##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938571_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2603793 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.787857176050476 31.0 31.0 34.0 30.0 34.0 2 32.00713343956298 31.0 31.0 34.0 30.0 34.0 3 32.06017605854229 33.0 31.0 34.0 30.0 34.0 4 35.750527403676095 37.0 35.0 37.0 35.0 37.0 5 35.644079617696185 37.0 35.0 37.0 33.0 37.0 6 35.64624684066667 37.0 35.0 37.0 33.0 37.0 7 35.58855177811754 37.0 35.0 37.0 33.0 37.0 8 35.56131689423852 37.0 35.0 37.0 33.0 37.0 9 37.13549425780006 39.0 37.0 39.0 33.0 39.0 10-11 37.04018387790427 39.0 37.0 39.0 33.0 39.0 12-13 36.93283145011911 39.0 37.0 39.0 33.0 39.0 14-15 37.96060228290037 40.0 37.0 41.0 33.0 41.0 16-17 37.878196730692494 40.0 37.0 41.0 33.0 41.0 18-19 38.12819605859605 40.0 37.0 41.0 33.0 41.0 20-21 38.10363669462204 40.0 37.0 41.0 33.0 41.0 22-23 37.99893559127012 40.0 37.0 41.0 33.0 41.0 24-25 37.868319985498076 40.0 37.0 41.0 32.0 41.0 26-27 37.66770918425543 40.0 37.0 41.0 32.0 41.0 28-29 37.551167085862815 39.5 36.5 41.0 32.0 41.0 30-31 37.27821105594799 39.0 36.0 41.0 31.0 41.0 32-33 37.05948591151447 39.0 36.0 41.0 31.0 41.0 34-35 36.95010087207393 39.0 36.0 41.0 31.0 41.0 36-37 36.88671680122037 39.0 35.0 41.0 31.0 41.0 38-39 36.70851811184683 39.0 35.0 41.0 30.0 41.0 40-41 36.52429878258371 39.0 35.0 40.5 30.0 41.0 42-43 36.28661840630188 39.0 35.0 40.0 30.0 41.0 44-45 35.952356235691546 38.0 35.0 40.0 29.0 41.0 46-47 35.52986354906093 38.0 34.5 40.0 27.5 41.0 48-49 35.134466718360486 38.0 34.0 40.0 26.5 41.0 50-51 34.74707205987573 38.0 34.0 40.0 25.5 41.0 52-53 34.24363975938179 38.0 33.0 40.0 24.0 41.0 54-55 33.894206259867815 37.0 33.0 40.0 23.0 41.0 56-57 33.559101088296956 37.0 32.5 40.0 23.0 41.0 58-59 33.073194374514415 36.0 32.0 39.0 21.5 40.5 60-61 32.67384926528338 36.0 31.0 39.0 20.5 40.0 62-63 32.19472554077839 35.0 31.0 39.0 18.5 40.0 64-65 31.67433759135231 35.0 30.5 38.0 17.5 40.0 66-67 31.178903814550544 35.0 30.0 38.0 13.5 40.0 68-69 30.68322347436989 34.0 29.0 37.0 10.0 39.5 70-71 30.12337309455859 34.0 29.0 36.5 7.5 39.0 72-73 29.6346253331198 34.0 28.0 36.0 7.0 39.0 74-75 29.06364350007854 33.5 27.5 35.5 4.0 38.0 76-77 27.854076725761225 31.5 26.0 34.5 2.0 36.0 78-79 28.55286096091356 33.0 27.0 35.0 2.0 37.0 80-81 28.202092869901715 33.0 26.5 35.0 2.0 36.5 82-83 27.772412399910436 33.0 26.0 35.0 2.0 36.0 84-85 27.23732397314226 32.5 26.0 35.0 2.0 36.0 86-87 26.658588643567285 32.0 25.0 34.5 2.0 35.0 88-89 25.920633667883735 31.5 23.5 34.0 2.0 35.0 90-91 25.323099800944235 31.0 21.0 34.0 2.0 35.0 92-93 24.51401801141642 31.0 18.5 34.0 2.0 35.0 94-95 23.717805524479097 31.0 9.5 34.0 2.0 35.0 96-97 22.65501827526228 30.0 2.0 34.0 2.0 35.0 98-99 21.287999852522837 29.5 2.0 34.0 2.0 35.0 100-101 17.361983268255194 22.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33343972807502453 1101 2 0.31987412778183 1101 3 0.3249450514454466 1101 4 0.25536794218902514 1101 5 0.10637317510700939 1101 6 0.26028956718262464 1101 7 0.22636092995915646 1101 8 0.2934553592302436 1101 9 0.29798945914541264 1101 10-11 0.27202866934650416 1101 12-13 0.3239498565508896 1101 14-15 0.48832317279307347 1101 16-17 0.4132484795315037 1101 18-19 0.5084644286501998 1101 20-21 0.45210707322013377 1101 22-23 0.5434501773019136 1101 24-25 0.5013223204226023 1101 26-27 0.4236980018801404 1101 28-29 0.4341612332086484 1101 30-31 0.39630831878196915 1101 32-33 0.3541131167993683 1101 34-35 0.42679735344585623 1101 36-37 0.6416855898650553 1101 38-39 0.7167101238822937 1101 40-41 0.6057905428013655 1101 42-43 