8806851018626105 1102 26-27 0.9218766000504957 1102 28-29 0.9223904505601368 1102 30-31 1.0243705987735439 1102 32-33 1.1080790332605375 1102 34-35 1.108831363939899 1102 36-37 1.274399388880596 1102 38-39 1.3086794076074 1102 40-41 1.4543520552213067 1102 42-43 1.729436695208257 1102 44-45 1.656349347525719 1102 46-47 1.6990239430284362 1102 48-49 1.763431877234865 1102 50-51 1.8520355064349907 1102 52-53 1.7311077977529798 1102 54-55 1.7924488775507292 1102 56-57 2.016328057262708 1102 58-59 2.2168973103710954 1102 60-61 2.2625924569331097 1102 62-63 2.4025932293772705 1102 64-65 2.589399730424109 1102 66-67 2.7790532761080406 1102 68-69 2.7422442936898364 1102 70-71 2.6209556571677552 1102 72-73 2.8289086299616386 1102 74-75 2.9679877651437785 1102 76-77 3.2651834488371527 1102 78-79 3.5456686470195464 1102 80-81 3.9020539126275153 1102 82-83 3.9393773213172274 1102 84-85 4.055981255329478 1102 86-87 4.01184527221216 1102 88-89 4.187231764313701 1102 90-91 4.167131204288372 1102 92-93 4.091337704091403 1102 94-95 3.972069345461543 1102 96-97 3.8188560282611306 1102 98-99 3.5797628022575765 1102 100-101 2.5664096101802594 1103 1 0.5245936841880869 1103 2 0.5132493219118288 1103 3 0.5431664536609233 1103 4 0.45972394865641775 1103 5 0.4826000408543081 1103 6 0.4141004599237732 1103 7 0.5956346241540089 1103 8 0.4803343993694753 1103 9 0.5724217315187303 1103 10-11 0.6820640664827806 1103 12-13 0.6359458879563462 1103 14-15 0.7453173410422238 1103 16-17 0.7741044881945811 1103 18-19 0.8273006954045599 1103 20-21 0.8047131758463948 1103 22-23 1.043861871760427 1103 24-25 0.9787636184055302 1103 26-27 0.9868058451808395 1103 28-29 0.9219405453962608 1103 30-31 0.9366322946894456 1103 32-33 1.1168377805174146 1103 34-35 1.1070974488586955 1103 36-37 1.2015998782738393 1103 38-39 1.4095025074863372 1103 40-41 1.4452599250012739 1103 42-43 1.5171643180906287 1103 44-45 1.4167159393160844 1103 46-47 1.4997107766671007 1103 48-49 1.3012209979453928 1103 50-51 1.618058441888163 1103 52-53 1.7405511129390554 1103 54-55 1.7646021754526764 1103 56-57 1.9034816464701656 1103 58-59 2.0663119481488934 1103 60-61 2.2425389861898886 1103 62-63 2.391560842678924 1103 64-65 2.4654770752715613 1103 66-67 2.592096641787073 1103 68-69 2.597669858660673 1103 70-71 2.411002395517155 1103 72-73 2.896214927986591 1103 74-75 2.929209338581895 1103 76-77 3.069326136745385 1103 78-79 3.265654631988184 1103 80-81 3.4184458350496243 1103 82-83 3.683835881491234 1103 84-85 3.73244878771294 1103 86-87 3.909363349336907 1103 88-89 4.101180766430524 1103 90-91 3.905875645208381 1103 92-93 3.905050101406216 1103 94-95 3.837182760403577 1103 96-97 3.706627346968771 1103 98-99 3.2880494727651595 1103 100-101 2.175986764060771 1104 1 0.6371570135187561 1104 2 0.5566582320272815 1104 3 0.5413085412376368 1104 4 0.4890617242429798 1104 5 0.5051183628803599 1104 6 0.5367515655971715 1104 7 0.625398275311106 1104 8 0.5863719719997107 1104 9 0.6690829335888253 1104 10-11 0.7085923639181431 1104 12-13 0.7458819763739157 1104 14-15 0.8409515115073916 1104 16-17 0.9499546513763022 1104 18-19 0.8666048818703089 1104 20-21 0.8669057544806336 1104 22-23 0.8583523031711735 1104 24-25 1.0182187366253217 1104 26-27 1.0129514444161956 1104 28-29 1.0741192256054504 1104 30-31 1.0350742797293577 1104 32-33 0.9981144544534004 1104 34-35 1.0517620567234758 1104 36-37 1.2651432809833523 1104 38-39 1.1831868453677927 1104 40-41 1.1503073671768718 1104 42-43 1.244866495560963 1104 44-45 1.3507465128264968 1104 46-47 1.5161070617496577 