##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938571_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2603793 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.176003238352664 31.0 31.0 34.0 28.0 34.0 2 31.207080209525106 31.0 31.0 34.0 28.0 34.0 3 31.238279310221664 31.0 31.0 34.0 27.0 34.0 4 34.91211282924564 37.0 35.0 37.0 32.0 37.0 5 34.896729117867665 37.0 35.0 37.0 32.0 37.0 6 34.92531818005502 37.0 35.0 37.0 32.0 37.0 7 34.83776782562976 37.0 35.0 37.0 32.0 37.0 8 34.80344405258022 37.0 35.0 37.0 32.0 37.0 9 36.30530921620881 39.0 35.0 39.0 32.0 39.0 10-11 36.14772506877467 39.0 35.0 39.0 31.0 39.0 12-13 36.01494588855566 38.0 35.0 39.0 30.0 39.0 14-15 36.968514202165835 39.0 36.0 41.0 31.0 41.0 16-17 36.810317102780445 39.0 36.0 41.0 31.0 41.0 18-19 36.979731683739836 39.0 36.0 41.0 31.0 41.0 20-21 36.96693439148196 39.0 36.0 41.0 31.0 41.0 22-23 36.884963397628 39.0 36.0 41.0 30.0 41.0 24-25 36.745605353420956 39.0 36.0 41.0 30.0 41.0 26-27 36.53795770247481 39.0 36.0 41.0 30.0 41.0 28-29 36.34057123588549 39.0 35.0 40.5 29.5 41.0 30-31 36.06968603110923 38.5 35.0 40.0 29.0 41.0 32-33 35.78326982982134 38.0 35.0 40.0 27.5 41.0 34-35 35.53148598986171 38.0 34.5 40.0 27.0 41.0 36-37 35.24247492024136 38.0 34.0 40.0 26.0 41.0 38-39 35.093976748535695 38.0 34.0 40.0 25.0 41.0 40-41 34.986462441522804 38.0 34.0 40.0 25.0 41.0 42-43 34.67428113525153 38.0 33.5 40.0 24.0 41.0 44-45 34.30713751054711 38.0 33.0 40.0 23.5 41.0 46-47 33.87605274305599 38.0 33.0 40.0 22.5 41.0 48-49 33.48274536416682 38.0 32.5 40.0 19.5 41.0 50-51 32.758935714167755 36.5 31.5 39.5 18.0 40.5 52-53 32.765061009074074 37.0 31.5 39.0 18.0 40.0 54-55 32.87243609611056 37.0 32.0 40.0 17.0 41.0 56-57 32.56233617649329 36.5 31.0 40.0 13.5 41.0 58-59 32.19980870215105 36.0 31.0 39.0 10.5 41.0 60-61 31.73823130333325 35.5 30.5 39.0 8.0 40.5 62-63 31.1886407636859 35.0 29.5 39.0 6.5 40.0 64-65 30.689918130972778 35.0 29.0 38.0 2.0 40.0 66-67 30.178078288097403 34.5 28.5 38.0 2.0 40.0 68-69 29.611243290077205 34.0 28.0 37.0 2.0 39.5 70-71 28.995780194508548 34.0 26.5 36.5 2.0 39.0 72-73 28.396725085289038 34.0 26.0 36.0 2.0 39.0 74-75 27.761342011442537 33.0 26.0 35.5 2.0 38.5 76-77 27.05929119557507 32.5 24.5 35.0 2.0 37.5 78-79 26.317140609871828 32.0 22.5 35.0 2.0 37.0 80-81 25.661502469666367 31.5 20.5 35.0 2.0 36.0 82-83 24.96031155318414 31.0 18.5 34.5 2.0 36.0 84-85 24.288627398568167 31.0 14.5 34.0 2.0 35.0 86-87 23.56382669436472 30.5 7.0 34.0 2.0 35.0 88-89 22.762327304820314 29.5 2.0 34.0 2.0 35.0 90-91 21.875391400161227 29.0 2.0 34.0 2.0 35.0 92-93 20.95555695095578 29.0 2.0 34.0 2.0 35.0 94-95 19.953543542055762 27.0 2.0 33.5 2.0 35.0 96-97 18.718046903114036 25.0 2.0 33.0 2.0 34.5 98-99 17.202362092531935 21.0 2.0 32.0 2.0 34.0 100-101 13.618128630040868 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4180410975887625 1101 2 0.3216932731229498 1101 3 0.370150494391698 1101 4 0.2448241652065022 1101 5 0.32173095101843074 1101 6 0.5033500511631885 1101 7 0.33948696028259207 1101 8 0.37579346133133384 1101 9 0.6488660677011353 1101 10-11 0.6282295972033936 1101 12-13 0.7117164582992288 1101 14-15 0.8383902861081012 1101 16-17 0.6882627631749259 1101 18-19 0.8584154492195282 1101 20-21 0.8227591947393975 1101 22-23 0.8291230470187756 1101 24-25 0.9413871248539252 1101 26-27 0.8306074402134414 1101 28-29 0.8286835995457338 1101 30-31 0.8447746306596997 1101 32-33 0.8057938093639336 