FastQCFastQC Report
Thu 26 May 2016
SRR938571_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938571_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2603793
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94460.3627784543548585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78170.3002158773758129No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55900.214686804980273No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40280.15469739722013232No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37770.1450576140269215No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC37750.14498080300546165No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33150.12731426806969678No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG31840.12228314616407679No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31670.12163025248166809No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA30290.11633029200093864No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA26270.10089127668750934No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52650.062.1951181
GTATCAA99100.043.977561
TATCAAC135050.032.059682
ATCAACG136900.031.5089723
TCAACGC140300.030.745394
CAACGCA141900.030.398725
TGGTATC19700.029.915132
AACGCAG146600.029.4418336
AGGTAAG15750.026.2527082
ACGCAGA167450.025.7475347
CGCAGAG168300.025.5610718
TAAGGTG18400.025.044125
TACATGG128600.023.5414872
GTGGTAT26450.023.5386241
GTAAGGT19550.023.3279364
GTACATG133750.022.990371
AAGGTAA16850.022.8465461
GCAGAGT187750.022.7107459
GAGTACT104600.022.1298212-13
ACATGGG133800.021.9424043
Produced by FastQC (version 0.11.5)