1104 48-49 1.567203581812329 1104 50-51 1.66680402962972 1104 52-53 1.6679908990333736 1104 54-55 2.0113675022849584 1104 56-57 2.329224357736816 1104 58-59 2.4481081116391294 1104 60-61 2.5081140694443285 1104 62-63 2.523431035321078 1104 64-65 2.6164672057262024 1104 66-67 2.7336254080485354 1104 68-69 3.1542161832153646 1104 70-71 3.2398708156942178 1104 72-73 3.4896475062578602 1104 74-75 3.3661945750970936 1104 76-77 3.4007137409835764 1104 78-79 3.7550327719887306 1104 80-81 3.932120206595812 1104 82-83 3.9014232873101236 1104 84-85 3.975384788414665 1104 86-87 4.290713371870808 1104 88-89 4.16050949821058 1104 90-91 3.9306066751436806 1104 92-93 3.9103386865495704 1104 94-95 4.034046238246246 1104 96-97 3.730409306540837 1104 98-99 3.254334827545522 1104 100-101 2.1623152659648115 1105 1 0.5138703409144476 1105 2 0.4324410053521497 1105 3 0.3743251586656626 1105 4 0.42637877218373177 1105 5 0.4175097009908981 1105 6 0.4605067856467713 1105 7 0.48456059511800476 1105 8 0.4917418697641054 1105 9 0.5580706711365551 1105 10-11 0.5976971590881988 1105 12-13 0.5722303433156313 1105 14-15 0.6752271647033297 1105 16-17 0.7352286151966325 1105 18-19 0.6327327569135619 1105 20-21 0.6719475451323333 1105 22-23 0.7101726248354367 1105 24-25 0.7213875476915774 1105 26-27 0.6420479209313399 1105 28-29 0.7051687275090543 1105 30-31 0.8659054133398598 1105 32-33 0.800659928278165 1105 34-35 0.8410246239577717 1105 36-37 0.8964049273163326 1105 38-39 0.955274358078448 1105 40-41 1.0143132072356664 1105 42-43 1.0866826045915232 1105 44-45 1.1378978648024187 1105 46-47 1.3134556813917655 1105 48-49 1.2953039375810604 1105 50-51 1.2208981938066081 1105 52-53 1.4143168744727426 1105 54-55 1.6006154030996207 1105 56-57 1.679205614110149 1105 58-59 1.7067309722940252 1105 60-61 1.8223479449549522 1105 62-63 1.956645087537563 1105 64-65 2.0270436104248724 1105 66-67 2.163310082907838 1105 68-69 2.4760883387151402 1105 70-71 2.6063085728788167 1105 72-73 2.552830312373267 1105 74-75 2.804686161211311 1105 76-77 2.900511897624966 1105 78-79 3.2434908904282764 1105 80-81 3.4453366244568002 1105 82-83 3.5866973375730105 1105 84-85 3.563140258571387 1105 86-87 3.7404764904645127 1105 88-89 3.6970126751184296 1105 90-91 3.5880681224859057 1105 92-93 3.621123446612417 1105 94-95 3.541320451021134 1105 96-97 3.3445049027826617 1105 98-99 3.2450640799644006 1105 100-101 2.2361591040371644 1106 1 0.34473540517915424 1106 2 0.38461807411091087 1106 3 0.1878043347049605 1106 4 0.32791281199349953 1106 5 0.23962581519042914 1106 6 -0.058881173950304344 1106 7 0.3357129670903021 1106 8 -0.003847661478801001 1106 9 0.4569487417937239 1106 10-11 0.3969732750810522 1106 12-13 0.39784385953197443 1106 14-15 0.5850318057582129 1106 16-17 0.23407847780526936 1106 18-19 0.5292991677395733 1106 20-21 0.6407105079999624 1106 22-23 0.45706722912863285 1106 24-25 0.47843199336488595 1106 26-27 0.6106501495439645 1106 28-29 0.7019070525661917 1106 30-31 0.3827302166790574 1106 32-33 0.5102500778494203 1106 34-35 0.4448217921780184 1106 36-37 0.6036900995848384 1106 38-39 0.7253960875818564 1106 40-41 0.8320500024534425 1106 42-43 0.5366969030809088 1106 44-45 0.8957069826563426 1106 46-47 1.0626689462601817 1106 48-49 0.9746717689782542 1106 50-51 0.8822061562548988 1106 52-53 1.0121304572456502 1106 54-55 0.9327988701141656 1106 56-57 1.3653318506784409 1106 58-59 1.4630954265039975 1106 60-61 1.6386157153537404 1106 62-63 1.7620507792414877 1106 64-65 1.7935482534739045 1106 66-67 1.992912361444958 1106 68-69 