FastQCFastQC Report
Thu 26 May 2016
SRR938570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938570_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2587360
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96290.37215540164491995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79050.30552377713190276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56720.21921959062519325No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39930.15432719064992886No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC37980.14679055098633356No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37430.14466483210685796No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33450.12928235730628904No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31570.12201626368189969No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG31270.1208567806567312No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA30740.11880836064560014No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT25970.10037258054542081No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51550.059.318951
GTATCAA100500.044.906711
TATCAAC138050.032.7953032
ATCAACG139250.032.258023
TCAACGC140850.031.8240854
CAACGCA144150.031.0955435
TAAGGTG18600.030.6668665
GTAAGGT17550.030.6057054
AACGCAG149350.030.11636
TGGTATC20700.030.0962282
AGGTAAG16800.028.8736722
GGTAAGG18400.028.1585163
ACGCAGA170400.026.256587
CGCAGAG172800.025.7819618
AAGGTAA18900.025.1622411
GTGGTAT27500.024.2106511
TACATGG127000.023.5161952
GTACATG129550.023.3836961
GCAGAGT189550.022.977489
ACCGTAC1050.0021980422.6171328
Produced by FastQC (version 0.11.5)