##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938570_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2587360 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.111926828891225 31.0 31.0 34.0 28.0 34.0 2 31.203384917444808 31.0 31.0 34.0 28.0 34.0 3 31.25910387421928 31.0 31.0 34.0 27.0 34.0 4 34.91102320512028 37.0 35.0 37.0 32.0 37.0 5 34.93639230721662 37.0 35.0 37.0 32.0 37.0 6 34.97477776575351 37.0 35.0 37.0 32.0 37.0 7 34.90556242656608 37.0 35.0 37.0 32.0 37.0 8 34.86299703172345 37.0 35.0 37.0 32.0 37.0 9 36.37380302702368 39.0 35.0 39.0 32.0 39.0 10-11 36.22848482623215 39.0 35.0 39.0 32.0 39.0 12-13 36.088070079154036 39.0 35.0 39.0 30.5 39.0 14-15 37.0711195968091 39.0 36.0 41.0 31.0 41.0 16-17 36.93770117030486 39.0 36.0 41.0 31.0 41.0 18-19 36.95456043998516 39.0 36.0 41.0 31.0 41.0 20-21 37.040350395770204 39.0 36.0 41.0 31.0 41.0 22-23 36.9641878207903 39.0 36.0 41.0 30.5 41.0 24-25 36.84313644023251 39.0 36.0 41.0 30.0 41.0 26-27 36.65904087564158 39.0 36.0 41.0 30.0 41.0 28-29 36.485418148228305 39.0 36.0 41.0 30.0 41.0 30-31 36.24256230288789 39.0 35.0 40.0 29.5 41.0 32-33 35.94887278925236 38.0 35.0 40.0 28.5 41.0 34-35 35.69871162111187 38.0 35.0 40.0 27.0 41.0 36-37 35.40192493506895 38.0 34.0 40.0 26.5 41.0 38-39 35.14130426380558 38.0 34.0 40.0 25.5 41.0 40-41 35.060361333560074 38.0 34.0 40.0 25.0 41.0 42-43 34.86389253911322 38.0 34.0 40.0 24.5 41.0 44-45 34.54367753231092 38.0 33.0 40.0 24.0 41.0 46-47 34.149663556675534 38.0 33.0 40.0 23.0 41.0 48-49 33.75329041957826 38.0 33.0 40.0 21.5 41.0 50-51 32.986399070867606 37.0 32.0 39.5 20.0 40.5 52-53 33.030245307958694 37.0 32.0 39.5 20.0 40.0 54-55 33.15930291880527 37.0 32.0 40.0 18.5 41.0 56-57 32.87322541122998 37.0 32.0 40.0 17.0 41.0 58-59 32.54820821223177 36.5 31.0 39.5 14.0 41.0 60-61 32.11535484045514 36.0 31.0 39.0 11.0 41.0 62-63 31.608943285820295 35.0 30.5 39.0 8.0 40.0 64-65 31.131946849298124 35.0 30.0 38.5 7.0 40.0 66-67 30.61591757621668 35.0 29.0 38.0 2.0 40.0 68-69 30.076498052068516 34.5 28.5 37.5 2.0 40.0 70-71 29.473314691422917 34.0 28.0 37.0 2.0 39.0 72-73 28.961581689444067 34.0 27.0 36.0 2.0 39.0 74-75 28.40020213654072 34.0 26.0 36.0 2.0 38.5 76-77 27.715978642322675 33.0 25.5 35.0 2.0 37.5 78-79 26.979429804897656 32.5 24.5 35.0 2.0 37.0 80-81 26.36962521643683 32.0 23.5 35.0 2.0 36.0 82-83 25.746671510729083 32.0 21.0 35.0 2.0 36.0 84-85 25.13752724785109 31.0 19.5 34.0 2.0 35.5 86-87 24.516851153299115 31.0 17.0 34.0 2.0 35.0 88-89 23.744100550367943 30.5 8.5 34.0 2.0 35.0 90-91 22.958364510543568 30.0 2.0 34.0 2.0 35.0 92-93 22.151273885350317 29.0 2.0 34.0 2.0 35.0 94-95 21.224597466143095 29.0 2.0 34.0 2.0 35.0 96-97 20.02108693803723 28.0 2.0 34.0 2.0 35.0 98-99 18.56546615082555 25.5 2.0 33.0 2.0 35.0 100-101 15.100380310432255 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6565354800513354 1101 2 0.5840924366174569 1101 3 0.6317594815026126 1101 4 0.5545573820337353 1101 5 0.4827914944116358 1101 6 0.5088930729655559 1101 7 0.5339156287197397 1101 8 0.5045532951340732 1101 9 0.5236911930632786 1101 10-11 0.6554780363372146 1101 12-13 0.6496479926321044 1101 14-15 0.9054037905212482 1101 16-17 0.7934600732007127 1101 18-19 0.6844955141155893 1101 20-21 0.7545384602934817 1101 22-23 0.9801173487612971 1101 24-25 0.9840718319426571 1101 26-27 0.978233841039966 1101 28-29 0.7999640578226206 1101 30-31 0.9317975023760141 1101 32-33 0.970068883514692 1101 34-35 0.7950273031472292 1101 36-37 0.7569325294266562 1101 38-39 0.7401230782908215 1101 40-41 0.7033449458711303 1101 42-43 0.7234950774282041 1101 44-45 0.6416769204656916 1101 46-47 0.8191602570795027 1101 48-49 0.8752997800475839 1101 50-51 0.6736571221348058 1101 