2.0800279261125034 1106 70-71 1.8640405411189178 1106 72-73 2.1480439319460487 1106 74-75 2.5100842793830225 1106 76-77 2.7476720422617547 1106 78-79 3.1341878137921952 1106 80-81 2.8129190355894274 1106 82-83 2.8830284385411353 1106 84-85 2.798365824666398 1106 86-87 2.5017981786140346 1106 88-89 2.5123433358175404 1106 90-91 2.6700352735804866 1106 92-93 2.9166393851852046 1106 94-95 2.707174053158523 1106 96-97 2.6974058696914884 1106 98-99 2.426345094498087 1106 100-101 1.8591821893796299 1107 1 0.21041770653445724 1107 2 0.17166427006917573 1107 3 0.17863987711541185 1107 4 0.08180248280820024 1107 5 0.11380506648826128 1107 6 0.19021892786408756 1107 7 0.18338923941482932 1107 8 0.2773672407148524 1107 9 0.3567930674729354 1107 10-11 0.2811016437869611 1107 12-13 0.25352540493452835 1107 14-15 0.4137730678739615 1107 16-17 0.49358068770258967 1107 18-19 0.4411855610274742 1107 20-21 0.4570120412853953 1107 22-23 0.5115291821496584 1107 24-25 0.5136237811753759 1107 26-27 0.5101304422794897 1107 28-29 0.5476029474009962 1107 30-31 0.629435533993302 1107 32-33 0.7107373597987419 1107 34-35 0.6251828915329014 1107 36-37 0.5550640539641165 1107 38-39 0.9119795461326845 1107 40-41 0.9152217901873811 1107 42-43 0.9605249234197331 1107 44-45 0.7710070398517459 1107 46-47 0.7832415374092179 1107 48-49 0.7154402257082211 1107 50-51 0.9144548887695585 1107 52-53 0.9403933434377159 1107 54-55 0.9927620198097635 1107 56-57 1.049503642239987 1107 58-59 1.2475451366446464 1107 60-61 1.332508984120782 1107 62-63 1.5211466474470683 1107 64-65 1.6670336481386556 1107 66-67 1.788342934634855 1107 68-69 1.8578376575893323 1107 70-71 2.1072037139988886 1107 72-73 2.1644584275382854 1107 74-75 2.214598991891318 1107 76-77 2.2204589734927467 1107 78-79 2.5788832741933945 1107 80-81 2.73353825521653 1107 82-83 2.9327473006695683 1107 84-85 2.845804593860489 1107 86-87 2.7684320782147616 1107 88-89 2.9805314975154253 1107 90-91 2.8338248428541934 1107 92-93 2.851753178629929 1107 94-95 2.7664451233251537 1107 96-97 2.5976234214106864 1107 98-99 2.264912819662646 1107 100-101 1.4904884825700941 1108 1 0.1835867384655252 1108 2 0.2532192228368899 1108 3 0.12363900630598579 1108 4 0.0955550144128452 1108 5 0.1132072323529485 1108 6 0.2028334106241516 1108 7 0.2154596644324016 1108 8 0.18358979978910384 1108 9 0.2799610825906029 1108 10-11 0.2647824405097907 1108 12-13 0.20602803039886908 1108 14-15 0.27425577526739175 1108 16-17 0.34132806797411774 1108 18-19 0.31380361045608396 1108 20-21 0.3664759537956357 1108 22-23 0.3613437571007978 1108 24-25 0.46850112219268425 1108 26-27 0.4424703749925172 1108 28-29 0.4959466105155954 1108 30-31 0.4030116562945736 1108 32-33 0.5090884013285262 1108 34-35 0.7277800154631606 1108 36-37 0.690735966866491 1108 38-39 0.6986345611606595 1108 40-41 0.8399588917790268 1108 42-43 0.8523144028946987 1108 44-45 0.7474018471160733 1108 46-47 0.8121342901890287 1108 48-49 0.9885982160886471 1108 50-51 1.0190913101912606 1108 52-53 1.0668071051467116 1108 54-55 1.1076960234056337 1108 56-57 1.2880190607812416 1108 58-59 1.37502772567057 1108 60-61 1.4185079328921582 1108 62-63 1.5450852376535416 1108 64-65 1.7612357390198738 1108 66-67 1.8504694512511506 1108 68-69 2.0810015402730535 1108 70-71 2.1886417984058717 1108 72-73 2.342810235588942 1108 74-75 2.358546592628848 1108 76-77 2.5752134365214125 1108 78-79 2.8305509133508266 1108 80-81 2.90103714454343 1108 82-83 3.298666896280274 1108 84-85 3.41569552367638 1108 86-87 3.189182032849242 1108 88-89 3.1930230110343487 1108 