0.6166942892552711 1101 44-45 0.6484003428698273 1101 46-47 0.4911382997296343 1101 48-49 0.48732571580256945 1101 50-51 0.5704496609625664 1101 52-53 0.5830229070381634 1101 54-55 0.6089962330672449 1101 56-57 0.6834689629278969 1101 58-59 0.8360194326813897 1101 60-61 0.7761759546669253 1101 62-63 0.9486750453893222 1101 64-65 0.6661806311561378 1101 66-67 0.6039544799592953 1101 68-69 0.6333593161857145 1101 70-71 0.5982573000668054 1101 72-73 0.5985669656829771 1101 74-75 0.5669069288099671 1101 76-77 0.6313179628781214 1101 78-79 0.6626067126527566 1101 80-81 0.779406494090491 1101 82-83 0.6199100674328228 1101 84-85 0.9707982164935558 1101 86-87 1.0624848471647148 1101 88-89 1.0344954969326245 1101 90-91 1.0340711859867895 1101 92-93 1.3629321649671304 1101 94-95 1.2473260955022418 1101 96-97 1.496808133019691 1101 98-99 1.6971256677705746 1101 100-101 1.7741914500671747 1102 1 0.3091499087226417 1102 2 0.2806215186513654 1102 3 0.2675528190432743 1102 4 0.25882166887384983 1102 5 0.12508626001388023 1102 6 0.21125451835920472 1102 7 0.14297432947812183 1102 8 0.2574880374256452 1102 9 0.2290286580075076 1102 10-11 0.39610993101968717 1102 12-13 0.34294391366479005 1102 14-15 0.5235078034453835 1102 16-17 0.48592358448218675 1102 18-19 0.46273266281066583 1102 20-21 0.43139934957969217 1102 22-23 0.445585230156361 1102 24-25 0.4266422592277621 1102 26-27 0.30930152346245166 1102 28-29 0.36129801364626957 1102 30-31 0.4565273091439792 1102 32-33 0.5042117809936713 1102 34-35 0.6479399961490273 1102 36-37 0.6931128614929563 1102 38-39 0.5245356917292838 1102 40-41 0.418561797353064 1102 42-43 0.3709748564400144 1102 44-45 0.43924232851553313 1102 46-47 0.42747350077232227 1102 48-49 0.6371148546791474 1102 50-51 0.6507817984244397 1102 52-53 0.6761523567645327 1102 54-55 0.5198404645710681 1102 56-57 0.5658999699745166 1102 58-59 0.5903705428455268 1102 60-61 0.8209381144530141 1102 62-63 0.6845599710744565 1102 64-65 0.7939493231261423 1102 66-67 0.7447990569797547 1102 68-69 0.7623905835215332 1102 70-71 0.6953671494023581 1102 72-73 0.9403097899743287 1102 74-75 0.9879103091143691 1102 76-77 0.8698959987696213 1102 78-79 0.9557209500495603 1102 80-81 0.8390625802700171 1102 82-83 0.9570556009584905 1102 84-85 1.0764308094615274 1102 86-87 0.9976241767472764 1102 88-89 0.9204224604748603 1102 90-91 1.0554980161212129 1102 92-93 1.428308710914127 1102 94-95 1.557489179353368 1102 96-97 1.5038024454319903 1102 98-99 1.7386373318362693 1102 100-101 1.7502109160904133 1103 1 0.23309854186247847 1103 2 0.2433339286095162 1103 3 0.24887295178909596 1103 4 0.21579859374371324 1103 5 0.11464103616255272 1103 6 0.1430474748394417 1103 7 0.22461041799013515 1103 8 0.2080468795624597 1103 9 0.32657815096139586 1103 10-11 0.25553088843225424 1103 12-13 0.33733005674761785 1103 14-15 0.5931466371468375 1103 16-17 0.4625108552385129 1103 18-19 0.4256221966834204 1103 20-21 0.40895085887892435 1103 22-23 0.3804429390700861 1103 24-25 0.48207048288301024 1103 26-27 0.5691534053231138 1103 28-29 0.4524100003568847 1103 30-31 0.35238260954243117 1103 32-33 0.4020024954458705 1103 34-35 0.482202883185586 1103 36-37 0.48140584942078846 1103 38-39 0.3915799144374006 1103 40-41 0.31328359719101684 1103 42-43 0.5211028666216322 1103 44-45 0.5542359835021955 1103 46-47 0.6399398796010729 1103 48-49 0.6267954211796933 1103 50-51 0.8067128135155812 1103 52-53 0.6828224826658769 1103 54-55 0.6205661639099276 1103 56-57 0.6389032331242319 1103 58-59 0.6767135953334034 1103 60-61 0.6397213887758681 1103 62-63 0.7035926987642398 1103 64-65 0.6912599993972748 