52-53 0.7032732901403804 1101 54-55 0.803627477158642 1101 56-57 0.8035749586742824 1101 58-59 0.8209729822599741 1101 60-61 0.9151682327031523 1101 62-63 0.7325493038676143 1101 64-65 0.6697145733238621 1101 66-67 0.5620398422620099 1101 68-69 0.6867271129565324 1101 70-71 0.9246363893563014 1101 72-73 0.8110382878799989 1101 74-75 0.7609667880311335 1101 76-77 0.9407805972401881 1101 78-79 0.8818628388473897 1101 80-81 0.7796609134649479 1101 82-83 0.8837928437783482 1101 84-85 1.0818339145771176 1101 86-87 1.1417580234771982 1101 88-89 1.435090742381803 1101 90-91 1.471028490742352 1101 92-93 1.3715402198284252 1101 94-95 1.2770594573247145 1101 96-97 1.4091283519264586 1101 98-99 1.1684221633569258 1101 100-101 0.9369854622217293 1102 1 0.45350408727796676 1102 2 0.5450516284639981 1102 3 0.4996344007935143 1102 4 0.4949995452826954 1102 5 0.4764264686255899 1102 6 0.4687513518798454 1102 7 0.35597589695998977 1102 8 0.43004216749518775 1102 9 0.521852905485531 1102 10-11 0.5647061713236852 1102 12-13 0.6597365887433568 1102 14-15 0.6057314490175685 1102 16-17 0.6862995622201922 1102 18-19 0.6714632223041903 1102 20-21 0.5971897234419075 1102 22-23 0.5987882436310556 1102 24-25 0.5043645914172927 1102 26-27 0.46071894561443116 1102 28-29 0.4180532750874235 1102 30-31 0.3826153817904938 1102 32-33 0.4786253553625457 1102 34-35 0.5124647178407784 1102 36-37 0.48896411018071007 1102 38-39 0.5477098150279645 1102 40-41 0.4386851500344946 1102 42-43 0.5318339820729605 1102 44-45 0.617039845771977 1102 46-47 0.5877030798954479 1102 48-49 0.49548440976650454 1102 50-51 0.5253017896133301 1102 52-53 0.6196446091926262 1102 54-55 0.6242371066732417 1102 56-57 0.7857609287771865 1102 58-59 0.7855960809988716 1102 60-61 0.740697480044858 1102 62-63 0.6903863000998314 1102 64-65 0.7800323424018565 1102 66-67 0.6561135470342698 1102 68-69 0.6283099920032242 1102 70-71 0.38079324697319805 1102 72-73 0.5873931790555318 1102 74-75 0.6080937894123828 1102 76-77 0.7570974836263034 1102 78-79 0.6855974428211375 1102 80-81 0.6969987801294373 1102 82-83 0.7644319780864528 1102 84-85 0.6526130536256076 1102 86-87 0.6956564158337422 1102 88-89 0.8659153237161412 1102 90-91 0.7634877684061614 1102 92-93 0.8648449201627031 1102 94-95 0.77757468784624 1102 96-97 0.9659086515513131 1102 98-99 1.2601924509673665 1102 100-101 1.171490378325137 1103 1 0.6083216955198409 1103 2 0.5393901391955396 1103 3 0.5116319806004839 1103 4 0.4551884714046537 1103 5 0.4941037914326003 1103 6 0.4994860724443271 1103 7 0.4388023614088823 1103 8 0.464682399821136 1103 9 0.4537244716006228 1103 10-11 0.5023538632888247 1103 12-13 0.4017665925278564 1103 14-15 0.5800451441157293 1103 16-17 0.7135446728558961 1103 18-19 0.6848010351452203 1103 20-21 0.5725798379447369 1103 22-23 0.6811750449242382 1103 24-25 0.8544162447568979 1103 26-27 0.7465630980865967 1103 28-29 0.707216774233359 1103 30-31 0.700645583848285 1103 32-33 0.779425765756784 1103 34-35 0.8460653529447555 1103 36-37 0.9263291053458289 1103 38-39 0.7608327531234309 1103 40-41 0.9481530930985826 1103 42-43 0.7964885420046031 1103 44-45 0.7877640861767645 1103 46-47 0.6989862464143286 1103 48-49 0.7490037213490552 1103 50-51 0.6486885964141038 1103 52-53 0.7720900978064762 1103 54-55 0.8095875429138246 1103 56-57 0.8406584757746387 1103 58-59 0.8138966821918103 1103 60-61 0.8407830837516244 1103 62-63 0.8620673664954239 1103 64-65 0.7296989364207711 1103 66-67 0.7848897680869591 1103 68-69 0.9036362184454845 1103 70-71 0.8852161576093778 1103 72-73 0.9785676571915722 1103 74-75 0.8241924004765053 1103 76-77 0.9050418538056846 1103 78-79 0.8901135104323288 1103 80-81 1.0378334005344314 1103 82-83 1.2279161748396525 1103 84-85 