90-91 3.2080969739282192 1108 92-93 3.1459829320500763 1108 94-95 3.027009032393879 1108 96-97 2.6685125882285714 1108 98-99 2.4779412236931915 1108 100-101 1.8066833608549233 1109 1 0.6550066207550316 1109 2 0.46700725641703755 1109 3 0.5206111250645442 1109 4 0.4794038300786525 1109 5 0.39630072433222807 1109 6 0.48146809080429875 1109 7 0.4610377933390737 1109 8 0.5270766153153801 1109 9 0.6106174485177931 1109 10-11 0.6169715907897881 1109 12-13 0.6135451498702409 1109 14-15 0.7587768251143103 1109 16-17 0.7601168331463413 1109 18-19 0.7319307289495853 1109 20-21 0.6203201704699595 1109 22-23 0.7235647825516764 1109 24-25 0.4497828729640432 1109 26-27 0.5718457903497622 1109 28-29 0.8374085120454637 1109 30-31 0.6867591034859757 1109 32-33 0.789674150203659 1109 34-35 0.8167101606246092 1109 36-37 0.9188038040130806 1109 38-39 1.0252630179693583 1109 40-41 0.8887933132081329 1109 42-43 1.1885322769386661 1109 44-45 1.2595113210240925 1109 46-47 1.3959242246086276 1109 48-49 1.4800673324308562 1109 50-51 1.3902077514847129 1109 52-53 1.538822119178402 1109 54-55 1.811952524355977 1109 56-57 1.7912395825674459 1109 58-59 1.8227947593080671 1109 60-61 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2314 16-17 -0.5376512177092465 2314 18-19 -0.47105982226759835 2314 20-21 -0.4876747413934339 2314 22-23 -0.5312195408894098 2314 24-25 -0.5132691712410278 2314 26-27 -0.5170605538534332 2314 28-29 -0.44793466866286025 2314 30-31 -0.6500139252370047 2314 32-33 -0.6072708913334637 2314 34-35 -0.6443672770052586 2314 36-37 -0.5805482927488725 2314 38-39 -0.5767401583568414 2314 40-41 -0.5868742093576671 2314 42-43 -0.5466267040592285 2314 44-45 -0.6551861689908165 2314 46-47 -0.6135658017557404 2314 48-49 -0.5075666554051566 2314 50-51 -0.534382533940704 2314 52-53 -0.39542696179213976 2314 54-55 -0.5091625981359336 2314 56-57 -0.3808822119279718 2314 58-59 -0.40777777420590056 2314 60-61 -0.3395783041725373 2314 62-63 -0.3439006828256943 2314 64-65 -0.39239117660233447 2314 66-67 -0.5890703664384098 2314 68-69 -0.7144304473385965 2314 70-71 -0.8536322301250223 2314 72-73 -0.6571131649922712 2314 74-75 -0.7768860826583825 2314 76-77 -0.5556577210912828 2314 78-79 -0.7337081034768502 2314 80-81 -0.803095530998494 2314 82-83 -0.8046919532130126 2314 84-85 -0.8606213113859056 2314 86-87 -0.7466099529286083 2314 88-89 -0.6986999219274708 2314 90-91 -0.8389062250367001 2314 92-93 -0.8505912337612411 2314 94-95 -0.8356254145494031 2314 96-97 -0.8367248890717676 2314 98-99 -0.915809206104047 2314 100-101 -0.6328226461186164 2315 1 -0.710499695735404 2315 2 -0.5018461010277164 2315 3 -0.4521927296962467 2315 4 -0.4495946702647231 2315 5 -0.3610553375615808 2315 6 -0.2840872439375275 2315 7 -0.4130696809739618 2315 8 -0.3721675908099584 2315 9 -1.2232164292032337 2315 10-11 -0.7659630140472871 2315 12-13 -0.6745682476012931 2315 14-15 -0.49772935948683994 2315 16-17 -0.5495325030768186 2315 18-19 -0.4948669094056868 2315 20-21 -0.46088014419559187 2315 22-23 -0.4693819798179746 2315 24-25 -0.5361681871275223 2315 26-27 -0.4555062501786935 2315 28-29 -0.5752929290695619 2315 30-31 -0.6836013136291541 2315 32-33 -0.6250974136043368 2315 34-35 -0.49721648521529005 2315 36-37 -0.5596647134399717 2315 38-39 -0.5204688902888819 2315 40-41 -0.4568486084654424 2315 42-43 -0.514145255200809 2315 44-45 -0.7260066965973238 2315 46-47 -0.5275062479970316 2315 48-49 -0.3758961655029367 2315 50-51 -0.4237481831197627 2315 52-53 -0.35062587565527537 2315 54-55 -0.3361905500011879 2315 56-57 -0.1764888706946941 