1101 34-35 0.7625296839471432 1101 36-37 0.8534659708521772 1101 38-39 0.9950512644366043 1101 40-41 0.9707221640516579 1101 42-43 1.114366836767438 1101 44-45 1.1020294328183695 1101 46-47 1.1570994734145614 1101 48-49 0.9180247609365821 1101 50-51 1.0262157550273514 1101 52-53 0.8574492850820761 1101 54-55 0.8339079926938737 1101 56-57 0.8192434886891959 1101 58-59 0.8890385249114914 1101 60-61 0.9732936918265374 1101 62-63 0.8512975201155903 1101 64-65 0.7935930429112865 1101 66-67 0.732679307066487 1101 68-69 0.6341339122493288 1101 70-71 0.8340074930306969 1101 72-73 0.9188470529416435 1101 74-75 0.7576124116268765 1101 76-77 0.7162409873493978 1101 78-79 0.9712122210110898 1101 80-81 1.0893041252794937 1101 82-83 0.8678496081621176 1101 84-85 1.036053290113614 1101 86-87 1.2194266613944968 1101 88-89 0.9299823633504012 1101 90-91 0.8486405413370051 1101 92-93 0.5321623330978085 1101 94-95 0.6414945355615593 1101 96-97 0.6724549025042101 1101 98-99 0.7633643367836065 1101 100-101 0.8460295775741216 1102 1 0.5016590022607232 1102 2 0.3752574691537305 1102 3 0.3771308901618049 1102 4 0.24484336057503953 1102 5 0.34938379893927873 1102 6 0.4551058515236761 1102 7 0.45515441203983187 1102 8 0.532617702778353 1102 9 0.6627961466516865 1102 10-11 0.6828773717883365 1102 12-13 0.7211423440215228 1102 14-15 0.7991508737935007 1102 16-17 0.8357645339476747 1102 18-19 0.7834776902020195 1102 20-21 0.8208089452955818 1102 22-23 0.796323200965638 1102 24-25 0.728712279040721 1102 26-27 0.6496047125515645 1102 28-29 0.42929780335064294 1102 30-31 0.6445170288138726 1102 32-33 0.7389569757551442 1102 34-35 0.6188002829770127 1102 36-37 0.7633998620029203 1102 38-39 0.7073354844999926 1102 40-41 0.6893302982810354 1102 42-43 0.8744624009097208 1102 44-45 0.9345109716726725 1102 46-47 0.9540191926247061 1102 48-49 0.9111744137883449 1102 50-51 0.9267132030031036 1102 52-53 0.7262777341538893 1102 54-55 0.6655888447378899 1102 56-57 0.7644467100559424 1102 58-59 0.616529315933434 1102 60-61 0.6538225338275403 1102 62-63 0.705819661013436 1102 64-65 0.8557284508800471 1102 66-67 0.6249949691435397 1102 68-69 0.6922154983242415 1102 70-71 0.8438278435765163 1102 72-73 0.877823431121115 1102 74-75 0.7845543110853761 1102 76-77 0.7673556144121783 1102 78-79 0.9575496775095864 1102 80-81 1.0941456771098679 1102 82-83 1.1476074681856545 1102 84-85 1.0456579327054492 1102 86-87 1.3247492491605222 1102 88-89 1.26745541785181 1102 90-91 1.3371122828752036 1102 92-93 1.1335846619183094 1102 94-95 1.2745671478016796 1102 96-97 1.3630306003998207 1102 98-99 1.3955563614918844 1102 100-101 1.2093376785955083 1103 1 0.4281770883235225 1103 2 0.41019111474311387 1103 3 0.442525224416368 1103 4 0.4264439318112281 1103 5 0.4526280801029188 1103 6 0.48008356073337666 1103 7 0.36994994722228114 1103 8 0.37613635489773856 1103 9 0.47843373724848703 1103 10-11 0.6612007343864263 1103 12-13 0.6433706091135889 1103 14-15 0.8075274938067523 1103 16-17 0.8264213630688815 1103 18-19 0.800118088883444 1103 20-21 0.6818614775663505 1103 22-23 0.7735019638545211 1103 24-25 0.8201133060819501 1103 26-27 0.7647778037754662 1103 28-29 0.8327338223079934 1103 30-31 0.8295685103652417 1103 32-33 0.7908750936355062 1103 34-35 0.7255680813868679 1103 36-37 0.8351493838146027 1103 38-39 0.9320724346759093 1103 40-41 1.062047573366037 1103 42-43 1.0132817659644147 1103 44-45 1.0140613451590283 1103 46-47 0.58537021800106 1103 