0.9334108361119391 1103 86-87 1.1547330445268784 1103 88-89 1.1586166635557724 1103 90-91 1.2408167911312695 1103 92-93 1.2224391412830578 1103 94-95 1.1362303005146437 1103 96-97 1.1557415590953788 1103 98-99 1.0967446043175961 1103 100-101 0.7611929919919884 1104 1 0.5121970995378398 1104 2 0.5753278985441632 1104 3 0.4810901815245572 1104 4 0.5276164120973732 1104 5 0.48425737108487965 1104 6 0.5665538146815976 1104 7 0.5229456923580926 1104 8 0.39400029974236617 1104 9 0.5566119742789866 1104 10-11 0.5830915978908635 1104 12-13 0.6238974988862154 1104 14-15 0.6578966586362398 1104 16-17 0.7563380631063552 1104 18-19 0.7501889527813788 1104 20-21 0.6818020108750034 1104 22-23 0.7935220009447548 1104 24-25 0.8797751455261533 1104 26-27 0.7950815478646334 1104 28-29 0.8996085607850972 1104 30-31 0.81118196583882 1104 32-33 0.7610311393847837 1104 34-35 0.668854382366014 1104 36-37 0.6746283819612202 1104 38-39 0.7943341820871765 1104 40-41 0.8214917529810677 1104 42-43 0.7487551169278746 1104 44-45 0.7012874098227257 1104 46-47 0.7025550343452451 1104 48-49 0.6215253670565772 1104 50-51 0.45557395338343554 1104 52-53 0.44431893420446045 1104 54-55 0.5886724628948699 1104 56-57 0.6395909779852289 1104 58-59 0.5948252973795718 1104 60-61 0.647309992632934 1104 62-63 0.6993575110214465 1104 64-65 0.6614284201529124 1104 66-67 0.7270716614412969 1104 68-69 0.7928682145584602 1104 70-71 0.8275692399158814 1104 72-73 0.6571870699734816 1104 74-75 0.7718994191480952 1104 76-77 0.8552155325046193 1104 78-79 0.7125815134139906 1104 80-81 0.8854618790124569 1104 82-83 0.8501270548821438 1104 84-85 0.7481187510923668 1104 86-87 0.6712664720179049 1104 88-89 0.8763997175847855 1104 90-91 0.8009889910715167 1104 92-93 0.7908983501773363 1104 94-95 0.7083486826922822 1104 96-97 0.7679027649174621 1104 98-99 0.8038248405568389 1104 100-101 0.7426898730183051 1105 1 0.4932548639789509 1105 2 0.45533361425880514 1105 3 0.3712055675139112 1105 4 0.39521724087600063 1105 5 0.32771823630118746 1105 6 0.4461844366227936 1105 7 0.39386878027293193 1105 8 0.3266173825845087 1105 9 0.4666251918690989 1105 10-11 0.5934200728667562 1105 12-13 0.6001460253035376 1105 14-15 0.7032651436146224 1105 16-17 0.6486280999012664 1105 18-19 0.5961370469477743 1105 20-21 0.6845526985469164 1105 22-23 0.7920707452736906 1105 24-25 0.7638354463156389 1105 26-27 0.8549581241515654 1105 28-29 0.7857676005450429 1105 30-31 0.7607988343416565 1105 32-33 0.7732440140320236 1105 34-35 0.8953752983092826 1105 36-37 0.9359615724016876 1105 38-39 0.8170094937722396 1105 40-41 0.828444383995965 1105 42-43 1.0601633261230319 1105 44-45 0.975378253962333 1105 46-47 0.8751338933457049 1105 48-49 0.9208323366561189 1105 50-51 0.8663775471390167 1105 52-53 0.8148892553561708 1105 54-55 0.8816385615623901 1105 56-57 0.9302127405687415 1105 58-59 1.0444243042741448 1105 60-61 1.1431482379085338 1105 62-63 1.1733108207774592 1105 64-65 1.23362504073663 1105 66-67 1.4130824498526877 1105 68-69 1.410303823460687 1105 70-71 1.3489361887959568 1105 72-73 1.1871141946117838 1105 74-75 1.2677555260319622 1105 76-77 1.1408583717858676 1105 78-79 1.3799224557924425 1105 80-81 1.3593883963560778 1105 82-83 1.3928029024154576 1105 84-85 1.2699902904057687 1105 86-87 1.4670457739862535 1105 88-89 1.2525964453381526 1105 90-91 1.0599376568093781 1105 92-93 1.377879916997614 1105 94-95 1.2260897250957399 1105 96-97 1.3928402492885539 1105 98-99 1.5449922752726586 1105 100-101 1.1385879546815811 1106 1 0.31904292334695583 1106 2 0.42769336770607325 1106 3 0.1366639366533704 1106 4 0.4813001958701477 1106 5 0.4032169802558343 1106 6 0.16921570599754432 1106 7 0.23353886262868429 1106 8 0.29516337470966647 1106 9 0.38566704971893984 