FastQCFastQC Report
Thu 26 May 2016
SRR938570_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938570_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2587360
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99150.38320913981819305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76390.29524302764207533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54950.21237864077669902No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA38590.14914816647084286No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA37690.14566971739533732No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35660.13782388225836376No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA33440.12924370787211675No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC32370.12510821841568237No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA30520.1179580730938099No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA29520.11409312967658153No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27910.10787057077484385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48050.059.7532041
GTATCAA96950.047.196841
TATCAAC130350.034.7438772
ATCAACG132150.034.1261333
TCAACGC133650.033.8142364
CAACGCA137000.032.9167445
AACGCAG142300.031.952966
TGGTATC19650.031.1977732
ACGCAGA160550.028.081897
CGCAGAG162200.027.621028
TAAGGTG18350.026.9319925
TACATGG125800.025.1588062
AGGTAAG16150.025.0116772
GCAGAGT175950.024.7590359
GTAAGGT19200.024.7506584
GTACATG129400.024.7270491
GTGGTAT27250.024.1129261
ACATGGG132300.023.2398033
GGTAAGG20000.021.6221773
AGAGTAC163250.021.57760210-11