1103 66-67 0.7190277586383367 1103 68-69 0.6869339460456239 1103 70-71 0.6032821373227861 1103 72-73 0.5385520547926177 1103 74-75 0.4454690143616773 1103 76-77 0.3885661542686947 1103 78-79 0.6152036927231954 1103 80-81 0.6574319488394487 1103 82-83 0.6673474715720182 1103 84-85 0.6665216744921771 1103 86-87 0.5882172782563906 1103 88-89 0.4752166452608897 1103 90-91 0.7066360488341061 1103 92-93 1.0436603134462104 1103 94-95 0.6571123632551625 1103 96-97 0.8793310423885998 1103 98-99 1.226350111766049 1103 100-101 1.4834442645925847 1104 1 0.2747505696737811 1104 2 0.24122904651049026 1104 3 0.3073598856285855 1104 4 0.19320909808229914 1104 5 0.1047187829380718 1104 6 0.06363594644806625 1104 7 0.09756753792913031 1104 8 0.2506328292854363 1104 9 0.3174082237213298 1104 10-11 0.3300950656188846 1104 12-13 0.28646014554728083 1104 14-15 0.4044026494544468 1104 16-17 0.4927684427610828 1104 18-19 0.40797537155837205 1104 20-21 0.4397888121608915 1104 22-23 0.3771860717050899 1104 24-25 0.41267489639042765 1104 26-27 0.43956855288426055 1104 28-29 0.3769369540844636 1104 30-31 0.37279910610189404 1104 32-33 0.4562578735760283 1104 34-35 0.5181201857344817 1104 36-37 0.5026238140461956 1104 38-39 0.549407158451622 1104 40-41 0.5148335073121544 1104 42-43 0.5199611100242194 1104 44-45 0.42354398887328415 1104 46-47 0.36063244644245174 1104 48-49 0.4746667314839925 1104 50-51 0.4889008176295775 1104 52-53 0.3011868563022446 1104 54-55 0.4857030220127996 1104 56-57 0.4065123307688765 1104 58-59 0.511206103649009 1104 60-61 0.5565755069119547 1104 62-63 0.46586072664432976 1104 64-65 0.6373776711862789 1104 66-67 0.48730275743016804 1104 68-69 0.6880797792863085 1104 70-71 0.7179509967788036 1104 72-73 0.5717614285223753 1104 74-75 0.7230135587308233 1104 76-77 0.4647450527922956 1104 78-79 0.6886564287141432 1104 80-81 0.7421629037256814 1104 82-83 0.9861168115382704 1104 84-85 1.0756470699636367 1104 86-87 1.0297364964421938 1104 88-89 1.137021433048627 1104 90-91 1.126472565062258 1104 92-93 1.3433816489367558 1104 94-95 1.3393817519928923 1104 96-97 1.4666588931555893 1104 98-99 1.6706101684002554 1104 100-101 1.833214178846692 1105 1 0.23952041156922022 1105 2 0.25782255734279147 1105 3 0.19904021324982324 1105 4 0.15919785261212382 1105 5 0.1348029518412872 1105 6 0.14919209167163672 1105 7 0.12982762395696312 1105 8 0.18068115442922306 1105 9 0.2504323817969407 1105 10-11 0.24996417056044606 1105 12-13 0.2544195340479689 1105 14-15 0.3345931693631883 1105 16-17 0.2406015533179371 1105 18-19 0.2941462843265157 1105 20-21 0.11701744462241237 1105 22-23 0.1986932828203365 1105 24-25 0.3236975760339078 1105 26-27 0.18785164242804342 1105 28-29 0.419879140812327 1105 30-31 0.36482671492971974 1105 32-33 0.3839368714233018 1105 34-35 0.46798240996691476 1105 36-37 0.5284283701519357 1105 38-39 0.49525104574176027 1105 40-41 0.5216436810946661 1105 42-43 0.39616145795176294 1105 44-45 0.3884093064038936 1105 46-47 0.48042633709776794 1105 48-49 0.6416472944993856 1105 50-51 0.7158661438857692 1105 52-53 0.7996286855058159 1105 54-55 0.7677456357605266 1105 56-57 0.6447922574627043 1105 58-59 0.6606820976740195 1105 60-61 0.6822476476555437 1105 62-63 0.5691114085679843 1105 64-65 0.6848249168582257 1105 66-67 0.5130015807684885 1105 68-69 0.5507466842556674 1105 70-71 0.6443666812548052 1105 72-73 0.721875121105441 1105 74-75 0.7442228493321004 1105 76-77 0.5746121470549213 1105 78-79 0.8621361495908779 1105 80-81 0.8414951503650308 1105 82-83 0.8114678478388235 1105 84-85 0.7698577197051897 1105 86-87 0.7965946904646977 1105 88-89 