1106 10-11 0.16361910708912575 1106 12-13 0.231695846256315 1106 14-15 0.5580747156552306 1106 16-17 0.3294417762267514 1106 18-19 0.09722544428394286 1106 20-21 0.44139549488451024 1106 22-23 0.5188950242877368 1106 24-25 0.5531770124764108 1106 26-27 0.270552359959467 1106 28-29 0.3438047983628749 1106 30-31 0.39564852819748353 1106 32-33 0.5669026408903264 1106 34-35 0.44632487257288034 1106 36-37 0.5081185847506475 1106 38-39 0.4791508485501197 1106 40-41 0.6462497258202404 1106 42-43 0.12160529233644723 1106 44-45 0.6548464110736063 1106 46-47 0.25995601304767035 1106 48-49 0.8310703641432866 1106 50-51 0.4823227422289946 1106 52-53 0.7025143446523714 1106 54-55 0.5312867636849248 1106 56-57 0.11426623783847134 1106 58-59 -0.19387409184818694 1106 60-61 0.6614292623139377 1106 62-63 0.5088135814619505 1106 64-65 0.7790693072598778 1106 66-67 0.20904899074899674 1106 68-69 0.02958371448308128 1106 70-71 0.14165907110804454 1106 72-73 0.44176150554902804 1106 74-75 0.680618712461385 1106 76-77 0.5985037607761612 1106 78-79 0.8874894290059991 1106 80-81 0.6931638637166664 1106 82-83 0.5720102421435307 1106 84-85 0.5787017757799511 1106 86-87 0.7920763258644641 1106 88-89 0.601597536080714 1106 90-91 0.7615373414975082 1106 92-93 0.7436893022730757 1106 94-95 0.37280135089912037 1106 96-97 0.2123460209569359 1106 98-99 0.18315555804106154 1106 100-101 -0.3931677343354085 1107 1 0.4859815441912758 1107 2 0.4877075969673115 1107 3 0.4837274115317669 1107 4 0.4748051954448158 1107 5 0.4516544681725634 1107 6 0.38058403506467897 1107 7 0.3833762701191503 1107 8 0.4193929452798457 1107 9 0.4317961493314968 1107 10-11 0.4595669576200194 1107 12-13 0.42507073315687904 1107 14-15 0.5309941695299969 1107 16-17 0.5760110936088765 1107 18-19 0.6781471176141309 1107 20-21 0.7407556614596658 1107 22-23 0.7674788647962814 1107 24-25 0.7567540476861012 1107 26-27 0.7967469605735005 1107 28-29 0.7822381103007601 1107 30-31 0.7088237414430694 1107 32-33 0.7290630526109041 1107 34-35 0.7462882360918357 1107 36-37 0.6913275731584392 1107 38-39 0.7283446526348456 1107 40-41 0.7900213307107791 1107 42-43 0.8529119870491968 1107 44-45 0.8436958709030051 1107 46-47 0.8519445136392676 1107 48-49 0.7955257275694478 1107 50-51 0.7656542066829175 1107 52-53 0.746983202676823 1107 54-55 0.7717032789079141 1107 56-57 0.6890341423477508 1107 58-59 0.6068476178284854 1107 60-61 0.7094612356185976 1107 62-63 0.6706105283574146 1107 64-65 0.5294376053938343 1107 66-67 0.5659028160229482 1107 68-69 0.4744530604783961 1107 70-71 0.614024144458341 1107 72-73 0.6223721071219792 1107 74-75 0.6729347180602865 1107 76-77 0.753958439805789 1107 78-79 0.7137943257278572 1107 80-81 0.6914393391209224 1107 82-83 0.6475756426121642 1107 84-85 0.7355948475217211 1107 86-87 0.8590466532148078 1107 88-89 0.9907175645392385 1107 90-91 1.0451757210630497 1107 92-93 1.1156655842599115 1107 94-95 0.9632547926016848 1107 96-97 1.0459796638798124 1107 98-99 1.164872600674709 1107 100-101 0.8452361910847035 1108 1 0.3626319076934301 1108 2 0.3246106329670333 1108 3 0.40440362112567385 1108 4 0.36064042727369383 1108 5 0.46134768191395636 1108 6 0.40814514537922264 1108 7 0.35995852675326745 1108 8 0.27976536903467064 1108 9 0.30473130309636076 1108 10-11 0.32671070426777504 1108 12-13 0.366959245001901 1108 14-15 0.4628339975767517 1108 16-17 0.4603434604353893 1108 18-19 0.49175992807789015 1108 20-21 0.4082342336848015 1108 22-23 0.5245870710764464 1108 24-25 0.4330425021779618 1108 26-27 0.44630937726539344 1108 28-29 0.4234906925202182 1108 30-31 0.5560211371851267 1108 32-33 0.4743538543336214 1108 34-35 0.5758176083403441 1108 36-37 0.5985822822239513 1108 38-39 0.7180094022193018 1108 40-41 