2315 58-59 -0.09247284476677464 2315 60-61 -0.2907197300915385 2315 62-63 -0.20751557316642888 2315 64-65 -0.24872470426587157 2315 66-67 -0.13967965499975676 2315 68-69 -0.17325309145450873 2315 70-71 -0.22763861133751817 2315 72-73 -0.3234475164468975 2315 74-75 -0.477633356226729 2315 76-77 -0.5581629965226647 2315 78-79 -0.5192656199368315 2315 80-81 -0.39814327919724235 2315 82-83 -0.5207455820127471 2315 84-85 -0.715556453679973 2315 86-87 -0.5064754848758604 2315 88-89 -0.5385999263632684 2315 90-91 -0.4627982260252512 2315 92-93 -0.6560528102439083 2315 94-95 -0.6791249107694686 2315 96-97 -0.5383938581289076 2315 98-99 -0.708973007456331 2315 100-101 -0.29533017113335003 2316 1 -1.0287618800288492 2316 2 -0.8050918788445962 2316 3 -0.7857430781913166 2316 4 -0.832332841146247 2316 5 -0.6824505560593508 2316 6 -0.6740240618956932 2316 7 -0.884675886434529 2316 8 -0.8134871472648584 2316 9 -1.0928049801871325 2316 10-11 -1.0046948439152175 2316 12-13 -0.9277556985203503 2316 14-15 -1.0630351195123922 2316 16-17 -1.1407007028610963 2316 18-19 -1.183479478689243 2316 20-21 -1.216503965031002 2316 22-23 -1.2839249282915404 2316 24-25 -1.0537068246242214 2316 26-27 -1.3464419569931678 2316 28-29 -1.2114541394015674 2316 30-31 -1.183246301589051 2316 32-33 -1.3017159850189444 2316 34-35 -1.037996599327471 2316 36-37 -1.2874669298620844 2316 38-39 -1.3703626636481516 2316 40-41 -1.3011607583818616 2316 42-43 -1.0189904608275668 2316 44-45 -1.1489135267096415 2316 46-47 -0.8201140468572206 2316 48-49 -0.6239648609029302 2316 50-51 -0.5469463218726105 2316 52-53 -0.6366846066053533 2316 54-55 -0.8251099057333526 2316 56-57 -0.679268847950464 2316 58-59 -0.38309655155517675 2316 60-61 -0.49690531389816783 2316 62-63 -0.47384270560106856 2316 64-65 -0.4436808939227852 2316 66-67 -0.39933291951386707 2316 68-69 -0.31603244959795873 2316 70-71 -0.5761625172194087 2316 72-73 -0.4843608374631536 2316 74-75 -0.44085547743588904 2316 76-77 -0.2644893469206906 2316 78-79 -0.35211311302069603 2316 80-81 -0.36808390958393034 2316 82-83 -0.401622016968485 2316 84-85 -0.22289456395365193 2316 86-87 -0.11659878598277018 2316 88-89 -0.309938284281877 2316 90-91 -0.3889578396991613 2316 92-93 -0.20704371580972847 2316 94-95 -0.137258580378548 2316 96-97 0.015033580277561853 2316 98-99 -0.26341285743564136 2316 100-101 0.1985269312254685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12837.0 3 5074.0 4 5634.0 5 6608.0 6 8025.0 7 10378.0 8 13340.0 9 17161.0 10 21346.0 11 26404.0 12 32444.0 13 38313.0 14 43646.0 15 49704.0 16 57020.0 17 64590.0 18 65403.0 19 67090.0 20 72217.0 21 79813.0 22 88533.0 23 98045.0 24 107021.0 25 118432.0 26 130028.0 27 141779.0 28 155897.0 29 170247.0 30 187055.0 31 207211.0 32 226849.0 33 252615.0 34 278467.0 35 294317.0 36 278866.0 37 185567.0 38 42074.0 39 530.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.30777656679635 18.416367227588143 14.740054335097149 23.535801870518355 2 15.62677961470146 19.617078835906547 40.2521382702555 24.504003279136494 3 19.500046867360997 23.540013385208987 28.950447125555062 28.009492621874955 4 13.050653042361049 16.258102390548547 36.46323768752301 34.228006879567396 5 14.247928643086958 37.005765083113964 33.78713202833064 14.959174245468438 6 33.130996510673796 35.25066788097915 17.3064949136087 14.31184069473835 7 29.651206167110256 31.81536820132965 19.99623956691617 18.537186064643922 8 26.710391754853823 34.73026235599498 19.415349347949782 19.143996541201407 9 26.57583243081193 16.797774809910273 19.699480185931705 