0.9174744020598631 1105 90-91 0.7707696206049093 1105 92-93 1.062835382174601 1105 94-95 1.2313468204156948 1105 96-97 1.1225387801697657 1105 98-99 1.3443586823455327 1105 100-101 1.3862264105471631 1106 1 -0.013877388934957935 1106 2 0.054161964117128036 1106 3 0.07194350655098702 1106 4 0.04586385315856489 1106 5 -0.00678145519631812 1106 6 -0.07505147836793213 1106 7 0.06430405829554076 1106 8 0.12292327918137858 1106 9 -0.08458532359576765 1106 10-11 0.07069953965116582 1106 12-13 0.10330387249729966 1106 14-15 0.010061536875262789 1106 16-17 0.1185024067119187 1106 18-19 0.09106453852214713 1106 20-21 0.24129986246193624 1106 22-23 0.142731168200946 1106 24-25 0.19000959586936972 1106 26-27 0.1148970357575223 1106 28-29 0.13837673289825858 1106 30-31 0.09195609192349963 1106 32-33 0.11926933935313144 1106 34-35 0.13424705511071267 1106 36-37 0.2067221515602995 1106 38-39 0.10280437923817232 1106 40-41 0.1421665965287744 1106 42-43 0.2149675659702055 1106 44-45 0.0875301244437523 1106 46-47 -0.6186710008950911 1106 48-49 -0.31987762883114357 1106 50-51 0.13374547413366855 1106 52-53 0.21145786132299094 1106 54-55 0.3554286858061957 1106 56-57 0.23820083197784925 1106 58-59 0.02678046151967095 1106 60-61 0.3220562638515858 1106 62-63 0.13382491951771414 1106 64-65 0.25727198690353603 1106 66-67 0.32020959588085063 1106 68-69 -0.029340038517119638 1106 70-71 0.09819791596392236 1106 72-73 0.4465483525113285 1106 74-75 0.3806818620009622 1106 76-77 0.306650774841728 1106 78-79 0.5642223082344593 1106 80-81 0.01327546446697525 1106 82-83 0.552078973069289 1106 84-85 0.682119459072247 1106 86-87 0.825066976891975 1106 88-89 0.9519626386115938 1106 90-91 1.067187317235792 1106 92-93 1.070283145288979 1106 94-95 1.1287631998698373 1106 96-97 1.419847999950882 1106 98-99 1.1916372149904682 1106 100-101 1.167548192084329 1107 1 0.18685684516956158 1107 2 0.23477019625126871 1107 3 0.22451799701282482 1107 4 0.18327573607534475 1107 5 0.1868824565376812 1107 6 0.2299850176962437 1107 7 0.2105703383495836 1107 8 0.2108193810266883 1107 9 0.30781905514778174 1107 10-11 0.22388444975318578 1107 12-13 0.2891810468722227 1107 14-15 0.3958180198630785 1107 16-17 0.3756911288609288 1107 18-19 0.3739367853301445 1107 20-21 0.30859791800160963 1107 22-23 0.42888670204367685 1107 24-25 0.3867416943839288 1107 26-27 0.39044593686021045 1107 28-29 0.4500430757653646 1107 30-31 0.4067610598065059 1107 32-33 0.4375004311146853 1107 34-35 0.4922533069434891 1107 36-37 0.6088099624873067 1107 38-39 0.5403077507419951 1107 40-41 0.6155161347121734 1107 42-43 0.5057324778865819 1107 44-45 0.4697619917018798 1107 46-47 0.5918995744987683 1107 48-49 0.6718142421503472 1107 50-51 0.7034106464552039 1107 52-53 0.5534358318662882 1107 54-55 0.4245477252071268 1107 56-57 0.4813232683674613 1107 58-59 0.45174366396361876 1107 60-61 0.4776754166161652 1107 62-63 0.6879860413703227 1107 64-65 0.5088811490657363 1107 66-67 0.49648175299840247 1107 68-69 0.8752341339586742 1107 70-71 0.8335510555556098 1107 72-73 0.729656317291127 1107 74-75 0.7292579753813762 1107 76-77 0.7578850054986894 1107 78-79 0.9639474639185082 1107 80-81 1.0974411114016114 1107 82-83 1.3124706745528592 1107 84-85 1.4146351275236633 1107 86-87 1.5422555016516917 1107 88-89 1.503772732262 1107 90-91 1.5610392490348168 1107 92-93 1.7079638697239226 1107 94-95 1.8147842926737674 1107 96-97 1.7016500182158794 1107 98-99 1.924415505845218 1107 100-101 1.9817482158521607 1108 1 0.13046551144570628 1108 2 0.06435764209521011 1108 3 0.13045618864376962 1108 4 0.127916666003145 1108 5 0.0014614343066341462 