0.6184953120285215 1108 42-43 0.5149500419276691 1108 44-45 0.6253451855859424 1108 46-47 0.7069614540610232 1108 48-49 0.6867658965488275 1108 50-51 0.5825028899501277 1108 52-53 0.6199291765386548 1108 54-55 0.734936722168122 1108 56-57 0.7580524873906924 1108 58-59 0.721921274145032 1108 60-61 0.7749266059978268 1108 62-63 0.8721578686049867 1108 64-65 0.6359489140546906 1108 66-67 0.6059192519493841 1108 68-69 0.4159762115030503 1108 70-71 0.5039836255185222 1108 72-73 0.5567745058644604 1108 74-75 0.4771041063464416 1108 76-77 0.45251770738479635 1108 78-79 0.6465139670582367 1108 80-81 0.6742997744738339 1108 82-83 0.6993491428006884 1108 84-85 0.772257318911528 1108 86-87 0.8263091554053794 1108 88-89 0.7910016750608868 1108 90-91 0.9395274313021851 1108 92-93 0.866751178528844 1108 94-95 0.7682932404554528 1108 96-97 0.8332047803120197 1108 98-99 0.6525896664237258 1108 100-101 0.3594738455532056 1109 1 0.5316253972063159 1109 2 0.39802656472414455 1109 3 0.48487324185823866 1109 4 0.4374670607567097 1109 5 0.36368460413076775 1109 6 0.44414868015892495 1109 7 0.45850241392886915 1109 8 0.5131813007917785 1109 9 0.47918927348356277 1109 10-11 0.37053764433883174 1109 12-13 0.38170901506087773 1109 14-15 0.34122314796719166 1109 16-17 0.6002392205032052 1109 18-19 0.5264999594580786 1109 20-21 0.4965651323484437 1109 22-23 0.46475262861581257 1109 24-25 0.3067794496432015 1109 26-27 0.38914513625111624 1109 28-29 0.37404164925523986 1109 30-31 0.3409225781488985 1109 32-33 0.3493434844441694 1109 34-35 0.5893655532987694 1109 36-37 0.47065247284957223 1109 38-39 0.3446169154296328 1109 40-41 0.5275356914763023 1109 42-43 0.560837974983265 1109 44-45 0.6114607151716882 1109 46-47 0.6601786446325377 1109 48-49 0.7824628432926772 1109 50-51 0.6905372999485095 1109 52-53 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-0.9066974417434253 2316 62-63 -0.8382940832109682 2316 64-65 -0.8625818148446669 2316 66-67 -1.023418871610275 2316 68-69 -1.0391566770235485 2316 70-71 -1.5980290746193475 2316 72-73 -1.1951612084931362 2316 74-75 -0.697195725593101 2316 76-77 -0.6761743394306095 2316 78-79 -1.040587644108225 2316 80-81 -1.212098397994442 2316 82-83 -1.014276837132691 2316 84-85 -1.0132140051432579 2316 86-87 -1.2985708373488976 2316 88-89 -1.4201909504058108 2316 90-91 -1.346120004014665 2316 92-93 -1.2805291604098592 2316 94-95 -1.2953402871034392 2316 96-97 -1.2059744405717545 2316 98-99 -1.226735877760074 2316 100-101 -1.144777983488046 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7800.0 3 2504.0 4 2598.0 5 2988.0 6 3630.0 7 4549.0 8 6048.0 9 7727.0 10 9623.0 11 12159.0 12 15413.0 13 18469.0 14 20990.0 15 25647.0 16 29424.0 17 34708.0 18 31999.0 19 29087.0 20 29886.0 21 32086.0 22 35318.0 23 39932.0 24 44983.0 25 50614.0 26 57264.0 27 65263.0 28 75047.0 29 87678.0 30 101491.0 31 119904.0 32 141685.0 33 172997.0 34 212651.0 35 266061.0 36 315400.0 37 313554.0 38 151536.0 39 8647.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.76747701416902 18.532817486345568 14.894423540216783 22.805281959268637 2 15.663065708559431 19.998484187015748 39.87473661782681 24.46371348659801 3 19.168456020562004 24.325894029261494 29.73753436120163 26.768115588974872 4 13.061533850868628 16.38306634828256 36.451551155753634 34.10384864509518 5 13.867182816989649 37.69283125983735 33.536493400520534 14.903492522652476 6 32.331721239990486 35.76344963481348 17.186681182597127 14.718147942598906 7 28.960458342822214 33.1628106053254 19.88314856278764 17.993582489064753 8 27.46228838870943 33.75349181586024 19.47568149613073 19.308538299299606 9 27.33439252415949 16.3831352795997 19.59156323187502 36.69090896436579 10-11 25.366318973535485 