36.92691257334609 10-11 25.278067349422628 26.28046121760014 27.068528797951323 21.3729426350259 12-13 26.25177247501046 23.420772245739844 27.31799014655557 23.009465132694125 14-15 22.97389559522412 25.090363713348474 25.573282700050676 26.36245799137673 16-17 22.75561885137667 28.11940230807811 26.307825084026433 22.817153756518785 18-19 22.69677907515792 26.7936438711324 28.38182899038667 22.127748063323008 20-21 23.330115943541916 25.981347155356477 28.55761828621524 22.130918614886365 22-23 22.969651208611868 25.920744334606248 28.41589234289849 22.69371211388339 24-25 22.735882122650438 26.050191886845177 28.56756145537478 22.646364535129607 26-27 22.369194691828998 26.524130167946698 28.302306512045643 22.804368628178658 28-29 22.482411982028104 26.77802977853765 28.227291477341588 22.512266762092654 30-31 22.66600278578555 26.33262836981262 28.22710738227706 22.774261462124766 32-33 22.35868533054605 26.27812732700729 28.271118124867638 23.092069217579024 34-35 23.022062622141814 26.448421056371025 28.019720040678525 22.509796280808636 36-37 22.98625957035504 26.107786443865532 27.94998670527829 22.955967280501138 38-39 22.52767714699223 26.44974588582581 28.01668538722596 23.005891579956007 40-41 23.08556838626742 26.169019318015714 27.994975485341065 22.750436810375803 42-43 22.748854789556137 26.21697222915903 27.915525736702417 23.118647244582416 44-45 22.732376222542943 26.471023367227687 27.97486986521718 22.82173054501219 46-47 22.99898424035592 26.65759063451848 27.32083318248358 23.022591942642027 48-49 22.88912201426886 26.630779808949818 27.404729354685482 23.07536882209584 50-51 23.104120476430516 26.792590983665903 27.281917197043278 22.821371342860306 52-53 23.126180430581734 26.60964081032487 27.335186414116563 22.928992344976834 54-55 22.778838874516804 26.67798918054706 27.496373890481518 23.046798054454616 56-57 22.893238914949006 26.86679688604566 27.250993686604968 22.98897051240037 58-59 22.91613422468958 26.513827873865104 27.267881820487816 23.3021560809575 60-61 22.699601629326903 26.6685554087394 27.53764085824476 23.094202103688936 62-63 22.696253683088482 26.638013858207394 27.68100632175013 22.984726136953995 64-65 22.570805516912785 26.54272536628065 27.534663749607958 23.351805367198608 66-67 22.564472213468704 26.622898626624412 27.3818934287428 23.43073573116408 68-69 22.849092444472856 26.389950952469547 27.52591503436715 23.23504156869045 70-71 22.713521652537285 26.3790918389107 27.29559708905179 23.61178941950023 72-73 22.775115002651543 26.418575499852054 27.275593537174743 23.53071596032166 74-75 22.752846978054478 26.700475313713333 27.038743928117988 23.5079337801142 76-77 22.841922967853822 26.378791927093154 27.21394612853116 23.565338976521865 78-79 22.896420114241064 26.41178828998352 27.18750192097928 23.504289674796137 80-81 22.94828014396088 26.55068998330948 26.92362439794315 23.57740547478649 82-83 22.95713593302008 26.525102443060018 26.970406673948833 23.547354949971066 84-85 23.027598229748563 26.35864940054985 26.981075991367852 23.632676378333738 86-87 23.02681184284431 26.370806824912197 26.83973542209857 23.762645910144922 88-89 23.015758736896636 26.375405812885354 26.736231597365855 23.872603852852155 90-91 23.04938754352585 26.284639306898256 26.748773843748797 23.9171993058271 92-93 23.033137571802033 25.969710808339713 26.89997310219894 24.097178517659316 94-95 22.884371306979254 25.969069136763874 26.886611881877986 24.25994767437889 96-97 23.145888768832247 25.702963225370247 26.74026082787898 