48-49 0.681998266598832 1103 50-51 0.9726461049495185 1103 52-53 0.9111941760627857 1103 54-55 0.9394950236339739 1103 56-57 0.9596358765468764 1103 58-59 0.8277992990257275 1103 60-61 0.8498330373219183 1103 62-63 0.9868436314749012 1103 64-65 0.9824918281290138 1103 66-67 0.812744006292391 1103 68-69 1.1685645998481569 1103 70-71 1.140414670366905 1103 72-73 1.0606814434115748 1103 74-75 1.1847475287655804 1103 76-77 1.1815041617185997 1103 78-79 0.9493654427403939 1103 80-81 1.2066050264777708 1103 82-83 1.3449276712260811 1103 84-85 1.37444940189976 1103 86-87 1.3440127466502645 1103 88-89 1.3090841624271015 1103 90-91 1.2617129910008913 1103 92-93 1.1461049641111352 1103 94-95 1.3526787941384057 1103 96-97 1.5935576064784591 1103 98-99 1.561067329300652 1103 100-101 1.5694000743097565 1104 1 0.5437361384270964 1104 2 0.45238748561151 1104 3 0.5852024020035493 1104 4 0.5126155724274 1104 5 0.5019815541293582 1104 6 0.49154441324694886 1104 7 0.5934291591582195 1104 8 0.6551594607187994 1104 9 0.7320076192179528 1104 10-11 0.732521756553588 1104 12-13 0.8362535015320063 1104 14-15 0.8014049685886349 1104 16-17 0.8099618047042441 1104 18-19 0.8201329990208848 1104 20-21 0.8526886181079689 1104 22-23 0.8411735749706182 1104 24-25 0.9211747750432764 1104 26-27 0.9037751066682844 1104 28-29 0.9155924120028871 1104 30-31 0.9565032481985369 1104 32-33 0.9060152871737728 1104 34-35 0.7982464033989984 1104 36-37 0.9899101767647025 1104 38-39 1.123760129409753 1104 40-41 1.0215187719554848 1104 42-43 1.0527656866456923 1104 44-45 0.8711942701164688 1104 46-47 0.9369620211908014 1104 48-49 0.6692314058246538 1104 50-51 0.4869447459004306 1104 52-53 0.827389879251136 1104 54-55 0.7964156666584827 1104 56-57 0.7960714612842779 1104 58-59 0.8683794661414197 1104 60-61 0.9378657026490806 1104 62-63 1.0549375828332863 1104 64-65 0.97378826887849 1104 66-67 0.7798540190322711 1104 68-69 1.129315716181324 1104 70-71 1.0212034667431666 1104 72-73 1.0516518268206134 1104 74-75 0.889615678263052 1104 76-77 0.9456175007029017 1104 78-79 1.1881514124463415 1104 80-81 1.2397869755164699 1104 82-83 1.19084041386418 1104 84-85 1.2647519718453246 1104 86-87 1.65414108530992 1104 88-89 1.5540668522585896 1104 90-91 1.38675816762035 1104 92-93 1.3160163423337927 1104 94-95 1.223995250465105 1104 96-97 1.3406749749652036 1104 98-99 1.363994759417352 1104 100-101 1.232865538322189 1105 1 0.49555993382175956 1105 2 0.37350016245746787 1105 3 0.4144152485980186 1105 4 0.35878556626890656 1105 5 0.38501736739841164 1105 6 0.4666705865118317 1105 7 0.5105466127366185 1105 8 0.40370178874216833 1105 9 0.5580170088497312 1105 10-11 0.579953728934079 1105 12-13 0.44613984548119134 1105 14-15 0.6192312040566961 1105 16-17 0.6445725022749471 1105 18-19 0.5302984072217285 1105 20-21 0.6595601660736179 1105 22-23 0.7104306171593251 1105 24-25 0.7545412684206099 1105 26-27 0.6548095665579083 1105 28-29 0.5732073702177232 1105 30-31 0.5871847334706288 1105 32-33 0.6789240651573039 1105 34-35 0.7385090759102582 1105 36-37 0.8283228981940596 1105 38-39 0.7626161275629002 1105 40-41 0.7581758236144793 1105 42-43 0.5830583001475347 1105 44-45 0.6511243361457062 1105 46-47 0.5644415248411505 1105 48-49 0.6116735081770628 1105 50-51 0.6822354154649446 1105 52-53 0.7372502712506375 1105 54-55 0.6303359749232413 1105 56-57 0.7406862490621009 1105 58-59 0.845075402167268 1105 60-61 0.6850729173753969 1105 62-63 0.8827917106756509 