26.362650902189337 26.80760247564366 21.463427648631512 12-13 26.490442761739498 23.760010393177623 27.131819362861094 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22.296533497870985 26.124933407870127 28.53686362473044 23.041669469528447 40-41 22.83695883499417 25.90264157085119 28.81113558030223 22.449264013852403 42-43 22.550229377570517 25.968954489031354 28.477533677931238 23.003282455466888 44-45 22.473418595329928 26.226219358768667 28.70397462006825 22.596387425833157 46-47 23.039978446338978 26.544013862993165 27.77217826686687 22.643829423800984 48-49 22.550676426556723 26.789131717929028 27.595667808636165 23.064524046878088 50-51 22.874172963616775 26.837395863088346 27.553448862992873 22.734982310302 52-53 23.245433228021735 26.389145773939738 27.66639435240712 22.699026645631402 54-55 22.54223811671864 26.757816501285824 27.855356217233545 22.844589164761995 56-57 22.681234443400598 26.971053394974458 27.431564491169837 22.916147670455107 58-59 22.58379357049205 26.347507916285284 27.686260761200305 23.382437752022366 60-61 22.216114922375446 26.67697563419284 28.06366125668131 23.043248186750407 62-63 22.36513444281144 26.631764777500454 28.157843117360986 22.84525766232712 64-65 22.164538022419084 26.761180455408628 27.82964481280905 23.24463670936324 66-67 22.087583575562437 26.751369067094092 27.788279042513985 23.372768314829486 68-69 22.814312097671316 26.291375062521116 27.85994961204054 23.034363227767027 70-71 22.597467865081665 26.40272542766019 27.524712477046485 23.475094230211653 72-73 22.490534732525497 26.51256791140958 27.631487390338584 23.36540996572634 74-75 22.44521519419917 26.959068781039765 27.44671704803188 23.14899897672919 76-77 22.57276463579269 26.50492989527536 27.609075684498297 23.31322978443365 78-79 22.64778774470243 26.824001323426188 27.484825434519777 23.0433854973516 80-81 22.87316969941181 26.787048820577635 27.199138829863816 23.140642650146734 82-83 22.714848841908182 26.81938854174296 27.357130281537028 23.108632334811823 84-85 22.65848771930706 26.96300631007554 27.274615933022872 23.103890037594528 86-87 22.871763513560524 26.898755460658908 26.970146980722358 23.259334045058214 88-89 22.748742882228463 26.83188924025491 26.847547032819314 23.571820844697314 90-91 22.563204671823645 26.8188705609614 27.08323425735936 23.534690509855594 92-93 22.814915727573435 26.440455713026278 27.285605793658053 23.459022765742237 94-95 22.56860501935234 26.24539485460905 27.508120018140282 23.677880107898332 96-97 22.926400197957022 25.812783693290342 27.43972363343927 23.821092475313367 98-99 22.617090326409038 26.058118475250165 27.040998768741893 24.283792429598904 100-101 23.217347306993894 25.485929112018567 27.43829444720164 23.8584291337859 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 528.0 1 370.0 2 291.0 3 388.0 4 377.0 5 400.5 6 659.0 7 1494.0 8 2462.5 9 2737.0 10 2364.0 11 2038.5 12 2057.0 13 2215.5 14 2383.5 15 2548.0 16 2811.0 17 3079.5 18 3453.0 19 3882.5 20 4374.0 21 5124.5 22 6035.5 23 7091.5 24 8511.5 25 10663.5 26 13769.0 27 17492.0 28 20468.0 29 23535.0 30 27557.5 31 33874.5 32 43217.0 33 47034.5 34 47282.0 35 50515.0 36 55521.0 37 60728.0 38 64368.5 39 66864.5 40 69801.5 41 74622.5 42 78426.5 43 81446.0 44 84207.5 45 88488.0 46 91495.5 47 93063.5 48 94479.0 49 93417.5 50 92288.0 51 91126.0 52 89434.0 53 90067.5 54 92481.5 55 88910.5 56 81748.5 57 75090.0 58 70838.5 59 64292.5 60 52824.5 61 44854.0 62 38514.0 63 29034.5 64 20618.5 65 15696.5 66 11760.5 67 9041.5 68 7133.0 69 5539.5 70 4294.0 71 3047.5 72 2284.5 73 1845.0 74 1405.5 75 1062.0 76 773.5 77 540.0 78 384.5 79 247.0 80 144.5 81 95.0 82 63.5 83 39.0 84 23.0 85 14.5 86 12.0 87 9.5 88 7.5 89 6.5 90 4.5 91 3.5 92 3.5 93 1.5 94 0.0 95 1.0 96 1.0 97 0.