1108 6 0.15364162479635723 1108 7 0.10581743212095773 1108 8 0.07930645914457557 1108 9 0.09263722233446003 1108 10-11 0.14656639081734824 1108 12-13 0.14835875716683944 1108 14-15 0.3264580314969763 1108 16-17 0.24772556651620903 1108 18-19 0.26114370129236875 1108 20-21 0.22362856728605607 1108 22-23 0.2120422997557867 1108 24-25 0.2260139137875541 1108 26-27 0.19722860757586602 1108 28-29 0.29553481643066704 1108 30-31 0.20741571185563146 1108 32-33 0.27290523614470885 1108 34-35 0.1789792623899018 1108 36-37 0.28056503128173915 1108 38-39 0.3829038836135723 1108 40-41 0.4366071152523503 1108 42-43 0.4991304022482481 1108 44-45 0.5375033236692133 1108 46-47 0.5038930729125894 1108 48-49 0.48856661080122876 1108 50-51 0.4341452652366016 1108 52-53 0.42723332845333317 1108 54-55 0.42343294582233426 1108 56-57 0.4806645722304239 1108 58-59 0.42313419295482646 1108 60-61 0.307407469239962 1108 62-63 0.5800828315082995 1108 64-65 0.6186001135504711 1108 66-67 0.39434402361896304 1108 68-69 0.4180175203553418 1108 70-71 0.5129098266103966 1108 72-73 0.4105980015434483 1108 74-75 0.7104139554436131 1108 76-77 0.7623332979540542 1108 78-79 0.8379249019929027 1108 80-81 0.8870617351061725 1108 82-83 0.8529640451081413 1108 84-85 1.0089416236360265 1108 86-87 1.1273149252343622 1108 88-89 1.1891234935717598 1108 90-91 1.140639358184341 1108 92-93 1.494459720653122 1108 94-95 1.630696593972207 1108 96-97 1.7912508708239905 1108 98-99 2.0288873683887125 1108 100-101 1.9263147999213963 1109 1 0.2368176891242051 1109 2 0.2626182783616784 1109 3 0.25370911256288053 1109 4 0.18627706902027086 1109 5 0.16740405793796498 1109 6 0.1273331256584811 1109 7 0.23583449978598736 1109 8 0.2145395206012779 1109 9 0.24806352506572438 1109 10-11 0.26994734407221443 1109 12-13 0.3457504980948869 1109 14-15 0.4751938182743842 1109 16-17 0.48934139286473766 1109 18-19 0.4132239813663716 1109 20-21 0.42569971489207603 1109 22-23 0.477226447888782 1109 24-25 0.2672199151735839 1109 26-27 0.4361923040493352 1109 28-29 0.44245525209745296 1109 30-31 0.6003167692119007 1109 32-33 0.5026003515415312 1109 34-35 0.6040176493426159 1109 36-37 0.642563608539362 1109 38-39 0.6514711960200046 1109 40-41 0.6378886757944926 1109 42-43 0.580834529512039 1109 44-45 0.5589261709192925 1109 46-47 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-0.7291141706426743 2316 74-75 -0.5135898051746928 2316 76-77 -0.5183170551804466 2316 78-79 -0.7693803618537309 2316 80-81 -0.8881591314986004 2316 82-83 -0.8055677101813679 2316 84-85 -0.9979727761747625 2316 86-87 -1.221925952236095 2316 88-89 -1.2680206353962085 2316 90-91 -1.2379554727353224 2316 92-93 -1.4199635537770874 2316 94-95 -1.1717662302349758 2316 96-97 -1.2102558971626962 2316 98-99 -1.035732839732443 2316 100-101 -1.6993612881345648 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 18.0 4 46.0 5 102.0 6 217.0 7 602.0 8 1416.0 9 2298.0 10 3938.0 11 6008.0 12 8348.0 13 11381.0 14 14883.0 15 19838.0 16 24962.0 17 31336.0 18 28845.0 19 23914.0 20 23705.0 21 24671.0 22 27236.0 23 30608.0 24 34940.0 25 41004.0 26 48668.0 27 58737.0 28 69991.0 29 83847.0 30 98651.0 31 117482.0 32 141288.0 33 174281.0 34 222950.0 35 289265.0 36 370769.0 37 390479.0 38 170984.0 39 6079.