1105 64-65 0.7780282301318202 1105 66-67 0.9649040661096997 1105 68-69 0.8643081617331632 1105 70-71 0.900626240302639 1105 72-73 0.9387367484287239 1105 74-75 1.1071520303125624 1105 76-77 1.2124081100594601 1105 78-79 1.0981646778690255 1105 80-81 1.3542599436874632 1105 82-83 1.4641986087999967 1105 84-85 1.3520668639789335 1105 86-87 1.3477283683768384 1105 88-89 1.4954136895983154 1105 90-91 1.3242798674266858 1105 92-93 1.528031195785367 1105 94-95 1.3447838019318077 1105 96-97 1.4121345887715293 1105 98-99 1.6034079587166623 1105 100-101 1.4116074490629362 1106 1 0.25514180379812856 1106 2 0.24168132670571651 1106 3 0.16113464103003494 1106 4 0.15784044946224185 1106 5 0.275721963669433 1106 6 0.11285668198455312 1106 7 0.3830263254370294 1106 8 0.37166320241512807 1106 9 0.19481219111445824 1106 10-11 0.2256472132970444 1106 12-13 0.283321128718967 1106 14-15 0.5386519392737696 1106 16-17 0.45774941067830355 1106 18-19 0.2160420247327295 1106 20-21 0.3221151987293993 1106 22-23 0.2832754936513808 1106 24-25 0.1927524675047323 1106 26-27 0.1241178698018146 1106 28-29 0.4850809770832356 1106 30-31 0.05526631136294924 1106 32-33 0.20688826216249367 1106 34-35 9.216804527838462E-4 1106 36-37 0.1280397556378361 1106 38-39 0.015111970875942404 1106 40-41 0.28792624550214185 1106 42-43 -0.12777884022873565 1106 44-45 0.18207874961372283 1106 46-47 0.23593417727246901 1106 48-49 0.6995986832512884 1106 50-51 0.43914769116243235 1106 52-53 0.42932977007393447 1106 54-55 0.4580294673017633 1106 56-57 -0.18290326554216563 1106 58-59 -0.09291301977871314 1106 60-61 0.4085036687189394 1106 62-63 0.46986018880237523 1106 64-65 0.5371047055708473 1106 66-67 0.2679023257669577 1106 68-69 0.10258067980996799 1106 70-71 0.006840423384264938 1106 72-73 0.4690315370329827 1106 74-75 0.9480181192788599 1106 76-77 0.7775292396003586 1106 78-79 1.057844019753297 1106 80-81 1.055638936936571 1106 82-83 0.8228967500619824 1106 84-85 0.9227621898464662 1106 86-87 0.6608368681676744 1106 88-89 0.7920745477518842 1106 90-91 0.8861088843147691 1106 92-93 0.7872226529731172 1106 94-95 0.5550141095453007 1106 96-97 0.8403805878005421 1106 98-99 1.1777835988381646 1106 100-101 0.670296206598076 1107 1 0.3362565017161181 1107 2 0.14480144309510123 1107 3 0.28913240647503713 1107 4 0.29569645829662505 1107 5 0.2329673068030118 1107 6 0.47871977141636535 1107 7 0.3944006808629581 1107 8 0.3182600689817221 1107 9 0.42794598550052143 1107 10-11 0.5758293454827026 1107 12-13 0.5530880106075671 1107 14-15 0.5896318486937204 1107 16-17 0.696182026095947 1107 18-19 0.651527563871177 1107 20-21 0.7720133336844626 1107 22-23 0.7619027619552199 1107 24-25 0.7825141686002866 1107 26-27 0.6842466379263072 1107 28-29 0.756005628315954 1107 30-31 0.7924484791057296 1107 32-33 0.8174077461760589 1107 34-35 0.7084315049642314 1107 36-37 0.829136929171824 1107 38-39 0.8481156911233967 1107 40-41 0.9714123730229289 1107 42-43 1.0200089597877877 1107 44-45 0.8572840581419783 1107 46-47 0.8441799664247398 1107 48-49 0.9112288741334922 1107 50-51 0.7671173759520116 1107 52-53 0.9215982611585005 1107 54-55 0.9410889365057145 1107 56-57 0.8918172359456236 1107 58-59 0.9593839900032748 1107 60-61 0.788577367933609 1107 62-63 1.0076011575322603 1107 64-65 0.9720917691360142 1107 66-67 1.1916979570010255 1107 68-69 1.149681754351331 1107 70-71 1.3312462173820343 1107 72-73 1.2184362257858865 1107 74-75 1.2369870589914385 1107 76-77 