24.41088717791853 98-99 22.91222349904948 25.748801813276856 26.471497882306398 24.867476805367264 100-101 23.97153937173926 24.728569326350836 26.685846108230916 24.61404519367899 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 405.0 1 296.0 2 236.5 3 308.0 4 294.5 5 292.0 6 453.5 7 1011.5 8 1723.5 9 2011.5 10 1829.0 11 1624.5 12 1697.5 13 1921.5 14 2169.0 15 2473.5 16 2747.5 17 2973.0 18 3332.0 19 3784.5 20 4360.5 21 5171.0 22 6267.5 23 7714.5 24 9495.5 25 11929.0 26 15312.5 27 19524.0 28 23809.5 29 28815.5 30 34810.5 31 42334.5 32 51844.0 33 58509.5 34 63601.0 35 70416.0 36 77948.5 37 85552.0 38 92044.0 39 97559.5 40 102492.0 41 109477.0 42 116798.0 43 122447.5 44 128045.5 45 133715.5 46 138049.5 47 141268.0 48 142407.0 49 141211.5 50 139078.0 51 135791.0 52 133500.5 53 132887.5 54 131650.0 55 124542.0 56 113810.0 57 104044.5 58 95121.5 59 84584.0 60 71080.0 61 59179.0 62 49549.5 63 39413.0 64 30685.5 65 24284.0 66 18842.5 67 14567.0 68 10934.5 69 8099.0 70 6315.5 71 4778.0 72 3487.0 73 2771.0 74 2200.0 75 1672.5 76 1201.5 77 784.5 78 505.0 79 327.5 80 205.5 81 125.5 82 87.0 83 50.5 84 29.5 85 22.5 86 17.5 87 13.0 88 9.0 89 7.0 90 5.0 91 4.0 92 3.0 93 1.5 94 1.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3911402018259401 2 0.029503521299903294 3 0.0359779051407154 4 0.04450114462735413 5 0.06419747690256736 6 0.054472242103710336 7 0.038982893421261114 8 0.041141021368198485 9 0.03789017041015358 10-11 0.039324369362232214 12-13 0.03138846849406378 14-15 0.029052773057821436 16-17 0.02372574837867223 18-19 0.028014686197269288 20-21 0.03548617978571702 22-23 0.034366138699331805 24-25 0.04159176961028034 26-27 0.036975014888351025 28-29 0.03276803129558704 30-31 0.03521299903294013 32-33 0.030186473181845502 34-35 0.03156603598336876 36-37 0.0336831868173896 38-39 0.016691343994667512 40-41 0.014341989520786323 42-43 0.01513421370383928 44-45 0.011815067557600163 46-47 0.020693442022848837 48-49 0.017797726043413887 50-51 0.012156543498571265 52-53 0.01008036977746696 54-55 0.008769102164137925 56-57 0.009465713083718973 58-59 0.004521141458457403 60-61 0.008072491244556874 62-63 0.011978976009266291 64-65 0.0074305164755312 66-67 0.004002098028181326 68-69 0.006911473045255123 70-71 0.01462882931120205 72-73 0.01221117964912664 74-75 0.012866813455791158 76-77 0.0065017019160898 78-79 0.00921985040621978 80-81 0.01144627354135137 82-83 0.013604401488288741 84-85 0.008441285260805665 86-87 0.0029503521299903295 88-89 0.01753820432827585 90-91 0.026580487245190652 92-93 0.011733113331767096 94-95 0.02199105059853903 96-97 0.02003780821618432 98-99 0.012730223079402718 100-101 0.1251714209223675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3660580.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.44106507793808 #Duplication Level Percentage of deduplicated Percentage of total 1 76.69623892783957 41.75424934703603 2 12.972067986124594 14.124263948560925 3 4.3466668568692075 7.099115196807991 4 1.9472103549310795 4.240328226129512 5 1.1091466005790407 3.0191561131548674 6 0.6283456585182151 2.0524684132098003 7 0.4133526900762084 1.5752352490405745 8 0.2973311289792404 1.2949618673964503 9 0.22933029565621477 1.123648699514649 >10 1.2110484756064077 12.272713972057982 >50 0.09602550167936298 3.5583799950398634 >100 0.048792205312924386 4.786588437939525 >500 0.002575104661598896 0.9677057760612338 >1k 0.0017672286709330695 1.68938930556265 >5k 1.0098449548188967E-4 0.4417954524879252 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9072 