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.40158077567848 19.315112422876695 15.485303126026151 22.79800367541867 2 15.960968876852302 20.704908785461008 38.93786437015166 24.396257967535032 3 19.13642904793123 24.492653601880026 30.276830761892366 26.094086588296385 4 13.436782417035456 17.13945770650739 36.077714319072214 33.34604555738494 5 13.742448728973441 37.87723694756895 33.676344128163926 14.703970195293678 6 32.27385290376497 36.21460546771785 16.892785598849677 14.618756029667502 7 28.84979719970059 33.20632630934948 19.85031068137905 18.093565809570883 8 27.391847201371228 34.24642435093727 19.27645554005253 19.085272907638974 9 27.004297192595573 16.38709375130819 19.560233858835936 37.048375197260306 10-11 25.263413796718865 26.57880253921875 26.73517057615563 21.422613087906758 12-13 26.336559780289754 23.868583255274135 27.19025667554986 22.60460028888625 14-15 22.97813228624549 25.21496524493306 25.687564257220142 26.119338211601306 16-17 22.253477907037926 28.4242833435684 26.586790885450572 22.7354478639431 18-19 22.41881363073025 26.464258103466747 29.27588713849373 21.841041127309275 20-21 23.05321505972249 25.523207874051433 29.431851917567947 21.99172514865813 22-23 22.783435549600142 25.514048159742348 29.501845960873236 22.20067032978428 24-25 22.519580854020017 25.88962898878584 29.44849061549703 22.142299541697113 26-27 21.910819854970214 26.226072408891664 29.293735436819563 22.56937229931856 28-29 22.186347173476513 26.606806499985307 29.055390593060388 22.15145573347779 30-31 22.274271023403525 26.119278595166286 28.902645527436743 22.703804853993446 32-33 21.86504512763456 26.14551182578108 28.87840243123319 23.111040615351172 34-35 22.778621204220908 26.212368696747703 28.679712729273554 22.329297369757832 36-37 23.012596823289524 25.637812135544152 28.74078632573191 22.608804715434413 38-39 22.275798286342628 26.136539524386915 28.666237016888847 22.92142517238161 40-41 22.78407944186986 25.937973292909827 28.80998692086519 22.467960344355127 42-43 22.527178995582155 26.085748401252495 28.49432569936442 22.89274690380093 44-45 22.479186827212306 26.22833295468796 28.73380666885724 22.558673549242496 46-47 23.054309616778294 26.54239411504678 27.7909572688766 22.61233899929833 48-49 22.504592338945532 26.78876546637924 27.61584734270351 23.09079485197172 50-51 22.88614574077961 26.82311354586149 27.577094152137633 22.71364656122127 52-53 23.26337001443663 26.423682681380583 27.607647766162668 22.705299538020114 54-55 22.55593891208295 26.79099477658329 27.77712807519754 22.87593823613623 56-57 22.705395551796936 27.00220409226079 27.346413482177727 22.945986873764543 58-59 22.59259024503333 26.457502977612158 27.569509789290127 23.38039698806439 60-61 22.18980157024771 26.810695013006026 27.898319874122095 23.101183542624167 62-63 22.358670690408125 26.7434196501926 28.015261487501476 22.8826481718978 64-65 22.129046234947918 26.82959479168787 27.6550418936635 23.38631707970071 66-67 22.06237853450196 26.855858381791503 27.5738010923253 23.507961991381233 68-69 22.784323170305427 26.400076362912824 27.651767448042108 23.163833018739645 70-71 22.525248277367833 26.533441713747045 27.335483659117866 23.605826349767252 72-73 22.3969161893339 26.62642150878824 27.33026050664963 23.64640179522823 74-75 22.38128439309689 27.066428495971266 27.101020868746815 23.451266242185024 76-77 22.413726853159375 26.584817217241678 27.49664644350098 23.504809486097965 78-79 22.436282106741636 26.8560806590041 27.3878602728127 23.31977696144156 80-81 22.68808649475946 26.811139611635642 27.045881576030773 23.454892317574128 82-83 22.605074286645156 26.866598316921138 27.17480610706321 23.353521289370498 84-85 22.412952016295844 27.01332852929077 27.079685007430772 23.494034446982614 86-87 22.65855051110386 26.905147873186863 26.789343628068263 23.646957987641017 88-89 22.426150010311062 26.96503598109529 26.5652396270028 24.04357438159084 90-91 