1.2444001501098612 1107 78-79 1.599658373497359 1107 80-81 1.658507084536712 1107 82-83 1.3864111502195726 1107 84-85 1.5780324637890146 1107 86-87 1.6904092999673068 1107 88-89 1.6720797015053002 1107 90-91 1.6593953120024842 1107 92-93 1.564400083372771 1107 94-95 1.7331410500612243 1107 96-97 1.8409344946146753 1107 98-99 1.7789805621255113 1107 100-101 1.7957274379922747 1108 1 0.3985383150034387 1108 2 0.27273677690391906 1108 3 0.18204781804472603 1108 4 0.257294371383864 1108 5 0.2506440559118843 1108 6 0.255245107422887 1108 7 0.3540129059364645 1108 8 0.402970638920344 1108 9 0.35797550325733596 1108 10-11 0.4941455098246337 1108 12-13 0.434477567383702 1108 14-15 0.481331378680828 1108 16-17 0.5275868605375251 1108 18-19 0.5921523877082535 1108 20-21 0.44961619444774215 1108 22-23 0.43008330637932346 1108 24-25 0.5378964819656318 1108 26-27 0.5733099536372279 1108 28-29 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1.5601625425502519 1108 96-97 1.6993973408845982 1108 98-99 1.9616828543472202 1108 100-101 1.6517813967458466 1109 1 0.3230128048305687 1109 2 0.2750832531258567 1109 3 0.31929832470006403 1109 4 0.39813144728123007 1109 5 0.3541341443438881 1109 6 0.4045010953068484 1109 7 0.41098115378727584 1109 8 0.36349696620877125 1109 9 0.5412816139708099 1109 10-11 0.5334153406074336 1109 12-13 0.5591884089777679 1109 14-15 0.6235153843648078 1109 16-17 0.534354063004777 1109 18-19 0.47632538374327993 1109 20-21 0.5103475434574634 1109 22-23 0.5885045342549304 1109 24-25 0.4140154277729522 1109 26-27 0.7155260758951911 1109 28-29 0.5706354870389205 1109 30-31 0.5466166117596032 1109 32-33 0.6453348970157435 1109 34-35 0.5430807588555666 1109 36-37 0.5098953584406232 1109 38-39 0.504207072884121 1109 40-41 0.7185532250736131 1109 42-43 0.8524778335691252 1109 44-45 0.6996188681815667 1109 46-47 0.7209128263545495 1109 48-49 0.8275686098900934 1109 50-51 0.7319614264399803 1109 52-53 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-1.2815730234756124 2316 54-55 -1.4637310565343746 2316 56-57 -1.4248208997488163 2316 58-59 -1.404568416413973 2316 60-61 -1.5507032285030107 2316 62-63 -1.5282091465857164 2316 64-65 -1.5210549933080415 2316 66-67 -1.599018203937387 2316 68-69 -1.6235560260215784 2316 70-71 -1.8842858014112593 2316 72-73 -1.7321773964893197 2316 74-75 -1.7548037728669783 2316 76-77 -1.9959217623217214 2316 78-79 -2.038806162323585 2316 80-81 -2.260082004713894 2316 82-83 -2.3948561254001994 2316 84-85 -2.33320087132428 2316 86-87 -2.3002953686129466 2316 88-89 -2.576178709649742 2316 90-91 -2.500336019622601 2316 92-93 -2.4600111815631003 2316 94-95 -2.872731041927171 2316 96-97 -2.9086230947400225 2316 98-99 -2.8148533169336076 2316 100-101 -2.4634886335611323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8814.0 3 2750.0 4 2834.0 5 3226.0 6 4183.0 7 5104.0 8 6583.0 9 8304.0 10 10503.0 11 13388.0 12 16785.0 13 19956.0 14 23016.0 15 27311.0 16 31644.0 17 37158.0 18 34650.0 19 31816.0 20 32305.0 21 35311.0 22 38997.0 23 44192.0 24 49622.0 25 55099.0 26 61542.0 27 70248.0 28 81127.0 29 93159.0 30 107254.0 31 126045.0 32 147348.0 33 178240.0 34 216201.0 35 263888.0 36 303218.0 37 283622.0 38 122624.0 39 5726.