FastQCFastQC Report
Thu 26 May 2016
SRR938571_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938571_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2603793
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99920.38374786321339677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76210.29268839727274787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54110.20781221855961668No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA38870.1492822202072131No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA38110.1463634013917389No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35200.1351873977693311No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33210.12754470113407632No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32310.12408820516838319No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA30410.11679115812969772No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA29830.11456363850736215No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28370.10895643394079328No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51150.058.5787351
GTATCAA99650.046.4864851
TATCAAC134650.034.156512
ATCAACG137400.033.50753
TCAACGC137800.033.3412064
CAACGCA140750.032.6761975
AACGCAG146800.031.4313056
TAAGGTG18450.028.3563125
TGGTATC21150.028.334372
ACGCAGA165500.027.82517
CGCAGAG167850.027.3789068
GTGGTAT25200.025.8562221
GCAGAGT180250.024.5457929
AGGTAAG15900.024.2293242
GTAAGGT19650.023.2360384
TACATGG128200.023.0015472
GTGTAAG11700.022.763931
GGTAAGG18400.022.746693
GTACATG132900.022.7244721
AGTACTT101650.022.25241712-13
Produced by FastQC (version 0.11.5)