22.22305455643437 26.8833450308303 26.93011581098228 23.96348460175305 92-93 22.405659652541097 26.552862556124783 27.013250981127317 24.0282268102068 94-95 22.0994809421065 26.349002134585493 27.264743727770334 24.286773195537673 96-97 22.437587827525228 25.88126246874709 27.198154265236003 24.482995438491674 98-99 22.031298964072725 26.294880270923887 26.808242305189395 24.86557845981399 100-101 22.171077889381127 25.92975422872214 27.312802235063042 24.586365646833688 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 586.0 1 403.0 2 273.5 3 366.0 4 362.5 5 379.0 6 643.5 7 1464.0 8 2314.0 9 2537.5 10 2221.0 11 1883.5 12 1908.0 13 2068.5 14 2287.5 15 2533.5 16 2765.5 17 3082.5 18 3453.0 19 3795.5 20 4328.5 21 5012.0 22 5885.5 23 7091.0 24 8601.5 25 10605.0 26 13735.0 27 17607.0 28 20682.0 29 23893.5 30 27972.5 31 34258.0 32 44211.0 33 48292.5 34 48020.5 35 50895.0 36 55406.0 37 60414.0 38 64333.5 39 66913.5 40 70070.5 41 75188.5 42 78220.5 43 80783.5 44 84303.0 45 89119.5 46 92289.0 47 94065.5 48 95526.0 49 94676.5 50 93753.5 51 92364.0 52 90974.0 53 92307.0 54 95561.0 55 91829.0 56 83782.0 57 76218.5 58 70977.5 59 64548.0 60 53241.0 61 45181.5 62 38570.5 63 28266.5 64 19292.5 65 14383.5 66 10761.0 67 8401.5 68 6599.0 69 5054.5 70 3916.5 71 2659.0 72 1932.5 73 1668.0 74 1247.5 75 897.5 76 643.5 77 462.5 78 351.5 79 215.0 80 116.0 81 74.5 82 52.0 83 27.5 84 10.5 85 6.0 86 5.5 87 2.0 88 0.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.072440858393889E-4 2 0.052116278060506345 3 0.0 4 0.0 5 0.04804529392313444 6 3.840551072992361E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006356112025802359 26-27 0.001997086557956028 28-29 9.601377682480903E-5 30-31 0.0015362204291969445 32-33 4.416633733941216E-4 34-35 0.012500993742590137 36-37 0.0229280899057644 38-39 0.004685472309050681 40-41 0.0010561515450728993 42-43 0.00138259838627725 44-45 0.008199576540838692 46-47 0.0 48-49 0.0 50-51 4.800688841240452E-4 52-53 0.0 54-55 0.0014402066523721356 56-57 0.0 58-59 0.02367699736499791 60-61 0.0 62-63 0.009447755639561208 64-65 0.015976692463648225 66-67 0.006644153356276786 68-69 0.016706397167516773 70-71 5.760826609488542E-5 72-73 5.760826609488542E-5 74-75 0.023734605631092795 76-77 0.051156140292258255 78-79 0.03206860145948622 80-81 0.02772877874700485 82-83 0.03853993001747835 84-85 0.016514369613867156 86-87 0.010043041055875026 88-89 0.08520262555433554 90-91 0.025808503210508666 92-93 0.025539664635399206 94-95 0.027325520884340652 96-97 0.0397689063608359 98-99 0.05524632718499512 100-101 0.01445967478981624 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2603793.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.22345840601755 #Duplication Level Percentage of deduplicated Percentage of total 1 69.36630626941185 29.982516538149973 2 15.03457777949095 12.996928946077258 3 6.0380274818845745 7.829532891528862 4 3.022457169047004 5.225642069210909 5 1.7321042656192016 3.7433768339938562 6 1.0777608534407783 2.7950730852198875 7 0.7010729473798019 2.1211958166458498 8 0.49122469674817937 1.6985944198322813 9 0.3775455436299673 1.4686941691320459 >10 1.911003381228022 14.867525773310152 >50 0.1528085516885592 4.5357376130458915 >100 0.0857969603111557 6.893712173686066 >500 0.004523991486860864 1.3377028041624623 >1k 0.00452399148685663 3.6275444983956517 >5k 2.6611714628568415E-4 0.8762223676087965 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9446 0.3627784543548585 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7817 0.3002158773758129 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5590 0.214686804980273 