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.77319604259142 18.448561869364344 14.88341438065342 22.894827707390817 2 15.660090813670493 20.017070816977004 39.91437678061309 24.408461588739414 3 19.25700799100541 24.25767320317733 29.768398499773518 26.71692030604374 4 13.039943951266519 16.378488914455698 36.478896744359176 34.10267038991861 5 13.81892488356643 37.63815366576767 33.579837441158105 14.963084009507796 6 32.34320590308987 35.7755263503887 17.199430727345003 14.681837019176431 7 28.92522233533739 33.09340965405966 19.975759205547337 18.00560880505561 8 27.424835375998875 33.74299332360886 19.490920853321082 19.34125044707119 9 27.28243574545111 16.373126221111097 19.60861444658458 36.735823586853215 10-11 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28.608395894862042 22.31956435270675 36-37 23.090687263434972 25.558860260918014 28.655734240205966 22.694718235441044 38-39 22.32445403371921 26.120938581002477 28.556291655333187 22.998315729945123 40-41 22.86965700931797 25.86056956681686 28.76088624896384 22.508887174901332 42-43 22.58332395270936 26.00080372417815 28.443556625480287 22.972315697632204 44-45 22.54263060923257 26.17431259258861 28.65942255818607 22.62363423999275 46-47 23.06882428429912 26.49761256989345 27.73507764957496 22.698485496232465 48-49 22.54088231006969 26.73465229367471 27.629241795931346 23.09522360032425 50-51 22.92409718055928 26.776909054734926 27.557588323092254 22.74140544161354 52-53 23.263425306393355 26.349252318787364 27.660940051837592 22.726382322981692 54-55 22.562923867195625 26.77819928568781 27.810911803358845 22.84796504375772 56-57 22.702601171667112 26.951896656064445 27.43761054120127 22.907891631067173 58-59 22.66741829196033 26.304414005967548 27.64349144674104 23.384676255331083 60-61 22.212394275947986 26.67686212050842 28.009896581833882 23.100847021709708 62-63 22.38167275112882 26.588814742675165 28.15171714731325 22.87779535888276 64-65 22.198237742485173 26.734108292358606 27.832249646682367 23.235404318473858 66-67 22.130802955930864 26.727844297979864 27.73957403299352 23.401778713095755 68-69 22.835645240712616 26.233561412848506 27.89407093126963 23.036722415169248 70-71 22.610045284939755 26.398843625292333 27.50873283806432 23.482378251703587 72-73 22.532246672663547 26.473398884104064 27.603163325067936 23.391191118164457 74-75 22.490269802407166 26.977397819959364 27.394746557694088 23.137585819939385 76-77 22.600295641364283 26.42924698269868 27.549176883119475 23.42128049281756 78-79 22.658508102776704 26.788411397946227 27.451270709192443 23.10180979008463 80-81 22.907973735312915 26.748681081313602 27.13508123986038 23.208263943513103 82-83 22.811036234693688 26.78618855606427 27.2577429109854 23.14503229825664 84-85 22.694573888743154 26.915089618385874 27.234564079440755 23.155772413430217 86-87 22.91986077573566 26.8413864733214 26.90912415200665 23.329628598936285 88-89 22.742625257946443 26.86692518621514 26.774638855772448 23.615810700065968 90-91 22.57736335467561 26.773589363823397 27.046294108494074 23.602753173006917 92-93 22.857806927987692 26.41565429020637 27.189225477983513 23.537313303822426 94-95 22.622947229203703 26.221134216503316 27.40957895024013 23.746339604052842 96-97 22.949936890396774 25.73636515581444 27.39844348856823 23.915254465220556 98-99 22.658193905927845 26.0357990339931 26.981810442755084 24.32419661732397 100-101 23.313669235356326 25.397915004743865 27.407750192608482 23.880665567291324 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 544.0 1 382.5 2 314.5 3 408.5 4 377.5 5 377.5 6 611.5 7 1432.5 8 2358.0 9 2627.5 10 2317.0 11 1963.0 12 1932.5 13 2094.0 14 2326.5 15 2589.0 16 