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18095665079463238 2 0.049819120648073714 3 0.047577453466081256 4 0.053529466328612954 5 0.05990662296703976 6 0.0655107909220209 7 0.038610784738111435 8 0.03691020963453095 9 0.04286222249706264 10-11 0.04342263929256076 12-13 0.04050460701255334 14-15 0.03590532434605158 16-17 0.03339311112485313 18-19 0.04133556984725743 20-21 0.05065008348277781 22-23 0.047558128748995114 24-25 0.06810030301156392 26-27 0.053084997835631684 28-29 0.04525848741574424 30-31 0.05376136293364665 32-33 0.04999304310184899 34-35 0.04894950837919733 36-37 0.04724893327561685 38-39 0.02775029373569971 40-41 0.025527951270793395 42-43 0.022300723517407705 44-45 0.004599282666501762 46-47 0.012696339125595202 48-49 0.01194267515923567 50-51 0.01008750231896605 52-53 0.010647919114464164 54-55 0.011633479685857399 56-57 0.009063292313400532 58-59 0.011131037041617711 60-61 0.011285634778306847 62-63 0.0041548141735205 64-65 0.007304743058561623 66-67 0.004077515305175933 68-69 0.00904396759631439 70-71 0.02357615484509307 72-73 0.020638797847999504 74-75 0.02152773483396203 76-77 0.013044184033145754 78-79 0.005256323047430586 80-81 0.007053521736441778 82-83 0.005855389277100983 84-85 0.005739440974584132 86-87 0.00842557664955785 88-89 0.030475078844845713 90-91 0.037142106239564654 92-93 0.023208985220456373 94-95 0.03289066848061344 96-97 0.036330468121946694 98-99 0.02222342464906314 100-101 0.12416130727846145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2587360.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.70665814268048 #Duplication Level Percentage of deduplicated Percentage of total 1 71.56550471755666 32.71020058933749 2 14.268175395025429 13.043012302004648 3 5.487010911791982 7.523787959113008 4 2.7974784679325766 5.114535679812152 5 1.557901326635966 3.560323167828925 6 0.9500903004893878 2.6055271541487013 7 0.6546194886958693 2.094432842835087 8 0.4505151804614618 1.6473234673152026 9 0.3295280292128516 1.355546248169673 >10 1.7206314786919106 14.179657985179183 >50 0.1358500769583815 4.2645948914932035 >100 0.07444322584939413 6.315128110877856 >500 0.004168233342299711 1.3565129178397195 >1k 0.0038279693959882306 3.3332526712250226 >5k 2.5519795973254867E-4 0.896164012820081 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9915 0.38320913981819305 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7639 0.29524302764207533 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5495 0.21237864077669902 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3859 0.14914816647084286 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3769 0.14566971739533732 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3566 0.13782388225836376 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3344 0.12924370787211675 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3237 0.12510821841568237 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3052 0.1179580730938099 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2952 0.11409312967658153 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2791 0.10787057077484385 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 3.864943417228372E-5 9 3.864943417228372E-5 0.0 0.0 0.0 3.864943417228372E-5 10-11 3.864943417228372E-5 0.0 0.0 0.0 3.864943417228372E-5 12-13 3.864943417228372E-5 0.0 0.0 3.864943417228372E-5 9.66235854307093E-5 14-15 3.864943417228372E-5 0.0 0.0 7.729886834456744E-5 1.1594830251685115E-4 16-17 3.864943417228372E-5 0.0 0.0 1.1594830251685115E-4 1.1594830251685115E-4 18-19 3.864943417228372E-5 0.0 0.0 1.1594830251685115E-4 1.1594830251685115E-4 20-21 3.864943417228372E-5 0.0 0.0 1.5459773668913488E-4 