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4028 0.15469739722013232 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3777 0.1450576140269215 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3775 0.14498080300546165 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3315 0.12731426806969678 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3184 0.12228314616407679 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3167 0.12163025248166809 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3029 0.11633029200093864 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2627 0.10089127668750934 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.9202755364961806E-5 3.840551072992361E-5 14-15 0.0 0.0 0.0 3.840551072992361E-5 9.601377682480903E-5 16-17 0.0 0.0 0.0 3.840551072992361E-5 1.1521653218977085E-4 18-19 0.0 0.0 0.0 7.681102145984723E-5 1.1521653218977085E-4 20-21 0.0 0.0 0.0 1.7282479828465628E-4 1.1521653218977085E-4 22-23 0.0 0.0 0.0 4.0325786266419796E-4 1.1521653218977085E-4 24-25 0.0 0.0 0.0 7.873129699634341E-4 1.1521653218977085E-4 26-27 0.0 0.0 0.0 0.0015746259399268682 1.1521653218977085E-4 28-29 0.0 0.0 0.0 0.0051463384378097644 1.1521653218977085E-4 30-31 0.0 0.0 0.0 0.016015097974378147 1.1521653218977085E-4 32-33 0.0 0.0 0.0 0.03782942806897476 1.5362204291969445E-4 34-35 0.0 0.0 0.0 0.06997484054992083 1.5362204291969445E-4 36-37 0.0 0.0 0.0 0.12266720127137604 1.9202755364961806E-4 38-39 0.0 0.0 0.0 0.20552709067118624 2.304330643795417E-4 40-41 0.0 0.0 0.0 0.29562641884358704 2.688385751094653E-4 42-43 0.0 0.0 0.0 0.3908144771877027 2.688385751094653E-4 44-45 0.0 0.0 0.0 0.5075288242959406 2.688385751094653E-4 46-47 0.0 0.0 0.0 0.648073790812096 2.688385751094653E-4 48-49 0.0 0.0 0.0 0.7944755977145649 2.688385751094653E-4 50-51 0.0 0.0 0.0 0.9551834573639302 2.688385751094653E-4 52-53 0.0 0.0 0.0 1.1149311792450476 2.688385751094653E-4 54-55 0.0 0.0 0.0 1.2929791269889734 2.688385751094653E-4 56-57 0.0 0.0 0.0 1.4941279894369484 2.688385751094653E-4 58-59 0.0 0.0 0.0 1.709448485344265 2.688385751094653E-4 60-61 0.0 0.0 0.0 1.9449894826508867 3.456495965693125E-4 62-63 0.0 0.0 0.0 2.180108019339479 3.456495965693125E-4 64-65 0.0 0.0 0.0 2.4280155911011363 3.456495965693125E-4 66-67 0.0 0.0 0.0 2.714655120433921 3.456495965693125E-4 68-69 0.0 0.0 0.0 3.0350146881875784 3.456495965693125E-4 70-71 0.0 0.0 0.0 3.365206066688097 3.648523519342743E-4 72-73 0.0 0.0 0.0 3.7194969031716423 5.184743948539689E-4 74-75 0.0 0.0 0.0 4.078972483603727 5.376771502189306E-4 76-77 0.0 0.0 0.0 4.463776498362197 5.568799055838924E-4 78-79 0.0 0.0 0.0 4.894897559060954 5.760826609488542E-4 80-81 0.0 0.0 0.0 5.346507959734126 5.95285416313816E-4 82-83 0.0 0.0 0.0 5.828458713883938 6.144881716787778E-4 84-85 0.0 0.0 0.0 6.319127518969442 6.144881716787778E-4 86-87 0.0 0.0 0.0 6.819647337557171 6.144881716787778E-4 88-89 0.0 0.0 0.0 7.344209005861833 6.144881716787778E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5265 0.0 62.195118 1 GTATCAA 9910 0.0 43.97756 1 TATCAAC 13505 0.0 32.05968 2 ATCAACG 13690 0.0 31.508972 3 TCAACGC 14030 0.0 30.74539 4 CAACGCA 14190 0.0 30.39872 5 TGGTATC 1970 0.0 29.91513 2 AACGCAG 14660 0.0 29.441833 6 AGGTAAG 1575 0.0 26.252708 2 ACGCAGA 16745 0.0 25.747534 7 CGCAGAG 16830 0.0 25.561071 8 TAAGGTG 1840 0.0 25.04412 5 TACATGG 12860 0.0 23.541487 2 GTGGTAT 2645 0.0 23.538624 1 GTAAGGT 1955 0.0 23.327936 4 GTACATG 13375 0.0 22.99037 1 AAGGTAA 1685 0.0 22.846546 1 GCAGAGT 18775 0.0 22.710745 9 GAGTACT 10460 0.0 22.12982 12-13 ACATGGG 13380 0.0 21.942404 3 >>END_MODULE