2836.5 17 3090.5 18 3411.5 19 3840.5 20 4355.0 21 5028.5 22 5959.5 23 7099.0 24 8582.5 25 10758.0 26 13908.5 27 17799.5 28 20841.5 29 23763.0 30 27766.0 31 34126.0 32 43390.5 33 47285.5 34 47431.5 35 50620.5 36 55596.5 37 60588.0 38 64363.5 39 67108.0 40 70098.0 41 74913.5 42 78845.5 43 81723.0 44 84230.5 45 88802.5 46 92363.5 47 93855.0 48 95151.5 49 94192.0 50 92739.0 51 91267.5 52 89744.5 53 90605.0 54 93021.0 55 89425.0 56 82565.5 57 75931.0 58 71366.5 59 65151.0 60 53796.0 61 45396.5 62 39135.5 63 29715.0 64 20857.5 65 15972.0 66 12188.0 67 9298.0 68 7247.5 69 5600.0 70 4401.5 71 3113.5 72 2291.5 73 1839.0 74 1369.0 75 1059.0 76 785.5 77 570.0 78 436.5 79 282.5 80 172.5 81 109.0 82 68.5 83 47.5 84 36.0 85 24.0 86 16.5 87 9.0 88 4.0 89 4.0 90 5.5 91 5.0 92 3.5 93 3.0 94 2.0 95 2.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006528936824087015 2 0.10995497721977131 3 0.11978678796663175 4 0.12285922882502565 5 0.16291617651633597 6 0.09904781217247301 7 0.028535294472333245 8 0.028420077940143475 9 0.0242338772705818 10-11 0.053690904000433214 12-13 0.05603364015495855 14-15 0.02891934957963248 16-17 0.03291352269554454 18-19 0.03840551072992361 20-21 0.06096874828375373 22-23 0.03362402464404813 24-25 0.05161700642101733 26-27 0.059317311322367026 28-29 0.05541915198327978 30-31 0.0711462086271835 32-33 0.05169381744247718 34-35 0.06482850211211105 36-37 0.05541915198327978 38-39 0.033988876995982395 40-41 0.03938485125353667 42-43 0.03494901476423049 44-45 0.007911535210364265 46-47 0.014056416927152045 48-49 0.009697391459305713 50-51 0.00871805093569266 52-53 0.010638326472188842 54-55 0.011886505570911358 56-57 0.015688651133173796 58-59 0.020950206103173333 60-61 0.025808503210508666 62-63 0.011656072506531818 64-65 0.009889419012955331 66-67 0.010811151270473498 68-69 0.01209773587992594 70-71 0.031895776661201566 72-73 0.02966825703886599 74-75 0.03126208573415782 76-77 0.02454112135642119 78-79 0.005703218343393657 80-81 0.005568799055838925 82-83 0.006509734068722053 84-85 0.005453582523649154 86-87 0.01411402519324693 88-89 0.03932724298744178 90-91 0.037560589493865294 92-93 0.030743611339303853 94-95 0.048928620669922686 96-97 0.04524169163985002 98-99 0.027114290575326074 100-101 0.07729109034397127 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2603793.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.98801797841714 #Duplication Level Percentage of deduplicated Percentage of total 1 72.2343969073099 33.21916743633487 2 13.643808224860788 12.549033958779468 3 5.53964702216528 7.642721605482655 4 2.7182676184323467 5.000309604264635 5 1.534094647450999 3.5274986113785025 6 0.9947848124378658 2.7448909103429333 7 0.6573658881058219 2.116166799642608 8 0.4494230264726682 1.6534459377071764 9 0.31865496578304237 1.3188879265808204 >10 1.6932694034994447 14.08214719492136 >50 0.13344589372226112 4.226332534214203 >100 0.07471016189710011 6.362025306698235 >500 0.0040237993546651234 1.3168375617056718 >1k 0.0038561410482186903 3.35292795989761 >5k 2.5148745966643637E-4 0.8876066520492862 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9992 0.38374786321339677 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7621 0.29268839727274787 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5411 0.20781221855961668 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3887 0.1492822202072131 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3811 