FastQCFastQC Report
Thu 26 May 2016
SRR938572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938572_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3660580
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95050.25965830551442665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78180.21357271252096666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52200.1426003529495326No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60300.054.452321
GTATCAA103700.040.8276671
ATCAACG135500.030.953163
TCAACGC136150.030.840274
TATCAAC136400.030.7522812
CAACGCA140350.029.9173685
AACGCAG146800.028.764666
ACGCAGA169800.024.7285217
CGCAGAG171150.024.4502098
TGGTATC27700.024.0092892
GTACATG135500.023.495851
TACATGG133450.023.2803882
GTGGTAT33550.021.5281491
GCAGAGT191400.021.3174279
ACATGGG141000.021.1555393
GAGTACT105550.021.15004312-13
TAAGGTG21400.019.9761735
AGTACTT109450.019.68036812-13
GTACTTT112300.019.66730714-15
GTAAGGT20550.019.415614
Produced by FastQC (version 0.11.5)