1.1594830251685115E-4 22-23 3.864943417228372E-5 0.0 0.0 3.285201904644116E-4 1.1594830251685115E-4 24-25 3.864943417228372E-5 0.0 0.0 8.116381176179581E-4 2.318966050337023E-4 26-27 3.864943417228372E-5 0.0 0.0 0.0016232762352359161 2.318966050337023E-4 28-29 3.864943417228372E-5 0.0 0.0 0.0051403747449137345 2.318966050337023E-4 30-31 3.864943417228372E-5 0.0 0.0 0.016599931976995858 2.318966050337023E-4 32-33 3.864943417228372E-5 0.0 0.0 0.03930647455321254 3.0919547337826975E-4 34-35 3.864943417228372E-5 0.0 0.0 0.07326000247356379 3.0919547337826975E-4 36-37 3.864943417228372E-5 0.0 0.0 0.1316206480737122 3.0919547337826975E-4 38-39 3.864943417228372E-5 0.0 0.0 0.21577979098386 3.0919547337826975E-4 40-41 3.864943417228372E-5 0.0 0.0 0.30666393543998516 3.0919547337826975E-4 42-43 3.864943417228372E-5 0.0 0.0 0.4018188423721477 3.0919547337826975E-4 44-45 3.864943417228372E-5 0.0 0.0 0.5198542143343021 3.0919547337826975E-4 46-47 3.864943417228372E-5 0.0 0.0 0.66096329849731 3.4784490755055347E-4 48-49 3.864943417228372E-5 0.0 0.0 0.8140343825366396 3.864943417228372E-4 50-51 3.864943417228372E-5 0.0 1.932471708614186E-5 0.9831836311916393 3.864943417228372E-4 52-53 3.864943417228372E-5 0.0 3.864943417228372E-5 1.1479268443509987 4.251437758951209E-4 54-55 3.864943417228372E-5 0.0 3.864943417228372E-5 1.323704470966545 4.637932100674046E-4 56-57 3.864943417228372E-5 1.932471708614186E-5 3.864943417228372E-5 1.5252805948920907 4.637932100674046E-4 58-59 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 1.738103704161771 4.637932100674046E-4 60-61 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 1.9715656112794506 4.637932100674046E-4 62-63 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 2.206844041803228 4.637932100674046E-4 64-65 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 2.454393667676705 4.637932100674046E-4 66-67 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 2.741230443386309 4.637932100674046E-4 68-69 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 3.0576147115206234 4.637932100674046E-4 70-71 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 3.394347906746645 4.637932100674046E-4 72-73 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 3.749961350565828 5.024426442396884E-4 74-75 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 4.102386216065797 5.410920784119721E-4 76-77 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 4.495122441407458 5.797415125842558E-4 78-79 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 4.924440356193186 5.797415125842558E-4 80-81 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 5.367652742563848 5.990662296703976E-4 82-83 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 5.83577468925855 6.570403809288232E-4 84-85 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 6.31566538865871 6.570403809288232E-4 86-87 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 6.804252210747634 6.570403809288232E-4 88-89 3.864943417228372E-5 3.864943417228372E-5 3.864943417228372E-5 7.322154628656236 6.570403809288232E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4805 0.0 59.753204 1 GTATCAA 9695 0.0 47.19684 1 TATCAAC 13035 0.0 34.743877 2 ATCAACG 13215 0.0 34.126133 3 TCAACGC 13365 0.0 33.814236 4 CAACGCA 13700 0.0 32.916744 5 AACGCAG 14230 0.0 31.95296 6 TGGTATC 1965 0.0 31.197773 2 ACGCAGA 16055 0.0 28.08189 7 CGCAGAG 16220 0.0 27.62102 8 TAAGGTG 1835 0.0 26.931992 5 TACATGG 12580 0.0 25.158806 2 AGGTAAG 1615 0.0 25.011677 2 GCAGAGT 17595 0.0 24.759035 9 GTAAGGT 1920 0.0 24.750658 4 GTACATG 12940 0.0 24.727049 1 GTGGTAT 2725 0.0 24.112926 1 ACATGGG 13230 0.0 23.239803 3 GGTAAGG 2000 0.0 21.622177 3 AGAGTAC 16325 0.0 21.577602 10-11 >>END_MODULE