0.24782957891918764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7000 0.19122652694381764 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4750 0.12976085756901912 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.7318075277688236E-5 0.0 0.0 0.0 0.0 8 2.7318075277688236E-5 0.0 0.0 0.0 0.0 9 2.7318075277688236E-5 0.0 0.0 0.0 0.0 10-11 2.7318075277688236E-5 0.0 0.0 1.3659037638844118E-5 0.0 12-13 2.7318075277688236E-5 0.0 0.0 2.7318075277688236E-5 0.0 14-15 2.7318075277688236E-5 0.0 0.0 2.7318075277688236E-5 0.0 16-17 2.7318075277688236E-5 0.0 0.0 2.7318075277688236E-5 0.0 18-19 2.7318075277688236E-5 0.0 0.0 5.463615055537647E-5 0.0 20-21 2.7318075277688236E-5 0.0 0.0 1.2293133874959706E-4 0.0 22-23 2.7318075277688236E-5 0.0 0.0 2.8683979041572646E-4 0.0 24-25 2.7318075277688236E-5 0.0 0.0 6.146566937479854E-4 0.0 26-27 2.7318075277688236E-5 0.0 0.0 0.0011200410863852176 0.0 28-29 2.7318075277688236E-5 0.0 0.0 0.002882056941796109 0.0 30-31 2.7318075277688236E-5 0.0 0.0 0.009465713083718973 0.0 32-33 2.7318075277688236E-5 0.0 0.0 0.02462724486283594 0.0 34-35 2.7318075277688236E-5 0.0 0.0 0.04787492692414863 0.0 36-37 2.7318075277688236E-5 0.0 0.0 0.08564216599555262 0.0 38-39 2.7318075277688236E-5 0.0 0.0 0.143966256713417 0.0 40-41 2.7318075277688236E-5 0.0 0.0 0.20843691436876122 0.0 42-43 2.7318075277688236E-5 0.0 0.0 0.2753935168743751 0.0 44-45 2.7318075277688236E-5 0.0 0.0 0.3596424610307656 0.0 46-47 2.7318075277688236E-5 0.0 0.0 0.46168913123057 0.0 48-49 2.7318075277688236E-5 0.0 0.0 0.5726688120461785 0.0 50-51 2.7318075277688236E-5 0.0 0.0 0.6939474072414754 0.0 52-53 2.7318075277688236E-5 0.0 0.0 0.8116473345753952 0.0 54-55 2.7318075277688236E-5 0.0 0.0 0.9451780865327353 0.0 56-57 2.7318075277688236E-5 0.0 0.0 1.100645252938059 0.0 58-59 2.7318075277688236E-5 0.0 0.0 1.2668484229275143 0.0 60-61 2.7318075277688236E-5 0.0 0.0 1.4516552021810751 0.0 62-63 2.7318075277688236E-5 0.0 0.0 1.6373088417682444 0.0 64-65 2.7318075277688236E-5 0.0 0.0 1.8306525195460828 0.0 66-67 2.7318075277688236E-5 0.0 0.0 2.053349469209797 0.0 68-69 2.7318075277688236E-5 0.0 0.0 2.299089215370242 5.463615055537647E-5 70-71 2.7318075277688236E-5 0.0 0.0 2.562408142971879 8.19542258330647E-5 72-73 2.7318075277688236E-5 0.0 0.0 2.834810330603347 8.19542258330647E-5 74-75 2.7318075277688236E-5 0.0 0.0 3.110436051117582 8.19542258330647E-5 76-77 2.7318075277688236E-5 0.0 0.0 3.411836375656317 8.19542258330647E-5 78-79 2.7318075277688236E-5 0.0 0.0 3.7418387250107905 8.19542258330647E-5 80-81 2.7318075277688236E-5 0.0 0.0 4.092452562162281 8.19542258330647E-5 82-83 2.7318075277688236E-5 0.0 0.0 4.454266810177622 8.19542258330647E-5 84-85 2.7318075277688236E-5 0.0 0.0 4.8163815570210184 8.19542258330647E-5 86-87 4.097711291653235E-5 0.0 0.0 5.18883619535702 8.19542258330647E-5 88-89 5.463615055537647E-5 0.0 0.0 5.580713985215457 8.19542258330647E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5475 0.0 54.65597 1 GTATCAA 10255 0.0 45.747993 1 ATCAACG 13430 0.0 34.64874 3 TATCAAC 13635 0.0 34.267242 2 TCAACGC 13675 0.0 34.062737 4 CAACGCA 14025 0.0 33.14445 5 AACGCAG 14655 0.0 31.711374 6 ACGCAGA 16830 0.0 27.520971 7 CGCAGAG 16870 0.0 27.31492 8 GTACATG 13600 0.0 25.44212 1 TACATGG 13405 0.0 24.997873 2 GCAGAGT 18735 0.0 23.959942 9 ACATGGG 14160 0.0 22.792255 3 TGGTATC 2475 0.0 22.661444 2 GAGTACT 10270 0.0 22.177723 12-13 GTGGTAT 2960 0.0 20.960701 1 AGTACTT 10775 0.0 20.917881 12-13 GTAAGGT 1595 0.0 20.860186 4 AGAGTAC 17040 0.0 20.808947 10-11 CAGAGTA 17805 0.0 20.408417 10-11 >>END_MODULE