0.1463634013917389 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3520 0.1351873977693311 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3321 0.12754470113407632 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3231 0.12408820516838319 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3041 0.11679115812969772 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2983 0.11456363850736215 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2837 0.10895643394079328 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.9202755364961806E-5 3.840551072992361E-5 14-15 0.0 0.0 0.0 3.840551072992361E-5 7.681102145984723E-5 16-17 0.0 0.0 0.0 3.840551072992361E-5 7.681102145984723E-5 18-19 0.0 0.0 0.0 7.681102145984723E-5 7.681102145984723E-5 20-21 0.0 0.0 0.0 1.7282479828465628E-4 7.681102145984723E-5 22-23 0.0 0.0 0.0 4.0325786266419796E-4 9.601377682480903E-5 24-25 0.0 0.0 0.0 7.873129699634341E-4 1.5362204291969445E-4 26-27 0.0 0.0 0.0 0.0016706397167516773 1.5362204291969445E-4 28-29 0.0 0.0 0.0 0.005223149459269612 1.5362204291969445E-4 30-31 0.0 0.0 0.0 0.015957489708283264 1.5362204291969445E-4 32-33 0.0 0.0 0.0 0.03771421153678499 2.688385751094653E-4 34-35 0.0 0.0 0.0 0.06970600197481136 2.688385751094653E-4 36-37 0.0 0.0 0.0 0.12226394340871183 2.688385751094653E-4 38-39 0.0 0.0 0.0 0.20502781903169723 3.072440858393889E-4 40-41 0.0 0.0 0.0 0.29432063147876963 3.456495965693125E-4 42-43 0.0 0.0 0.0 0.38920144573704596 3.456495965693125E-4 44-45 0.0 0.0 0.0 0.5057237652916342 3.456495965693125E-4 46-47 0.0 0.0 0.0 0.6457694601683006 3.8405510729923613E-4 48-49 0.0 0.0 0.0 0.7913839541008061 3.8405510729923613E-4 50-51 0.0 0.0 0.0 0.9518421779304269 3.8405510729923613E-4 52-53 0.0 0.0 0.0 1.1112442502149749 4.416633733941216E-4 54-55 0.0 0.0 0.0 1.2887737235640468 5.184743948539689E-4 56-57 0.0 0.0 0.0 1.4890008537545034 5.376771502189306E-4 58-59 0.0 0.0 0.0 1.7036300504686817 5.376771502189306E-4 60-61 0.0 0.0 0.0 1.9383645320499747 5.376771502189306E-4 62-63 0.0 0.0 0.0 2.1726957557686037 6.528936824087015E-4 64-65 0.0 0.0 0.0 2.419508770474458 6.720964377736633E-4 66-67 0.0 0.0 0.0 2.7046696876441407 6.91299193138625E-4 68-69 0.0 0.0 0.0 3.0229745605737475 6.91299193138625E-4 70-71 0.0 0.0 0.0 3.349997484439047 7.297047038685487E-4 72-73 0.0 0.0 0.0 3.6996412541242716 8.449212360583196E-4 74-75 0.0 0.0 0.0 4.055065053174349 8.641239914232814E-4 76-77 0.0 0.0 0.0 4.433474550396287 8.833267467882431E-4 78-79 0.0 0.0 0.0 4.8548598141250086 8.833267467882431E-4 80-81 0.0 0.0 0.0 5.2949101560684735 9.02529502153205E-4 82-83 0.0 0.0 0.0 5.763956658613031 9.409350128831285E-4 84-85 0.0 0.0 0.0 6.2413947652520765 9.601377682480904E-4 86-87 0.0 0.0 0.0 6.72493934809718 9.601377682480904E-4 88-89 0.0 0.0 0.0 7.229549353577646 9.601377682480904E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5115 0.0 58.578735 1 GTATCAA 9965 0.0 46.486485 1 TATCAAC 13465 0.0 34.15651 2 ATCAACG 13740 0.0 33.5075 3 TCAACGC 13780 0.0 33.341206 4 CAACGCA 14075 0.0 32.676197 5 AACGCAG 14680 0.0 31.431305 6 TAAGGTG 1845 0.0 28.356312 5 TGGTATC 2115 0.0 28.33437 2 ACGCAGA 16550 0.0 27.8251 7 CGCAGAG 16785 0.0 27.378906 8 GTGGTAT 2520 0.0 25.856222 1 GCAGAGT 18025 0.0 24.545792 9 AGGTAAG 1590 0.0 24.229324 2 GTAAGGT 1965 0.0 23.236038 4 TACATGG 12820 0.0 23.001547 2 GTGTAAG 1170 0.0 22.76393 1 GGTAAGG 1840 0.0 22.74669 3 GTACATG 13290 0.0 22.724472 1 AGTACTT 10165 0.0 22.252417 12-13 >>END_MODULE